FastQCFastQC Report
Thu 26 May 2016
SRR1513008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042408
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32880.16098644345302213No Hit
GTCCTACAGTGGACATTTCTAAATT26850.13146246979056095No Hit
CTGTAGGACGTGGAATATGGCAAGA25020.12250245788304785No Hit
GTCCTAAAGTGTGTATTTCTCATTT23380.11447272043587765No Hit
CTTTAGGACGTGAAATATGGCGAGG22650.11089850803561287No Hit
TATCAACGCAGAGTACTTTTTTTTT22600.11065369896710157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT500.001487206913.3149236
TAGGACC6400.012.7837114
TAACGCT550.003036639212.108044
GGACCGA550.003043343312.1044756
GGCGAGG6150.011.88316619
GTCCTAA8500.011.1827021
GTAGGAC16850.010.9453943
AGGACCT12350.010.7844895
ATTAGGC1158.7400986E-710.7466983
TGTAGGA18950.010.68510152
AGGACGT15350.010.5360795
CTGTAGG18800.010.3142581
GTGCGCC1201.5386686E-610.2840411
GGACGTG15650.010.1487546
AAGTCGT1606.1227183E-910.0988827
GGACCTG12600.09.9635486
ATAGGAC3150.09.95939353
TGGCGAG16050.09.9346118
TAGGACG16650.09.8277184
GTCCTAC16200.09.7986691
Produced by FastQC (version 0.11.5)