##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513008_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2042408 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.525855754579887 31.0 31.0 34.0 27.0 34.0 2 30.67192059568901 31.0 31.0 34.0 27.0 34.0 3 30.760924849491385 31.0 31.0 34.0 27.0 34.0 4 34.24663044798101 37.0 35.0 37.0 30.0 37.0 5 33.90408527581169 37.0 35.0 37.0 30.0 37.0 6 33.756367483871976 37.0 35.0 37.0 29.0 37.0 7 33.645755402446525 37.0 35.0 37.0 28.0 37.0 8 33.531807063035394 37.0 35.0 37.0 28.0 37.0 9 35.00136897231111 39.0 35.0 39.0 28.0 39.0 10 34.897146897191945 39.0 35.0 39.0 27.0 39.0 11 34.91009729691619 39.0 35.0 39.0 27.0 39.0 12 34.76696918539293 39.0 35.0 39.0 27.0 39.0 13 34.746904144519604 39.0 35.0 39.0 27.0 39.0 14 35.8132478917043 40.0 36.0 41.0 27.0 41.0 15 35.82165708320766 40.0 36.0 41.0 27.0 41.0 16 35.79922718673252 40.0 36.0 41.0 27.0 41.0 17 35.77127390805363 40.0 36.0 41.0 27.0 41.0 18 35.70339080144613 40.0 36.0 41.0 27.0 41.0 19 35.68258056176827 40.0 36.0 41.0 27.0 41.0 20 35.654539641442845 40.0 36.0 41.0 27.0 41.0 21 35.60552690745434 40.0 36.0 41.0 27.0 41.0 22 35.563560268075726 40.0 36.0 41.0 27.0 41.0 23 35.459427793075626 40.0 36.0 41.0 26.0 41.0 24 35.437578583710994 40.0 36.0 41.0 26.0 41.0 25 35.34059747122025 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5222172723480725 1101 2 0.5746763683582294 1101 3 0.5542974784691275 1101 4 0.35485565782634154 1101 5 0.5230390719204863 1101 6 0.5031854258081978 1101 7 0.594778130417474 1101 8 0.5720507569457496 1101 9 0.49831246086065306 1101 10 0.7814065523557474 1101 11 0.7857446969109532 1101 12 0.7843729348238426 1101 13 0.8247225976819763 1101 14 0.9249300489018779 1101 15 0.830349334968183 1101 16 1.0404954964583766 1101 17 1.0556967263120995 1101 18 0.5085740061775539 1101 19 0.8129260458328815 1101 20 0.7912140205515357 1101 21 1.0342481848129594 1101 22 0.9442641209690592 1101 23 1.0249044442641377 1101 24 0.8371908303662465 1101 25 1.0366193866808828 1102 1 0.576499586487337 1102 2 0.6198131603859487 1102 3 0.5417820199172034 1102 4 0.4600555198335172 1102 5 0.7417116746170223 1102 6 0.7242052852383978 1102 7 0.7783173057511874 1102 8 0.6545940259381879 1102 9 0.9361437635171725 1102 10 1.0709882258705221 1102 11 0.9786517116239395 1102 12 0.9687583958625154 1102 13 1.0930400756613636 1102 14 1.1949403010429123 1102 15 1.1962548682756804 1102 16 1.0110206670207305 1102 17 1.0188798513670392 1102 18 1.0728149275381682 1102 19 1.1274283395124698 1102 20 1.1666705794228776 1102 21 1.1579798587180292 1102 22 1.1411670680769106 1102 23 1.2049976512601646 1102 24 1.1363968241938736 1102 25 1.2170199652937583 1103 1 0.4588312959359371 1103 2 0.43313020888616194 1103 3 0.47358277472959287 1103 4 0.4834261730652969 1103 5 0.5618304098752844 1103 6 0.6882118316552095 1103 7 0.6702755980275441 1103 8 0.6497346019911632 1103 9 0.6855013569859452 1103 10 0.7149960403204858 1103 11 0.7460596982563672 1103 12 0.7663655312102833 1103 13 0.807705808523373 1103 14 0.8084232505467241 1103 15 0.5382620962603113 1103 16 0.7762367957370557 1103 17 0.8570785280922593 1103 18 0.8420288829865683 1103 19 0.940845284759952 1103 20 0.9339025481823029 1103 21 0.5497871317687171 1103 22 0.8423305961169305 1103 23 0.8142063702013047 1103 24 0.8931148804477758 1103 25 0.9929206430750881 1104 1 0.4671297080095336 1104 2 0.4959337075336343 1104 3 0.45364026545207636 1104 4 0.4814504908364583 1104 5 0.4307436938561011 1104 6 0.5277799327001702 1104 7 0.5123593575037333 1104 8 0.4660030862425302 1104 9 0.5815601848109537 1104 10 0.6193765154995461 1104 11 0.651739982359409 1104 12 0.6572482609925601 1104 13 0.7317295655237288 1104 14 0.8113224863598276 1104 15 0.7903487806482659 1104 16 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2216 18 -2.2486361640462675 2216 19 -2.526458055130483 2216 20 -2.883618505546636 2216 21 -2.5848678952906496 2216 22 -3.018049111321602 2216 23 -2.0931847199996128 2216 24 -2.3699997721014796 2216 25 -2.2119663602699404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49543.0 3 3.0 4 813.0 5 7387.0 6 8430.0 7 6561.0 8 5325.0 9 4772.0 10 4110.0 11 3797.0 12 3497.0 13 3327.0 14 3161.0 15 3203.0 16 3315.0 17 3433.0 18 3926.0 19 4573.0 20 5774.0 21 7462.0 22 9553.0 23 11624.0 24 14132.0 25 15956.0 26 18204.0 27 20175.0 28 22672.0 29 27901.0 30 34833.0 31 44249.0 32 56669.0 33 73364.0 34 95680.0 35 124782.0 36 172009.0 37 266706.0 38 901487.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.8202900368671 19.15663323605332 13.33556116979054 27.687515557289043 2 13.79573894710454 20.553308206105935 39.780582313212946 25.870370533576576 3 16.8900631019855 24.77095663550084 30.23235318310543 28.10662707940823 4 11.450418316908054 17.324241027723076 37.69431301554994 33.53102763981893 5 12.750870900886335 38.04648724393554 36.072886197384605 13.12975565779352 6 31.806417534689047 37.50978760768818 17.934131237731805 12.749663619890967 7 27.47034121197695 31.642799558411966 22.627076555965996 18.259782673645088 8 25.006246295089678 36.853728915692166 20.578480411128695 17.561544378089465 9 26.171847448727693 14.868233897322163 19.866597963997712 39.093320689952435 10 14.40694663088206 29.02454277586852 34.96071273836527 21.607797854884147 11 34.23286767762478 22.215013761088315 24.769958963998878 18.782159597288032 12 22.82250609130295 24.721925165111525 32.011021145243305 20.444547598342226 13 28.74468196576187 21.81538556452075 25.870091360755758 23.56984110896162 14 20.878477893854463 21.77828438918724 27.59865212639693 29.74458559056136 15 23.045816163452045 30.462421462482176 23.333917631574824 23.15784474249095 16 22.365331608381815 27.850001689368682 27.359252329492506 22.425414372756997 17 21.83255198500673 28.331185910950285 27.591267899879675 22.24499420416331 18 21.85361267926302 27.482508237897758 29.088567805365283 21.57531127747394 19 23.35657097419868 26.931818626932554 27.367928334449683 22.343682064419088 20 23.611946203306378 27.146344711695708 27.66501040954662 21.576698675451293 21 22.966822969173137 26.619914639932198 27.170735313774387 23.242527077120283 22 23.13629889527081 27.661414188496774 27.04449322145847 22.157793694773943 23 22.44404643930106 27.429876890415628 27.36299505289834 22.76308161738497 24 23.065725881276357 26.889890212871382 27.825697816351003 22.21868608950126 25 22.832899195048864 27.084989913629332 27.543772890185863 22.53833800113594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 132.0 1 132.0 2 260.5 3 389.0 4 389.0 5 389.0 6 926.5 7 1464.0 8 1464.0 9 1464.0 10 2246.0 11 3028.0 12 3028.0 13 3028.0 14 5541.5 15 8055.0 16 8055.0 17 8055.0 18 15247.5 19 22440.0 20 22440.0 21 22440.0 22 36325.5 23 50211.0 24 50211.0 25 50211.0 26 72015.0 27 93819.0 28 93819.0 29 93819.0 30 120448.0 31 147077.0 32 147077.0 33 147077.0 34 168728.5 35 190380.0 36 190380.0 37 190380.0 38 208520.5 39 226661.0 40 226661.0 41 226661.0 42 239707.5 43 252754.0 44 252754.0 45 252754.0 46 261269.5 47 269785.0 48 269785.0 49 269785.0 50 260987.0 51 252189.0 52 252189.0 53 252189.0 54 226660.0 55 201131.0 56 201131.0 57 201131.0 58 172846.5 59 144562.0 60 144562.0 61 144562.0 62 118507.0 63 92452.0 64 92452.0 65 92452.0 66 71175.5 67 49899.0 68 49899.0 69 49899.0 70 36304.5 71 22710.0 72 22710.0 73 22710.0 74 15665.5 75 8621.0 76 8621.0 77 8621.0 78 5906.5 79 3192.0 80 3192.0 81 3192.0 82 2139.5 83 1087.0 84 1087.0 85 1087.0 86 690.5 87 294.0 88 294.0 89 294.0 90 180.5 91 67.0 92 67.0 93 67.0 94 37.5 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07657627663033047 2 9.792362740451468E-5 3 0.0 4 0.026194570330707673 5 0.0694278518298009 6 0.07647835300292596 7 0.03358780419974853 8 0.05865625281530429 9 0.037210978413715574 10 0.10046964171703204 11 0.0026928997536241534 12 0.007442195682743115 13 0.0031825178906467267 14 0.003916945096180587 15 0.0035252505865625283 16 0.011016408083007901 17 0.020808770823459367 18 8.813126466406321E-4 19 0.001566778038472235 20 0.0025460143125173814 21 4.896181370225734E-5 22 3.4273269591580133E-4 23 0.0 24 4.896181370225734E-5 25 0.002350167057708352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2042408.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.10996583248705 #Duplication Level Percentage of deduplicated Percentage of total 1 81.71179829539555 58.1052318489665 2 12.400765558362961 17.636360303037442 3 3.1879889125879455 6.800933479454288 4 1.1275041799376284 3.207071348454043 5 0.5464774429195248 1.942999614711615 6 0.28623036253453554 1.2212298780030721 7 0.16209560765153816 0.8068629185187973 8 0.11432198569350041 0.6503545997253509 9 0.07367390086105427 0.4715053715678046 >10 0.35521072941051907 4.2495777461422035 >50 0.017883267461156063 0.871131641546268 >100 0.013296281352456054 1.8672330388427156 >500 0.0017209223947423082 0.7967574096012019 >1k 0.0010325534368452434 1.3727508014286225 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3288 0.16098644345302213 No Hit GTCCTACAGTGGACATTTCTAAATT 2685 0.13146246979056095 No Hit CTGTAGGACGTGGAATATGGCAAGA 2502 0.12250245788304785 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2338 0.11447272043587765 No Hit CTTTAGGACGTGAAATATGGCGAGG 2265 0.11089850803561287 No Hit TATCAACGCAGAGTACTTTTTTTTT 2260 0.11065369896710157 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.8961813702257336E-5 0.0 0.0 0.0 0.0 2 4.8961813702257336E-5 0.0 0.0 0.0 0.0 3 4.8961813702257336E-5 0.0 0.0 0.0 0.0 4 4.8961813702257336E-5 0.0 0.0 0.0 0.0 5 4.8961813702257336E-5 0.0 0.0 0.0 0.0 6 4.8961813702257336E-5 0.0 0.0 0.0 4.8961813702257336E-5 7 4.8961813702257336E-5 0.0 0.0 0.0 4.8961813702257336E-5 8 9.792362740451467E-5 0.0 0.0 0.0 4.8961813702257336E-5 9 9.792362740451467E-5 0.0 0.0 1.46885441106772E-4 4.8961813702257336E-5 10 9.792362740451467E-5 0.0 0.0 1.46885441106772E-4 4.8961813702257336E-5 11 9.792362740451467E-5 0.0 0.0 1.46885441106772E-4 4.8961813702257336E-5 12 9.792362740451467E-5 0.0 0.0 1.46885441106772E-4 4.8961813702257336E-5 13 9.792362740451467E-5 0.0 0.0 1.46885441106772E-4 4.8961813702257336E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 50 0.0014872069 13.314923 6 TAGGACC 640 0.0 12.783711 4 TAACGCT 55 0.0030366392 12.10804 4 GGACCGA 55 0.0030433433 12.104475 6 GGCGAGG 615 0.0 11.883166 19 GTCCTAA 850 0.0 11.182702 1 GTAGGAC 1685 0.0 10.945394 3 AGGACCT 1235 0.0 10.784489 5 ATTAGGC 115 8.7400986E-7 10.746698 3 TGTAGGA 1895 0.0 10.6851015 2 AGGACGT 1535 0.0 10.536079 5 CTGTAGG 1880 0.0 10.314258 1 GTGCGCC 120 1.5386686E-6 10.28404 11 GGACGTG 1565 0.0 10.148754 6 AAGTCGT 160 6.1227183E-9 10.098882 7 GGACCTG 1260 0.0 9.963548 6 ATAGGAC 315 0.0 9.9593935 3 TGGCGAG 1605 0.0 9.93461 18 TAGGACG 1665 0.0 9.827718 4 GTCCTAC 1620 0.0 9.798669 1 >>END_MODULE