FastQCFastQC Report
Thu 26 May 2016
SRR1513007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222462
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55820.2511628995231414No Hit
CTGTAGGACGTGGAATATGGCAAGA51900.2335248026737915No Hit
GTCCTAAAGTGTGTATTTCTCATTT50460.22704550179035682No Hit
CTTTAGGACGTGAAATATGGCGAGG44880.20193821086704747No Hit
GTATCAACGCAGAGTACTTTTTTTT32500.14623422132751876No Hit
GTCCTACAGTGTGCATTTCTCATTT27790.1250415080212845No Hit
CTGAAGGACCTGGAATATGGCGAGA24350.10956317813307945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG651.9217077E-716.0715124
GTCGTAC400.00517983414.2912151
CCAACGA1600.012.46174819
TAGGACC8400.012.0971174
AGTCGTC2500.011.7744468
TCCAACG2300.011.56809718
GTCGAGG752.0854303E-411.39359819
AGGACGT28200.011.3824375
AAGTCGT2600.011.3197987
CCGTTCA600.00589668311.0783569
GGCGAGG13900.010.99741819
GTCCTAC27550.010.9626491
GGACGTG28550.010.9073216
TTAGGAC18700.010.8172153
GTCTTAC2400.010.7184111
AAATGTC8150.010.717187
GACGTGA16300.010.6589357
CAAGTCG2500.010.6333076
GGTATCA10450.010.5759551
GTCCTAA16700.010.554391
Produced by FastQC (version 0.11.5)