##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513007_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2222462 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.301481870106215 31.0 30.0 34.0 24.0 34.0 2 29.337583274764654 31.0 30.0 34.0 23.0 34.0 3 29.55707949112291 31.0 30.0 34.0 25.0 34.0 4 32.99540239608146 35.0 35.0 37.0 26.0 37.0 5 33.12931694670145 37.0 35.0 37.0 27.0 37.0 6 33.13580614651679 37.0 35.0 37.0 27.0 37.0 7 33.025128888592924 37.0 35.0 37.0 26.0 37.0 8 33.012635086674145 37.0 35.0 37.0 26.0 37.0 9 34.35333382528025 39.0 35.0 39.0 26.0 39.0 10 34.23658582238976 39.0 35.0 39.0 25.0 39.0 11 34.287123469377654 39.0 35.0 39.0 26.0 39.0 12 34.19708728428203 39.0 35.0 39.0 25.0 39.0 13 34.19552910240985 39.0 35.0 39.0 25.0 39.0 14 35.212950322660184 40.0 35.0 41.0 25.0 41.0 15 35.15642427182107 40.0 35.0 41.0 25.0 41.0 16 35.06251490464179 40.0 35.0 41.0 24.0 41.0 17 35.00646940195153 39.0 35.0 41.0 24.0 41.0 18 34.72293609519533 39.0 34.0 41.0 19.0 41.0 19 34.94809270079758 40.0 35.0 41.0 22.0 41.0 20 35.0301836431849 40.0 35.0 41.0 24.0 41.0 21 34.97153517135501 40.0 35.0 41.0 23.0 41.0 22 34.88519308766584 40.0 35.0 41.0 22.0 41.0 23 34.6242284457507 39.0 34.0 41.0 18.0 41.0 24 34.6307378933813 39.0 35.0 41.0 18.0 41.0 25 34.551211224308894 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5193965213924407 1101 2 0.7280002804379819 1101 3 0.7473588533350757 1101 4 0.7499672233565136 1101 5 0.48391959615209146 1101 6 0.6283902568208646 1101 7 0.703206026735991 1101 8 0.5839604041457847 1101 9 0.5634858376838494 1101 10 0.4884932010165812 1101 11 0.6913165230325546 1101 12 0.7598534542084181 1101 13 0.5699472358275557 1101 14 0.5476010625408989 1101 15 0.4478139248284734 1101 16 0.8137907502809583 1101 17 0.8128351946253218 1101 18 1.0739693752998534 1101 19 0.6139636287988708 1101 20 0.7404721374020156 1101 21 0.7342837183611621 1101 22 0.7934087909167644 1101 23 0.9945940433116434 1101 24 0.6900967643938856 1101 25 0.8412862397333996 1102 1 0.7943803693599065 1102 2 0.8634739250851986 1102 3 0.8569398382271558 1102 4 0.9540056474222922 1102 5 0.9377794844856453 1102 6 0.8384816282954333 1102 7 1.0014114936979936 1102 8 1.0119218348818606 1102 9 0.9796799171793253 1102 10 1.1550383650740912 1102 11 1.169176453715565 1102 12 1.1961052953384623 1102 13 1.1431030241034605 1102 14 1.169214460900811 1102 15 1.3369652291035266 1102 16 1.3266204931113634 1102 17 1.203644303691057 1102 18 1.563672533334163 1102 19 1.4535554808641535 1102 20 1.3580598165332916 1102 21 1.1509481793269316 1102 22 1.4910203067424845 1102 23 1.616035523042008 1102 24 1.588768377880477 1102 25 1.4861356256536666 1103 1 0.4556062629847695 1103 2 0.5535255976888926 1103 3 0.5929053625280218 1103 4 0.7265724082624843 1103 5 0.3248061396877091 1103 6 0.6309132705998195 1103 7 0.5334187000199009 1103 8 0.5316271126937622 1103 9 0.7581671628084479 1103 10 0.673893460680695 1103 11 0.7527031293901345 1103 12 0.9024078789686456 1103 13 0.7727088055389899 1103 14 1.0012421397281486 1103 15 1.0200818814394381 1103 16 1.0901145765572906 1103 17 0.9213679248678162 1103 18 1.3017793179224597 1103 19 1.2463808503928746 1103 20 1.2012411619863457 1103 21 0.9926356051141028 1103 22 1.229383243318658 1103 23 1.518889390298284 1103 24 1.3845451456813223 1103 25 1.2398733319141542 1104 1 0.5779214640864758 1104 2 0.6068013075191807 1104 3 0.5611544613169563 1104 4 0.9127235686983113 1104 5 0.6453482434586206 1104 6 0.6030873562249965 1104 7 0.8625079300413674 1104 8 0.8746638497021451 1104 9 0.8996171202793803 1104 10 0.9220236401128332 1104 11 0.8578391818831506 1104 12 0.8236600965554288 1104 13 0.9041816881992872 1104 14 0.9853518242030148 1104 15 1.1462678752018434 1104 16 1.003675757650086 1104 17 0.9527806187800891 1104 18 1.2239865357117594 1104 19 1.1342466205739257 1104 20 1.1402109866045933 1104 21 1.2284136439168094 1104 22 1.1270042891285357 1104 23 1.3846919744450688 1104 24 1.1339473895956118 1104 25 0.9891929479374895 1105 1 0.670169361929414 1105 2 0.7572535504607174 1105 3 0.740471494103776 1105 4 0.8945475811224597 1105 5 0.6092692022407817 1105 6 0.3924139782137459 1105 7 0.701977836326563 1105 8 0.763372129069964 1105 9 0.6442203753756672 1105 10 0.8731168645058531 1105 11 0.8582150779567073 1105 12 0.9238823037796138 1105 13 0.893876004600898 1105 14 0.9819479745104047 1105 15 1.1092927503893364 1105 16 1.0854422220225004 1105 17 0.980358341753508 1105 18 1.086597804841709 1105 19 1.0727332825630214 1105 20 1.1051695918921283 1105 21 1.148294333026442 1105 22 0.8182439444629708 1105 23 1.0137015693448248 1105 24 1.0602868463808477 1105 25 1.1550651155056002 1106 1 0.5538672720486986 1106 2 0.561095341813381 1106 3 0.6493749221654639 1106 4 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1107 17 1.3623350440956585 1107 18 1.6683480231168986 1107 19 1.4952846110560927 1107 20 1.5460585755792025 1107 21 1.4571557701187103 1107 22 1.3995016301182943 1107 23 1.5321378183490637 1107 24 1.5302791358291046 1107 25 1.5409663672780098 1108 1 0.3649670189603107 1108 2 0.41905311297516334 1108 3 0.3467030278976715 1108 4 0.3991827473397507 1108 5 0.39496956381222503 1108 6 0.4455024868310602 1108 7 0.5659612236441376 1108 8 0.4676437886248337 1108 9 0.6331966236200088 1108 10 0.569794516822995 1108 11 0.3636892327809065 1108 12 0.44845345984691676 1108 13 0.5679250367785684 1108 14 0.8280036739138055 1108 15 0.8157161226845702 1108 16 0.7514775559903129 1108 17 0.6698770966299108 1108 18 0.8613312912409725 1108 19 0.8544064201538504 1108 20 0.6687041970058019 1108 21 0.6073571170651846 1108 22 0.4301647886216031 1108 23 0.7163880006373589 1108 24 0.7122672134967729 1108 25 0.8137654942265513 1109 1 0.12472592560996532 1109 2 0.1988066487411615 1109 3 0.3330293639878157 1109 4 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-3.946643744839424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97324.0 3 3.0 4 781.0 5 4778.0 6 5913.0 7 5354.0 8 4915.0 9 4630.0 10 4208.0 11 3964.0 12 3875.0 13 3679.0 14 3886.0 15 4021.0 16 4039.0 17 4320.0 18 4805.0 19 5937.0 20 7309.0 21 9592.0 22 11844.0 23 14635.0 24 16730.0 25 19446.0 26 21647.0 27 24067.0 28 27697.0 29 32438.0 30 40024.0 31 50460.0 32 63753.0 33 81785.0 34 107239.0 35 141441.0 36 195106.0 37 304656.0 38 886161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.34188958110103 19.23830475305456 12.841776921656306 25.578028744188103 2 14.07608265093735 20.51918811082543 42.619815410986035 22.784913827251184 3 18.34135885656774 25.178627532000817 30.207925417997878 26.27208819343356 4 11.900135437498227 17.33545964354147 39.433917168424564 31.33048775053574 5 12.580433597577912 38.418574753787105 36.53133641196046 12.469655236674525 6 30.712616407094167 38.012088831444466 18.770091235529698 12.505203525931673 7 27.020933325232722 31.825899898967396 23.28286203536816 17.870304740431717 8 23.84219847383214 38.345563829236625 21.239071877510515 16.573165819420723 9 26.171166873389947 15.312164149683255 20.872270217626504 37.644398759300294 10 14.495577833903695 29.295387733867152 35.60374763500073 20.605286797228427 11 33.44164333576206 22.40923449280541 25.97837910331019 18.17074306812234 12 22.973606079958092 24.813849213474953 32.88796659209384 19.324578114473116 13 28.66658775845627 22.607292288762647 26.145740464295308 22.58037948848578 14 20.028814358029877 23.015344703347587 28.34951159168047 28.606329346942065 15 22.1074543834223 32.407394071420214 23.612373862398616 21.87277768275887 16 21.124282111946656 28.569030441018757 29.146221211907097 21.160466235127494 17 20.861280196226247 29.357921622016946 28.69916851378872 21.08162966796809 18 20.77198273512981 27.945245337084295 31.03114045340327 20.25163147438262 19 23.002041525419152 27.320389870145302 27.996307611433263 21.681260993002287 20 23.055645692286852 28.14004742717265 28.67656225457743 20.12774462596307 21 22.26547485631214 27.320317035956847 27.608412884874177 22.805795222856833 22 22.60630218574243 29.146652709214393 27.682335346992854 20.564709758050327 23 21.51542618689287 28.791738375902813 28.341860431623594 21.350975005580718 24 22.594396090044196 27.379231510067413 29.196226856643953 20.830145543244434 25 22.093555476245232 28.115564240685924 28.605272976277863 21.18560730679098 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 233.0 1 233.0 2 392.0 3 551.0 4 551.0 5 551.0 6 1126.5 7 1702.0 8 1702.0 9 1702.0 10 2704.5 11 3707.0 12 3707.0 13 3707.0 14 6925.5 15 10144.0 16 10144.0 17 10144.0 18 18864.0 19 27584.0 20 27584.0 21 27584.0 22 45321.5 23 63059.0 24 63059.0 25 63059.0 26 91527.5 27 119996.0 28 119996.0 29 119996.0 30 156844.0 31 193692.0 32 193692.0 33 193692.0 34 217179.0 35 240666.0 36 240666.0 37 240666.0 38 255088.0 39 269510.0 40 269510.0 41 269510.0 42 278405.0 43 287300.0 44 287300.0 45 287300.0 46 287667.0 47 288034.0 48 288034.0 49 288034.0 50 271177.5 51 254321.0 52 254321.0 53 254321.0 54 221352.5 55 188384.0 56 188384.0 57 188384.0 58 158348.0 59 128312.0 60 128312.0 61 128312.0 62 102746.0 63 77180.0 64 77180.0 65 77180.0 66 58796.0 67 40412.0 68 40412.0 69 40412.0 70 29054.0 71 17696.0 72 17696.0 73 17696.0 74 12159.0 75 6622.0 76 6622.0 77 6622.0 78 4465.0 79 2308.0 80 2308.0 81 2308.0 82 1527.0 83 746.0 84 746.0 85 746.0 86 481.5 87 217.0 88 217.0 89 217.0 90 136.0 91 55.0 92 55.0 93 55.0 94 34.0 95 13.0 96 13.0 97 13.0 98 15.5 99 18.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3519970195215936 2 0.039505737330941995 3 0.035996116019081544 4 0.03478124710343754 5 0.0409005868266814 6 0.018493004604803142 7 0.018313024024707737 8 0.025062295778285525 9 0.022047621061687443 10 0.02132769874130581 11 0.02002283953561411 12 0.02128270359628196 13 0.02119271330623426 14 0.02938182970057531 15 0.030686688906267016 16 0.022182606496759003 17 0.024162392877808483 18 0.10038416854821364 19 0.026727116144168048 20 0.02380243171761767 21 0.02843693165507442 22 0.02119271330623426 23 0.023667446282546113 24 0.01988785410054255 25 0.02380243171761767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2222462.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.05722769081783 #Duplication Level Percentage of deduplicated Percentage of total 1 84.11140919680919 60.608349638900286 2 11.098981348716624 15.99523652361228 3 2.6607734851476255 5.751838825589199 4 0.9024668478089514 2.60117036543937 5 0.4043720120402001 1.4568963071687413 6 0.20854002010368666 0.9016089426755445 7 0.12734560554908725 0.6423319906132958 8 0.08335410836246322 0.48050127721912916 9 0.058556004692491455 0.3797445026512308 >10 0.3024778269679274 3.8604497870192036 >50 0.020949195671004773 1.0457308964439285 >100 0.01696071060516261 2.5500311392774764 >500 0.00206311538266915 1.0792888416390094 >1k 0.0015629661989912545 1.934232644820148 >5k 1.8755594387895056E-4 0.7125883169313654 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5582 0.2511628995231414 No Hit CTGTAGGACGTGGAATATGGCAAGA 5190 0.2335248026737915 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5046 0.22704550179035682 No Hit CTTTAGGACGTGAAATATGGCGAGG 4488 0.20193821086704747 No Hit GTATCAACGCAGAGTACTTTTTTTT 3250 0.14623422132751876 No Hit GTCCTACAGTGTGCATTTCTCATTT 2779 0.1250415080212845 No Hit CTGAAGGACCTGGAATATGGCGAGA 2435 0.10956317813307945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 4.499514502385193E-5 0.0 12 0.0 0.0 0.0 4.499514502385193E-5 0.0 13 0.0 0.0 0.0 8.999029004770386E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAACG 65 1.9217077E-7 16.071512 4 GTCGTAC 40 0.005179834 14.291215 1 CCAACGA 160 0.0 12.461748 19 TAGGACC 840 0.0 12.097117 4 AGTCGTC 250 0.0 11.774446 8 TCCAACG 230 0.0 11.568097 18 GTCGAGG 75 2.0854303E-4 11.393598 19 AGGACGT 2820 0.0 11.382437 5 AAGTCGT 260 0.0 11.319798 7 CCGTTCA 60 0.005896683 11.078356 9 GGCGAGG 1390 0.0 10.997418 19 GTCCTAC 2755 0.0 10.962649 1 GGACGTG 2855 0.0 10.907321 6 TTAGGAC 1870 0.0 10.817215 3 GTCTTAC 240 0.0 10.718411 1 AAATGTC 815 0.0 10.71718 7 GACGTGA 1630 0.0 10.658935 7 CAAGTCG 250 0.0 10.633307 6 GGTATCA 1045 0.0 10.575955 1 GTCCTAA 1670 0.0 10.55439 1 >>END_MODULE