FastQCFastQC Report
Thu 26 May 2016
SRR1513007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222462
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT52470.23608952594015106No Hit
CTGTAGGACGTGGAATATGGCAAGA50060.22524569598940275No Hit
CTTTAGGACGTGAAATATGGCGAGG47120.21201712335239026No Hit
GTCCTAAAGTGTGTATTTCTCATTT46210.20792256515521976No Hit
GTATCAACGCAGAGTACTTTTTTTT38830.17471614812761702No Hit
GTCCTACAGTGTGCATTTCTCATTT28110.12648135266204777No Hit
TATCAACGCAGAGTACTTTTTTTTT26290.11829223626770671No Hit
CTGAAGGACCTGGAATATGGCGAGA24940.1122178916894867No Hit
GGTATCAACGCAGAGTACTTTTTTT23450.10551361508093277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG402.7287935E-416.651214
GGCGTGC1051.9839717E-812.6717738
TAGGACC9350.012.4152664
CTACCGT550.003033155812.1099724
TCCAACG1850.011.79802818
AGCGCTC658.0183794E-411.69359710
GGCGAGG10700.011.61903819
GGTATCA12250.011.5634081
TGTACCG700.001472358710.8742615
GGACCTG19300.010.74482256
ATAGGAC3100.010.7343
AGGACCT20050.010.6776575
GAACAGT4950.010.5695756
TGGCGAG28250.010.41349618
AGGACGT28700.010.244355
TGTAGGA30850.010.2307822
GGACGTG28900.010.2038476
CACCTTT8100.010.19541414
CGTGCGC750.002645915610.1344510
AATGTCC7600.010.1290838
Produced by FastQC (version 0.11.5)