##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513007_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2222462 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.4739649091863 31.0 31.0 34.0 27.0 34.0 2 30.632796871217597 31.0 31.0 34.0 27.0 34.0 3 30.729781206607807 31.0 31.0 34.0 27.0 34.0 4 34.18998300083421 37.0 35.0 37.0 30.0 37.0 5 33.84538993242629 37.0 35.0 37.0 30.0 37.0 6 33.714793323800365 37.0 35.0 37.0 29.0 37.0 7 33.585183458704805 37.0 35.0 37.0 28.0 37.0 8 33.475177978296145 37.0 35.0 37.0 28.0 37.0 9 34.928080660096775 39.0 35.0 39.0 28.0 39.0 10 34.82171258721184 39.0 35.0 39.0 27.0 39.0 11 34.8412611779189 39.0 35.0 39.0 27.0 39.0 12 34.701668689948356 39.0 35.0 39.0 27.0 39.0 13 34.68523736288854 39.0 35.0 39.0 27.0 39.0 14 35.74320910773727 40.0 36.0 41.0 27.0 41.0 15 35.75745052108878 40.0 36.0 41.0 27.0 41.0 16 35.74319470929087 40.0 36.0 41.0 27.0 41.0 17 35.71934368281662 40.0 36.0 41.0 27.0 41.0 18 35.650379174087114 40.0 36.0 41.0 27.0 41.0 19 35.63685903291035 40.0 36.0 41.0 27.0 41.0 20 35.61581255382544 40.0 36.0 41.0 27.0 41.0 21 35.559491680847636 40.0 36.0 41.0 27.0 41.0 22 35.52458804694973 40.0 36.0 41.0 26.0 41.0 23 35.41490833139104 40.0 36.0 41.0 26.0 41.0 24 35.40051483444936 40.0 36.0 41.0 26.0 41.0 25 35.30312509280249 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5152282524612026 1101 2 0.572632630732695 1101 3 0.5443341448687242 1101 4 0.3405529431166272 1101 5 0.5162742484272442 1101 6 0.6055135534685121 1101 7 0.527451133570608 1101 8 0.6262555834731316 1101 9 0.4711662774677947 1101 10 0.7057259515135144 1101 11 0.743880224874907 1101 12 0.743580031570211 1101 13 0.8717370156082538 1101 14 0.9185978006643865 1101 15 0.9252140864807359 1101 16 1.0008967227737884 1101 17 0.9810543629430626 1101 18 0.5087379666015508 1101 19 0.7254312893512633 1101 20 0.795974734811665 1101 21 0.8811996778670235 1101 22 0.8076574734301047 1101 23 0.7988580909949619 1101 24 0.7181763848003726 1101 25 0.8788295587711872 1102 1 0.755977101778555 1102 2 0.676316705799124 1102 3 0.6748856734446491 1102 4 0.6198879457243933 1102 5 0.8217338244839709 1102 6 0.8006530457741405 1102 7 0.9183572934756086 1102 8 0.8219589789668973 1102 9 1.075489753712894 1102 10 1.178788293695618 1102 11 1.0197294638324763 1102 12 0.9986503077214479 1102 13 1.1064762752943693 1102 14 1.299033057293272 1102 15 1.2984297689888749 1102 16 1.2309712401727353 1102 17 1.2661773767724256 1102 18 1.3103564721043384 1102 19 1.3112873773252076 1102 20 1.2595749982857853 1102 21 1.2296820170090328 1102 22 1.175553761626226 1102 23 1.206088958304278 1102 24 1.2149614812197527 1102 25 1.2024754537853681 1103 1 0.5430545287940731 1103 2 0.37427395920779816 1103 3 0.549117995436216 1103 4 0.579708197126763 1103 5 0.5397160543492632 1103 6 0.5500040673521625 1103 7 0.5120246562074584 1103 8 0.609706184469907 1103 9 0.588155765259387 1103 10 0.7963222575821192 1103 11 0.7293929777333048 1103 12 0.8045568712066853 1103 13 0.8775555857803852 1103 14 0.9273052597254079 1103 15 0.6538196064371817 1103 16 0.776021152140963 1103 17 0.914320602420382 1103 18 0.8774280030382897 1103 19 0.8944408801708832 1103 20 0.9789293773546959 1103 21 0.540188998133388 1103 22 0.893494555140542 1103 23 0.8882783699244712 1103 24 0.888884095149244 1103 25 0.9746971300741123 1104 1 0.5884976006074325 1104 2 0.6709635780663987 1104 3 0.6101100161741968 1104 4 0.6508463558209172 1104 5 0.6951682800368175 1104 6 0.8490765287722297 1104 7 0.7652980168133965 1104 8 0.6957991813511128 1104 9 0.9451080111988759 1104 10 0.9872017585744572 1104 11 0.9588548433414701 1104 12 0.9704927940873205 1104 13 0.8866280472004533 1104 14 1.0727321299915147 1104 15 1.1216596161581123 1104 16 1.0283431578925146 1104 17 0.9944823748696479 1104 18 0.9909893461465558 1104 19 1.0455043497322265 1104 20 1.0974009233873474 1104 21 0.9310101735556415 1104 22 0.948761111096637 1104 23 0.8582319524916073 1104 24 0.9445722153647225 1104 25 1.0578633174450118 1105 1 0.4678604808380662 1105 2 0.5088673844635494 1105 3 0.4213483136603067 1105 4 0.424157269141638 1105 5 0.43474011322274464 1105 6 0.5223949937198782 1105 7 0.5335124245953082 1105 8 0.5605553211809209 1105 9 0.6365778266398436 1105 10 0.6414193422127425 1105 11 0.6810057713930888 1105 12 0.6603236416567242 1105 13 0.760342775946576 1105 14 0.8885634377206344 1105 15 0.868330058905741 1105 16 0.9133088477456681 1105 17 0.7238635242593645 1105 18 0.8951290260458507 1105 19 0.9914880679109643 1105 20 0.8664137491957078 1105 21 0.9293983305501925 1105 22 1.0287015423226222 1105 23 0.9110569069832266 1105 24 0.9995401947812823 1105 25 1.0728698687421812 1106 1 0.43599043959859785 1106 2 0.489932096690751 1106 3 0.5031322731293244 1106 4 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1107 17 0.90496476117724 1107 18 0.9844020909072526 1107 19 1.0691665800387185 1107 20 1.0665109384905094 1107 21 1.0254973404778909 1107 22 1.090859127209903 1107 23 1.1542427257256662 1107 24 1.1837122420514063 1107 25 1.1716249205342848 1108 1 0.49719794180129995 1108 2 0.49782593252630747 1108 3 0.4543497908781262 1108 4 0.46549474520902834 1108 5 0.4873869826427608 1108 6 0.4225999539558316 1108 7 0.36161109311684925 1108 8 0.47215754633761264 1108 9 0.29318856125716763 1108 10 0.5772472158922852 1108 11 0.5394785808461506 1108 12 0.6213284109509871 1108 13 0.4957267920060673 1108 14 0.5935109295419707 1108 15 0.6232370605783686 1108 16 0.5627348819908775 1108 17 0.581343392859516 1108 18 0.5809054905313431 1108 19 0.5503864054627101 1108 20 0.6312384379439138 1108 21 0.8345992260377741 1108 22 0.8035645940510463 1108 23 0.8125941008861162 1108 24 0.8024716437873778 1108 25 0.7456198649268089 1109 1 0.2998630929236725 1109 2 0.26537853728654426 1109 3 0.2868807787783325 1109 4 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-2.440380095003917 2216 24 -2.6825580642337883 2216 25 -2.581250354318101 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57373.0 3 1.0 4 988.0 5 8326.0 6 9590.0 7 7274.0 8 5843.0 9 5291.0 10 4646.0 11 4119.0 12 3751.0 13 3547.0 14 3460.0 15 3585.0 16 3584.0 17 3765.0 18 4179.0 19 5040.0 20 6166.0 21 8153.0 22 10245.0 23 12733.0 24 14903.0 25 17192.0 26 19553.0 27 22095.0 28 24599.0 29 29858.0 30 37275.0 31 47696.0 32 61122.0 33 78508.0 34 102650.0 35 134639.0 36 185647.0 37 291608.0 38 983458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.74706852887882 20.036807749530787 13.801780317085207 27.414343404505185 2 13.019182806072013 20.82949561724198 41.48805444779859 24.663267128887416 3 16.64140939192661 25.29613554697448 30.667610964776898 27.394844096322007 4 11.095698605808375 17.91039133411136 38.642921406874684 32.35098865320558 5 12.163990568029908 38.61844241018624 36.69602788347546 12.521539138308396 6 30.817015037478868 38.4571116188548 18.365411547076217 12.360461796590112 7 26.814337901023183 32.00538148402209 23.165812362829772 18.014468252124956 8 23.901697564770757 38.517710430676466 20.898137109061242 16.68245489549153 9 25.86232586363131 15.11134779197313 20.7384052761626 38.28792106823296 10 14.197616223839473 29.626623413184415 35.48214915514724 20.69361120782887 11 33.391004394399864 22.364597497990886 25.854019170490655 18.390378937118594 12 22.81409988430546 24.928236533465693 32.777870527411416 19.479793054817435 13 28.46530610408281 22.526857649396025 26.25747795704126 22.7503582894799 14 19.93577928810045 22.937215614560536 28.18678183676996 28.94022326056906 15 21.83956715855104 32.53164010155071 23.56522725166019 22.063565488238062 16 21.068086209410456 28.642055978874264 29.001711875664903 21.288145936050373 17 20.77112071157662 29.461902359395157 28.677657644439424 21.089319284588793 18 20.61370689457346 28.04517039689457 30.979887321484732 20.361235387047234 19 22.884140758056063 27.306441924140394 28.012842001534384 21.79657531626916 20 22.90847158663949 28.177936167244876 28.644015172990994 20.269577073124637 21 22.067093402158157 27.30373722020793 27.704915377326145 22.924254000307766 22 22.404788591324255 29.080127373001584 27.797038312240236 20.718045723433924 23 21.23703352408275 28.67023148202309 28.517967911262375 21.574767082631784 24 22.515120618485266 27.424135935732536 29.154469232769785 20.906274213012416 25 21.95544260418504 28.06381592446337 28.67140450469584 21.30933696665575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 126.0 1 126.0 2 258.5 3 391.0 4 391.0 5 391.0 6 1033.0 7 1675.0 8 1675.0 9 1675.0 10 2641.5 11 3608.0 12 3608.0 13 3608.0 14 6888.5 15 10169.0 16 10169.0 17 10169.0 18 19112.0 19 28055.0 20 28055.0 21 28055.0 22 45996.5 23 63938.0 24 63938.0 25 63938.0 26 92916.5 27 121895.0 28 121895.0 29 121895.0 30 157778.0 31 193661.0 32 193661.0 33 193661.0 34 216797.0 35 239933.0 36 239933.0 37 239933.0 38 254746.5 39 269560.0 40 269560.0 41 269560.0 42 278417.0 43 287274.0 44 287274.0 45 287274.0 46 287941.5 47 288609.0 48 288609.0 49 288609.0 50 271877.0 51 255145.0 52 255145.0 53 255145.0 54 221218.5 55 187292.0 56 187292.0 57 187292.0 58 157550.5 59 127809.0 60 127809.0 61 127809.0 62 102341.0 63 76873.0 64 76873.0 65 76873.0 66 58249.0 67 39625.0 68 39625.0 69 39625.0 70 28408.5 71 17192.0 72 17192.0 73 17192.0 74 11882.0 75 6572.0 76 6572.0 77 6572.0 78 4370.0 79 2168.0 80 2168.0 81 2168.0 82 1414.5 83 661.0 84 661.0 85 661.0 86 421.5 87 182.0 88 182.0 89 182.0 90 112.5 91 43.0 92 43.0 93 43.0 94 23.5 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07802158147135924 2 1.3498543507155578E-4 3 0.0 4 0.027042082159335006 5 0.06771769326089715 6 0.07847153292159775 7 0.034646261668365985 8 0.062903212743345 9 0.044950149878828075 10 0.10668348885155292 11 0.00395957276209897 12 0.010393878500509794 13 0.004364529067313637 14 0.005669388273005343 15 0.005579397982957639 16 0.01520835901806195 17 0.026052188968810265 18 0.0014848397857871135 19 0.0030146747165980792 20 0.004139553342194378 21 3.599611601908154E-4 22 5.84936885310075E-4 23 0.0 24 0.0 25 0.0031496601516696346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2222462.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.37682097103009 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39683150511961 59.526007118920845 2 11.564826383872994 16.50921084725496 3 2.8475406399167906 6.097451953892196 4 0.9433036984910921 2.693200768340369 5 0.41257366789695693 1.4724098415421158 6 0.21519000160997562 0.9215746931802543 7 0.13065129178705676 0.6527831704462995 8 0.08288075729327336 0.4732611980212295 9 0.06055551516362039 0.3890034148178008 >10 0.3038081547553023 3.8079359257174863 >50 0.021150760326466997 1.059112120161042 >100 0.01678022064183752 2.535896249311621 >500 0.0020167241936229555 1.047217056484986 >1k 0.0017646336694191998 2.353719298260354 >5k 1.260452621013714E-4 0.4612163436484866 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5247 0.23608952594015106 No Hit CTGTAGGACGTGGAATATGGCAAGA 5006 0.22524569598940275 No Hit CTTTAGGACGTGAAATATGGCGAGG 4712 0.21201712335239026 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4621 0.20792256515521976 No Hit GTATCAACGCAGAGTACTTTTTTTT 3883 0.17471614812761702 No Hit GTCCTACAGTGTGCATTTCTCATTT 2811 0.12648135266204777 No Hit TATCAACGCAGAGTACTTTTTTTTT 2629 0.11829223626770671 No Hit CTGAAGGACCTGGAATATGGCGAGA 2494 0.1122178916894867 No Hit GGTATCAACGCAGAGTACTTTTTTT 2345 0.10551361508093277 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.499514502385193E-5 0.0 2 0.0 0.0 0.0 4.499514502385193E-5 0.0 3 0.0 0.0 0.0 4.499514502385193E-5 0.0 4 0.0 0.0 0.0 4.499514502385193E-5 0.0 5 0.0 0.0 0.0 4.499514502385193E-5 0.0 6 0.0 0.0 0.0 4.499514502385193E-5 0.0 7 0.0 0.0 0.0 4.499514502385193E-5 0.0 8 0.0 0.0 0.0 4.499514502385193E-5 0.0 9 0.0 0.0 0.0 4.499514502385193E-5 0.0 10 0.0 0.0 0.0 4.499514502385193E-5 0.0 11 0.0 0.0 0.0 4.499514502385193E-5 0.0 12 0.0 0.0 0.0 8.999029004770386E-5 0.0 13 0.0 0.0 0.0 8.999029004770386E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGACG 40 2.7287935E-4 16.65121 4 GGCGTGC 105 1.9839717E-8 12.671773 8 TAGGACC 935 0.0 12.415266 4 CTACCGT 55 0.0030331558 12.109972 4 TCCAACG 185 0.0 11.798028 18 AGCGCTC 65 8.0183794E-4 11.693597 10 GGCGAGG 1070 0.0 11.619038 19 GGTATCA 1225 0.0 11.563408 1 TGTACCG 70 0.0014723587 10.874261 5 GGACCTG 1930 0.0 10.7448225 6 ATAGGAC 310 0.0 10.734 3 AGGACCT 2005 0.0 10.677657 5 GAACAGT 495 0.0 10.569575 6 TGGCGAG 2825 0.0 10.413496 18 AGGACGT 2870 0.0 10.24435 5 TGTAGGA 3085 0.0 10.230782 2 GGACGTG 2890 0.0 10.203847 6 CACCTTT 810 0.0 10.195414 14 CGTGCGC 75 0.0026459156 10.13445 10 AATGTCC 760 0.0 10.129083 8 >>END_MODULE