FastQCFastQC Report
Thu 26 May 2016
SRR1513006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992386
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT39950.20051335433997228No Hit
GTCCTACAGTGGACATTTCTAAATT38980.19564481982908938No Hit
CTGTAGGACGTGGAATATGGCAAGA34700.1741630386882863No Hit
CTTTAGGACGTGAAATATGGCGAGG33480.16803972724160882No Hit
GTCCTACAGTGTGCATTTCTCATTT24910.12502597388257095No Hit
GTATCAACGCAGAGTACTTTTTTTT21110.10595336445849347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1001.0149961E-813.29323619
TAGGACC7900.012.8640964
TCCAACG1650.012.66849218
CAGGACG751.4826195E-512.663724
AGGACCT15250.011.0233895
GGACCTG14150.011.0047246
GGCGAGG10000.010.91944319
TGGCGAG21350.010.81420418
AGGACGT21250.010.7266475
TATGTCG1252.236302E-710.64153316
GGACGTG21100.010.5749226
TCGAACT1355.7316356E-810.55018619
TTAGGAC13800.010.5301593
TGTAGGA24800.010.49352552
GACGTGA12000.010.3652987
CTAGGAC2300.010.3236863
TTTAGGA15000.010.3209322
GTCCTAC20700.010.3135391
CTGTAGG24700.010.3025411
GTAGGAC23800.010.2959243
Produced by FastQC (version 0.11.5)