##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513006_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1992386
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.646419920637868	31.0	30.0	34.0	18.0	34.0
2	28.667278830507744	31.0	30.0	34.0	16.0	34.0
3	28.898521672005323	31.0	30.0	34.0	20.0	34.0
4	32.30582276727502	35.0	33.0	37.0	22.0	37.0
5	32.444275356281366	35.0	33.0	37.0	24.0	37.0
6	32.42577241558614	35.0	33.0	37.0	23.0	37.0
7	32.29548591487794	35.0	33.0	37.0	22.0	37.0
8	32.29254070245425	36.0	33.0	37.0	22.0	37.0
9	33.566656260383276	38.0	34.0	39.0	19.0	39.0
10	33.42997943169647	38.0	33.0	39.0	18.0	39.0
11	33.47852675134236	38.0	33.0	39.0	19.0	39.0
12	33.37885379640291	38.0	33.0	39.0	18.0	39.0
13	33.373888895023356	38.0	33.0	39.0	18.0	39.0
14	34.308676631937786	39.0	33.0	41.0	17.0	41.0
15	34.246311206764155	39.0	33.0	41.0	17.0	41.0
16	34.14594762259924	39.0	33.0	41.0	17.0	41.0
17	34.062189756402624	39.0	33.0	41.0	17.0	41.0
18	33.76964855203761	39.0	33.0	41.0	11.0	41.0
19	33.98285071266311	39.0	33.0	41.0	16.0	41.0
20	34.06050785339789	39.0	34.0	41.0	15.0	41.0
21	33.98469222329408	39.0	34.0	41.0	10.0	41.0
22	33.88665198410348	39.0	34.0	41.0	10.0	41.0
23	33.614647964802	39.0	33.0	41.0	2.0	41.0
24	33.606185749147	39.0	33.0	41.0	2.0	41.0
25	33.5059606923558	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8649692105640874
1101	2	1.1076522739151002
1101	3	1.1087712505941703
1101	4	1.0704152228278687
1101	5	0.9794686669762172
1101	6	1.0548609162747198
1101	7	1.1028766858083543
1101	8	0.8735162892996655
1101	9	0.874785833060244
1101	10	0.9453539066788963
1101	11	1.223105447731136
1101	12	1.3521492404002444
1101	13	1.2288399855791567
1101	14	1.1502316554735117
1101	15	0.9980797072496301
1101	16	1.4297248149583766
1101	17	1.453309321093684
1101	18	1.600839102495975
1101	19	1.0059881989913464
1101	20	1.1629276743723267
1101	21	1.3051931170921023
1101	22	1.437994222778812
1101	23	1.6386681408611636
1101	24	1.3113925201255654
1101	25	1.5859552593050168
1102	1	0.7269137253132811
1102	2	0.6800389179861348
1102	3	0.7968730685295675
1102	4	0.8974982556190838
1102	5	0.9396957664372181
1102	6	0.7564931102261667
1102	7	0.9902975544601986
1102	8	0.9774624992347256
1102	9	1.0393736980552717
1102	10	1.0524086418792606
1102	11	1.0848896847614498
1102	12	1.1422038077248402
1102	13	1.0745219077207366
1102	14	1.1682932396970926
1102	15	1.4768329498958224
1102	16	1.3602132615633593
1102	17	1.1886718121030881
1102	18	1.4602147586617704
1102	19	1.574699544569519
1102	20	1.517440734542184
1102	21	1.282628457969743
1102	22	1.6032183728628269
1102	23	1.7893367946566698
1102	24	1.7355610858761423
1102	25	1.5086985238674657
1103	1	0.4866300994791217
1103	2	0.4436570651553673
1103	3	0.5309068009072675
1103	4	0.6339809035917057
1103	5	0.23244331316077194
1103	6	0.5671543422143586
1103	7	0.4456064553947314
1103	8	0.5361916756720149
1103	9	0.6596124903689145
1103	10	0.668001442192292
1103	11	0.6568006258610097
1103	12	0.9210828547439434
1103	13	0.7902091402964544
1103	14	0.9422862496481557
1103	15	1.0716571936943922
1103	16	1.1096036343091455
1103	17	0.891199877157483
1103	18	1.283186972503664
1103	19	1.1932353251599963
1103	20	1.2170228460111048
1103	21	0.9244208592251937
1103	22	1.000320408468788
1103	23	1.364907382376586
1103	24	1.1678629257987865
1103	25	1.1001099056112764
1104	1	0.40395641819714
1104	2	0.32554246676922816
1104	3	0.41901777399182905
1104	4	0.6059190100185958
1104	5	0.3001567969901018
1104	6	0.3853620793772734
1104	7	0.6585699004648049
1104	8	0.6758098882856558
1104	9	0.6838950167650282
1104	10	0.8327429364304706
1104	11	0.7028770237489326
1104	12	0.6873604782402509
1104	13	0.7466537452609856
1104	14	0.870206375667216
1104	15	0.925783587327544
1104	16	0.7876669461406465
1104	17	0.9551951926306046
1104	18	1.1147178872336951
1104	19	0.9255842252714785
1104	20	0.9451333877896957
1104	21	1.1447176715631855
1104	22	0.9633457323465464
1104	23	1.3199691854143012
1104	24	1.148251423684414
1104	25	0.8476651584323491
1105	1	0.4836153447830469
1105	2	0.6187039359929081
1105	3	0.6314915796100244
1105	4	0.7449740240469538
1105	5	0.3961542848998505
1105	6	0.3454593403913364
1105	7	0.5373724797308341
1105	8	0.5678150402190667
1105	9	0.5375213531232887
1105	10	0.6730669531047226
1105	11	0.4508679126262507
1105	12	0.5310475039443858
1105	13	0.5968947254212082
1105	14	0.5796549214084621
1105	15	0.8579999267136955
1105	16	0.6911633380320907
1105	17	0.6332855781978992
1105	18	0.7475775085575762
1105	19	0.7973592435736165
1105	20	0.7950890339360015
1105	21	0.7932823647447194
1105	22	0.3599260593383704
1105	23	0.7261482445448166
1105	24	0.7636254889038128
1105	25	0.8631978789545371
1106	1	0.8264046160118816
1106	2	0.7970625316659223
1106	3	0.888995540864471
1106	4	0.8516999351354784
1106	5	0.7672441032302473
1106	6	0.8219994960511983
1106	7	0.6676628720694211
1106	8	0.9165873004759746
1106	9	1.0605925460837184
1106	10	1.2699208700130455
1106	11	1.0793259133636894
1106	12	1.1154010471494615
1106	13	1.0561623168788472
1106	14	1.319249777258662
1106	15	1.3044233374783403
1106	16	1.3293824676523087
1106	17	1.0858352309030153
1106	18	1.41858536130853
1106	19	1.0423802706098044
1106	20	1.18683467655611
1106	21	1.1443728308274146
1106	22	1.2148033850182571
1106	23	1.3933331130510993
1106	24	1.3579656286189987
1106	25	1.3660592001699214
1107	1	0.5340116828152581
1107	2	0.5767868790119302
1107	3	0.7165548909709472
1107	4	0.8142811566586516
1107	5	0.6843625061618042
1107	6	0.5409927435167248
1107	7	0.7754304101548115
1107	8	0.7670471765885054
1107	9	0.9583565653281383
1107	10	0.6227332126510845
1107	11	0.6874157590807144
1107	12	0.8365192498711025
1107	13	0.8791555158086339
1107	14	1.185356361738151
1107	15	1.2764826784843706
1107	16	1.1343491903373106
1107	17	1.31281109044059
1107	18	1.61146865712778
1107	19	1.3443976888725828
1107	20	1.2501958067107566
1107	21	1.2113741412923247
1107	22	1.2976645060724579
1107	23	1.3520383009147352
1107	24	1.312681785559306
1107	25	1.4708744003495084
1108	1	0.2232265388338952
1108	2	0.2839629481223831
1108	3	0.28054622292320985
1108	4	0.3061038309399464
1108	5	0.23418306835154112
1108	6	0.3879953467051749
1108	7	0.38908504082369433
1108	8	0.4855466843669518
1108	9	0.6274617530358668
1108	10	0.6036175469598675
1108	11	0.34489366534343446
1108	12	0.5539772385931201
1108	13	0.4130367970692035
1108	14	0.5154723543274571
1108	15	0.605471022618083
1108	16	0.566429458063844
1108	17	0.5008702754896532
1108	18	0.5832602011139443
1108	19	0.5399170482123097
1108	20	0.4582477902548874
1108	21	0.4831138014532712
1108	22	0.355043461932965
1108	23	0.5907462587088972
1108	24	0.6529783855942455
1108	25	0.7065116904243212
1109	1	0.3527881195340825
1109	2	0.3864015357057937
1109	3	0.5833813383837061
1109	4	0.7690088767267866
1109	5	0.6407014331676066
1109	6	0.4988592108709824
1109	7	0.6772843079023119
1109	8	0.6919149641438409
1109	9	0.9358006298856623
1109	10	1.0846989582030986
1109	11	0.5240578014840978
1109	12	0.9538676998657962
1109	13	0.8524024026421699
1109	14	0.6833189360379279
1109	15	1.0273583887848616
1109	16	0.914150460069159
1109	17	0.7759535536772972
1109	18	-0.8989967829930947
1109	19	0.6782935437797164
1109	20	0.7907875660710246
1109	21	0.9740449173028267
1109	22	1.089517611391571
1109	23	0.47556096051981456
1109	24	0.9348992463725665
1109	25	1.0034232039365136
1110	1	0.3744197514997083
1110	2	0.3057844677429564
1110	3	0.5103191793131572
1110	4	0.23925270173656799
1110	5	0.4893853150578735
1110	6	0.4846775678829758
1110	7	0.5312086888024865
1110	8	0.39960065648123333
1110	9	0.862448398109855
1110	10	0.9505680085062238
1110	11	0.7731795311506033
1110	12	0.25035132049775655
1110	13	0.5373306954736492
1110	14	0.6587515715702494
1110	15	0.7585398738143851
1110	16	0.823177757955122
1110	17	0.49812156941991503
1110	18	0.8929472190883274
1110	19	0.7881972508278849
1110	20	0.7754825663863656
1110	21	0.7415412291942474
1110	22	0.7273030142927226
1110	23	0.7074513300095688
1110	24	1.0053339716241467
1110	25	0.9173045908157746
1111	1	0.34590237019726544
1111	2	0.6512785381992039
1111	3	0.5427828009706346
1111	4	0.5239295736064378
1111	5	0.6045599621365483
1111	6	0.5446594514490073
1111	7	0.6315382372106129
1111	8	0.4459313027374918
1111	9	0.6746288874475397
1111	10	0.763738597814033
1111	11	0.6065748689813688
1111	12	0.6632918660080236
1111	13	0.720051031267765
1111	14	0.5663380707536518
1111	15	0.7806561797687621
1111	16	0.6595085787808728
1111	17	0.8755335379043032
1111	18	0.8809844567006806
1111	19	0.628687868687507
1111	20	0.5435572006632796
1111	21	0.7324621608522293
1111	22	0.46549047865145354
1111	23	0.8960194528444205
1111	24	0.9287119300921276
1111	25	0.2879134892046906
1112	1	-0.26861864653541545
1112	2	0.1196007451422254
1112	3	0.10219978203618396
1112	4	0.22718972349929345
1112	5	0.13487202996051906
1112	6	0.13231248828754616
1112	7	-0.21761317964116955
1112	8	0.1372422383337124
1112	9	0.32401967840068835
1112	10	0.35617392567036177
1112	11	0.3131846891961416
1112	12	0.28036862243018135
1112	13	0.14947602411788807
1112	14	0.2583379414681346
1112	15	0.23315442657873575
1112	16	0.44369793897425325
1112	17	0.4462541871526824
1112	18	0.3580571386700555
1112	19	0.39999921564675134
1112	20	0.27469591227429646
1112	21	0.3067812571072608
1112	22	0.37044309354906346
1112	23	0.6789140968978771
1112	24	0.2797056656209662
1112	25	0.4804954385952911
1113	1	0.06907843645249656
1113	2	0.10370476502602699
1113	3	-0.091329886379512
1113	4	0.38078089428287853
1113	5	0.0470110027601649
1113	6	0.1753070835665156
1113	7	0.19621507939565674
1113	8	0.40500559253231927
1113	9	0.31907769802092645
1113	10	0.06331762098022864
1113	11	0.08902908114331609
1113	12	0.39834544306443576
1113	13	0.27306718417181486
1113	14	0.43608887560630905
1113	15	-0.03600427015085472
1113	16	-0.04241608621292414
1113	17	-0.05108773653549292
1113	18	-0.11084145177549942
1113	19	-0.06363960162170201
1113	20	-0.1635710810810238
1113	21	-0.04444455203778119
1113	22	0.12275589452190871
1113	23	-0.3378955618962536
1113	24	0.4653338228821653
1113	25	0.23473157854233762
1114	1	-1.3458119527592736
1114	2	-1.0620865682929512
1114	3	-0.8867191666617096
1114	4	-1.118329417294035
1114	5	-1.256244532589534
1114	6	-1.6142546886066924
1114	7	-1.62977595912227
1114	8	-1.2778953594049298
1114	9	-1.2211501170256724
1114	10	-1.2832850220501584
1114	11	-1.9117832856236383
1114	12	-1.3649935213734743
1114	13	-1.4168651446977378
1114	14	-1.7524350246154796
1114	15	-1.719416132271661
1114	16	-2.510634928032392
1114	17	-4.701852242176059
1114	18	-3.468515772959872
1114	19	-2.529049416186755
1114	20	-1.5815836991923007
1114	21	-2.7346431023056645
1114	22	-2.8535132083315524
1114	23	-2.6837465209784135
1114	24	-2.2386084070743983
1114	25	-2.054698061686846
1115	1	-1.2005849601874914
1115	2	-0.6541702162475751
1115	3	-0.7192906841642603
1115	4	-0.7033990347172896
1115	5	-0.5041070977748134
1115	6	-0.597342953568738
1115	7	-2.06777738037335
1115	8	-1.3228393369152371
1115	9	-1.1294662474019859
1115	10	-0.7947073188940053
1115	11	-1.5877166301008074
1115	12	-1.1947595913507385
1115	13	-1.0506583123172604
1115	14	-0.9700766536030727
1115	15	-2.463525886451219
1115	16	-1.086502644635253
1115	17	-1.1419740119383235
1115	18	-1.262226037011196
1115	19	-1.2831598044122785
1115	20	-1.1286665525308877
1115	21	-1.9396770978739326
1115	22	-1.8977753805730195
1115	23	-1.1292552111135663
1115	24	-1.1838128374911463
1115	25	-1.587822022613146
1116	1	-2.0941355719876213
1116	2	-3.115725437470136
1116	3	-2.70195715558949
1116	4	-2.3620697330888234
1116	5	-2.829538850037448
1116	6	-2.742526056315466
1116	7	-2.3645529865264123
1116	8	-3.00689973673213
1116	9	-3.2650403303638953
1116	10	-4.404042908282172
1116	11	-2.7764210199382156
1116	12	-2.7738390710908227
1116	13	-3.397317335620432
1116	14	-3.375105167629428
1116	15	-2.844769281847615
1116	16	-2.892536128541618
1116	17	-3.258354845449883
1116	18	-3.1206498196354175
1116	19	-3.5785661029194955
1116	20	-3.833291973058305
1116	21	-4.415002537478966
1116	22	-3.5120976333389287
1116	23	-3.490809246721721
1116	24	-4.25894291247895
1116	25	-3.9680976795012626
1201	1	0.42216601530759235
1201	2	0.419866672644865
1201	3	0.6380773413427399
1201	4	0.6718615747309116
1201	5	0.7508556388587948
1201	6	0.7848765457977436
1201	7	0.5079518449045324
1201	8	0.23879674621100122
1201	9	0.5109883304380034
1201	10	0.7430672207714721
1201	11	0.29769411447654903
1201	12	-0.29731964478334305
1201	13	0.02039629165824408
1201	14	0.6882459709607289
1201	15	0.3321406608685251
1201	16	0.831793711599218
1201	17	0.9372196320669488
1201	18	1.216266247778556
1201	19	0.7768811907497266
1201	20	0.7421431970285397
1201	21	0.713029880589481
1201	22	1.0194621609643804
1201	23	1.213243373212805
1201	24	0.8955305060513936
1201	25	0.7233551413865271
1202	1	0.7538876578005116
1202	2	0.8859270557870431
1202	3	0.6908941612303074
1202	4	1.0079197967032982
1202	5	1.0076117162164664
1202	6	0.9932646550822213
1202	7	0.9403419582356563
1202	8	0.8983897391386222
1202	9	1.2873617945277545
1202	10	1.4736733857916562
1202	11	1.1513512429028339
1202	12	1.2101252509569207
1202	13	1.1602849085899791
1202	14	1.444762730972947
1202	15	1.020330550875542
1202	16	1.2721230967372108
1202	17	1.459527583166583
1202	18	1.8730899210251835
1202	19	1.591879631627009
1202	20	1.454161945636642
1202	21	1.4927207548441785
1202	22	1.6046790427325064
1202	23	1.8103379705498668
1202	24	1.8497016945291307
1202	25	1.6256582547981466
1203	1	0.5228097047763534
1203	2	0.7069581263181384
1203	3	0.789775592270157
1203	4	0.9988324281580105
1203	5	0.7482853825789206
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2211	18	0.395159105226945
2211	19	0.248960730895476
2211	20	0.09248765800692382
2211	21	0.21026002315967673
2211	22	0.009140672245536052
2211	23	0.5790059549793298
2211	24	0.44504028794472816
2211	25	0.47366373094859426
2212	1	-0.041726887034766236
2212	2	-0.08050792518386984
2212	3	0.03367202506028377
2212	4	-0.21360379088640258
2212	5	-0.22544480835823322
2212	6	-0.2458793247549167
2212	7	-0.2273337242546063
2212	8	-0.28483327044807893
2212	9	-0.11800929273424288
2212	10	-0.049481370279664816
2212	11	-0.3163897521022889
2212	12	-0.13959949804446836
2212	13	-0.26698519745331595
2212	14	-0.2458457149719422
2212	15	-0.3226006003210884
2212	16	-0.13111170142292394
2212	17	-0.16185545635178755
2212	18	-2.8927636264102183
2212	19	-1.257120971599413
2212	20	-0.6503586802558985
2212	21	-0.6627545007284823
2212	22	-0.28808642674596285
2212	23	-0.4548841406707993
2212	24	-0.838968450620392
2212	25	-0.4739772104549971
2213	1	-0.05567592770895402
2213	2	0.132627554997228
2213	3	-0.24927794260073455
2213	4	-0.3064820178273635
2213	5	-0.15083570699807325
2213	6	-0.14571551869668298
2213	7	0.020106026399368204
2213	8	-0.41432862879662125
2213	9	-0.254090959698388
2213	10	-0.2514214212927257
2213	11	-0.22212507529058456
2213	12	-0.32648800820581414
2213	13	-0.0071283334324974135
2213	14	-0.11788094047156505
2213	15	-0.37653472859840775
2213	16	-0.9710463133455534
2213	17	-0.6449644320025527
2213	18	-0.3802945019809485
2213	19	-0.08086949139528343
2213	20	-0.7905105487197304
2213	21	-0.4507139397767048
2213	22	-0.3626163599760446
2213	23	-0.782917335627836
2213	24	-0.5307697990695672
2213	25	-0.5266952202929573
2214	1	-1.6696394892822788
2214	2	-3.8590705369411005
2214	3	-1.9297587545744541
2214	4	-1.5181925309898183
2214	5	-1.5653399321285093
2214	6	-1.6898069295478138
2214	7	-1.8980026933425087
2214	8	-1.4599497219068844
2214	9	-1.693044258873357
2214	10	-1.792088275128524
2214	11	-1.482468722068404
2214	12	-2.0059928820474404
2214	13	-1.8915930828917276
2214	14	-3.054718058662491
2214	15	-3.407879177225798
2214	16	-2.370329008921342
2214	17	-2.858178678976472
2214	18	-2.2447169469627966
2214	19	-4.4015850730127255
2214	20	-2.765910250525277
2214	21	-2.6561496078322477
2214	22	-2.7351843842569856
2214	23	-2.829245098552608
2214	24	-2.988521153387108
2214	25	-2.7764844423287194
2215	1	-0.8027053990951991
2215	2	-2.5801368818238117
2215	3	-1.2026577960234697
2215	4	-0.791472142054193
2215	5	-0.6988059757230616
2215	6	-1.237219754883597
2215	7	-1.8808113809502842
2215	8	-1.7639790784041907
2215	9	-1.354430114540115
2215	10	-3.0295556798300716
2215	11	-2.076137757862444
2215	12	-2.367148905341839
2215	13	-1.721233796294392
2215	14	-2.977239534993114
2215	15	-2.8259213911068564
2215	16	-2.43026196904491
2215	17	-2.6151863770100867
2215	18	-1.5645738304283725
2215	19	-2.2838387205651998
2215	20	-2.4377466316762266
2215	21	-2.0640254424535556
2215	22	-1.814171794250175
2215	23	-2.4505222007455636
2215	24	-2.2102879652944196
2215	25	-1.9172404669273995
2216	1	-2.162797104233551
2216	2	-2.088250976831425
2216	3	-2.7246521550869964
2216	4	-3.4023464398942203
2216	5	-2.56908779780823
2216	6	-2.627429672475536
2216	7	-2.6093047931550046
2216	8	-3.286492701697508
2216	9	-3.530899372086896
2216	10	-2.979594092371901
2216	11	-3.851971744711694
2216	12	-4.23442174498382
2216	13	-4.611042880934463
2216	14	-3.895588191943979
2216	15	-3.7900061080605347
2216	16	-2.896722704271017
2216	17	-2.9965247421202648
2216	18	-3.0286215838121677
2216	19	-3.282946124279537
2216	20	-3.235129936207546
2216	21	-3.7611509778339034
2216	22	-4.34793653680039
2216	23	-4.303555933995153
2216	24	-4.023752049124248
2216	25	-4.235880337667272
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108869.0
3	1.0
4	832.0
5	5243.0
6	6460.0
7	5765.0
8	4935.0
9	4995.0
10	4477.0
11	4188.0
12	4166.0
13	4130.0
14	4183.0
15	4165.0
16	4294.0
17	4662.0
18	5191.0
19	6310.0
20	7886.0
21	10105.0
22	12765.0
23	15426.0
24	17935.0
25	20167.0
26	22555.0
27	25027.0
28	28848.0
29	33667.0
30	41195.0
31	50951.0
32	63840.0
33	79901.0
34	102004.0
35	133302.0
36	177484.0
37	271451.0
38	695011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95423647451658	18.70016738268982	12.361889132516417	25.983707010277186
2	14.593530388815356	20.35074523445612	41.501756387365255	23.55396798936326
3	18.50720654408228	24.878618962454475	29.785827113235076	26.828347380228173
4	12.209786607393452	16.90180304978558	38.73195556919005	32.15645477363091
5	12.907950631169848	38.16162366811613	36.14318567540697	12.787240025307048
6	31.462294081267117	37.326008208637546	18.4527682063886	12.758929503706737
7	27.642947788616386	31.47451678065275	22.89624388069412	17.986291550036746
8	24.51374779797773	37.483652698729216	20.94743812373897	17.055161379554086
9	26.546990157034628	14.962592514687639	20.26063501609704	38.22978231218069
10	14.779069732417799	28.853616441079406	35.178861698827205	21.188452127675596
11	34.080571163798446	22.083701681172165	25.345594359044636	18.490132795984746
12	23.332662313572385	24.48487519785461	32.26125794618756	19.92120454238544
13	29.100782229701032	22.057301262350666	25.70027680750683	23.141639700441466
14	20.609708219242727	22.34659646015389	27.745830735230477	29.297864585372906
15	22.70907773694941	31.528305704317738	23.20159533529102	22.56102122344183
16	22.01532455105933	27.83183657158979	28.220648233630374	21.932190643720503
17	21.653041676205163	28.687181464390278	27.97912374342848	21.680653115976078
18	21.693774193580794	27.31462178896617	30.00104485124433	20.99055916620871
19	23.58560756672089	26.908147077133158	27.355764403478123	22.15048095266783
20	23.655488066604715	27.44763749346739	27.951520409090474	20.94535403083741
21	22.90819051903142	26.812272323813275	26.976540567026664	23.302996590128643
22	23.284928069815226	28.296019931716902	26.93959490984191	21.47945708862596
23	22.153339032972394	28.024151341983867	27.64487265925312	22.17763696579062
24	23.20091364315207	26.921399078826823	28.254816079115475	21.622871198905635
25	22.66625165981822	27.447783185696323	27.864212436650792	22.02175271783467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	161.0
1	161.0
2	297.0
3	433.0
4	433.0
5	433.0
6	804.0
7	1175.0
8	1175.0
9	1175.0
10	1860.0
11	2545.0
12	2545.0
13	2545.0
14	4903.0
15	7261.0
16	7261.0
17	7261.0
18	14226.0
19	21191.0
20	21191.0
21	21191.0
22	35206.5
23	49222.0
24	49222.0
25	49222.0
26	72631.0
27	96040.0
28	96040.0
29	96040.0
30	125212.5
31	154385.0
32	154385.0
33	154385.0
34	176034.5
35	197684.0
36	197684.0
37	197684.0
38	213375.0
39	229066.0
40	229066.0
41	229066.0
42	240051.5
43	251037.0
44	251037.0
45	251037.0
46	256134.0
47	261231.0
48	261231.0
49	261231.0
50	250000.0
51	238769.0
52	238769.0
53	238769.0
54	212158.5
55	185548.0
56	185548.0
57	185548.0
58	159087.0
59	132626.0
60	132626.0
61	132626.0
62	108460.5
63	84295.0
64	84295.0
65	84295.0
66	65031.5
67	45768.0
68	45768.0
69	45768.0
70	33397.0
71	21026.0
72	21026.0
73	21026.0
74	14657.0
75	8288.0
76	8288.0
77	8288.0
78	5730.0
79	3172.0
80	3172.0
81	3172.0
82	2108.0
83	1044.0
84	1044.0
85	1044.0
86	679.0
87	314.0
88	314.0
89	314.0
90	191.0
91	68.0
92	68.0
93	68.0
94	44.5
95	21.0
96	21.0
97	21.0
98	18.5
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3571095159271346
2	0.04005247979056267
3	0.03633834006061075
4	0.03538470958940687
5	0.04246165150728825
6	0.016964584171942586
7	0.016563055552488323
8	0.023439233160642566
9	0.020327386359872034
10	0.020076430972713118
11	0.019223182656372812
12	0.01972509343069064
13	0.019474138043531725
14	0.02750471043261697
15	0.029060633833002242
16	0.021030061443916993
17	0.02298751346375652
18	0.08391948146594083
19	0.025396685180482097
20	0.02283694023146117
21	0.02619974241939062
22	0.019323564811236375
23	0.022435411612006913
24	0.01811897895287359
25	0.02263617592173404
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1992386.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.27816293356348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.60216309758482	60.426107659655834
2	11.251209744483916	16.26433542223011
3	2.8352381293985807	6.147774104163671
4	0.9708815669714216	2.8069414434701527
5	0.44401529830200726	1.6046305037833637
6	0.2335032136611779	1.012630999350798
7	0.14835909301342412	0.7506185888249978
8	0.0926046183717774	0.5354633356060634
9	0.07142193023997671	0.46460213198231776
>10	0.31370320526831286	3.943403415708148
>50	0.01881261699638642	0.9630783231283284
>100	0.014610790408108186	2.1886790568012673
>500	0.0022946188980608596	1.1230614663662664
>1k	0.0011820764020313015	1.7686735489286831
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	3995	0.20051335433997228	No Hit
GTCCTACAGTGGACATTTCTAAATT	3898	0.19564481982908938	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3470	0.1741630386882863	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3348	0.16803972724160882	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2491	0.12502597388257095	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2111	0.10595336445849347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.0038215486356559E-4
10	0.0	0.0	0.0	0.0	1.0038215486356559E-4
11	0.0	0.0	0.0	0.0	1.0038215486356559E-4
12	0.0	0.0	0.0	0.0	1.0038215486356559E-4
13	0.0	0.0	0.0	0.0	1.0038215486356559E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGA	100	1.0149961E-8	13.293236	19
TAGGACC	790	0.0	12.864096	4
TCCAACG	165	0.0	12.668492	18
CAGGACG	75	1.4826195E-5	12.66372	4
AGGACCT	1525	0.0	11.023389	5
GGACCTG	1415	0.0	11.004724	6
GGCGAGG	1000	0.0	10.919443	19
TGGCGAG	2135	0.0	10.814204	18
AGGACGT	2125	0.0	10.726647	5
TATGTCG	125	2.236302E-7	10.641533	16
GGACGTG	2110	0.0	10.574922	6
TCGAACT	135	5.7316356E-8	10.550186	19
TTAGGAC	1380	0.0	10.530159	3
TGTAGGA	2480	0.0	10.4935255	2
GACGTGA	1200	0.0	10.365298	7
CTAGGAC	230	0.0	10.323686	3
TTTAGGA	1500	0.0	10.320932	2
GTCCTAC	2070	0.0	10.313539	1
CTGTAGG	2470	0.0	10.302541	1
GTAGGAC	2380	0.0	10.295924	3
>>END_MODULE