FastQCFastQC Report
Thu 26 May 2016
SRR1513006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992386
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT36750.18445220956180178No Hit
CTTTAGGACGTGAAATATGGCGAGG36040.1808886430641452No Hit
GTCCTAAAGTGTGTATTTCTCATTT36030.18083845198671342No Hit
CTGTAGGACGTGGAATATGGCAAGA34990.175618579933808No Hit
GTATCAACGCAGAGTACTTTTTTTT25970.13034622809033994No Hit
GTCCTACAGTGTGCATTTCTCATTT24240.1216631716946415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.00522431714.2727055
TAGGACC7050.012.4169174
TCCAACG2200.011.21524518
TCTATAC2050.011.130433
AGTGCGA600.005870798611.08481410
CCAACGA1553.1832315E-1011.02043919
GGCGAGG9650.010.62073519
TTATACT3000.010.466654
AGGACGT21700.010.3921085
GGACGTG20650.010.3649746
TGTAGGA22750.010.3218882
GTAGGAC22150.010.1296123
CTAATAC1803.8926373E-1010.0354213
TTAGGAC13450.09.96673
TATGTCG1151.03759485E-59.90416316
GACGTGA11550.09.8754197
AGGACCT14850.09.867555
GGACCTG14850.09.8650676
CCGTTTC1951.6552804E-109.73486213
ACTGGTC2151.0913936E-119.7254068
Produced by FastQC (version 0.11.5)