##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513006_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1992386 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.667091115878147 31.0 31.0 34.0 27.0 34.0 2 30.79232437891051 31.0 31.0 34.0 27.0 34.0 3 30.903021302097084 31.0 31.0 34.0 27.0 34.0 4 34.413442977415016 37.0 35.0 37.0 31.0 37.0 5 34.11447079029867 37.0 35.0 37.0 30.0 37.0 6 34.002564764056764 37.0 35.0 37.0 30.0 37.0 7 33.88675286816912 37.0 35.0 37.0 29.0 37.0 8 33.77456225851818 37.0 35.0 37.0 29.0 37.0 9 35.25320997035715 39.0 35.0 39.0 29.0 39.0 10 35.13921298382944 39.0 35.0 39.0 28.0 39.0 11 35.19110704451848 39.0 35.0 39.0 29.0 39.0 12 35.04377916728987 39.0 35.0 39.0 27.0 39.0 13 35.04991452459513 39.0 35.0 39.0 27.0 39.0 14 36.12251491427866 40.0 36.0 41.0 27.0 41.0 15 36.141277342844205 40.0 36.0 41.0 27.0 41.0 16 36.12082247114766 40.0 36.0 41.0 27.0 41.0 17 36.087126691313834 40.0 36.0 41.0 27.0 41.0 18 36.0070257470189 40.0 36.0 41.0 27.0 41.0 19 35.98137559689739 40.0 36.0 41.0 27.0 41.0 20 35.966481896580284 40.0 36.0 41.0 27.0 41.0 21 35.907756328341996 40.0 36.0 41.0 27.0 41.0 22 35.88706355093842 40.0 36.0 41.0 27.0 41.0 23 35.78016057129492 40.0 36.0 41.0 27.0 41.0 24 35.768802330472106 40.0 36.0 41.0 27.0 41.0 25 35.66908520738451 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7357601940772831 1101 2 0.8050958530928121 1101 3 0.7551404575411453 1101 4 0.6138342530408138 1101 5 0.7763970148362631 1101 6 0.851846466455477 1101 7 0.8937698847527713 1101 8 0.7655335142905173 1101 9 0.6697854968554893 1101 10 0.9750395798880191 1101 11 1.0529942906487761 1101 12 1.0685735489089296 1101 13 1.1207697334304783 1101 14 1.2465290427796916 1101 15 1.0638931086252583 1101 16 1.3171152191570172 1101 17 1.2308843824584699 1101 18 0.7700095674207006 1101 19 1.2010977377914358 1101 20 1.088417970306736 1101 21 1.2562242268766681 1101 22 1.1755441855942692 1101 23 1.1160492940729156 1101 24 0.992948546932972 1101 25 1.0587101443968763 1102 1 0.7154066692765895 1102 2 0.6165152041888042 1102 3 0.6161381940419055 1102 4 0.6817838944181318 1102 5 0.8386563351498992 1102 6 0.7225318154188827 1102 7 0.7569906660270789 1102 8 0.7654729352680079 1102 9 0.9273419341723752 1102 10 1.0728547820397054 1102 11 0.9755517110913203 1102 12 1.009734787164689 1102 13 1.145101451836588 1102 14 1.1343439831067883 1102 15 1.1126338606984234 1102 16 1.1126164566632397 1102 17 1.0693785522409343 1102 18 1.139844397175679 1102 19 1.122541633659182 1102 20 1.1138727621844282 1102 21 1.2424513294179604 1102 22 1.2089463291675813 1102 23 1.2170154268056592 1102 24 1.2532311429391711 1102 25 1.247246539811556 1103 1 0.5225317460374903 1103 2 0.44349910432054074 1103 3 0.516538619343887 1103 4 0.46250094134446584 1103 5 0.5438595845394758 1103 6 0.6773429845909931 1103 7 0.6488146834578004 1103 8 0.6952232804690723 1103 9 0.7675888693855768 1103 10 0.8414571845518708 1103 11 0.8001001746244896 1103 12 0.8763815260602712 1103 13 0.8743570782273551 1103 14 0.987256714930659 1103 15 0.5441595681177773 1103 16 0.7062044980976694 1103 17 0.9048474805774731 1103 18 0.9899073123733615 1103 19 1.0769443694197136 1103 20 1.0449828011426874 1103 21 0.7956772100424203 1103 22 0.9289165128347676 1103 23 0.9044534581110284 1103 24 0.943372902336705 1103 25 0.9890625094885692 1104 1 0.5269446667211 1104 2 0.5851870999095645 1104 3 0.48386126613265645 1104 4 0.5492162704639512 1104 5 0.5214518791610914 1104 6 0.57049188345907 1104 7 0.7084649797960907 1104 8 0.5120148963471891 1104 9 0.7381147401670844 1104 10 0.7949590005054503 1104 11 0.7494906926540494 1104 12 0.7288647799258001 1104 13 0.7948826381577092 1104 14 0.8208896634432179 1104 15 0.8092402203922973 1104 16 0.8236782574032944 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-2.893242900692634 2216 21 -2.7802502723032134 2216 22 -3.128333425500564 2216 23 -2.1080279282935095 2216 24 -2.4750538720071376 2216 25 -2.1856860356819396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40267.0 3 3.0 4 631.0 5 5483.0 6 6431.0 7 5157.0 8 4213.0 9 3820.0 10 3280.0 11 3172.0 12 2841.0 13 2761.0 14 2790.0 15 2802.0 16 2923.0 17 3177.0 18 3435.0 19 4088.0 20 5175.0 21 6873.0 22 8984.0 23 10723.0 24 13087.0 25 15629.0 26 17866.0 27 19975.0 28 22735.0 29 27865.0 30 35163.0 31 45409.0 32 57540.0 33 74496.0 34 96241.0 35 125808.0 36 172248.0 37 266877.0 38 872418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.3415555587927 19.651413539295845 13.293161534933743 27.71386936697772 2 13.653478793767873 20.71210096838665 40.42128382753141 25.213136410314064 3 16.945210416053918 25.04981464435104 30.20790148093793 27.797073458657106 4 11.482141395824264 17.56204435996648 37.96091153862181 32.99490270558744 5 12.499861872764223 38.32131710095544 36.39376408269953 12.7850569435808 6 31.571484326143988 37.71525212935295 18.081003062490993 12.632260482012073 7 27.409957653712404 31.54055332348567 22.916200141187765 18.13328888161416 8 24.56634943978013 37.688221620850584 20.644408549656355 17.10102038971293 9 26.222468165963818 14.751669912253657 20.107340382902265 38.918521538880256 10 14.41971380751372 29.184418241592923 35.14864593942616 21.247222011467194 11 34.10477940530608 21.9912574241374 25.28862094875369 18.615342221802834 12 23.19157768767911 24.629982777288912 32.17770665370906 20.00073288132292 13 29.00397634471425 21.869644959900256 25.837155583396328 23.289223111989166 14 20.52126861270269 22.2139234184379 27.694647522531003 29.57016044632841 15 22.623376897164963 31.600718584875686 23.162164970342562 22.61373954761679 16 21.974167055717032 27.92854525283254 28.128040240357826 21.969247451092606 17 21.577257830009078 28.789993493401024 27.949156150342603 21.6835925262473 18 21.500592509535114 27.468501129055657 29.987557550719668 21.04334881068956 19 23.464462750184207 26.81817086710448 27.516197099680173 22.201169283031142 20 23.579888680920607 27.469129760662476 27.905913342980522 21.045068215436398 21 22.675347724133502 26.765723876521797 27.170264366708423 23.388664032636278 22 23.14590116458532 28.247982441127036 27.036217434097757 21.569898960189885 23 21.967329623878104 27.945588856777754 27.73573996203547 22.351341557308675 24 23.146418414905547 26.961743356959943 28.22480182053076 21.66703640760375 25 22.593188394522006 27.4164234428447 27.88888414849912 22.101504014134168 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 90.0 1 90.0 2 188.5 3 287.0 4 287.0 5 287.0 6 673.5 7 1060.0 8 1060.0 9 1060.0 10 1712.0 11 2364.0 12 2364.0 13 2364.0 14 4742.5 15 7121.0 16 7121.0 17 7121.0 18 14287.0 19 21453.0 20 21453.0 21 21453.0 22 35684.0 23 49915.0 24 49915.0 25 49915.0 26 73345.5 27 96776.0 28 96776.0 29 96776.0 30 126206.0 31 155636.0 32 155636.0 33 155636.0 34 176871.5 35 198107.0 36 198107.0 37 198107.0 38 213551.5 39 228996.0 40 228996.0 41 228996.0 42 240559.5 43 252123.0 44 252123.0 45 252123.0 46 257233.0 47 262343.0 48 262343.0 49 262343.0 50 251311.0 51 240279.0 52 240279.0 53 240279.0 54 212466.5 55 184654.0 56 184654.0 57 184654.0 58 158265.5 59 131877.0 60 131877.0 61 131877.0 62 107265.5 63 82654.0 64 82654.0 65 82654.0 66 63600.0 67 44546.0 68 44546.0 69 44546.0 70 32254.5 71 19963.0 72 19963.0 73 19963.0 74 13974.0 75 7985.0 76 7985.0 77 7985.0 78 5427.5 79 2870.0 80 2870.0 81 2870.0 82 1917.5 83 965.0 84 965.0 85 965.0 86 608.5 87 252.0 88 252.0 89 252.0 90 158.0 91 64.0 92 64.0 93 64.0 94 34.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08045629712314782 2 0.0 3 0.0 4 0.027354137200321624 5 0.07368050166985715 6 0.0764410109286052 7 0.03573604713142935 8 0.06936406901072383 9 0.04838419864423862 10 0.10976788634330897 11 0.0033628021879294474 12 0.012497578280513916 13 0.005219872052905411 14 0.006575031143563546 15 0.006876177608154243 16 0.017868023565714677 17 0.02820738551666193 18 6.524840066131764E-4 19 0.002710318181316271 20 0.004768152356019366 21 1.0038215486356559E-4 22 1.505732322953484E-4 23 0.0 24 0.0 25 0.0025597449490209227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1992386.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.64995514825534 #Duplication Level Percentage of deduplicated Percentage of total 1 82.23741838159792 58.10069920168202 2 12.079160547646236 17.06784301839564 3 3.1529534941150827 6.682680688312967 4 1.0798676883890714 3.0517041500295226 5 0.4797691819041874 1.6947835591522995 6 0.25479554252496833 1.080077619081864 7 0.15804814581701815 0.7816266089266076 8 0.09978316919303756 0.5639741142431103 9 0.07737908147208349 0.49201457718743047 >10 0.34095091845062125 4.138133061897035 >50 0.01996916155149746 0.9861854629928708 >100 0.016352033571246025 2.3334810925031424 >500 0.00227369841099363 1.1485021399492523 >1k 0.0012789553561838943 1.8782947056462533 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3675 0.18445220956180178 No Hit CTTTAGGACGTGAAATATGGCGAGG 3604 0.1808886430641452 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3603 0.18083845198671342 No Hit CTGTAGGACGTGGAATATGGCAAGA 3499 0.175618579933808 No Hit GTATCAACGCAGAGTACTTTTTTTT 2597 0.13034622809033994 No Hit GTCCTACAGTGTGCATTTCTCATTT 2424 0.1216631716946415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 40 0.005224317 14.272705 5 TAGGACC 705 0.0 12.416917 4 TCCAACG 220 0.0 11.215245 18 TCTATAC 205 0.0 11.13043 3 AGTGCGA 60 0.0058707986 11.084814 10 CCAACGA 155 3.1832315E-10 11.020439 19 GGCGAGG 965 0.0 10.620735 19 TTATACT 300 0.0 10.46665 4 AGGACGT 2170 0.0 10.392108 5 GGACGTG 2065 0.0 10.364974 6 TGTAGGA 2275 0.0 10.321888 2 GTAGGAC 2215 0.0 10.129612 3 CTAATAC 180 3.8926373E-10 10.035421 3 TTAGGAC 1345 0.0 9.9667 3 TATGTCG 115 1.03759485E-5 9.904163 16 GACGTGA 1155 0.0 9.875419 7 AGGACCT 1485 0.0 9.86755 5 GGACCTG 1485 0.0 9.865067 6 CCGTTTC 195 1.6552804E-10 9.734862 13 ACTGGTC 215 1.0913936E-11 9.725406 8 >>END_MODULE