FastQCFastQC Report
Thu 26 May 2016
SRR1513005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913312
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30340.15857319663494504No Hit
GTCCTACAGTGGACATTTCTAAATT21070.11012317907377364No Hit
CTGTAGGACGTGGAATATGGCAAGA19780.1033809436202773No Hit
GTCCTAAAGTGTGTATTTCTCATTT19500.10191751266913081No Hit
GGTATCAACGCAGAGTACTTTTTTT19360.10118579719355755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG600.005892835611.07925
TAGACTG1803.6379788E-1110.551625
AAGTCGT909.573111E-510.5494137
GGCGAGG7100.010.43109119
AGGACGT12000.010.3669665
GTCCTAA7000.010.34343051
CTAGACT1502.4590918E-810.1295554
GGACGTG12000.010.1274376
TTAGGAC8450.010.114573
CTGCGCC856.623436E-410.0540229
TAGGACC3500.010.0391124
ACCGTGC951.6530858E-49.9947048
CAAGTCG951.653958E-49.9941826
TAGGACG13100.09.931414
AAGGCGT1252.5893496E-69.874256
GTACTAA1451.55198E-79.8553551
CGTGAAA7450.09.8141049
AATGTCC4100.09.7265538
GACGTGA7150.09.6936567
CTACACT2750.09.669124
Produced by FastQC (version 0.11.5)