##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513005_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1913312 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.075762342994764 31.0 30.0 34.0 23.0 34.0 2 29.09002034169022 31.0 30.0 34.0 22.0 34.0 3 29.31855546821428 31.0 30.0 34.0 23.0 34.0 4 32.76329579284508 35.0 33.0 37.0 25.0 37.0 5 32.88795240922547 35.0 35.0 37.0 26.0 37.0 6 32.87438692696225 37.0 35.0 37.0 26.0 37.0 7 32.75864887692128 37.0 35.0 37.0 25.0 37.0 8 32.73440871117727 37.0 35.0 37.0 25.0 37.0 9 34.05607135689318 39.0 35.0 39.0 25.0 39.0 10 33.94362445853055 39.0 34.0 39.0 23.0 39.0 11 33.99502067618872 39.0 35.0 39.0 25.0 39.0 12 33.895242385977824 39.0 34.0 39.0 23.0 39.0 13 33.88552886303959 39.0 34.0 39.0 23.0 39.0 14 34.891143211352876 39.0 35.0 41.0 22.0 41.0 15 34.82175306484253 39.0 34.0 41.0 22.0 41.0 16 34.706574777140375 39.0 34.0 41.0 19.0 41.0 17 34.65529197538091 39.0 34.0 41.0 19.0 41.0 18 34.35146175845863 39.0 34.0 41.0 17.0 41.0 19 34.58330423893228 39.0 34.0 41.0 18.0 41.0 20 34.66310930992959 39.0 34.0 41.0 18.0 41.0 21 34.60437921259052 39.0 34.0 41.0 18.0 41.0 22 34.516451577160446 39.0 34.0 41.0 18.0 41.0 23 34.24083474101453 39.0 34.0 41.0 15.0 41.0 24 34.244137913732835 39.0 34.0 41.0 11.0 41.0 25 34.160534716763394 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3652321610156477 1101 2 0.6218201932308958 1101 3 0.7135104298856554 1101 4 0.6775191076882123 1101 5 0.3612780416564263 1101 6 0.6697460546302665 1101 7 0.6707889825508673 1101 8 0.46548636938865684 1101 9 0.28475591536430045 1101 10 0.5722836912398677 1101 11 0.5991473521404274 1101 12 0.6599904440023323 1101 13 0.5786304239389608 1101 14 0.5836807199629703 1101 15 0.4461769657588377 1101 16 0.9539120792039313 1101 17 0.8600115516767488 1101 18 0.9508456752447856 1101 19 0.49921461859454297 1101 20 0.8137831728242233 1101 21 0.7702949373128476 1101 22 0.7481622542706035 1101 23 0.9986899287408022 1101 24 0.6775480357717498 1101 25 0.8740228048746559 1102 1 0.8096678269996538 1102 2 0.7661962496518164 1102 3 0.7739117719366888 1102 4 0.9626449530253254 1102 5 0.9424757473180065 1102 6 0.8255786889309746 1102 7 1.0008660032360481 1102 8 1.040818269619244 1102 9 1.0621649214693036 1102 10 1.102833649572716 1102 11 1.0587604418783556 1102 12 1.179269431642794 1102 13 1.075757920847991 1102 14 1.0935874034018411 1102 15 1.088830917245403 1102 16 1.2545706469393352 1102 17 1.1319749734351277 1102 18 1.4436699708477647 1102 19 1.5382113642284239 1102 20 1.4049227139336438 1102 21 1.2822304756275074 1102 22 1.5060897193023735 1102 23 1.6881325781809267 1102 24 1.5650164934275494 1102 25 1.4973408613968004 1103 1 0.4737004607951647 1103 2 0.5817074751484945 1103 3 0.5530778875859212 1103 4 0.4969901239085459 1103 5 0.3043110931365476 1103 6 0.6408247478847571 1103 7 0.4384094488413055 1103 8 0.5116272310276742 1103 9 0.7178953332234315 1103 10 0.662967453559709 1103 11 0.7471382126483164 1103 12 0.8341412734651854 1103 13 0.8422214740118434 1103 14 0.9950332731447062 1103 15 1.0223741458345614 1103 16 0.988054196101956 1103 17 0.8699477040062575 1103 18 1.1338883093860943 1103 19 1.0825962422755495 1103 20 0.9436143103243921 1103 21 0.9046563764986217 1103 22 0.897141196198632 1103 23 1.1894485773535521 1103 24 0.9460696318532982 1103 25 0.8522289794264282 1104 1 0.6288802202531194 1104 2 0.639598426923726 1104 3 0.6242344680949685 1104 4 0.9012550368853169 1104 5 0.660157000465027 1104 6 0.6275525348672844 1104 7 0.8298876829591322 1104 8 0.8990582876178905 1104 9 0.9591439404487758 1104 10 1.0290547600257582 1104 11 0.9396903661347835 1104 12 0.9080121681501367 1104 13 0.9549818724972354 1104 14 1.1399669790576752 1104 15 1.2915168437220856 1104 16 1.2015061146543644 1104 17 1.2240815840621764 1104 18 1.4332255104184668 1104 19 1.2802059202643505 1104 20 1.294650284920408 1104 21 1.3225154512591928 1104 22 1.2064762913954539 1104 23 1.6158289639077594 1104 24 1.3885669825305484 1104 25 1.143063145728405 1105 1 0.7283471156935128 1105 2 0.7562197797958561 1105 3 0.7760125903233224 1105 4 0.8722580783330685 1105 5 0.6373820324339974 1105 6 0.5592399104311028 1105 7 0.7489922057242211 1105 8 0.8128764180847767 1105 9 0.8429446728744949 1105 10 1.0256106511632481 1105 11 0.9352848717697526 1105 12 0.9432217400310634 1105 13 0.8414399226774947 1105 14 0.9304021310268027 1105 15 1.0577218883167774 1105 16 1.0415932778917494 1105 17 0.9908443483469043 1105 18 1.0591949753770677 1105 19 1.0937023795369143 1105 20 1.0427812374143244 1105 21 1.0488250068974807 1105 22 0.6868301241275532 1105 23 0.889896505369741 1105 24 1.061367598299519 1105 25 1.094784111735521 1106 1 0.6335596582812641 1106 2 0.723375820433592 1106 3 0.75411225226242 1106 4 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1107 17 1.3546022626455851 1107 18 1.6328708834955705 1107 19 1.5002698968781374 1107 20 1.263092082974758 1107 21 1.2311391154179105 1107 22 1.3125756348564224 1107 23 1.4096158043735656 1107 24 1.3669059866998055 1107 25 1.4151171065277524 1108 1 0.47475607326721203 1108 2 0.5129879528277606 1108 3 0.4212676727394893 1108 4 0.5999062161231947 1108 5 0.5138993248284294 1108 6 0.5286226422870968 1108 7 0.7376447449385566 1108 8 0.7581771260126047 1108 9 0.8228357033112133 1108 10 0.858175765170067 1108 11 0.7854338735150179 1108 12 0.8071971706116585 1108 13 0.8520223288074789 1108 14 1.0551685376298607 1108 15 0.977996162201137 1108 16 1.0415469471105894 1108 17 1.111193055289526 1108 18 1.1298792549741066 1108 19 1.160326151350759 1108 20 1.1122717670011966 1108 21 0.965545807519419 1108 22 0.8946873689000086 1108 23 1.0799262731678994 1108 24 1.06610017366706 1108 25 1.1604151895989006 1109 1 0.11788412577318041 1109 2 0.21219206642197008 1109 3 0.4585261216353054 1109 4 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2216 25 -4.617926265194864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91721.0 3 3.0 4 739.0 5 4352.0 6 5585.0 7 5138.0 8 4583.0 9 4234.0 10 3876.0 11 3792.0 12 3566.0 13 3543.0 14 3466.0 15 3687.0 16 3599.0 17 3869.0 18 4483.0 19 5422.0 20 6989.0 21 8706.0 22 11030.0 23 13347.0 24 15386.0 25 17531.0 26 19688.0 27 21912.0 28 24778.0 29 29657.0 30 36165.0 31 45074.0 32 56851.0 33 72399.0 34 94539.0 35 124007.0 36 169203.0 37 260712.0 38 729680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.326988349812986 18.47024877615601 12.43660480264024 25.766158071390766 2 15.193752647072664 20.335844893306632 40.23069401670074 24.239708442919962 3 18.460322207558892 24.699198350270528 29.720489230119274 27.11999021205131 4 12.59198375376327 16.83419029722373 38.00914582817011 32.56468012084289 5 13.49865226291723 37.54683092239596 35.592651368783216 13.361865445903584 6 31.986510865172953 36.784971042062324 18.115818159102158 13.112699933662563 7 27.894987487930962 31.520952161396544 22.52036511090445 18.063695239768045 8 25.20095899892724 36.09456783359508 20.834387618750878 17.87008554872681 9 26.52227781001521 15.180067855443857 20.123634918892357 38.17401941564857 10 15.176381410423964 28.583511945214074 34.428511683830834 21.81159496053113 11 34.11557630802784 22.530709627908777 24.5629451968145 18.790768867248882 12 23.13187370419665 24.666711275933057 31.529411272691917 20.672003747178376 13 28.6895135573692 21.855154560491147 25.721362449213448 23.733969432926205 14 21.335183368918983 21.68206504122847 27.478570188774732 29.504181401077815 15 23.358499582014765 30.00370671081957 23.335652859473836 23.302140847691827 16 22.816638402071018 27.764894326657867 26.81883195774321 22.5996353135279 17 22.19183123802832 28.13982083260144 27.367673751390598 22.30067417797964 18 22.115331288645283 27.340325454901233 28.70589244773436 21.83845080871912 19 23.628819262193097 26.94855301431825 27.289100190087435 22.133527533401224 20 23.723347417000983 27.12287503384994 27.423682100149826 21.730095448999247 21 23.39348795191116 26.695204342550394 26.90730256750208 23.00400513803637 22 23.51493119376363 27.387935018307207 26.87008470872619 22.227049079202974 23 22.774239451544624 27.294165865511538 27.16974503662834 22.761849646315497 24 23.177887754445926 26.73619696685348 27.58463321468469 22.5012820640159 25 23.07921928217246 26.94553429298091 27.254810218234 22.72043620661263 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 309.0 1 309.0 2 493.0 3 677.0 4 677.0 5 677.0 6 1195.0 7 1713.0 8 1713.0 9 1713.0 10 2587.5 11 3462.0 12 3462.0 13 3462.0 14 5850.0 15 8238.0 16 8238.0 17 8238.0 18 14870.5 19 21503.0 20 21503.0 21 21503.0 22 33893.5 23 46284.0 24 46284.0 25 46284.0 26 65501.5 27 84719.0 28 84719.0 29 84719.0 30 107957.0 31 131195.0 32 131195.0 33 131195.0 34 151468.0 35 171741.0 36 171741.0 37 171741.0 38 189003.0 39 206265.0 40 206265.0 41 206265.0 42 219088.0 43 231911.0 44 231911.0 45 231911.0 46 240063.5 47 248216.0 48 248216.0 49 248216.0 50 242391.5 51 236567.0 52 236567.0 53 236567.0 54 215339.0 55 194111.0 56 194111.0 57 194111.0 58 168568.5 59 143026.0 60 143026.0 61 143026.0 62 117907.0 63 92788.0 64 92788.0 65 92788.0 66 72190.5 67 51593.0 68 51593.0 69 51593.0 70 37944.0 71 24295.0 72 24295.0 73 24295.0 74 16879.5 75 9464.0 76 9464.0 77 9464.0 78 6546.0 79 3628.0 80 3628.0 81 3628.0 82 2383.5 83 1139.0 84 1139.0 85 1139.0 86 739.0 87 339.0 88 339.0 89 339.0 90 214.0 91 89.0 92 89.0 93 89.0 94 61.0 95 33.0 96 33.0 97 33.0 98 20.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3563977019952836 2 0.04296215149437206 3 0.03951263568095533 4 0.03898998176983158 5 0.044268786272181435 6 0.01907686775601679 7 0.018763275409342542 8 0.025296449298389388 9 0.022055995049422156 10 0.021533341138298405 11 0.02059256409827566 12 0.02121974879162416 13 0.021115218009399406 14 0.02905955745848037 15 0.029529945978491744 16 0.0227877105249954 17 0.024251141476141894 18 0.1054715592647723 19 0.026550818685086384 20 0.023937549129467647 21 0.027125737987322505 22 0.021115218009399406 23 0.023467160609456274 24 0.01991311401381479 25 0.02388528373835527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1913312.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.64875085857962 #Duplication Level Percentage of deduplicated Percentage of total 1 81.33236337134935 58.27362239933276 2 12.546183515217077 17.978367538156142 3 3.344946040157457 7.189836163999025 4 1.2158038248647907 3.484433013625823 5 0.576579668614545 2.0655606513342963 6 0.290492444549919 1.2488052471514144 7 0.16728163705425103 0.8389864235560762 8 0.11114671976061578 0.6370818906301383 9 0.06692266878166152 0.431543306009566 >10 0.31945588763785 3.905950355405366 >50 0.016307050798503763 0.8315151329963504 >100 0.010687989158520279 1.6037687697281269 >500 0.0010975092332460355 0.5608260011864058 >1k 7.316728221640219E-4 0.9497031068884751 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3034 0.15857319663494504 No Hit GTCCTACAGTGGACATTTCTAAATT 2107 0.11012317907377364 No Hit CTGTAGGACGTGGAATATGGCAAGA 1978 0.1033809436202773 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1950 0.10191751266913081 No Hit GGTATCAACGCAGAGTACTTTTTTT 1936 0.10118579719355755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.0453078222474955E-4 0.0 12 0.0 0.0 0.0 1.0453078222474955E-4 5.2265391112374775E-5 13 0.0 0.0 0.0 1.567961733371243E-4 5.2265391112374775E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 60 0.0058928356 11.0792 5 TAGACTG 180 3.6379788E-11 10.55162 5 AAGTCGT 90 9.573111E-5 10.549413 7 GGCGAGG 710 0.0 10.431091 19 AGGACGT 1200 0.0 10.366966 5 GTCCTAA 700 0.0 10.3434305 1 CTAGACT 150 2.4590918E-8 10.129555 4 GGACGTG 1200 0.0 10.127437 6 TTAGGAC 845 0.0 10.11457 3 CTGCGCC 85 6.623436E-4 10.054022 9 TAGGACC 350 0.0 10.039112 4 ACCGTGC 95 1.6530858E-4 9.994704 8 CAAGTCG 95 1.653958E-4 9.994182 6 TAGGACG 1310 0.0 9.93141 4 AAGGCGT 125 2.5893496E-6 9.87425 6 GTACTAA 145 1.55198E-7 9.855355 1 CGTGAAA 745 0.0 9.814104 9 AATGTCC 410 0.0 9.726553 8 GACGTGA 715 0.0 9.693656 7 CTACACT 275 0.0 9.66912 4 >>END_MODULE