FastQCFastQC Report
Thu 26 May 2016
SRR1513005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913312
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37640.19672693214697864No Hit
TATCAACGCAGAGTACTTTTTTTTT27740.14498419494572762No Hit
GGTATCAACGCAGAGTACTTTTTTT23490.12277140372296835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1001.9541385E-611.38830218
CTAACGT600.0058107411.1001014
AAGGCCG1158.6486216E-710.7553765
GGACCGT750.002620736610.1460056
GCCGTCC750.002639017810.13750658
GTCCTAC10150.010.1173041
TGGACCG951.6193782E-410.0151285
TAAACTG2700.09.86675555
CTAGCAC1951.6189006E-109.7511763
TAGGACC4100.09.5143724
GTCTACC1304.1912535E-69.5083921
GGTCTAA900.00110480089.5083911
GGCCGCA800.004545839.49248511
GTGCGCC1201.7164895E-59.49248511
TGGCGAG12200.09.49025218
GGCGAGG5700.09.49025219
GGTATCA10800.09.4203521
AGGACCT8500.09.4024385
TAGAACT3450.09.3764824
CTAGGAC2600.09.1417273
Produced by FastQC (version 0.11.5)