FastQCFastQC Report
Thu 26 May 2016
SRR1513004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497446
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46700.18699103003628506No Hit
GTCCTAAAGTGTGTATTTCTCATTT43510.17421798108948103No Hit
CTGTAGGACGTGGAATATGGCAAGA40420.1618453412005705No Hit
CTTTAGGACGTGAAATATGGCGAGG39400.15776116881005636No Hit
GTATCAACGCAGAGTACTTTTTTTT33200.13293580722065662No Hit
GTCCTACAGTGTGCATTTCTCATTT31060.12436705338173479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9200.013.2167364
TATACCG604.0966668E-412.6655325
GGCGAGG10400.011.77669919
GCGTGCG1001.944225E-611.3934999
ATACCGT600.005903234211.0767926
TGTAGGA26850.011.0739392
GTCCTAG3250.010.8513751
GGCGTGC1053.4921359E-610.8509528
CTGTAGG27200.010.7931511
GTAGGAC25550.010.7822453
GGTATCA13550.010.7626331
TGGCGAG24550.010.75904618
AGGACCT18100.010.70622355
AGGACGT23600.010.4651645
CTAGGAC2550.010.4308563
TTAGGAC15250.010.4027633
GGACGTG22900.010.3650586
ATGTCGA1106.039367E-610.36499117
ATAGGAC3250.010.2302623
TCGAACT1303.8751932E-710.224730519
Produced by FastQC (version 0.11.5)