##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513004_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2497446
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.294745111606016	31.0	30.0	34.0	25.0	34.0
2	29.31825993434893	31.0	30.0	34.0	23.0	34.0
3	29.550202486860577	31.0	30.0	34.0	25.0	34.0
4	33.00933433595761	35.0	35.0	37.0	26.0	37.0
5	33.14415206575037	37.0	35.0	37.0	27.0	37.0
6	33.144110423208346	37.0	35.0	37.0	27.0	37.0
7	33.03548465111958	37.0	35.0	37.0	26.0	37.0
8	33.017191562900656	37.0	35.0	37.0	26.0	37.0
9	34.364570445166784	39.0	35.0	39.0	26.0	39.0
10	34.24849866623743	39.0	35.0	39.0	25.0	39.0
11	34.304727309419306	39.0	35.0	39.0	26.0	39.0
12	34.20891262513784	39.0	35.0	39.0	25.0	39.0
13	34.20882893964474	39.0	35.0	39.0	25.0	39.0
14	35.222721532317415	39.0	35.0	41.0	25.0	41.0
15	35.16869393772678	40.0	35.0	41.0	25.0	41.0
16	35.057477118624384	39.0	35.0	41.0	24.0	41.0
17	35.0026198764658	39.0	35.0	41.0	24.0	41.0
18	34.7035107065378	39.0	34.0	41.0	19.0	41.0
19	34.937875333440644	39.0	34.0	41.0	23.0	41.0
20	35.0224441289221	40.0	35.0	41.0	24.0	41.0
21	34.96190468182295	39.0	35.0	41.0	24.0	41.0
22	34.8742347181881	39.0	35.0	41.0	22.0	41.0
23	34.60808081536097	39.0	34.0	41.0	18.0	41.0
24	34.60722073670462	39.0	34.0	41.0	18.0	41.0
25	34.52325615849151	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4330940558316456
1101	2	0.6790359429312609
1101	3	0.7831762720817323
1101	4	0.6707817137030005
1101	5	0.5364187746720148
1101	6	0.5594351154580579
1101	7	0.6349142409936661
1101	8	0.41817070042942817
1101	9	0.4163309741944232
1101	10	0.5566865207519882
1101	11	0.7201265728128021
1101	12	0.8874645254851004
1101	13	0.7254353685569797
1101	14	0.585490267786497
1101	15	0.44125491050507293
1101	16	0.9735379651534473
1101	17	0.8910336421775042
1101	18	1.1002461801565104
1101	19	0.6418683310912741
1101	20	0.7487077026556648
1101	21	0.748038273806344
1101	22	0.8701382273138876
1101	23	1.1175546970219088
1101	24	0.8291902970709302
1101	25	1.1311689124340347
1102	1	0.6314662618244
1102	2	0.7203083629031859
1102	3	0.7421372352690163
1102	4	0.8577396900140712
1102	5	0.8067605123862762
1102	6	0.7251335453407393
1102	7	0.9374023677592618
1102	8	0.9217530783950565
1102	9	0.8967567646393633
1102	10	1.0480411734941697
1102	11	0.9988798731638155
1102	12	1.1207265770354766
1102	13	1.0241687563999093
1102	14	0.989910496646516
1102	15	1.1544013142766758
1102	16	1.1318252753456264
1102	17	1.1248855750156181
1102	18	1.4297595147120745
1102	19	1.426084590564102
1102	20	1.3575909829737256
1102	21	1.1148099450519027
1102	22	1.3589378429810708
1102	23	1.5471736160457183
1102	24	1.5235791985240894
1102	25	1.3920084494682214
1103	1	0.6085658525385433
1103	2	0.7169177905236097
1103	3	0.6872916239043931
1103	4	0.7979777486990685
1103	5	0.46635063856913916
1103	6	0.6974683692628929
1103	7	0.6675229323320266
1103	8	0.6405658554306299
1103	9	0.9411014009694441
1103	10	0.864968883747224
1103	11	0.9782565043459783
1103	12	1.051125116280211
1103	13	1.0146072116316205
1103	14	1.253025772840978
1103	15	1.343674301083496
1103	16	1.3657848955231273
1103	17	1.1850489627135232
1103	18	1.4394935279019876
1103	19	1.4306491241317474
1103	20	1.2999979627184857
1103	21	1.1438450691605695
1103	22	1.2607409219343708
1103	23	1.4752539575854513
1103	24	1.3053236774114083
1103	25	1.2222051222649242
1104	1	0.6835846562090175
1104	2	0.7073648857966077
1104	3	0.7371531968834368
1104	4	0.9177281639801294
1104	5	0.6887306871546244
1104	6	0.6653574612136381
1104	7	0.9027925369588488
1104	8	0.9016705830734608
1104	9	0.9273175903696114
1104	10	0.9926190810657403
1104	11	1.0000428370381158
1104	12	0.9810090296200897
1104	13	1.104745446615084
1104	14	1.234655466957264
1104	15	1.2910547497470688
1104	16	1.0884750846480458
1104	17	1.221216022523599
1104	18	1.3838276219171348
1104	19	1.1719951148691408
1104	20	1.2669652747377924
1104	21	1.2963954870021368
1104	22	1.316942932566846
1104	23	1.5155178170908314
1104	24	1.3335045383154522
1104	25	1.189634604499858
1105	1	0.6327297945432733
1105	2	0.7672159537657279
1105	3	0.7746315661570549
1105	4	0.9118526699292175
1105	5	0.5922133620947179
1105	6	0.5224648827286629
1105	7	0.6800521159148403
1105	8	0.7519377033454191
1105	9	0.6849332699583996
1105	10	0.7689088611234922
1105	11	0.827780979816275
1105	12	0.9156477580917581
1105	13	0.8041894473523215
1105	14	0.9625137946589319
1105	15	1.1418239844545326
1105	16	1.0148200294576952
1105	17	0.9461885041438762
1105	18	0.9285397412675493
1105	19	0.9305630583886213
1105	20	1.101753626399649
1105	21	1.1118156350638202
1105	22	0.7675725274962986
1105	23	1.0336732920964593
1105	24	1.0769473564023286
1105	25	1.1795925717747409
1106	1	0.5203384620625329
1106	2	0.5002261392046599
1106	3	0.5691645051662206
1106	4	0.5307663372962352
1106	5	0.5921178260269997
1106	6	0.5214482419442774
1106	7	0.32596005111778936
1106	8	0.5574626188473815
1106	9	0.6061592146616661
1106	10	0.7405100989055313
1106	11	0.6536581227169123
1106	12	0.6741658833011002
1106	13	0.6729586687524005
1106	14	0.8270931314635348
1106	15	0.9482188110432475
1106	16	0.9490957823275821
1106	17	0.8502590638101495
1106	18	1.1610947030531236
1106	19	0.6382114628221061
1106	20	0.7311391505919644
1106	21	0.5057053232584536
1106	22	0.5539354199173587
1106	23	1.0000217589519167
1106	24	0.9327284303647474
1106	25	0.8717407249978493
1107	1	0.5009591130416382
1107	2	0.4950201839992623
1107	3	0.6433103064803163
1107	4	0.7033928101812208
1107	5	0.6331957739108702
1107	6	0.49380513148971517
1107	7	0.7358755700657156
1107	8	0.6376094472640546
1107	9	0.8637859486744262
1107	10	0.6912955339443982
1107	11	0.5875680510435544
1107	12	0.7457592228020218
1107	13	0.8035639007582134
1107	14	0.9644633136561112
1107	15	1.1158081291764574
1107	16	0.9406816237310096
1107	17	1.082325865268487
1107	18	1.3542737969148817
1107	19	1.1157987416717532
1107	20	1.016515311367094
1107	21	1.0346578029775273
1107	22	1.030667505308628
1107	23	1.1117127602401453
1107	24	1.1574090462283877
1107	25	1.1742561937097662
1108	1	0.27881719291725915
1108	2	0.3129372910811412
1108	3	0.2955323045011724
1108	4	0.5171341117742543
1108	5	0.3645192356636642
1108	6	0.508434270483761
1108	7	0.5134995192664249
1108	8	0.43520155766763935
1108	9	0.6098285329416413
1108	10	0.5318039581867069
1108	11	0.3750837423847102
1108	12	0.4518649888865198
1108	13	0.5265637629322129
1108	14	0.7035541132253869
1108	15	0.6201610509884645
1108	16	0.5322831122517897
1108	17	0.5912596423552614
1108	18	0.8706855020108719
1108	19	0.7297739012623552
1108	20	0.6668357568127874
1108	21	0.5309451771245435
1108	22	0.42893493831428486
1108	23	0.6058899836511316
1108	24	0.7247354350484159
1108	25	0.756299474047843
1109	1	-0.08827306652036881
1109	2	-0.010403408255918123
1109	3	0.11794127305985569
1109	4	0.1477568825541482
1109	5	0.07400495755844361
1109	6	0.12068804569277347
1109	7	0.11971435505315497
1109	8	0.26920383404812753
1109	9	0.38639506634388
1109	10	0.4741211106185972
1109	11	-0.028439891707726872
1109	12	0.29198850947697963
1109	13	0.3305417853979975
1109	14	0.09749748271237024
1109	15	0.5186624443996379
1109	16	0.4727854377741991
1109	17	0.3049337141278272
1109	18	-1.4881138499498903
1109	19	-0.05027124589391008
1109	20	0.3017551799183664
1109	21	0.3236357764340241
1109	22	0.47274296144586003
1109	23	0.07056987953367155
1109	24	0.4382897907661629
1109	25	0.5111809152059479
1110	1	0.29100626570084387
1110	2	0.2450518035527942
1110	3	0.332304330384698
1110	4	0.14979667883359582
1110	5	0.2531514670114845
1110	6	0.2847870372002035
1110	7	0.33655618095549045
1110	8	0.29728226393388724
1110	9	0.5142802018205757
1110	10	0.6529281700665805
1110	11	0.4216217173307868
1110	12	0.02876114368989846
1110	13	0.20984057975650217
1110	14	0.45784988641270985
1110	15	0.5282387637808981
1110	16	0.6937665064224632
1110	17	0.28028403259916246
1110	18	0.6671552077793592
1110	19	0.5192257084079799
1110	20	0.5460044974797853
1110	21	0.5435023849670273
1110	22	0.6142261279608761
1110	23	0.41648276751264746
1110	24	0.6053602967817326
1110	25	0.6120536151351743
1111	1	0.13288191623427892
1111	2	0.27883075229076226
1111	3	0.2910279837804808
1111	4	0.09761983131863161
1111	5	0.15060706810437807
1111	6	0.3896001714703061
1111	7	0.41512435685973514
1111	8	0.22498360403276507
1111	9	0.4858722197752954
1111	10	0.422212420652059
1111	11	0.2660507116060984
1111	12	0.26735647936515505
1111	13	0.24666483814838358
1111	14	0.1952254718927975
1111	15	0.3560141020582819
1111	16	0.271220643815127
1111	17	0.26759863056949484
1111	18	0.5647856397335644
1111	19	0.40649232953730774
1111	20	0.19764384174207805
1111	21	0.4056828104158967
1111	22	0.16607364292314486
1111	23	0.49025732514990494
1111	24	0.5506545005288004
1111	25	0.056323228718312635
1112	1	-0.17823290098579392
1112	2	-0.04353282590374974
1112	3	-0.04823165351062286
1112	4	0.12038738848601582
1112	5	-0.02347535235407605
1112	6	0.054857600025236763
1112	7	-0.3500211550619525
1112	8	-0.06904055063062486
1112	9	0.21140014713974864
1112	10	0.23145159314995567
1112	11	0.1628073555266809
1112	12	0.09684318589279428
1112	13	-0.16505269977473347
1112	14	-0.09945301398751383
1112	15	0.14064687184042413
1112	16	0.14899808110232726
1112	17	0.23989651372649234
1112	18	0.22523835338451903
1112	19	-0.053299400234706695
1112	20	-0.21693676357421054
1112	21	-0.021893796362405737
1112	22	-0.076531654769731
1112	23	0.21194281636191192
1112	24	-0.0416010282196666
1112	25	0.05834076634022978
1113	1	-0.2919446645854258
1113	2	-0.09241150823770994
1113	3	-0.2570917551162033
1113	4	-0.0184033684829501
1113	5	-0.13462163787387027
1113	6	0.0333719479221557
1113	7	-0.23651339583221187
1113	8	0.09055327329908636
1113	9	-0.06445005144048821
1113	10	-0.2370664110133447
1113	11	-0.21383039842408635
1113	12	0.09243901150197331
1113	13	0.05648839900524649
1113	14	0.06313846881707974
1113	15	-0.2743766891089905
1113	16	-0.31418392566040154
1113	17	-0.3385029877459331
1113	18	-0.3913245462563424
1113	19	-0.3335498849394938
1113	20	-0.4545565561974172
1113	21	-0.32215329925274716
1113	22	-0.19335085596171808
1113	23	-0.6444478278991852
1113	24	-0.06340195391217662
1113	25	-0.031826313757150615
1114	1	-1.1612850089885676
1114	2	-0.9676868899663766
1114	3	-0.8629452147153813
1114	4	-0.9944913744216706
1114	5	-0.9279357721689081
1114	6	-1.3722982998589757
1114	7	-1.6651280318510224
1114	8	-1.0864017085286832
1114	9	-1.2311254765889075
1114	10	-1.2358582760819345
1114	11	-1.9609411872825788
1114	12	-1.5106056866788435
1114	13	-1.3165650842117103
1114	14	-1.7331835177859887
1114	15	-1.4835961771749524
1114	16	-2.3061676052951796
1114	17	-4.327917163137776
1114	18	-3.3062716998089066
1114	19	-2.151931998999565
1114	20	-1.325555902089448
1114	21	-2.2556945645215407
1114	22	-2.4523470830755514
1114	23	-2.317724481694114
1114	24	-1.958193464446353
1114	25	-1.6882123749581126
1115	1	-1.4141084035374298
1115	2	-0.9878835959328818
1115	3	-1.0848611391550733
1115	4	-0.9937906560072314
1115	5	-0.8847107192969332
1115	6	-0.9664807534356044
1115	7	-2.173769038517772
1115	8	-1.4683164103589235
1115	9	-1.2629724473769102
1115	10	-1.0102011528916535
1115	11	-1.9268211863670004
1115	12	-1.5381158515215887
1115	13	-1.4772894747792762
1115	14	-1.3279516898043724
1115	15	-2.8039764197785573
1115	16	-1.5477566158618288
1115	17	-1.4498968665761467
1115	18	-1.671418326848645
1115	19	-1.6763950794788656
1115	20	-1.361883238620237
1115	21	-2.2363246412810867
1115	22	-2.243249264430716
1115	23	-1.439929897646529
1115	24	-1.3941977084501573
1115	25	-1.8445020401809558
1116	1	-1.384722519402942
1116	2	-2.1996215786205866
1116	3	-1.771633398553032
1116	4	-1.4476844610582589
1116	5	-1.884437382630935
1116	6	-1.675124331193306
1116	7	-1.2734974558838594
1116	8	-1.9804791611489705
1116	9	-2.065076701197043
1116	10	-2.976887698588577
1116	11	-1.5629004569235292
1116	12	-1.540451637576453
1116	13	-2.2221567384015266
1116	14	-1.9818168456647385
1116	15	-1.6509458908501884
1116	16	-1.5743141232653102
1116	17	-1.9861994838843557
1116	18	-1.653417572614849
1116	19	-2.025583401133268
1116	20	-2.340573210526493
1116	21	-2.8211817899850047
1116	22	-2.05100150155247
1116	23	-1.8771925551805353
1116	24	-2.5072143805250917
1116	25	-2.281013387306828
1201	1	0.31815302878794327
1201	2	0.2612124225715782
1201	3	0.44458696729100566
1201	4	0.47995060702395875
1201	5	0.5324171469187107
1201	6	0.4884689625397698
1201	7	0.5196332204917837
1201	8	-0.00321474622929685
1201	9	0.36331178770088
1201	10	0.5679443371225545
1201	11	0.18440145451764778
1201	12	-0.21578681986354553
1201	13	0.06203888997859508
1201	14	0.5707838787872319
1201	15	0.23923448603813569
1201	16	0.6215888021804616
1201	17	0.7190979438371556
1201	18	1.0723397630012244
1201	19	0.5907264381011927
1201	20	0.6679595603250661
1201	21	0.5893586724816018
1201	22	0.7388321863198541
1201	23	0.9484029188892293
1201	24	0.8861475440926299
1201	25	0.82596476301417
1202	1	0.42973182706359836
1202	2	0.5886082301884912
1202	3	0.3495807531489241
1202	4	0.6679502309048289
1202	5	0.5467357198183009
1202	6	0.5062047201270374
1202	7	0.5232939858335399
1202	8	0.6078828252966417
1202	9	0.848805221177912
1202	10	0.9683499180340576
1202	11	0.7195492724992008
1202	12	0.7253530426446062
1202	13	0.6753784717425688
1202	14	0.9515889979109673
1202	15	0.5775455291699814
1202	16	0.855267183929243
1202	17	1.0927840325991625
1202	18	1.3177673835439734
1202	19	1.097737855136593
1202	20	1.060595089814278
1202	21	0.9050066919743855
1202	22	1.0887107388784116
1202	23	1.1546082514460565
1202	24	1.35591077103647
1202	25	1.118871027059292
1203	1	0.20341727884663285
1203	2	0.3146905070471284
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2211	16	0.002362130558154263
2211	17	0.17887432977975948
2211	18	0.1582570004988142
2211	19	-0.028501881590848654
2211	20	-0.06794194469880921
2211	21	-0.11704792174866441
2211	22	-0.2767804397665472
2211	23	0.26567229674487436
2211	24	0.14307363956952912
2211	25	0.10804561059138962
2212	1	0.09100984996257466
2212	2	0.0138839658601988
2212	3	-0.03814791822571095
2212	4	-0.08709833287666413
2212	5	-0.0689953353257522
2212	6	-0.11069626665695864
2212	7	-0.2232699096626618
2212	8	-0.15117981207050946
2212	9	0.0029031785925539566
2212	10	0.0774191798358217
2212	11	-0.21180117241631535
2212	12	0.029524741827486878
2212	13	0.009882763759968327
2212	14	0.01263457504813914
2212	15	-0.1548364275134091
2212	16	0.07797605389313134
2212	17	-0.028836735465517904
2212	18	-2.771124055837827
2212	19	-0.9437296933501642
2212	20	-0.24034142037194073
2212	21	-0.2736790869564203
2212	22	0.013760524842417965
2212	23	-0.08371887854075766
2212	24	-0.4469379669317064
2212	25	-0.17638734129624112
2213	1	-0.08550031915246592
2213	2	0.009511447736091583
2213	3	-0.32418667218599495
2213	4	-0.4108978421196028
2213	5	-0.28012894776796315
2213	6	-0.3118844495178763
2213	7	-0.17776472735641846
2213	8	-0.4412289267463336
2213	9	-0.2991301248207918
2213	10	-0.4337068984852621
2213	11	-0.42451344303155736
2213	12	-0.4473968162807225
2213	13	-0.22878754180059957
2213	14	-0.2902951588804754
2213	15	-0.5448274774971082
2213	16	-1.0321850074307193
2213	17	-0.540152985395693
2213	18	-0.4692122076529657
2213	19	-0.4500079544230502
2213	20	-1.2005067034015937
2213	21	-0.9438018801408958
2213	22	-0.7197253960934802
2213	23	-1.1790474636458015
2213	24	-0.9242357115016162
2213	25	-0.9930815248248379
2214	1	-1.4761401470913924
2214	2	-3.4013977712619052
2214	3	-1.679181429959204
2214	4	-1.2907084312795867
2214	5	-1.273380634052117
2214	6	-1.2559644628893523
2214	7	-1.473548153631647
2214	8	-1.1704479523690097
2214	9	-1.4059798296951698
2214	10	-1.4322774765391912
2214	11	-1.0878733857883702
2214	12	-1.606348868208464
2214	13	-1.6107232012368158
2214	14	-2.838606112026433
2214	15	-2.9674900590486075
2214	16	-2.1047382550558353
2214	17	-2.6607422973309838
2214	18	-2.0021227555105625
2214	19	-4.049427510760754
2214	20	-2.4166307984440607
2214	21	-2.363018819923667
2214	22	-2.5429666255510597
2214	23	-2.380442075548352
2214	24	-2.728421757370757
2214	25	-2.5155706086990826
2215	1	-0.7869997293724609
2215	2	-2.2976773689840684
2215	3	-1.1680318718745824
2215	4	-0.7821558504747017
2215	5	-0.7009066276716069
2215	6	-1.2631077763196075
2215	7	-1.6913598133066543
2215	8	-1.6173770617831345
2215	9	-1.2739941573672482
2215	10	-2.875747080964441
2215	11	-1.8878136389795657
2215	12	-2.0269175526137957
2215	13	-1.4643229970513048
2215	14	-2.805401685733095
2215	15	-2.5566006756258517
2215	16	-2.216435737914395
2215	17	-2.5326530303378973
2215	18	-1.3402911386092953
2215	19	-1.8771750329446846
2215	20	-2.230699567685818
2215	21	-1.7862990902671356
2215	22	-1.3245082146196197
2215	23	-2.0491286776661113
2215	24	-1.7694594614514898
2215	25	-1.7361351885824732
2216	1	-2.119668744572248
2216	2	-2.1062618950653658
2216	3	-2.6239405057485996
2216	4	-3.459349089200046
2216	5	-2.4858076258747275
2216	6	-2.4941119690423363
2216	7	-2.365448546851354
2216	8	-2.879488231972182
2216	9	-3.128730559240484
2216	10	-2.7241129916886777
2216	11	-3.1397313714811617
2216	12	-3.6485113265072115
2216	13	-4.188611366775344
2216	14	-3.5948496273641624
2216	15	-3.33415955722473
2216	16	-2.6014680096275953
2216	17	-2.616645188001222
2216	18	-2.709311849483278
2216	19	-3.084641030498176
2216	20	-2.7674569653207044
2216	21	-3.2652358474277356
2216	22	-3.778178561813686
2216	23	-3.620671185113114
2216	24	-3.650799526805283
2216	25	-3.652542436610595
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105817.0
3	0.0
4	876.0
5	5160.0
6	6470.0
7	5843.0
8	5232.0
9	4828.0
10	4664.0
11	4474.0
12	4397.0
13	4285.0
14	4316.0
15	4505.0
16	4575.0
17	4940.0
18	5342.0
19	6549.0
20	8469.0
21	10928.0
22	13835.0
23	16902.0
24	19357.0
25	22321.0
26	24708.0
27	28045.0
28	31653.0
29	37932.0
30	46562.0
31	58697.0
32	74005.0
33	94865.0
34	123567.0
35	162633.0
36	223534.0
37	345334.0
38	971826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7556341005129	18.84516410056112	12.690368384422381	25.7088334145036
2	14.60365936705352	20.305857903498975	41.581396802099675	23.50908592734783
3	18.325667879840513	24.902202379187994	29.937127525854386	26.835002215117115
4	12.210770193963471	17.006041655901598	38.706373440267	32.07681470986793
5	13.001192141480274	37.96642134830561	36.111872222943276	12.920514287270846
6	31.318241671632606	37.38618378475359	18.46902372759719	12.826550816016608
7	27.672694783103545	31.495169644147968	22.86941119037445	17.962724382374038
8	24.52248023517538	37.32952588451094	20.99232636191056	17.155667518403114
9	26.3749840805018	15.18864305000765	20.438713336094047	37.99765953339651
10	14.782372232970904	28.858241684745412	35.08712840120355	21.272257681080134
11	33.8144608170984	22.2801766292268	25.437948691660573	18.467413862014222
12	23.178628655508255	24.705410718912784	32.14840819863906	19.967552426939896
13	28.798310264605863	22.172130179149917	25.88560725266214	23.143952303582076
14	20.652901376702555	22.3474154335033	27.824226808872126	29.175456380922014
15	22.623763288317335	31.37128305607868	23.348307514352705	22.65664614125128
16	21.982384712057485	28.017655337251224	28.12771083758323	21.872249113108058
17	21.541529362197043	28.76301021593314	28.002382169135608	21.693078252734207
18	21.47894084340841	27.468912998080448	30.030346477327523	21.02179968118362
19	23.36815342950312	27.03151270769302	27.557578917941683	22.04275494486217
20	23.49128678166812	27.459413607935613	28.10213160660276	20.94716800379351
21	22.788813218818937	26.874956944958434	27.177543771958074	23.158686064264554
22	23.15133350046977	28.22209549043998	27.219116670578085	21.40745433851216
23	22.184050337759768	27.97484714002562	27.76134179558355	22.079760726631058
24	23.021961320490625	26.95723355524131	28.418878637843058	21.601926486425008
25	22.609382765889023	27.5067814170949	27.919693924098592	21.96414189291749
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	263.0
1	263.0
2	465.5
3	668.0
4	668.0
5	668.0
6	1243.0
7	1818.0
8	1818.0
9	1818.0
10	2886.0
11	3954.0
12	3954.0
13	3954.0
14	7322.0
15	10690.0
16	10690.0
17	10690.0
18	19744.0
19	28798.0
20	28798.0
21	28798.0
22	46608.0
23	64418.0
24	64418.0
25	64418.0
26	92972.0
27	121526.0
28	121526.0
29	121526.0
30	157365.0
31	193204.0
32	193204.0
33	193204.0
34	219839.0
35	246474.0
36	246474.0
37	246474.0
38	267041.5
39	287609.0
40	287609.0
41	287609.0
42	301072.5
43	314536.0
44	314536.0
45	314536.0
46	319870.0
47	325204.0
48	325204.0
49	325204.0
50	311897.5
51	298591.0
52	298591.0
53	298591.0
54	265528.5
55	232466.0
56	232466.0
57	232466.0
58	198871.0
59	165276.0
60	165276.0
61	165276.0
62	134796.0
63	104316.0
64	104316.0
65	104316.0
66	80447.5
67	56579.0
68	56579.0
69	56579.0
70	41148.5
71	25718.0
72	25718.0
73	25718.0
74	17908.5
75	10099.0
76	10099.0
77	10099.0
78	6865.0
79	3631.0
80	3631.0
81	3631.0
82	2407.0
83	1183.0
84	1183.0
85	1183.0
86	750.5
87	318.0
88	318.0
89	318.0
90	197.0
91	76.0
92	76.0
93	76.0
94	49.5
95	23.0
96	23.0
97	23.0
98	15.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3543620162357865
2	0.042363278325136956
3	0.03859943318093764
4	0.03783865597093991
5	0.043964914556711136
6	0.01753791673573723
7	0.017017384960475623
8	0.02346397079256168
9	0.020340780140992037
10	0.01998041198888785
11	0.018578980286260444
12	0.01982024836573043
13	0.019499921119415595
14	0.02734793865412906
15	0.0281887976757055
16	0.02098143463362171
17	0.022502989053617174
18	0.10382606871179598
19	0.024505034343084892
20	0.022022498184144923
21	0.025706261516765527
22	0.01937979840204753
23	0.022142620901512984
24	0.018619021192049796
25	0.021982457278355568
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2497446.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.77889071686843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.38466921331087	59.85259058924465
2	11.33875258751242	16.277661656893265
3	2.8449234664748397	6.126163517938562
4	0.9885331461791583	2.838232506783837
5	0.459120045151758	1.6477563773435873
6	0.2535240937969303	1.0918606933645778
7	0.14923193576054924	0.7498191965896236
8	0.11156751494452652	0.6406553970204604
9	0.08131786313399755	0.5253215410102028
>10	0.3509754534756155	4.333925332623057
>50	0.020627490195225296	1.0249259382639937
>100	0.013680580645546885	2.055036029597058
>500	0.001957842357292205	1.0424243888897022
>1k	0.001118767061294873	1.7936268344374395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4670	0.18699103003628506	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4351	0.17421798108948103	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4042	0.1618453412005705	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3940	0.15776116881005636	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3320	0.13293580722065662	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3106	0.12436705338173479	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.0040905789354407E-5	0.0	0.0	0.0	0.0
8	4.0040905789354407E-5	0.0	0.0	0.0	0.0
9	4.0040905789354407E-5	0.0	0.0	0.0	0.0
10	4.0040905789354407E-5	0.0	0.0	0.0	0.0
11	4.0040905789354407E-5	0.0	0.0	0.0	0.0
12	4.0040905789354407E-5	0.0	0.0	0.0	8.008181157870881E-5
13	4.0040905789354407E-5	0.0	0.0	0.0	8.008181157870881E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	920	0.0	13.216736	4
TATACCG	60	4.0966668E-4	12.665532	5
GGCGAGG	1040	0.0	11.776699	19
GCGTGCG	100	1.944225E-6	11.393499	9
ATACCGT	60	0.0059032342	11.076792	6
TGTAGGA	2685	0.0	11.073939	2
GTCCTAG	325	0.0	10.851375	1
GGCGTGC	105	3.4921359E-6	10.850952	8
CTGTAGG	2720	0.0	10.793151	1
GTAGGAC	2555	0.0	10.782245	3
GGTATCA	1355	0.0	10.762633	1
TGGCGAG	2455	0.0	10.759046	18
AGGACCT	1810	0.0	10.7062235	5
AGGACGT	2360	0.0	10.465164	5
CTAGGAC	255	0.0	10.430856	3
TTAGGAC	1525	0.0	10.402763	3
GGACGTG	2290	0.0	10.365058	6
ATGTCGA	110	6.039367E-6	10.364991	17
ATAGGAC	325	0.0	10.230262	3
TCGAACT	130	3.8751932E-7	10.2247305	19
>>END_MODULE