FastQCFastQC Report
Thu 26 May 2016
SRR1513004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497446
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44450.1779818262336803No Hit
GTATCAACGCAGAGTACTTTTTTTT41810.16741102710529077No Hit
CTGTAGGACGTGGAATATGGCAAGA41660.16681041351845047No Hit
GTCCTAAAGTGTGTATTTCTCATTT40560.16240591388162146No Hit
CTTTAGGACGTGAAATATGGCGAGG39920.1598432959111028No Hit
TATCAACGCAGAGTACTTTTTTTTT30500.12212476265753094No Hit
GTCCTACAGTGTGCATTTCTCATTT29570.11840095841912097No Hit
GGTATCAACGCAGAGTACTTTTTTT25010.10014230537917536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8950.012.6517044
GTCCTAT2200.011.2388791
GGCGAGG11400.010.82216819
GACGTGG11250.010.645027
GTAGGAC26300.010.6286173
TGGCGAG24850.010.50182318
TGTAGGA26850.010.446312
AATGTCC7400.010.4020958
GGTATCA12550.010.2296881
AGGACGT25450.010.2070455
GTCCTAG4600.010.1300271
TCCAACG2250.010.12248518
GGACCTG18750.09.9447246
AGGACCT19150.09.937715
TTTAGAA8850.09.8839332
GGACGTG25200.09.8532146
GTCCTAC23850.09.8487451
CCACCTT8150.09.78269113
ACCTTTT8250.09.77916115
CTGTAGG27150.09.7374961
Produced by FastQC (version 0.11.5)