##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513004_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2497446
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.627149495925035	31.0	31.0	34.0	27.0	34.0
2	30.765001925967567	31.0	31.0	34.0	27.0	34.0
3	30.853668507747514	31.0	31.0	34.0	27.0	34.0
4	34.34904178108356	37.0	35.0	37.0	31.0	37.0
5	34.03657896907481	37.0	35.0	37.0	30.0	37.0
6	33.92587747642992	37.0	35.0	37.0	30.0	37.0
7	33.81285641411266	37.0	35.0	37.0	29.0	37.0
8	33.70324403410524	37.0	35.0	37.0	28.0	37.0
9	35.17795099473622	39.0	35.0	39.0	28.0	39.0
10	35.06400979240392	39.0	35.0	39.0	28.0	39.0
11	35.10548856712017	39.0	35.0	39.0	28.0	39.0
12	34.962813610384366	39.0	35.0	39.0	27.0	39.0
13	34.95444185780193	39.0	35.0	39.0	27.0	39.0
14	36.022865359251014	40.0	36.0	41.0	27.0	41.0
15	36.04796980595376	40.0	36.0	41.0	27.0	41.0
16	36.0285968145057	40.0	36.0	41.0	27.0	41.0
17	36.00149232455877	40.0	36.0	41.0	27.0	41.0
18	35.926194600403775	40.0	36.0	41.0	27.0	41.0
19	35.91238248995173	40.0	36.0	41.0	27.0	41.0
20	35.89046409812264	40.0	36.0	41.0	27.0	41.0
21	35.840006951101245	40.0	36.0	41.0	27.0	41.0
22	35.798809663952696	40.0	36.0	41.0	27.0	41.0
23	35.694484285145705	40.0	36.0	41.0	27.0	41.0
24	35.682528070677	40.0	36.0	41.0	27.0	41.0
25	35.59020134969885	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5089154428890517
1101	2	0.5868630442601237
1101	3	0.5565358437967305
1101	4	0.34131317115293314
1101	5	0.48334441133290085
1101	6	0.5774451356545356
1101	7	0.500879241186091
1101	8	0.512465199626412
1101	9	0.3343172431425785
1101	10	0.7414656883865476
1101	11	0.7897955439747548
1101	12	0.8370209194582614
1101	13	0.7990949494912343
1101	14	0.886419445342689
1101	15	0.7060065854380468
1101	16	0.8306350806591354
1101	17	0.895247870342395
1101	18	0.40350537026075983
1101	19	0.7297906801920178
1101	20	0.761152330992168
1101	21	0.8948816321588282
1101	22	0.8562880590061397
1101	23	0.7890044404103733
1101	24	0.9146845459934454
1101	25	0.876654331921209
1102	1	0.6248930894928861
1102	2	0.6207630187003836
1102	3	0.5200216835679505
1102	4	0.519868283167483
1102	5	0.6878110759476499
1102	6	0.562512494263018
1102	7	0.5972694299936023
1102	8	0.6585461958941536
1102	9	0.8777104522814554
1102	10	0.9269238032019658
1102	11	0.8307041056841271
1102	12	0.8618299239800606
1102	13	0.8841671256419943
1102	14	1.0660780421799743
1102	15	1.0371776898194653
1102	16	1.034499686309843
1102	17	0.9817829734980208
1102	18	0.9962707599664782
1102	19	1.1440330748316967
1102	20	1.1087495148710644
1102	21	0.9836265151929169
1102	22	1.07204075314975
1102	23	1.0112181278509098
1102	24	1.033361086837509
1102	25	1.0828365768691413
1103	1	0.5360238355821316
1103	2	0.4714246376170337
1103	3	0.5761317367130481
1103	4	0.5530501622240038
1103	5	0.5919313726717306
1103	6	0.6768776765471145
1103	7	0.5628502206698585
1103	8	0.6001702082021296
1103	9	0.766604204547555
1103	10	0.7760812231005048
1103	11	0.8548235531318866
1103	12	0.9011523974646707
1103	13	0.8509509488879914
1103	14	0.9490311065975803
1103	15	0.8121521298687213
1103	16	0.8612741021873944
1103	17	1.0166784308496446
1103	18	1.0791339970811578
1103	19	1.112422356964558
1103	20	1.1240929981452226
1103	21	0.9369519219472764
1103	22	1.1275494533323425
1103	23	1.177866393530671
1103	24	1.1556026536869837
1103	25	1.1558080313854973
1104	1	0.4805547680201414
1104	2	0.516331323402099
1104	3	0.5069707164819022
1104	4	0.4787300549390636
1104	5	0.5315394241633769
1104	6	0.513826008861578
1104	7	0.5353597345269634
1104	8	0.5424434199330577
1104	9	0.7104210776011755
1104	10	0.7570371857044051
1104	11	0.812052004125519
1104	12	0.7581843300979259
1104	13	0.8841030201723115
1104	14	0.9835626471543009
1104	15	0.9725930479359306
1104	16	0.8702880217044822
1104	17	0.8616603313166209
1104	18	0.9443835337907274
1104	19	0.8668371387299558
1104	20	1.081658154863817
1104	21	0.809469437366289
1104	22	0.9652909004628256
1104	23	0.8372235800534824
1104	24	0.9406470943618075
1104	25	0.9848316167893714
1105	1	0.41682949616415144
1105	2	0.534630789929512
1105	3	0.42917638501213773
1105	4	0.3503349268442051
1105	5	0.2927388927386687
1105	6	0.4325737049972318
1105	7	0.4946654266078099
1105	8	0.6249947193125465
1105	9	0.692946778487908
1105	10	0.585070735089019
1105	11	0.5751138910416174
1105	12	0.5241591974977311
1105	13	0.6426728835787472
1105	14	0.7757739217544497
1105	15	0.7021851615522792
1105	16	0.7657803831142402
1105	17	0.5883289690672271
1105	18	0.7618143154663031
1105	19	0.7445363717991142
1105	20	0.8855297989014161
1105	21	0.7276668939000501
1105	22	0.8333699639675629
1105	23	0.791982595842633
1105	24	0.7707422183609083
1105	25	0.8479481564875115
1106	1	0.3356681810397646
1106	2	0.3786803392733198
1106	3	0.32491113730348076
1106	4	0.3143231580186523
1106	5	0.3501056370521809
1106	6	0.3918264302492034
1106	7	0.4264521033228945
1106	8	0.5431538039932846
1106	9	0.34410881679368543
1106	10	0.48938717642759144
1106	11	0.2871316432416151
1106	12	0.509849911848697
1106	13	0.4010701613717558
1106	14	0.5955181058535928
1106	15	0.5482072832903526
1106	16	0.6324527591076645
1106	17	0.5572917366443448
1106	18	0.6069945018187255
1106	19	0.5656192027116944
1106	20	0.7530736084687248
1106	21	0.612304998708062
1106	22	0.6555562174312257
1106	23	0.7027842614435613
1106	24	0.5983624187202139
1106	25	0.6660320683659577
1107	1	0.4555799820255828
1107	2	0.44033217139551795
1107	3	0.4352658482972096
1107	4	0.4298279304395791
1107	5	0.4215255752768101
1107	6	0.4719436641848702
1107	7	0.42529959724235056
1107	8	0.39659733324705826
1107	9	0.3054075046887945
1107	10	0.5237904497264054
1107	11	0.5042469522925472
1107	12	0.4832081642324155
1107	13	0.5102189594379141
1107	14	0.6016300500866478
1107	15	0.5733002945163577
1107	16	0.5639818860456032
1107	17	0.48414307038552806
1107	18	0.6370046111939587
1107	19	0.7224974947868361
1107	20	0.7088128860649547
1107	21	0.6328145957754714
1107	22	0.7059538155530944
1107	23	0.7500952671087902
1107	24	0.8394511225814227
1107	25	0.6444400345039014
1108	1	0.4244358923811724
1108	2	0.3282418319072775
1108	3	0.355487153221862
1108	4	0.3809160617371816
1108	5	0.39367530388849303
1108	6	0.4082923461122121
1108	7	0.3587302598581843
1108	8	0.4187042763060802
1108	9	0.24793889728664453
1108	10	0.5200248996816867
1108	11	0.5741391108856533
1108	12	0.49145739726544235
1108	13	0.5055192416341683
1108	14	0.5602697322737811
1108	15	0.5707661305252358
1108	16	0.42631837880032464
1108	17	0.5710266212988486
1108	18	0.46625889817743627
1108	19	0.41681839436423473
1108	20	0.5465085196580048
1108	21	0.678637526055347
1108	22	0.6751081836456834
1108	23	0.6433701020646367
1108	24	0.6478162186927463
1108	25	0.6149601442094266
1109	1	-0.029711400216150707
1109	2	-0.03094329996743994
1109	3	-0.010833195019969821
1109	4	0.09824039523009986
1109	5	-0.03476518524797001
1109	6	-0.07603842021637774
1109	7	-0.11874124575508915
1109	8	0.1593822445572286
1109	9	0.12562491530112396
1109	10	0.060966014981310934
1109	11	0.12065089067912993
1109	12	0.08572624477648105
1109	13	0.09854958597635033
1109	14	0.26760506721558386
1109	15	0.29910751150826087
1109	16	0.0708620723069302
1109	17	0.06427965779386113
1109	18	0.03192448583551766
1109	19	0.3335669113879476
1109	20	0.39641083820543344
1109	21	0.3301898083011636
1109	22	0.36115574668207984
1109	23	0.24216862970934017
1109	24	-0.1934147388137646
1109	25	-0.00307994925532995
1110	1	0.15223285466202086
1110	2	0.1985087903166587
1110	3	0.14325037843064337
1110	4	0.18250077082407046
1110	5	0.1352609117088619
1110	6	0.1147988308199217
1110	7	0.09226269722337577
1110	8	0.277947922951725
1110	9	0.344526821074048
1110	10	0.3427289813143375
1110	11	0.23877412139392362
1110	12	0.3284867720234743
1110	13	0.0049938282167687476
1110	14	0.2895055322848421
1110	15	0.4077600744442691
1110	16	0.3385216239195046
1110	17	0.12521074413497502
1110	18	0.10861914512173598
1110	19	0.2185914918145997
1110	20	0.2888943459397382
1110	21	0.2315081153479781
1110	22	0.21054161514836522
1110	23	0.27525059816551334
1110	24	0.26754825784987446
1110	25	0.2705309091007493
1111	1	-0.35996871312356404
1111	2	-0.14624547067735705
1111	3	-0.24138158227713546
1111	4	-0.21181600643328125
1111	5	0.1316626486331387
1111	6	-0.07243282413714525
1111	7	0.12661086271908317
1111	8	0.21707019071128997
1111	9	-0.19300082659704287
1111	10	-0.08465522373379741
1111	11	0.05628729469602689
1111	12	0.02651321620770375
1111	13	0.11220173870336936
1111	14	0.2697455791232528
1111	15	0.35036676192800087
1111	16	0.06903408163483249
1111	17	0.18698295985186775
1111	18	0.08857051774729285
1111	19	0.17745744939981734
1111	20	0.10967644976487634
1111	21	0.09402193918351998
1111	22	-0.008301899033263282
1111	23	0.03218691094198789
1111	24	0.13181445613246012
1111	25	0.1729183668160772
1112	1	0.02676166194907026
1112	2	-0.18952221578650352
1112	3	-0.08522566326098868
1112	4	-0.24453400781645485
1112	5	-0.04552268863783837
1112	6	-1.824263197462983E-4
1112	7	-0.10540308272725696
1112	8	0.14386969322836762
1112	9	-0.1606000052813883
1112	10	0.1540696600115865
1112	11	-0.1415408075889104
1112	12	0.08007434821315229
1112	13	0.04332232854828533
1112	14	0.16602383378427277
1112	15	0.04985482125088936
1112	16	-0.09158645458282422
1112	17	-2.706837574351084E-4
1112	18	0.02431758877533241
1112	19	-0.036790090653987306
1112	20	0.24175647657457944
1112	21	0.3926416689623409
1112	22	0.19027508608245114
1112	23	0.3412517784564528
1112	24	0.22162775074416174
1112	25	0.2721335884210916
1113	1	-0.28454174841038693
1113	2	-0.6129249905310736
1113	3	-0.21381270229568017
1113	4	-0.3001219583903705
1113	5	-0.24136163457551163
1113	6	-0.3470258864049427
1113	7	-0.20060119054136294
1113	8	-0.008607112939358785
1113	9	-0.19023001989738475
1113	10	-0.560128253518819
1113	11	-0.22997274886675712
1113	12	-0.2884040733995903
1113	13	-0.10766656772974414
1113	14	-0.10198755520682568
1113	15	-0.06290340985236043
1113	16	-0.40169513032876125
1113	17	-0.29335478008741234
1113	18	-0.00959794330604069
1113	19	-0.24092290294235852
1113	20	-0.014663861079867502
1113	21	-0.1370153042997373
1113	22	-0.33953670498130606
1113	23	-0.07667683582636897
1113	24	-0.4214885080282542
1113	25	-0.384419546487095
1114	1	-0.4644687676080643
1114	2	-0.3478081578515706
1114	3	-0.3459812713603725
1114	4	-0.1339603269804428
1114	5	-0.2604988614188315
1114	6	-0.607999365878392
1114	7	-0.41972561772828954
1114	8	-0.7893323299561246
1114	9	-0.4557572850733962
1114	10	-0.6167129771690796
1114	11	-0.5313276714894215
1114	12	-0.5289540007287385
1114	13	-0.447240648452329
1114	14	-1.5620828523975092
1114	15	-0.8967018129881197
1114	16	-0.81562517311054
1114	17	-0.9085759547357171
1114	18	-1.0021284199774314
1114	19	-0.9273663329798225
1114	20	-0.2601913284261883
1114	21	-0.20845057489096774
1114	22	-0.33869401630636986
1114	23	-0.2378343505267253
1114	24	-0.9376286270449725
1114	25	-0.8775142590574916
1115	1	-1.5849631526980943
1115	2	-1.4485479956193181
1115	3	-1.0457723558958634
1115	4	-2.372956409720402
1115	5	-1.3771024717264524
1115	6	-1.5183455116470483
1115	7	-1.101139803441697
1115	8	-0.9948903208187758
1115	9	-1.511621201449671
1115	10	-1.4804121466452216
1115	11	-1.4037684985404582
1115	12	-1.8988578785928212
1115	13	-1.3733869468120545
1115	14	-2.1394261428060517
1115	15	-2.14074019158393
1115	16	-1.6891550077201103
1115	17	-1.667521420891184
1115	18	-1.5998319611121872
1115	19	-1.400781734664072
1115	20	-1.4443250940708978
1115	21	-1.5537974291230725
1115	22	-1.8025588880883134
1115	23	-2.23325399082092
1115	24	-1.6428334926156793
1115	25	-1.552194549797889
1116	1	-0.869306370002203
1116	2	-0.9113785941242973
1116	3	-0.678698753786346
1116	4	-0.8367327238558531
1116	5	-0.8234948662433439
1116	6	-1.3554120099266385
1116	7	-0.8847127083234554
1116	8	-1.0254572532213047
1116	9	-1.0778578379177546
1116	10	-1.2578400924690172
1116	11	-1.00025049486284
1116	12	-1.7755845039228006
1116	13	-1.4704989329902816
1116	14	-1.8486529362307706
1116	15	-1.7991015163458925
1116	16	-1.9431344680044447
1116	17	-1.881245844910893
1116	18	-2.7268209604076503
1116	19	-2.016590241134992
1116	20	-1.7321924541649167
1116	21	-1.7618227105721331
1116	22	-3.116400539918793
1116	23	-3.041190401311198
1116	24	-2.187252893379849
1116	25	-2.584060927633942
1201	1	0.7512357317233906
1201	2	0.6897189352665833
1201	3	0.6381960170589629
1201	4	0.6583571046847183
1201	5	0.7699670029220584
1201	6	0.7795497309636943
1201	7	0.8199949528559713
1201	8	0.8453919549161029
1201	9	1.0381021478653736
1201	10	0.9453044327251163
1201	11	1.0063555694719
1201	12	0.7937839250355623
1201	13	0.9047173996247437
1201	14	0.8911808399197909
1201	15	1.0057827963801742
1201	16	1.1293220695627681
1201	17	1.046692832406741
1201	18	1.1130197693518795
1201	19	0.9338439723595471
1201	20	1.0492810807660362
1201	21	0.9146735342753018
1201	22	1.03360150406197
1201	23	0.9917172815167703
1201	24	1.0147968458394416
1201	25	0.9430881619525806
1202	1	0.605091691286102
1202	2	0.6715718468400809
1202	3	0.6194711004592932
1202	4	0.5764528130230673
1202	5	0.6606250716004567
1202	6	0.8411102903630834
1202	7	0.6242385973395201
1202	8	0.627027239637755
1202	9	0.8336988110082331
1202	10	0.748434556227231
1202	11	0.851536655269264
1202	12	0.7495148885546428
1202	13	0.7387094689446059
1202	14	0.7384263607788384
1202	15	0.9521851615522792
1202	16	0.7822946107565159
1202	17	0.9066724650021882
1202	18	0.9866618764419073
1202	19	1.052463201067404
1202	20	1.0641375224786458
1202	21	1.0795469752008628
1202	22	1.0411951672195983
1202	23	1.0696756852735234
1202	24	0.8672869338080602
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2211	7	-0.14752587759186753
2211	8	-0.21125510257733993
2211	9	-0.47490741799284564
2211	10	-0.5314205705561932
2211	11	-0.3912710518908682
2211	12	-0.35302349030260416
2211	13	-0.33588291702878337
2211	14	-1.4685153905030361
2211	15	-0.915726959434565
2211	16	-0.4937491532212519
2211	17	-0.2972578056632358
2211	18	-0.5642341852079369
2211	19	-0.4552683911234183
2211	20	-0.6376467794639424
2211	21	-0.7329723200613074
2211	22	-0.4967281540538764
2211	23	-0.6320592491922739
2211	24	-0.6068106932489812
2211	25	-0.7670959399624735
2212	1	-0.14422506760912768
2212	2	-0.08392489332186415
2212	3	-0.12108253281200732
2212	4	1.4343446692066664E-4
2212	5	-0.07413654632269129
2212	6	-0.01118446000356954
2212	7	-0.0803112338144274
2212	8	-0.23956871504084631
2212	9	-0.26196059240237446
2212	10	-0.20567659870442867
2212	11	-0.10590314967451775
2212	12	-0.003708746195599133
2212	13	-0.14955352213654294
2212	14	-0.11302711206816696
2212	15	-0.08908326875455685
2212	16	0.051052929813941716
2212	17	-0.19318010281353537
2212	18	-0.033629049212422046
2212	19	-0.009351888881120374
2212	20	-0.22725851806083597
2212	21	-0.17450715310570786
2212	22	-0.054229428227721144
2212	23	-0.3848643889612191
2212	24	-0.051988424938457456
2212	25	-0.32548018356268926
2213	1	-0.25736912130403056
2213	2	-0.39080510469293017
2213	3	-0.32008114419554445
2213	4	-0.31483608701246624
2213	5	-0.2109035770086507
2213	6	-0.075790847639297
2213	7	-0.5438616754048553
2213	8	-0.18901164472281096
2213	9	-0.481158442320087
2213	10	-0.3113795387003506
2213	11	-0.2523293133390112
2213	12	-0.45282303231814325
2213	13	-0.2947531828412977
2213	14	-0.18944895010794482
2213	15	-0.26744955953785876
2213	16	-0.553932190296841
2213	17	-0.45394967431689537
2213	18	-0.6338063608561413
2213	19	-0.9189489303096892
2213	20	-0.3902222074939701
2213	21	-1.121257803573151
2213	22	-0.5768024345109168
2213	23	-0.6489361137943348
2213	24	-0.7332198900262483
2213	25	-0.6552096956244284
2214	1	-0.9721115891427274
2214	2	-0.8254629233464179
2214	3	-0.6437327730154969
2214	4	-1.1925257499490272
2214	5	-1.2354729753888734
2214	6	-2.5395027586139918
2214	7	-1.0497372516008596
2214	8	-1.6655674948130823
2214	9	-1.089916759779392
2214	10	-1.2631232870513642
2214	11	-1.0216863911739935
2214	12	-1.1163019093903372
2214	13	-1.4124383425738145
2214	14	-1.3861973881453764
2214	15	-1.7741489734306626
2214	16	-1.2585866522675602
2214	17	-1.3289740206271006
2214	18	-1.3081129572212546
2214	19	-3.1390989726480996
2214	20	-1.7327709933555724
2214	21	-2.1106379775957365
2214	22	-1.855344114703314
2214	23	-1.8082626499601773
2214	24	-1.0819917999787378
2214	25	-0.9815193199107881
2215	1	-1.1602333916984549
2215	2	-0.9815428620907625
2215	3	-1.0730534833059124
2215	4	-0.7996891813178024
2215	5	-1.571627685804735
2215	6	-1.3080729021479698
2215	7	-1.4558558508951762
2215	8	-2.3411726733323306
2215	9	-1.7991054547697658
2215	10	-1.1899354696193427
2215	11	-1.7948862620260755
2215	12	-2.5610810586501245
2215	13	-1.9258659103352755
2215	14	-1.8395539765823585
2215	15	-2.9944354864051377
2215	16	-2.49043881201208
2215	17	-2.1413593577716625
2215	18	-2.888443220931009
2215	19	-3.4510971261913284
2215	20	-2.8170874126862486
2215	21	-1.9977296607647972
2215	22	-2.9164978784776707
2215	23	-2.817028116709359
2215	24	-1.7943930060144666
2215	25	-1.9650059898976835
2216	1	-1.151315281865152
2216	2	-1.0204349912949482
2216	3	-1.4011350367333115
2216	4	-0.9479967663666997
2216	5	-0.8919665317563599
2216	6	-1.2720478717369872
2216	7	-2.44923839784423
2216	8	-1.763536710583132
2216	9	-1.8210159532283683
2216	10	-1.7002466845785662
2216	11	-2.115455181063311
2216	12	-1.5824743000717518
2216	13	-2.0515912220339416
2216	14	-2.144098890389394
2216	15	-2.214475207715971
2216	16	-1.96344729834599
2216	17	-2.098171913688091
2216	18	-1.9712760502418192
2216	19	-2.115402420287097
2216	20	-2.5618434722528107
2216	21	-2.395258323234252
2216	22	-2.6453222287137947
2216	23	-1.8031463244977957
2216	24	-2.080022593679942
2216	25	-1.8882883194995301
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54618.0
3	0.0
4	828.0
5	7490.0
6	8929.0
7	7018.0
8	5733.0
9	5274.0
10	4461.0
11	4183.0
12	3794.0
13	3673.0
14	3553.0
15	3621.0
16	3702.0
17	3936.0
18	4242.0
19	5011.0
20	6733.0
21	8819.0
22	11154.0
23	13764.0
24	16523.0
25	19176.0
26	22120.0
27	24673.0
28	28092.0
29	33822.0
30	43089.0
31	54641.0
32	70278.0
33	90509.0
34	118590.0
35	155289.0
36	213673.0
37	334953.0
38	1101482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14766456709195	19.74428414226669	13.602687477007933	27.505363813633423
2	13.516528485500787	20.893464763602495	40.36487675809607	25.225129992800642
3	16.891216066333367	25.006947097154452	30.373509577384254	27.728327259127926
4	11.519062325897343	17.591883146402235	37.946458789513166	32.94259573818725
5	12.632437295015459	38.21871562721012	36.15313259457088	12.995714483203539
6	31.48543235681409	37.80506375172212	18.0683176101259	12.641186281337886
7	27.41529503939039	31.73890508013465	22.8093751852558	18.036424695219157
8	24.60995706813205	37.541885115683655	20.666836283939315	17.18132153224498
9	26.06933748666032	14.97084499053003	20.302550322235362	38.65726720057429
10	14.489191388384501	29.148137904496206	35.0406249874738	21.322045719645498
11	33.80608797568942	22.29792471937242	25.271638044982904	18.624349259955256
12	23.07869429499899	24.809195917819792	32.06741964484151	20.044690142339704
13	28.728720952721503	22.09004661339155	25.957184232412665	23.224048201474282
14	20.557846234301667	22.293427471620568	27.794887237073034	29.353839057004734
15	22.5191243176751	31.529577022628843	23.252463417134365	22.698835242561696
16	21.84680445482105	28.154477047099203	28.019999439342907	21.97871905873684
17	21.39516590589501	28.88801577374623	27.948656023473177	21.76816229688558
18	21.319396339436942	27.641014963382354	29.984784358320354	21.054804338860347
19	23.29831024265236	27.067269960759187	27.583807159445822	22.050612637142628
20	23.446500747588857	27.45116647086005	28.091842512218896	21.010490269332198
21	22.621608332359003	26.807447934768508	27.323135173401287	23.247808559471203
22	23.05415823637075	28.21111689925808	27.22879139086769	21.50593347350348
23	21.954789012455127	27.958362262887764	27.872754806310123	22.214093918346983
24	22.92410843882448	27.033628368192293	28.378803136805814	21.663460056177414
25	22.496133976242476	27.487769270256486	27.95970045623485	22.056396297266197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	147.0
2	314.5
3	482.0
4	482.0
5	482.0
6	1181.0
7	1880.0
8	1880.0
9	1880.0
10	2922.5
11	3965.0
12	3965.0
13	3965.0
14	7356.0
15	10747.0
16	10747.0
17	10747.0
18	20098.0
19	29449.0
20	29449.0
21	29449.0
22	47463.0
23	65477.0
24	65477.0
25	65477.0
26	94229.5
27	122982.0
28	122982.0
29	122982.0
30	158247.0
31	193512.0
32	193512.0
33	193512.0
34	219998.5
35	246485.0
36	246485.0
37	246485.0
38	266530.5
39	286576.0
40	286576.0
41	286576.0
42	301604.5
43	316633.0
44	316633.0
45	316633.0
46	321752.0
47	326871.0
48	326871.0
49	326871.0
50	313223.5
51	299576.0
52	299576.0
53	299576.0
54	265503.0
55	231430.0
56	231430.0
57	231430.0
58	197671.0
59	163912.0
60	163912.0
61	163912.0
62	133267.0
63	102622.0
64	102622.0
65	102622.0
66	78884.5
67	55147.0
68	55147.0
69	55147.0
70	40173.0
71	25199.0
72	25199.0
73	25199.0
74	17397.5
75	9596.0
76	9596.0
77	9596.0
78	6506.0
79	3416.0
80	3416.0
81	3416.0
82	2216.0
83	1016.0
84	1016.0
85	1016.0
86	640.0
87	264.0
88	264.0
89	264.0
90	159.0
91	54.0
92	54.0
93	54.0
94	30.0
95	6.0
96	6.0
97	6.0
98	4.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0808826296944959
2	0.0
3	0.0
4	0.028629247639388396
5	0.07331489850030792
6	0.07944115708607913
7	0.03595673339884026
8	0.06530671734243704
9	0.04540638716512789
10	0.10714946389231239
11	0.0038839678615673772
12	0.010050267353127956
13	0.003803886049988668
14	0.005085195035248009
15	0.004084172390514149
16	0.015055380576797254
17	0.025586138799397466
18	6.406544926296705E-4
19	0.0018418816663103026
20	0.0033634360863057703
21	8.008181157870881E-5
22	2.00204528946772E-4
23	0.0
24	4.0040905789354407E-5
25	0.0019219634778890114
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2497446.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.69490891541236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48765260484933	58.31457087546001
2	12.003018877237555	16.97104652472568
3	2.9474344890429793	6.251058382111152
4	1.04690069788506	2.9604219792186375
5	0.4818006279262839	1.7030425753318559
6	0.26760608996052476	1.1351032892981365
7	0.1594392834680015	0.7890081935616287
8	0.10799474659384163	0.6107743019035738
9	0.07783974911228587	0.4952586576142439
>10	0.3823393302090834	4.673862102969214
>50	0.02031422620490169	1.00944923582957
>100	0.014542368444794108	2.113246727580862
>500	0.001813474365326839	0.9555560670254201
>1k	0.0013034347000575632	2.017601087370024
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4445	0.1779818262336803	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4181	0.16741102710529077	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4166	0.16681041351845047	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4056	0.16240591388162146	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3992	0.1598432959111028	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3050	0.12212476265753094	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2957	0.11840095841912097	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2501	0.10014230537917536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0040905789354407E-5
2	0.0	0.0	0.0	0.0	4.0040905789354407E-5
3	0.0	0.0	0.0	0.0	4.0040905789354407E-5
4	0.0	0.0	0.0	0.0	4.0040905789354407E-5
5	0.0	0.0	0.0	0.0	4.0040905789354407E-5
6	0.0	0.0	0.0	0.0	4.0040905789354407E-5
7	0.0	0.0	0.0	0.0	4.0040905789354407E-5
8	0.0	0.0	0.0	4.0040905789354407E-5	4.0040905789354407E-5
9	0.0	0.0	0.0	1.2012271736806321E-4	4.0040905789354407E-5
10	0.0	0.0	0.0	1.2012271736806321E-4	4.0040905789354407E-5
11	0.0	0.0	0.0	1.2012271736806321E-4	4.0040905789354407E-5
12	0.0	0.0	0.0	1.2012271736806321E-4	8.008181157870881E-5
13	0.0	4.0040905789354407E-5	0.0	1.2012271736806321E-4	8.008181157870881E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	895	0.0	12.651704	4
GTCCTAT	220	0.0	11.238879	1
GGCGAGG	1140	0.0	10.822168	19
GACGTGG	1125	0.0	10.64502	7
GTAGGAC	2630	0.0	10.628617	3
TGGCGAG	2485	0.0	10.501823	18
TGTAGGA	2685	0.0	10.44631	2
AATGTCC	740	0.0	10.402095	8
GGTATCA	1255	0.0	10.229688	1
AGGACGT	2545	0.0	10.207045	5
GTCCTAG	460	0.0	10.130027	1
TCCAACG	225	0.0	10.122485	18
GGACCTG	1875	0.0	9.944724	6
AGGACCT	1915	0.0	9.93771	5
TTTAGAA	885	0.0	9.883933	2
GGACGTG	2520	0.0	9.853214	6
GTCCTAC	2385	0.0	9.848745	1
CCACCTT	815	0.0	9.782691	13
ACCTTTT	825	0.0	9.779161	15
CTGTAGG	2715	0.0	9.737496	1
>>END_MODULE