FastQCFastQC Report
Thu 26 May 2016
SRR1513003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987317
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44870.45446396648695403No Hit
CCCATGTACTCTGCGTTGATACCAC38630.3912623807753741No Hit
GTATCAACGCAGAGTACTTTTTTTT23070.2336635548663702No Hit
GAGTACATGGGAAGCAGTGGTATCA19180.1942638483891192No Hit
CTGTAGGACGTGGAATATGGCAAGA17560.17785574440630517No Hit
GTCCTACAGTGGACATTTCTAAATT16970.1718799534496013No Hit
GGTATCAACGCAGAGTACTTTTTTT14520.14706522829040725No Hit
GTCCTAAAGTGTGTATTTCTCATTT14500.14686265910543422No Hit
TATCAACGCAGAGTACTTTTTTTTT14340.14524210562565013No Hit
CATGTACTCTGCGTTGATACCACTG13920.14098815274121687No Hit
CTTTAGGACGTGAAATATGGCGAGG13050.1321763931948908No Hit
GCGTTGATACCACTGCTTCCCATGT12490.12650445601564644No Hit
TATCAACGCAGAGTACATGGGAAGC11860.12012352668899653No Hit
GTATCAACGCAGAGTACATGGGAAG11700.11850297320921244No Hit
ACGCAGAGTACATGGGAAGCAGTGG11150.11293232062245459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC655.4598808E-513.1509353
GTAGAAC1207.421477E-1012.6638623
AAGGCGT701.0960004E-412.20725256
GACGTGG4900.012.0134877
TGTAGGA9650.012.0077042
GACTTAC802.7692853E-511.9140261
GTAGGAC9250.011.7055163
CGAAATC658.003085E-411.69505313
GGACGTG8800.011.6523766
TAGGACC2650.011.4691594
TAGACAG1251.8339051E-811.3974765
ATAGTAC1001.9321778E-611.3974763
AGGACGT9300.011.3362015
GTCCTAC8250.011.2064061
CGTGGAA5350.011.181049
TAGGACG9850.011.0889154
TAGTACC600.005882769411.0808794
GTATAAC1053.3279484E-610.8928251
TATAGGG700.001493322110.8547392
AGGCGTG700.00149774710.8508917
Produced by FastQC (version 0.11.5)