##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513003_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987317
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.081646522849297	31.0	30.0	34.0	23.0	34.0
2	29.229184750186615	31.0	30.0	34.0	22.0	34.0
3	29.38671672826458	31.0	30.0	34.0	23.0	34.0
4	32.80533101324094	35.0	33.0	37.0	26.0	37.0
5	32.92313107137829	35.0	35.0	37.0	26.0	37.0
6	32.95702494740797	37.0	35.0	37.0	26.0	37.0
7	32.79312621984631	37.0	35.0	37.0	25.0	37.0
8	32.76726927623043	37.0	35.0	37.0	25.0	37.0
9	34.06404224782922	39.0	35.0	39.0	25.0	39.0
10	33.95786358383376	39.0	34.0	39.0	23.0	39.0
11	34.008149358311464	39.0	35.0	39.0	25.0	39.0
12	33.904636504790254	39.0	35.0	39.0	23.0	39.0
13	33.88939216077511	39.0	35.0	39.0	23.0	39.0
14	34.903955872328744	39.0	35.0	41.0	22.0	41.0
15	34.84623479591661	39.0	35.0	41.0	22.0	41.0
16	34.72140254852292	39.0	34.0	41.0	19.0	41.0
17	34.683997135671724	39.0	34.0	41.0	19.0	41.0
18	34.38953243993571	39.0	34.0	41.0	17.0	41.0
19	34.62328613808939	39.0	34.0	41.0	18.0	41.0
20	34.71221502313846	39.0	34.0	41.0	18.0	41.0
21	34.64760760728317	39.0	34.0	41.0	18.0	41.0
22	34.57352299210892	39.0	34.0	41.0	18.0	41.0
23	34.309093229428846	39.0	34.0	41.0	16.0	41.0
24	34.32767490076642	39.0	34.0	41.0	15.0	41.0
25	34.24000397035603	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.39196078837812465
1101	2	0.5755210650948825
1101	3	0.5732728470406059
1101	4	0.5865327771175117
1101	5	0.4820207085781263
1101	6	0.6151256768991686
1101	7	0.5997274461271971
1101	8	0.3738117956440803
1101	9	0.31404749200616067
1101	10	0.39657353542779106
1101	11	0.6784558797328515
1101	12	0.6630487283160988
1101	13	0.47506467951151166
1101	14	0.33737451024630616
1101	15	0.2825034882869062
1101	16	0.5940487118526789
1101	17	0.7375598244410355
1101	18	0.8148108329046408
1101	19	0.33270740431377277
1101	20	0.4760964156963823
1101	21	0.5119609373583174
1101	22	0.48591742287082695
1101	23	0.7348083380805335
1101	24	0.4901532931693424
1101	25	0.7906909200935459
1102	1	0.48046288332693265
1102	2	0.41135079582679523
1102	3	0.4656211214555839
1102	4	0.7070037686822062
1102	5	0.3577390639711453
1102	6	0.42978387939534457
1102	7	0.5652446109409368
1102	8	0.4939150887215362
1102	9	0.5844031337132947
1102	10	0.6746517335275684
1102	11	0.7088793769096142
1102	12	0.7913985925385418
1102	13	0.6664677290109182
1102	14	0.6301642004780916
1102	15	0.8075596089346462
1102	16	0.7334335274150305
1102	17	0.5829528244032929
1102	18	0.9209100537657591
1102	19	0.9071274909538403
1102	20	0.9235161981414421
1102	21	0.6685334367976665
1102	22	0.9499382591184329
1102	23	1.0108446549673502
1102	24	0.8636625711169827
1102	25	0.8678014672226766
1103	1	0.33129408200291977
1103	2	0.4299270584370838
1103	3	0.45114129264983305
1103	4	0.5180765789454327
1103	5	0.17486059712948787
1103	6	0.4960225604406361
1103	7	0.2658170153557293
1103	8	0.22231710004312077
1103	9	0.5866505853013777
1103	10	0.5508120910958496
1103	11	0.6658973923634761
1103	12	0.78944630344796
1103	13	0.8629257790409639
1103	14	0.8719043942311941
1103	15	0.806537081711177
1103	16	0.8813373466069123
1103	17	0.699057101097047
1103	18	1.0586834743828604
1103	19	1.0719546627700396
1103	20	0.8669356556749435
1103	21	0.7500003944102289
1103	22	0.9451903595158484
1103	23	1.2027297945897573
1103	24	1.010448989425491
1103	25	1.015081228562714
1104	1	0.5137917545657587
1104	2	0.7044101140612504
1104	3	0.5619513674444789
1104	4	0.8876811996206726
1104	5	0.4122135885987177
1104	6	0.8086046540574472
1104	7	0.9212283859153914
1104	8	0.9150003975940919
1104	9	0.822697678696521
1104	10	1.0675196066595944
1104	11	1.0086063392219131
1104	12	0.990617938480483
1104	13	0.9391973197093648
1104	14	1.0490176121743104
1104	15	1.2011120948524905
1104	16	1.109100663664023
1104	17	1.1044947542804096
1104	18	1.3538050394339365
1104	19	1.3390051190743861
1104	20	1.2368126023307155
1104	21	1.3232149176181025
1104	22	1.1919911625608322
1104	23	1.4653179047107798
1104	24	1.4236384333105008
1104	25	0.8921266816688131
1105	1	0.5953717519058301
1105	2	0.7186001975956593
1105	3	0.746934172908734
1105	4	0.9031898475538469
1105	5	0.4933071990712392
1105	6	0.4863138225765624
1105	7	0.6768202516016828
1105	8	0.8436763233440914
1105	9	0.6953884493790454
1105	10	0.8569609584097648
1105	11	0.7222080719751247
1105	12	0.8082165299851809
1105	13	0.8357413130215505
1105	14	0.8570176628396027
1105	15	1.1288671787985507
1105	16	1.1247030423997941
1105	17	1.0388629263397675
1105	18	1.2036672931530816
1105	19	1.0777799054884838
1105	20	1.243634684801151
1105	21	1.1448224008827168
1105	22	0.7969702947909312
1105	23	0.9613058463696902
1105	24	0.9774392806876264
1105	25	0.9807770214429752
1106	1	1.0312224687731621
1106	2	1.092220487870751
1106	3	1.0113558383258443
1106	4	1.0230741743369833
1106	5	1.008921193262374
1106	6	0.8306944941448506
1106	7	0.6404104895214147
1106	8	0.9776455653569087
1106	9	1.1107388816835453
1106	10	1.3107326235118606
1106	11	1.1193632836751775
1106	12	1.0307173647728263
1106	13	1.0452585222476856
1106	14	1.3898612540855737
1106	15	1.392278698658103
1106	16	1.4656248579736797
1106	17	1.346371814990647
1106	18	1.6737432535788983
1106	19	1.293756166017836
1106	20	1.3155667089354424
1106	21	1.171531405458822
1106	22	1.4170366997857968
1106	23	1.5999929511581144
1106	24	1.4734425904368962
1106	25	1.3454966903000383
1107	1	0.3309698425412151
1107	2	0.4355948953991522
1107	3	0.5987218964894687
1107	4	0.6104065325640207
1107	5	0.44753778620700757
1107	6	0.44238081020340303
1107	7	0.6419331512903526
1107	8	0.4511518670395631
1107	9	0.5725463736726155
1107	10	0.39354857377404784
1107	11	0.42731412615304265
1107	12	0.5565210363398236
1107	13	0.5169565346500846
1107	14	0.6616713804233427
1107	15	0.7752916291593763
1107	16	0.5296654228339293
1107	17	0.6421528706123922
1107	18	1.1274861863528685
1107	19	0.9163074238268649
1107	20	0.781505730955061
1107	21	0.7712645184000522
1107	22	0.7564764342302936
1107	23	0.7425958767235841
1107	24	0.5281679996548121
1107	25	0.39256892611411587
1108	1	0.49777373356641874
1108	2	0.5830362616786786
1108	3	0.570636331297564
1108	4	0.678655687979969
1108	5	0.5798118682766997
1108	6	0.7985615630679064
1108	7	0.8082583339665064
1108	8	0.8569463719186459
1108	9	0.9227342133532304
1108	10	0.8163792982341107
1108	11	0.7342148942202584
1108	12	0.8857012694293189
1108	13	0.849687825467619
1108	14	1.100638884218995
1108	15	1.056762878274128
1108	16	0.990231570946861
1108	17	0.9230979028960178
1108	18	1.2718749313518956
1108	19	1.0925695289277115
1108	20	1.0345242306774622
1108	21	0.9088973962021996
1108	22	0.8791976878744592
1108	23	1.0461642066031587
1108	24	1.0687376263549027
1108	25	1.1859702395223337
1109	1	0.44862666851026134
1109	2	0.3121525998859269
1109	3	0.40412859660801104
1109	4	0.5478811594781661
1109	5	0.3716056805250574
1109	6	0.450839588073201
1109	7	0.5690657084102426
1109	8	0.46279506867643505
1109	9	0.7758651733023711
1109	10	0.8157859521862179
1109	11	0.3444448158138158
1109	12	0.7570776203511258
1109	13	0.8371851766013307
1109	14	0.5142805455879227
1109	15	0.8149637682932038
1109	16	0.8300864130744259
1109	17	0.5165763941017758
1109	18	-1.147583640379402
1109	19	0.3113870750179828
1109	20	0.5869505921947322
1109	21	0.5763322340915806
1109	22	0.7157467448780608
1109	23	0.3212012907311532
1109	24	0.7646000972173752
1109	25	0.749447671181585
1110	1	0.14192833332355548
1110	2	0.05605338797276005
1110	3	0.07914753325485435
1110	4	0.017878661452414235
1110	5	0.2048386899208552
1110	6	0.12337097455807822
1110	7	0.2841336385944544
1110	8	0.1522018278506323
1110	9	0.3734948691878799
1110	10	0.5643685240246015
1110	11	0.4219654043809129
1110	12	-0.15221624417529966
1110	13	0.19277511358662025
1110	14	0.3008971711580415
1110	15	0.44184816610783173
1110	16	0.5397590105377219
1110	17	0.36946080803270576
1110	18	0.7426672097551688
1110	19	0.3955528149976786
1110	20	0.523808559285257
1110	21	0.5581953583965671
1110	22	0.6112337793185816
1110	23	0.48613706729483397
1110	24	0.6647914782227105
1110	25	0.6948690317192288
1111	1	0.3270619719455645
1111	2	0.6945107822441088
1111	3	0.5937385362179519
1111	4	0.518267754864759
1111	5	0.5991549330743098
1111	6	0.7856678229014378
1111	7	0.7088290981736378
1111	8	0.5563501622315243
1111	9	0.9123293847665792
1111	10	0.8625337989340665
1111	11	0.6277509241199226
1111	12	0.8381611764346815
1111	13	0.8901502295663875
1111	14	0.8061798624872409
1111	15	1.0266705299999614
1111	16	0.9300047379927534
1111	17	0.9780777306031183
1111	18	1.15902709123133
1111	19	0.732642396273306
1111	20	0.6717837770279189
1111	21	0.9549209876803317
1111	22	0.7992706534019689
1111	23	0.9955800651099267
1111	24	1.1003366579539318
1111	25	0.628573500307418
1112	1	-0.06928951006581485
1112	2	0.32897220160400664
1112	3	0.3537413780735861
1112	4	0.5457986396291474
1112	5	0.5731936019099351
1112	6	0.46911695368559236
1112	7	0.10279276815253269
1112	8	0.3868481729325808
1112	9	0.6676750132086582
1112	10	0.49510718087292815
1112	11	0.5868175868667862
1112	12	0.5199729737287129
1112	13	0.3800232606830747
1112	14	0.5390495160421764
1112	15	0.553766970167473
1112	16	0.7223908514448567
1112	17	0.6161143350102805
1112	18	0.5478933868235103
1112	19	0.6597275670132987
1112	20	0.5045381231071389
1112	21	0.5061936163641292
1112	22	0.6067431004683286
1112	23	0.7649667469331547
1112	24	0.5019357733855401
1112	25	0.6736667262382028
1113	1	-0.4051645402809463
1113	2	-0.14750551977219217
1113	3	-0.29786163538222254
1113	4	-0.12256450596749602
1113	5	-0.1896824757630995
1113	6	-0.1890383118047012
1113	7	-0.25917604983151676
1113	8	0.004089329970696554
1113	9	-0.016302103864909157
1113	10	-0.2906365142362475
1113	11	-0.20644041007141567
1113	12	-0.03657927830577279
1113	13	-0.1709222715061145
1113	14	-0.1774838011764217
1113	15	-0.36026211693268095
1113	16	-0.3370137090256975
1113	17	-0.43639674387135585
1113	18	-0.33023931803508333
1113	19	-0.21755065391974426
1113	20	-0.3816696764255383
1113	21	-0.25942478666544133
1113	22	-0.17916778503646924
1113	23	-0.5949647654349022
1113	24	-0.09243897482169672
1113	25	-0.04445953272561809
1114	1	-1.2130634861188234
1114	2	-0.8891000914431011
1114	3	-0.7156600117302148
1114	4	-0.8006948828001654
1114	5	-0.928790678840766
1114	6	-1.372090535238602
1114	7	-1.3637489324196643
1114	8	-0.9308240348663368
1114	9	-0.8819788274805589
1114	10	-0.9114992308241554
1114	11	-1.6088092456463556
1114	12	-1.1246066622873556
1114	13	-1.2280917856221905
1114	14	-1.233952963905125
1114	15	-1.1996574883127877
1114	16	-2.1187342954791077
1114	17	-4.083516579082797
1114	18	-3.0979310664901867
1114	19	-1.8859803158608557
1114	20	-1.031154712170114
1114	21	-2.146093876693307
1114	22	-2.2044773694300233
1114	23	-1.8995170494528324
1114	24	-1.7089808383864664
1114	25	-1.4365084846218963
1115	1	-1.1817811644587053
1115	2	-0.7467065185237516
1115	3	-0.9359539359728686
1115	4	-0.9190613219223458
1115	5	-0.6326086499781027
1115	6	-0.7083161053191063
1115	7	-1.8220955097503122
1115	8	-1.0832418421985501
1115	9	-0.8165480149634305
1115	10	-0.7252617201238465
1115	11	-1.3755252724807931
1115	12	-0.948564506854126
1115	13	-0.8404462908516805
1115	14	-0.904452401350575
1115	15	-1.9733554141766732
1115	16	-0.7326108092890209
1115	17	-0.8818445559549914
1115	18	-0.8782253439061023
1115	19	-0.8332872856510889
1115	20	-0.7049588422791473
1115	21	-1.6190450092863244
1115	22	-1.4494417073394743
1115	23	-0.9040380876476846
1115	24	-0.7484504730539498
1115	25	-0.9628686641684538
1116	1	-1.2686485668029484
1116	2	-2.0958318207567217
1116	3	-1.74113268219757
1116	4	-1.4866774253968735
1116	5	-1.7501486200267102
1116	6	-1.7395044560683104
1116	7	-1.4659391601389267
1116	8	-1.9850627696877368
1116	9	-2.140517494662575
1116	10	-3.0136363133119985
1116	11	-1.6413309063543124
1116	12	-1.581116147905938
1116	13	-2.1516496172967514
1116	14	-2.11907713049127
1116	15	-1.7725727458255918
1116	16	-1.5806162808561126
1116	17	-1.8555187048920558
1116	18	-1.6918306381074117
1116	19	-2.3758086406587466
1116	20	-2.582734574958792
1116	21	-2.8302646500369484
1116	22	-2.069688178848004
1116	23	-2.0302430455233065
1116	24	-2.671126939459029
1116	25	-2.33000705131111
1201	1	-0.16859515298148509
1201	2	0.04930031486351538
1201	3	0.020985896486074296
1201	4	0.061021541088074116
1201	5	0.2095129228696777
1201	6	0.17283625628554233
1201	7	-0.015341010122391197
1201	8	-0.5482822665804079
1201	9	-0.13333545527200386
1201	10	0.08630306623983586
1201	11	-0.21603013566917895
1201	12	-0.8506185515279014
1201	13	-0.38913594590196965
1201	14	0.19221904490954955
1201	15	-0.15048782632784707
1201	16	0.35088303663394527
1201	17	0.4824835334014139
1201	18	0.6008754078284753
1201	19	0.26441917352898514
1201	20	0.21082439644361983
1201	21	0.15959901754255412
1201	22	0.39428462249497187
1201	23	0.5251496308482828
1201	24	0.3059284328692158
1201	25	0.1331073136155112
1202	1	0.5160612965884539
1202	2	0.5378190126810516
1202	3	0.34399912212209927
1202	4	0.7002612051750461
1202	5	0.6310687422158452
1202	6	0.5039241272963508
1202	7	0.4677074956658913
1202	8	0.5481974550689159
1202	9	0.8920304436755657
1202	10	1.0420870438338454
1202	11	0.7859845467099831
1202	12	0.7564365947100953
1202	13	0.7911836533720873
1202	14	1.0810043333513164
1202	15	0.6697111661099058
1202	16	0.7888640216045104
1202	17	0.9883903062622252
1202	18	1.2194447932331869
1202	19	0.9036186058633717
1202	20	0.9082250273305519
1202	21	0.8733302031192522
1202	22	1.0678729190339382
1202	23	1.2537983965955846
1202	24	1.2045404374052353
1202	25	1.0320955513542174
1203	1	0.093093605082462
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2211	15	-0.11759641961049994
2211	16	-0.03667322677681284
2211	17	0.0901475430546057
2211	18	-0.08011936165558353
2211	19	-0.06791415810004509
2211	20	-0.3027832108978501
2211	21	-0.13464349501391837
2211	22	-0.33933742032061076
2211	23	0.0839435103436017
2211	24	-0.06648719970961992
2211	25	0.0011505484569838131
2212	1	-0.5406716286124613
2212	2	-0.5723061983133277
2212	3	-0.5343623018065529
2212	4	-0.8503132034108525
2212	5	-0.7519458951257576
2212	6	-0.8689098736609964
2212	7	-0.7759626728776752
2212	8	-0.9804423554770239
2212	9	-0.7992816264627507
2212	10	-0.7588274750989683
2212	11	-0.9930331642214156
2212	12	-0.783639741167768
2212	13	-0.8756744828232144
2212	14	-0.7403142870603432
2212	15	-1.039988277116862
2212	16	-0.8464696644358511
2212	17	-0.8519644162329243
2212	18	-3.2302465881186855
2212	19	-1.680509577947376
2212	20	-1.1125796485568813
2212	21	-1.2745926044286762
2212	22	-0.9207623567073782
2212	23	-0.7977358789693767
2212	24	-1.4017289299895168
2212	25	-1.0281115380570114
2213	1	-0.11700750647505842
2213	2	-0.008692966761167042
2213	3	-0.37024016440197016
2213	4	-0.44954984463223013
2213	5	-0.3206481852020886
2213	6	-0.34855363956430097
2213	7	-0.08893977211432258
2213	8	-0.5459639839757529
2213	9	-0.3539890055976116
2213	10	-0.4865628440557117
2213	11	-0.5884530115496602
2213	12	-0.352342031244099
2213	13	-0.39984751081303216
2213	14	-0.4655051267549979
2213	15	-0.5838814068878548
2213	16	-1.0926528705427216
2213	17	-0.8411501668690562
2213	18	-0.5324094379914612
2213	19	-0.5736393489397358
2213	20	-1.2359893177680803
2213	21	-0.8191218665151894
2213	22	-0.672034621338419
2213	23	-1.1247078891474942
2213	24	-0.9271742226103825
2213	25	-1.1248036500162968
2214	1	-1.376732910748398
2214	2	-3.3142915981179044
2214	3	-1.6021808431876678
2214	4	-1.1826402304468928
2214	5	-1.2397101443228777
2214	6	-1.4029191913736518
2214	7	-1.374300558350516
2214	8	-0.9835704923680275
2214	9	-1.4065045212232903
2214	10	-1.299333179446677
2214	11	-1.0718110231484488
2214	12	-1.3925367560981172
2214	13	-1.3584830695256258
2214	14	-2.4196652486789674
2214	15	-2.6649686845750296
2214	16	-1.8615600902693217
2214	17	-2.4692378680886335
2214	18	-1.6965040624649603
2214	19	-4.343326101713544
2214	20	-2.4390164071667613
2214	21	-2.348622398431857
2214	22	-2.223192832364269
2214	23	-2.304893409675472
2214	24	-2.583917058409874
2214	25	-2.319415117405974
2215	1	-0.7415267572988995
2215	2	-2.421895319407895
2215	3	-1.1865943962461287
2215	4	-0.6661814718795469
2215	5	-0.5251807843761611
2215	6	-1.1369956368112035
2215	7	-1.6291057922202725
2215	8	-1.5325643260272237
2215	9	-1.3372180549447918
2215	10	-3.075950071353084
2215	11	-1.7597060879272561
2215	12	-2.0348202358909937
2215	13	-1.3712553446260713
2215	14	-2.73474635647176
2215	15	-2.58156838807502
2215	16	-2.4282132926842976
2215	17	-2.6048380657553025
2215	18	-1.5406374444987847
2215	19	-2.1536318404927357
2215	20	-2.3273579408023295
2215	21	-1.818592103865548
2215	22	-1.500989278661244
2215	23	-2.1888029127156727
2215	24	-1.8004587510381995
2215	25	-1.718786881151324
2216	1	-2.009586706808374
2216	2	-2.0457771247104652
2216	3	-2.54045329972827
2216	4	-3.294917610952183
2216	5	-2.3830139526033456
2216	6	-2.343747306513695
2216	7	-2.2583072110679367
2216	8	-2.754328937921258
2216	9	-3.093056953953088
2216	10	-2.7012630806522893
2216	11	-3.0571783660725274
2216	12	-3.6171826385582584
2216	13	-4.118580305479938
2216	14	-3.2809498737653016
2216	15	-3.1005832902363117
2216	16	-2.47760964330584
2216	17	-2.673738245774267
2216	18	-2.5691848299630493
2216	19	-3.039680051487739
2216	20	-2.7370210177417924
2216	21	-3.1919301240830578
2216	22	-3.970226914341211
2216	23	-4.1564165430270315
2216	24	-3.607348470783826
2216	25	-3.712823034071576
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46264.0
3	5.0
4	359.0
5	2347.0
6	3475.0
7	2959.0
8	2601.0
9	2438.0
10	2100.0
11	2077.0
12	1943.0
13	1855.0
14	1912.0
15	1789.0
16	1855.0
17	1997.0
18	2269.0
19	2668.0
20	3521.0
21	4402.0
22	5571.0
23	6825.0
24	7949.0
25	8627.0
26	9809.0
27	10912.0
28	12418.0
29	14727.0
30	18338.0
31	22812.0
32	28502.0
33	36761.0
34	47776.0
35	62728.0
36	86069.0
37	135256.0
38	383401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63161591387051	18.49005052748417	12.544761699536805	25.333571859108517
2	14.891838492324839	20.012259992907442	40.74512386645727	24.35077764831045
3	19.121944201277437	24.2514508964757	29.331817592322405	27.294787309924455
4	12.470731749326996	16.53074850733188	37.34147525154738	33.65704449179375
5	13.050033436683082	38.00808559790869	35.76306563722212	13.178815328186111
6	32.67246191235003	36.34107327193655	17.68296203847311	13.303502777240306
7	28.216157295926685	31.50021324380808	21.87901731580243	18.404612144462803
8	25.446066750956366	36.66409339946833	20.277649452116968	17.612190397458342
9	26.460332447239605	14.940371024734983	19.955344117742406	38.64395241028301
10	14.921016987957788	28.977186962763053	34.72334924846345	21.378446800815716
11	34.002753456256215	22.619673536124676	24.24084941795501	19.136723589664104
12	23.390300979627394	25.101761708421556	31.216885656107223	20.291051655843827
13	28.932511136032318	21.91759589347095	25.43543735203032	23.714455618466413
14	20.673364787552316	22.77975731553742	27.313399720174296	29.233478176735968
15	23.664046345142847	30.750160329635705	23.06558368599099	22.52020963923046
16	21.821709156666785	28.323109731079583	28.004700662043675	21.85048045020996
17	21.904100984722614	28.9602666450541	27.78741338088098	21.348218989342303
18	21.83357987490761	27.378179904715655	29.772556526288362	21.015683694088374
19	23.145182941346317	27.594839245642383	27.889551346669165	21.370426466342135
20	23.584488000478178	27.363914410266226	28.049369808556747	21.00222778069885
21	22.41866477822456	27.14686183270064	27.65615359599294	22.77831979308186
22	22.942138880868804	28.287770804524342	26.690169738781588	22.07992057582527
23	21.71093154814971	28.155848313422915	27.894372132981047	22.238848005446325
24	22.833370984914716	27.543431858910917	27.957869400002632	21.665327756171735
25	22.480006807917448	27.297520175547014	27.54187578387294	22.680597232662606
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	214.0
2	328.5
3	443.0
4	443.0
5	443.0
6	888.5
7	1334.0
8	1334.0
9	1334.0
10	1686.0
11	2038.0
12	2038.0
13	2038.0
14	3179.5
15	4321.0
16	4321.0
17	4321.0
18	7733.0
19	11145.0
20	11145.0
21	11145.0
22	17448.0
23	23751.0
24	23751.0
25	23751.0
26	34053.5
27	44356.0
28	44356.0
29	44356.0
30	56866.5
31	69377.0
32	69377.0
33	69377.0
34	79401.5
35	89426.0
36	89426.0
37	89426.0
38	97634.5
39	105843.0
40	105843.0
41	105843.0
42	112995.0
43	120147.0
44	120147.0
45	120147.0
46	130894.5
47	141642.0
48	141642.0
49	141642.0
50	134520.5
51	127399.0
52	127399.0
53	127399.0
54	111593.5
55	95788.0
56	95788.0
57	95788.0
58	81699.5
59	67611.0
60	67611.0
61	67611.0
62	55096.5
63	42582.0
64	42582.0
65	42582.0
66	32939.0
67	23296.0
68	23296.0
69	23296.0
70	16877.0
71	10458.0
72	10458.0
73	10458.0
74	7278.5
75	4099.0
76	4099.0
77	4099.0
78	2765.5
79	1432.0
80	1432.0
81	1432.0
82	925.0
83	418.0
84	418.0
85	418.0
86	284.5
87	151.0
88	151.0
89	151.0
90	91.0
91	31.0
92	31.0
93	31.0
94	19.5
95	8.0
96	8.0
97	8.0
98	7.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35409093533282626
2	0.037171445442547836
3	0.034538046037898666
4	0.03301877715060107
5	0.0381842913674129
6	0.018838934202490182
7	0.01863636501751717
8	0.024814725159194057
9	0.021573618199625853
10	0.02086462605222031
11	0.019649210942382235
12	0.020965910644706817
13	0.02086462605222031
14	0.027751978341302743
15	0.029169962636113832
16	0.022485179532004414
17	0.024004448419302007
18	0.10189230004142541
19	0.0255237173065996
20	0.0239031638268155
21	0.026333994046491654
22	0.02147233360713935
23	0.02319417167940996
24	0.019851780127355247
25	0.02339674086438297
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	987317.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.85344368485511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.41054363825381	62.34009330995782
2	11.117488945263403	16.4212968747202
3	2.6257506312574113	5.817621791281261
4	0.853930322785309	2.5226278001845985
5	0.3658055174562998	1.350799859153406
6	0.17405174787791017	0.7712592576091104
7	0.10100461579820003	0.5221677093333954
8	0.0675807354256434	0.39928560303510785
9	0.04598721976958673	0.30566830909286064
>10	0.20273033572602026	2.623283022969668
>50	0.01681030932244435	0.8589953654398146
>100	0.014735414517784772	2.4536722920336302
>500	0.001514855077222303	0.7877176461236939
>1k	0.002065711468939504	2.8255111590655058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4487	0.45446396648695403	No Hit
CCCATGTACTCTGCGTTGATACCAC	3863	0.3912623807753741	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2307	0.2336635548663702	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1918	0.1942638483891192	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1756	0.17785574440630517	No Hit
GTCCTACAGTGGACATTTCTAAATT	1697	0.1718799534496013	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1452	0.14706522829040725	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1450	0.14686265910543422	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1434	0.14524210562565013	No Hit
CATGTACTCTGCGTTGATACCACTG	1392	0.14098815274121687	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1305	0.1321763931948908	No Hit
GCGTTGATACCACTGCTTCCCATGT	1249	0.12650445601564644	No Hit
TATCAACGCAGAGTACATGGGAAGC	1186	0.12012352668899653	No Hit
GTATCAACGCAGAGTACATGGGAAG	1170	0.11850297320921244	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1115	0.11293232062245459	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.0128459248650636E-4	0.0
9	0.0	0.0	0.0	1.0128459248650636E-4	0.0
10	0.0	0.0	0.0	1.0128459248650636E-4	0.0
11	0.0	0.0	0.0	1.0128459248650636E-4	0.0
12	0.0	0.0	0.0	1.0128459248650636E-4	0.0
13	0.0	0.0	0.0	1.0128459248650636E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACAC	65	5.4598808E-5	13.150935	3
GTAGAAC	120	7.421477E-10	12.663862	3
AAGGCGT	70	1.0960004E-4	12.2072525	6
GACGTGG	490	0.0	12.013487	7
TGTAGGA	965	0.0	12.007704	2
GACTTAC	80	2.7692853E-5	11.914026	1
GTAGGAC	925	0.0	11.705516	3
CGAAATC	65	8.003085E-4	11.695053	13
GGACGTG	880	0.0	11.652376	6
TAGGACC	265	0.0	11.469159	4
TAGACAG	125	1.8339051E-8	11.397476	5
ATAGTAC	100	1.9321778E-6	11.397476	3
AGGACGT	930	0.0	11.336201	5
GTCCTAC	825	0.0	11.206406	1
CGTGGAA	535	0.0	11.18104	9
TAGGACG	985	0.0	11.088915	4
TAGTACC	60	0.0058827694	11.080879	4
GTATAAC	105	3.3279484E-6	10.892825	1
TATAGGG	70	0.0014933221	10.854739	2
AGGCGTG	70	0.001497747	10.850891	7
>>END_MODULE