FastQCFastQC Report
Thu 26 May 2016
SRR1513003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987317
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC44830.45405882811700804No Hit
GTACATGGGAAGCAGTGGTATCAAC44280.44848817553025017No Hit
GTATCAACGCAGAGTACTTTTTTTT30490.3088167224913579No Hit
TATCAACGCAGAGTACTTTTTTTTT22330.2261684950223687No Hit
GGTATCAACGCAGAGTACTTTTTTT18940.19183301816944306No Hit
CTGTAGGACGTGGAATATGGCAAGA17750.1797801516635488No Hit
GTCCTACAGTGGACATTTCTAAATT16820.1703606845623037No Hit
GAGTACATGGGAAGCAGTGGTATCA16480.16691700841776247No Hit
GTCCTAAAGTGTGTATTTCTCATTT14400.14584981318056914No Hit
CATGTACTCTGCGTTGATACCACTG14120.143013844590947No Hit
CTTTAGGACGTGAAATATGGCGAGG13370.135417500154459No Hit
TATCAACGCAGAGTACATGGGAAGC12420.1257954638682409No Hit
GCGTTGATACCACTGCTTCCCATGT12300.12458004875840283No Hit
ACGCAGAGTACATGGGAAGCAGTGG12200.12356720283353775No Hit
GTATCAACGCAGAGTACATGGGAAG11550.11698370432191485No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11290.11435030491726568No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9910.1003730311541278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGC250.00602599419.0024858
TAGGACC2400.013.4819764
ACTATCC907.4418695E-611.612638
GGCGAGG4050.011.48077419
TTAACCT1001.8867668E-611.4200254
GTAGGAC8400.011.2086943
GGACGTG8950.011.1619946
GACTGTT1303.2501703E-810.9635287
GTAATAC700.001477009510.8690373
GACGTGA5000.010.8319657
AGGACGT9250.010.8027275
TAGGACG9700.010.694014
ATAATAC1252.2022687E-710.6516563
TGTAGGA8950.010.6256142
TTAGGAC5650.010.6045263
ATTAGAC1002.3671513E-510.4614483
CAAATAC1002.3671513E-510.4614483
TAGTACA1201.4872221E-610.3097464
ACGTGAA5450.010.28574858
TTAGGGG750.002622174810.1444343
Produced by FastQC (version 0.11.5)