##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513003_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 987317 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.328764722981575 31.0 31.0 34.0 26.0 34.0 2 30.47729857786304 31.0 31.0 34.0 27.0 34.0 3 30.584672400049833 31.0 31.0 34.0 27.0 34.0 4 34.027205041541876 37.0 35.0 37.0 30.0 37.0 5 33.68034683895851 37.0 35.0 37.0 28.0 37.0 6 33.51380964776257 37.0 35.0 37.0 28.0 37.0 7 33.367331870108586 37.0 35.0 37.0 27.0 37.0 8 33.250016965169245 37.0 35.0 37.0 27.0 37.0 9 34.68823285732951 39.0 35.0 39.0 27.0 39.0 10 34.59029572062468 39.0 35.0 39.0 27.0 39.0 11 34.61264011457313 39.0 35.0 39.0 27.0 39.0 12 34.46087528119135 39.0 35.0 39.0 27.0 39.0 13 34.43904338728088 39.0 35.0 39.0 27.0 39.0 14 35.48200729856774 40.0 36.0 41.0 26.0 41.0 15 35.482794279851355 40.0 36.0 41.0 26.0 41.0 16 35.453221204537144 40.0 36.0 41.0 26.0 41.0 17 35.44367715738714 40.0 36.0 41.0 26.0 41.0 18 35.3760696919024 40.0 36.0 41.0 25.0 41.0 19 35.350055757168164 40.0 36.0 41.0 25.0 41.0 20 35.337655484510044 40.0 36.0 41.0 25.0 41.0 21 35.28251412666854 40.0 36.0 41.0 25.0 41.0 22 35.25406733602278 40.0 36.0 41.0 25.0 41.0 23 35.144452085804254 40.0 35.0 41.0 25.0 41.0 24 35.12515129386003 40.0 36.0 41.0 25.0 41.0 25 35.02905854958438 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31698171442281264 1101 2 0.3507031078739793 1101 3 0.372677502517476 1101 4 0.050427656627817896 1101 5 0.23283547724994236 1101 6 0.24426169923896168 1101 7 0.24288143814805352 1101 8 0.4300369054878317 1101 9 -0.11337857407524154 1101 10 0.45633689813904965 1101 11 0.3778923762989379 1101 12 0.5863789316924795 1101 13 0.5397505880916924 1101 14 0.5772418742217909 1101 15 0.5117902502533624 1101 16 0.6787566980528013 1101 17 0.48689716748237544 1101 18 -0.17603842157579663 1101 19 0.25854442483925766 1101 20 0.2858120358283145 1101 21 0.5635773528534784 1101 22 0.3836563170380032 1101 23 0.1999683620619237 1101 24 0.27130069388584843 1101 25 0.22484901577998784 1102 1 0.5625008331898691 1102 2 0.6142310149901427 1102 3 0.5422180348365302 1102 4 0.5000558503978212 1102 5 0.5073481546322682 1102 6 0.4327413681326888 1102 7 0.587889198451272 1102 8 0.6310018919766875 1102 9 0.7954401945344998 1102 10 0.7845162355268371 1102 11 0.5190792697130036 1102 12 0.6661557369344067 1102 13 0.7937791735405142 1102 14 0.9910394490992829 1102 15 0.8986099469909234 1102 16 0.7720766201611156 1102 17 0.7855487176556792 1102 18 0.9302117865868453 1102 19 0.8907882071599715 1102 20 0.8997890191464322 1102 21 0.8932314809129167 1102 22 0.810377960845635 1102 23 0.9129712552450684 1102 24 0.8617116679981862 1102 25 0.8659788448756842 1103 1 0.43049783939882147 1103 2 0.2680855209619146 1103 3 0.3252482995340422 1103 4 0.41189713730630473 1103 5 0.6374747582606588 1103 6 0.4977993991880041 1103 7 0.4508747671134472 1103 8 0.5606435857877585 1103 9 0.5374007060089383 1103 10 0.5503680428700548 1103 11 0.5731551475578982 1103 12 0.6270192980699392 1103 13 0.48976812602989384 1103 14 0.5322388629908943 1103 15 0.2158252996574035 1103 16 0.498604349623605 1103 17 0.7820004427750575 1103 18 0.8317603355469245 1103 19 0.7849155276115738 1103 20 0.7504747448772235 1103 21 0.4349031548005229 1103 22 0.6103589861136527 1103 23 0.7318152724066564 1103 24 0.6015301430622273 1103 25 0.6975738819291166 1104 1 0.5858465116513365 1104 2 0.6130300741256463 1104 3 0.5599894948551025 1104 4 0.4596390482747097 1104 5 0.5180401829941914 1104 6 0.5618327369545497 1104 7 0.5415339859812036 1104 8 0.4449227769162647 1104 9 0.5990502414714811 1104 10 0.7021625444185133 1104 11 0.7058310986405516 1104 12 0.5698042428764296 1104 13 0.6564924244738322 1104 14 0.9884102130311092 1104 15 0.9141223123047268 1104 16 0.8135005807586992 1104 17 0.7682600137666427 1104 18 0.8588624704559464 1104 19 0.8937331895347 1104 20 0.7233624769084628 1104 21 0.6984653052891261 1104 22 0.837909508916205 1104 23 0.6854286581785729 1104 24 0.8153947020524654 1104 25 1.0465640591943313 1105 1 0.8602713021819923 1105 2 0.8564350355250205 1105 3 0.8268664225113866 1105 4 0.8086608913516145 1105 5 0.7539796126295926 1105 6 0.6388997228125959 1105 7 0.8340462881490467 1105 8 0.9761775663702892 1105 9 1.0584363047144407 1105 10 0.9180055833231293 1105 11 0.9297894517650178 1105 12 0.9228121783288401 1105 13 1.0832956341205033 1105 14 1.1542453354828055 1105 15 1.2002913190748785 1105 16 1.1303195599795899 1105 17 0.7509324816100076 1105 18 1.174802406744334 1105 19 1.055465689423869 1105 20 1.1472384989225333 1105 21 1.1287542874866077 1105 22 1.232365458605564 1105 23 1.2567343662577883 1105 24 1.2293618582725827 1105 25 1.2600334288546833 1106 1 0.6180707389365914 1106 2 0.6476658800361079 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-2.0797459714432662 2216 15 -2.143989735953923 2216 16 -1.8984924917246389 2216 17 -2.2488845727122424 2216 18 -2.1524672236423115 2216 19 -2.278821484822423 2216 20 -2.670111059799872 2216 21 -2.664823688377183 2216 22 -2.8948928165321206 2216 23 -1.8780322518336448 2216 24 -2.1870161958162555 2216 25 -1.9993065788416544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27862.0 3 1.0 4 439.0 5 3911.0 6 5046.0 7 3823.0 8 3003.0 9 2487.0 10 2174.0 11 1957.0 12 1874.0 13 1660.0 14 1628.0 15 1631.0 16 1649.0 17 1762.0 18 1920.0 19 2380.0 20 3028.0 21 3843.0 22 4938.0 23 5882.0 24 6911.0 25 8023.0 26 9005.0 27 10072.0 28 11100.0 29 13235.0 30 16998.0 31 21453.0 32 27592.0 33 35430.0 34 46264.0 35 60687.0 36 83166.0 37 129454.0 38 425029.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.88821107306422 19.231993447555098 13.552126604913116 27.327668874467566 2 13.720848969173971 20.28643340777766 39.6043815803467 26.38833604270167 3 17.29211590603626 24.219475609150862 30.083043237379687 28.405365247433195 4 11.61102077412884 17.063355774052855 36.58191875750346 34.74370469431485 5 12.620656590791835 38.18378825458832 35.94859418537353 13.246960969246317 6 32.8506465333728 36.76573150111709 17.214432554952296 13.169189410557816 7 27.884926171463775 31.707210808258584 21.790285009661087 18.617578010616555 8 25.452182894234056 36.892752201240015 19.99193191182269 17.663132992703247 9 25.98503634508219 14.753073345825001 19.833379104592563 39.428511204500246 10 14.482120169576252 29.299661435598235 34.79395633644724 21.424262058378275 11 33.870539711608195 22.49915929615869 24.179452792959964 19.450848199273146 12 23.172846659724218 25.154179042185493 31.29024154041274 20.382732757677548 13 28.86869128869603 21.710293077915736 25.56850065738428 23.852514976003956 14 20.57137879269792 22.604044058807204 27.304892699341387 29.519684449153488 15 23.614712651129626 30.705143269261935 23.070868853521766 22.609275226086677 16 21.79068188955638 28.12260031810031 28.025144107546275 22.061573684797033 17 21.729029486531314 28.95795613545385 27.91265996820646 21.40035440980838 18 21.826341755907738 27.27176045546117 29.79346849561484 21.108429293016258 19 22.97042158509188 27.400600036058886 28.184880792971352 21.444097585877884 20 23.35257527705664 27.220682033885947 28.198243003566464 21.228499685490952 21 22.173875612139994 27.01711206656437 27.89768209740559 22.911330223890044 22 22.73564715546134 28.051489244039125 27.007747289866536 22.205116310633 23 21.469598923142215 27.898638431223205 28.17443637656396 22.457326269070624 24 22.804856803698108 27.49666773353212 28.172337934359415 21.52613752841036 25 22.320696944436285 27.054708841758323 27.715918770846393 22.908675442958994 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 133.0 1 133.0 2 255.5 3 378.0 4 378.0 5 378.0 6 890.5 7 1403.0 8 1403.0 9 1403.0 10 1769.5 11 2136.0 12 2136.0 13 2136.0 14 3160.0 15 4184.0 16 4184.0 17 4184.0 18 7631.5 19 11079.0 20 11079.0 21 11079.0 22 17679.5 23 24280.0 24 24280.0 25 24280.0 26 34721.0 27 45162.0 28 45162.0 29 45162.0 30 57439.0 31 69716.0 32 69716.0 33 69716.0 34 79556.5 35 89397.0 36 89397.0 37 89397.0 38 97666.0 39 105935.0 40 105935.0 41 105935.0 42 112611.0 43 119287.0 44 119287.0 45 119287.0 46 130504.0 47 141721.0 48 141721.0 49 141721.0 50 135159.0 51 128597.0 52 128597.0 53 128597.0 54 112293.5 55 95990.0 56 95990.0 57 95990.0 58 81584.5 59 67179.0 60 67179.0 61 67179.0 62 54519.5 63 41860.0 64 41860.0 65 41860.0 66 32350.0 67 22840.0 68 22840.0 69 22840.0 70 16529.0 71 10218.0 72 10218.0 73 10218.0 74 7072.0 75 3926.0 76 3926.0 77 3926.0 78 2633.5 79 1341.0 80 1341.0 81 1341.0 82 877.0 83 413.0 84 413.0 85 413.0 86 265.0 87 117.0 88 117.0 89 117.0 90 69.0 91 21.0 92 21.0 93 21.0 94 11.5 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08254694287650269 2 2.025691849730127E-4 3 0.0 4 0.02754940915632973 5 0.07454546007006868 6 0.08355978880136775 7 0.03727273003503434 8 0.07231719903536554 9 0.05327569564790235 10 0.11080534418023795 11 0.004962945031838812 12 0.015496542650435471 13 0.007191206066541952 14 0.009216897916272079 15 0.00860919036135304 16 0.020965910644706817 17 0.032816207965628064 18 0.0010128459248650635 19 0.004355237476919773 20 0.006482213919136406 21 2.025691849730127E-4 22 4.051383699460254E-4 23 0.0 24 1.0128459248650635E-4 25 0.003646245329514229 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 987317.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.75727993232985 #Duplication Level Percentage of deduplicated Percentage of total 1 83.68600055191017 60.887657685724385 2 11.628752829909843 16.92152849819247 3 2.7504579629427193 6.003475198557874 4 0.9044686909754556 2.632267269573167 5 0.36716800186300913 1.3357072546870583 6 0.1909214757313153 0.8334556352926096 7 0.11261554789137977 0.5735520659866071 8 0.06933808174639464 0.4035880178874564 9 0.046641180480661834 0.3054136882145249 >10 0.20778860958645481 2.6385030555052014 >50 0.01646322617973038 0.8607206472340737 >100 0.015618635218685756 2.622070544444764 >500 0.0015339726372342094 0.8005314256208481 >1k 0.0022312329268861226 3.181529013078952 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 4483 0.45405882811700804 No Hit GTACATGGGAAGCAGTGGTATCAAC 4428 0.44848817553025017 No Hit GTATCAACGCAGAGTACTTTTTTTT 3049 0.3088167224913579 No Hit TATCAACGCAGAGTACTTTTTTTTT 2233 0.2261684950223687 No Hit GGTATCAACGCAGAGTACTTTTTTT 1894 0.19183301816944306 No Hit CTGTAGGACGTGGAATATGGCAAGA 1775 0.1797801516635488 No Hit GTCCTACAGTGGACATTTCTAAATT 1682 0.1703606845623037 No Hit GAGTACATGGGAAGCAGTGGTATCA 1648 0.16691700841776247 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1440 0.14584981318056914 No Hit CATGTACTCTGCGTTGATACCACTG 1412 0.143013844590947 No Hit CTTTAGGACGTGAAATATGGCGAGG 1337 0.135417500154459 No Hit TATCAACGCAGAGTACATGGGAAGC 1242 0.1257954638682409 No Hit GCGTTGATACCACTGCTTCCCATGT 1230 0.12458004875840283 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1220 0.12356720283353775 No Hit GTATCAACGCAGAGTACATGGGAAG 1155 0.11698370432191485 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1129 0.11435030491726568 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 991 0.1003730311541278 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.0128459248650636E-4 0.0 0.0 0.0 0.0 9 1.0128459248650636E-4 0.0 0.0 0.0 0.0 10 1.0128459248650636E-4 0.0 0.0 0.0 0.0 11 1.0128459248650636E-4 0.0 0.0 0.0 0.0 12 1.0128459248650636E-4 0.0 0.0 0.0 0.0 13 1.0128459248650636E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGAGC 25 0.006025994 19.002485 8 TAGGACC 240 0.0 13.481976 4 ACTATCC 90 7.4418695E-6 11.61263 8 GGCGAGG 405 0.0 11.480774 19 TTAACCT 100 1.8867668E-6 11.420025 4 GTAGGAC 840 0.0 11.208694 3 GGACGTG 895 0.0 11.161994 6 GACTGTT 130 3.2501703E-8 10.963528 7 GTAATAC 70 0.0014770095 10.869037 3 GACGTGA 500 0.0 10.831965 7 AGGACGT 925 0.0 10.802727 5 TAGGACG 970 0.0 10.69401 4 ATAATAC 125 2.2022687E-7 10.651656 3 TGTAGGA 895 0.0 10.625614 2 TTAGGAC 565 0.0 10.604526 3 ATTAGAC 100 2.3671513E-5 10.461448 3 CAAATAC 100 2.3671513E-5 10.461448 3 TAGTACA 120 1.4872221E-6 10.309746 4 ACGTGAA 545 0.0 10.2857485 8 TTAGGGG 75 0.0026221748 10.144434 3 >>END_MODULE