FastQCFastQC Report
Thu 26 May 2016
SRR1513002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251934
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC34110.2724584522826283No Hit
CCCATGTACTCTGCGTTGATACCAC29110.23252024467743507No Hit
GTATCAACGCAGAGTACTTTTTTTT26060.2081579380382672No Hit
GTCCTACAGTGGACATTTCTAAATT19460.15543950399941212No Hit
GTCCTAAAGTGTGTATTTCTCATTT17470.1395440973725452No Hit
CTGTAGGACGTGGAATATGGCAAGA17400.1389849624660725No Hit
TATCAACGCAGAGTACTTTTTTTTT15730.12564560112593795No Hit
GGTATCAACGCAGAGTACTTTTTTT14680.11725857752884737No Hit
GAGTACATGGGAAGCAGTGGTATCA14550.11622018413111235No Hit
CTTTAGGACGTGAAATATGGCGAGG14220.11358426242916958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG456.774194E-414.7739855
GTAGAAC1550.012.8681793
AGGACGT9350.011.5799165
GTATAAT1655.456968E-1211.547271
GTAGGAC9900.011.4167183
GACGTGG5200.011.3203977
TAGGACC2700.011.2568214
GGACGTG9200.011.1457486
TAGGACG10150.011.1355184
TGCGCAT600.0058893111.07960310
GGTTTAC700.00145679710.8874251
TGTAGGA10450.010.8158382
GGCGAGG4000.010.68347619
ACGTGGA5600.010.6826238
CGTGGAA5650.010.5893559
CTGTAGG10850.010.5362181
GTCTTAG1451.3802492E-810.5119971
GGTTAAA1002.3247618E-510.4791461
ACTATAC1002.4030865E-510.4477363
ATTATAC1002.4030865E-510.4477363