##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513002_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1251934 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.902185738225818 31.0 30.0 34.0 23.0 34.0 2 29.107488893184463 31.0 30.0 34.0 22.0 34.0 3 29.268796917409386 31.0 30.0 34.0 23.0 34.0 4 32.703183234898965 35.0 33.0 37.0 25.0 37.0 5 32.79599803184513 35.0 35.0 37.0 26.0 37.0 6 32.85601637147006 37.0 35.0 37.0 26.0 37.0 7 32.67544614971716 37.0 33.0 37.0 25.0 37.0 8 32.683241289077536 37.0 35.0 37.0 25.0 37.0 9 33.974768637963344 39.0 35.0 39.0 23.0 39.0 10 33.87819405815323 39.0 34.0 39.0 23.0 39.0 11 33.90515154952258 39.0 34.0 39.0 23.0 39.0 12 33.81396543268255 39.0 34.0 39.0 22.0 39.0 13 33.79947105837848 39.0 34.0 39.0 22.0 39.0 14 34.799515789171 39.0 34.0 41.0 21.0 41.0 15 34.73213763664858 39.0 34.0 41.0 19.0 41.0 16 34.6396599181746 39.0 34.0 41.0 18.0 41.0 17 34.57804085518885 39.0 34.0 41.0 18.0 41.0 18 34.28975888505305 39.0 34.0 41.0 17.0 41.0 19 34.5163051726369 39.0 34.0 41.0 18.0 41.0 20 34.5920703487564 39.0 34.0 41.0 18.0 41.0 21 34.52697586294485 39.0 34.0 41.0 18.0 41.0 22 34.446407717978744 39.0 34.0 41.0 18.0 41.0 23 34.19894978489281 39.0 34.0 41.0 10.0 41.0 24 34.19722046050351 39.0 34.0 41.0 10.0 41.0 25 34.10885238359211 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3463255198430346 1101 2 0.473074958267091 1101 3 0.5645668844074407 1101 4 0.48346174272951714 1101 5 0.23055062616035826 1101 6 0.44717105186136763 1101 7 0.4224965159890317 1101 8 0.2479437915791607 1101 9 0.07770850442518196 1101 10 0.19757151952341445 1101 11 0.42099075950132914 1101 12 0.44062952398229527 1101 13 0.4201261549695232 1101 14 0.4178230699159968 1101 15 0.243200800863427 1101 16 0.629923873457436 1101 17 0.5982120214542945 1101 18 0.7480827746091592 1101 19 0.24622975497381105 1101 20 0.43371448991216965 1101 21 0.4199120096838769 1101 22 0.41837906396080626 1101 23 0.7038025122702578 1101 24 0.388251374697802 1101 25 0.48963817704297696 1102 1 0.8347348231802982 1102 2 0.9136055632671862 1102 3 0.8607095945394754 1102 4 1.0776893767479123 1102 5 0.8872789655522055 1102 6 0.9448376764621287 1102 7 1.002332992466492 1102 8 0.9556352350656709 1102 9 1.0321529215096845 1102 10 1.1738289054247701 1102 11 1.3045869534600456 1102 12 1.3271015896543545 1102 13 1.2066111482133266 1102 14 1.3143601735858894 1102 15 1.5316262123654738 1102 16 1.6300144177595115 1102 17 1.3222083647982927 1102 18 1.769351451615833 1102 19 1.547749206352293 1102 20 1.574184868843922 1102 21 1.383150766543956 1102 22 1.609127180485423 1102 23 1.8327063054865675 1102 24 1.687727551542885 1102 25 1.5595060593117864 1103 1 0.432696283626548 1103 2 0.7323043462286485 1103 3 0.7143820083325778 1103 4 0.9075828858756978 1103 5 0.5438935700146814 1103 6 0.7629543093221685 1103 7 0.6694901932778521 1103 8 0.5239384805017764 1103 9 0.8198304062967026 1103 10 0.8176585200292337 1103 11 0.8971518621821559 1103 12 1.1235468229200762 1103 13 1.052944819612918 1103 14 1.238089382510836 1103 15 1.2527185044547267 1103 16 1.321213200720969 1103 17 1.0460542065832783 1103 18 1.3632165631776942 1103 19 1.3664581313015844 1103 20 1.2126970392293188 1103 21 1.112546304069312 1103 22 1.1668219066517551 1103 23 1.6086464677583763 1103 24 1.2923472269992686 1103 25 1.2787454102245661 1104 1 0.2732518391821017 1104 2 0.3421253338829686 1104 3 0.2908202799375168 1104 4 0.6079841204436036 1104 5 0.24158492803937293 1104 6 0.32942961796414494 1104 7 0.4248667364877292 1104 8 0.4480187274153522 1104 9 0.5401390482720103 1104 10 0.47820172990577703 1104 11 0.500528405392032 1104 12 0.40715793403118994 1104 13 0.5311855603536628 1104 14 0.6788980244861449 1104 15 0.7213049242078142 1104 16 0.6095218426962745 1104 17 0.4570171695462406 1104 18 1.024901748362879 1104 19 0.8296741806843002 1104 20 0.7773822244145023 1104 21 0.7590401312298027 1104 22 0.7137416597381687 1104 23 0.9856279492398556 1104 24 0.8215138936659372 1104 25 0.5668318299776516 1105 1 0.3285873727354591 1105 2 0.4644380095949856 1105 3 0.45103052318406256 1105 4 0.6067165492420372 1105 5 0.2941212927869614 1105 6 0.10755331922315747 1105 7 0.4419208863471624 1105 8 0.6101810547592024 1105 9 0.23987991124720054 1105 10 0.5468070348807217 1105 11 0.4108498819841344 1105 12 0.3922844466824529 1105 13 0.3170784829792552 1105 14 0.4409161151841019 1105 15 0.665446227227001 1105 16 0.6551042898298789 1105 17 0.44959876103872176 1105 18 0.5418947809994776 1105 19 0.5992353590243553 1105 20 0.7039792174471344 1105 21 0.7452789773366391 1105 22 0.307123886849773 1105 23 0.5844434974613435 1105 24 0.6679165339299615 1105 25 0.763448380521595 1106 1 0.776017344009464 1106 2 0.8723306103425408 1106 3 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-1.8577939212321013 2215 3 -0.8914000707145249 2215 4 -0.5564736923592761 2215 5 -0.4181903498553794 2215 6 -0.9965955011813534 2215 7 -1.3098392598894435 2215 8 -1.2697845839270308 2215 9 -0.942175549306981 2215 10 -2.5281086700086632 2215 11 -1.4113080728476248 2215 12 -1.6702552886121396 2215 13 -1.067335364469379 2215 14 -2.398500492962903 2215 15 -2.1102655236990344 2215 16 -1.6306920510386433 2215 17 -2.059210547071295 2215 18 -0.7838676274016194 2215 19 -1.41186157633242 2215 20 -1.8433849856759394 2215 21 -1.5480481734618223 2215 22 -1.241589842130054 2215 23 -1.7644404299135275 2215 24 -1.5501663084636377 2215 25 -1.4766498252924904 2216 1 -0.8440365683590123 2216 2 -0.7675025129771278 2216 3 -1.3575836956385423 2216 4 -1.8106030347019164 2216 5 -0.9852724949672655 2216 6 -1.054206340497327 2216 7 -1.0404610702600507 2216 8 -1.507438775815917 2216 9 -1.5631118319704456 2216 10 -1.1834678337613767 2216 11 -1.5812614952185697 2216 12 -1.7890073287044714 2216 13 -1.8439234114340124 2216 14 -1.590851050702156 2216 15 -1.3457165135958178 2216 16 -0.6729348137194648 2216 17 -0.9567761183264665 2216 18 -1.034507263537101 2216 19 -1.5863992694204399 2216 20 -1.0761062098681577 2216 21 -1.4027533349198578 2216 22 -1.962858598763411 2216 23 -1.8679834961405462 2216 24 -1.715044469751632 2216 25 -1.8713087413999787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61470.0 3 1.0 4 525.0 5 3027.0 6 4099.0 7 3687.0 8 3187.0 9 3029.0 10 2707.0 11 2486.0 12 2435.0 13 2345.0 14 2308.0 15 2392.0 16 2437.0 17 2549.0 18 2956.0 19 3485.0 20 4505.0 21 5817.0 22 7228.0 23 8763.0 24 10049.0 25 11390.0 26 12475.0 27 14050.0 28 15898.0 29 18882.0 30 23236.0 31 29181.0 32 36858.0 33 46926.0 34 61012.0 35 80679.0 36 110712.0 37 173693.0 38 475455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00915704223207 18.988957354254556 12.917025428389556 25.084860175123826 2 14.627862747918366 20.161017084591904 41.26799955250835 23.943120614981382 3 18.737789369470008 24.39049726282111 29.831294532594043 27.04041883511484 4 12.21465784511139 16.746492153290504 38.269249208936614 32.76960079266149 5 12.867256029004107 38.06301536907598 36.158282384096644 12.911446217823269 6 32.09046264317539 37.06857639096939 17.94161375857934 12.899347207275872 7 27.723424857153812 31.800612928378776 22.295429561171403 18.18053265329601 8 24.862798362129233 37.074123639268954 20.6648157395386 17.39826225906322 9 26.224332128002608 15.199151208553108 20.341034560448428 38.23548210299585 10 14.515330090695846 29.326108771100046 35.16229698894904 20.99626414925507 11 33.80755083583931 22.81437049204427 24.678590945260595 18.699487726855832 12 22.81587449707111 25.222103624922905 31.89376096536781 20.068260912638173 13 28.49692251644201 22.169546015250003 25.921886884103824 23.41164458420417 14 20.417299537940718 22.77505630923056 27.937927612576512 28.86971654025221 15 23.098729969757382 31.13200084695317 23.48848822854951 22.280780954739942 16 21.668225938019564 28.689021637786194 28.052503369544112 21.590249054650133 17 21.451524245068054 29.106545353441415 28.016372172101157 21.425558229389374 18 21.419235076998348 27.811580890435543 29.82361890234884 20.945565130217265 19 22.799057206775327 27.812320230105463 28.19343240651965 21.19519015659955 20 23.099890142814342 27.87680015979227 28.32030360531309 20.703006092080294 21 22.392637791721032 27.416283884143333 27.839588488479773 22.351489835655862 22 22.600304074000395 28.520159882524855 27.372652035002865 21.50688400847189 23 21.581388808549466 28.32089892093056 28.08033338819495 22.017378882325026 24 22.472203647379988 27.710229265482827 28.40097372119202 21.416593365945165 25 22.132144272437905 27.686971889501066 28.07941950988789 22.101464328173137 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 181.0 2 316.5 3 452.0 4 452.0 5 452.0 6 946.5 7 1441.0 8 1441.0 9 1441.0 10 2028.5 11 2616.0 12 2616.0 13 2616.0 14 4450.0 15 6284.0 16 6284.0 17 6284.0 18 11067.0 19 15850.0 20 15850.0 21 15850.0 22 24408.5 23 32967.0 24 32967.0 25 32967.0 26 47101.5 27 61236.0 28 61236.0 29 61236.0 30 77977.5 31 94719.0 32 94719.0 33 94719.0 34 108514.0 35 122309.0 36 122309.0 37 122309.0 38 132127.0 39 141945.0 40 141945.0 41 141945.0 42 149157.0 43 156369.0 44 156369.0 45 156369.0 46 163920.5 47 171472.0 48 171472.0 49 171472.0 50 162201.5 51 152931.0 52 152931.0 53 152931.0 54 134169.0 55 115407.0 56 115407.0 57 115407.0 58 98713.0 59 82019.0 60 82019.0 61 82019.0 62 65696.0 63 49373.0 64 49373.0 65 49373.0 66 37572.0 67 25771.0 68 25771.0 69 25771.0 70 18794.5 71 11818.0 72 11818.0 73 11818.0 74 8159.5 75 4501.0 76 4501.0 77 4501.0 78 3018.5 79 1536.0 80 1536.0 81 1536.0 82 1013.5 83 491.0 84 491.0 85 491.0 86 329.0 87 167.0 88 167.0 89 167.0 90 109.0 91 51.0 92 51.0 93 51.0 94 30.0 95 9.0 96 9.0 97 9.0 98 14.0 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3664729929852532 2 0.04105647741813866 3 0.03698278024240895 4 0.03626389250551547 5 0.04193511798545291 6 0.018770957574440826 7 0.019010586820071985 8 0.024681812300009424 9 0.021726384937225125 10 0.022205643428487444 11 0.020608115124279715 12 0.021247126445962806 13 0.02092762078512126 14 0.028995138721370297 15 0.03107192551684034 16 0.022445272674118603 17 0.023803171732695173 18 0.08490862936864084 19 0.026678722680269086 20 0.024681812300009424 21 0.02819637456926643 22 0.02116725003075242 23 0.023962924563115946 24 0.01925021606570314 25 0.02412267739353672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1251934.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.19240757191419 #Duplication Level Percentage of deduplicated Percentage of total 1 85.19510497810279 64.0602505664552 2 10.66529983608319 16.03899144302873 3 2.374006312802137 5.355217507515466 4 0.760328346100029 2.2868367555373124 5 0.3268381950986317 1.228787538796256 6 0.1794834262745445 0.8097474564503496 7 0.09751938208021631 0.5132901986475794 8 0.07400539423429071 0.44517150126279803 9 0.052066412274272236 0.3523499003280954 >10 0.24305929608578386 3.218593801265653 >50 0.014940776175657745 0.8015759237708336 >100 0.014367710168205972 2.225093549223743 >500 0.001915672191561874 1.0412781014876433 >1k 0.0010642623286454857 1.6228157562302428 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3411 0.2724584522826283 No Hit CCCATGTACTCTGCGTTGATACCAC 2911 0.23252024467743507 No Hit GTATCAACGCAGAGTACTTTTTTTT 2606 0.2081579380382672 No Hit GTCCTACAGTGGACATTTCTAAATT 1946 0.15543950399941212 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1747 0.1395440973725452 No Hit CTGTAGGACGTGGAATATGGCAAGA 1740 0.1389849624660725 No Hit TATCAACGCAGAGTACTTTTTTTTT 1573 0.12564560112593795 No Hit GGTATCAACGCAGAGTACTTTTTTT 1468 0.11725857752884737 No Hit GAGTACATGGGAAGCAGTGGTATCA 1455 0.11622018413111235 No Hit CTTTAGGACGTGAAATATGGCGAGG 1422 0.11358426242916958 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.987641521038649E-5 0.0 4 0.0 0.0 0.0 7.987641521038649E-5 0.0 5 0.0 0.0 0.0 1.5975283042077298E-4 0.0 6 0.0 0.0 0.0 1.5975283042077298E-4 0.0 7 0.0 0.0 0.0 1.5975283042077298E-4 0.0 8 0.0 0.0 0.0 1.5975283042077298E-4 0.0 9 0.0 0.0 0.0 1.5975283042077298E-4 0.0 10 0.0 0.0 0.0 2.3962924563115947E-4 0.0 11 0.0 0.0 0.0 3.1950566084154597E-4 0.0 12 0.0 0.0 0.0 3.9938207605193243E-4 0.0 13 0.0 0.0 0.0 3.9938207605193243E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCCG 45 6.774194E-4 14.773985 5 GTAGAAC 155 0.0 12.868179 3 AGGACGT 935 0.0 11.579916 5 GTATAAT 165 5.456968E-12 11.54727 1 GTAGGAC 990 0.0 11.416718 3 GACGTGG 520 0.0 11.320397 7 TAGGACC 270 0.0 11.256821 4 GGACGTG 920 0.0 11.145748 6 TAGGACG 1015 0.0 11.135518 4 TGCGCAT 60 0.00588931 11.079603 10 GGTTTAC 70 0.001456797 10.887425 1 TGTAGGA 1045 0.0 10.815838 2 GGCGAGG 400 0.0 10.683476 19 ACGTGGA 560 0.0 10.682623 8 CGTGGAA 565 0.0 10.589355 9 CTGTAGG 1085 0.0 10.536218 1 GTCTTAG 145 1.3802492E-8 10.511997 1 GGTTAAA 100 2.3247618E-5 10.479146 1 ACTATAC 100 2.4030865E-5 10.447736 3 ATTATAC 100 2.4030865E-5 10.447736 3 >>END_MODULE