FastQCFastQC Report
Thu 26 May 2016
SRR1513002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251934
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC33820.2701420362415271No Hit
GTATCAACGCAGAGTACTTTTTTTT33410.26686710321790125No Hit
CCCATGTACTCTGCGTTGATACCAC32650.2607964956619119No Hit
TATCAACGCAGAGTACTTTTTTTTT23990.19162352008971717No Hit
GGTATCAACGCAGAGTACTTTTTTT20440.16326739269003No Hit
GTCCTACAGTGGACATTTCTAAATT18020.14393730020911646No Hit
CTGTAGGACGTGGAATATGGCAAGA17390.1389050860508621No Hit
GTCCTAAAGTGTGTATTTCTCATTT16200.12939979264082613No Hit
CTTTAGGACGTGAAATATGGCGAGG15800.12620473603241067No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12660.10112354165634929No Hit
GAGTACATGGGAAGCAGTGGTATCA12550.10024490108903504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA852.6778798E-713.4198441
GGCGTCA500.001509206113.28833712
TAGGACC3100.011.9712664
TTGCGAG658.0970203E-411.67925518
GGCGAGG5300.011.63793719
GTACTAG907.407365E-611.6181051
TGTCCAC3600.011.08468610
TCGCTGC600.005913057411.07361513
TGGCGAG10550.010.88357118
AGGACGT9450.010.8749965
CCTAGAC1053.4339919E-610.8649863
GTCTTAT1053.438936E-610.8636831
GACGTGG4500.010.7735827
GGACGTG9600.010.6029816
GTAGGAC9450.010.5631813
TGTAGGA9850.010.51944352
GGACCTG6950.010.4026556
AATGTCC2650.010.4020728
CCCCTAC1105.997743E-610.3698791
GTATAGG1105.997743E-610.3698791
Produced by FastQC (version 0.11.5)