##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513002_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1251934
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.28506854195189	31.0	31.0	34.0	26.0	34.0
2	30.44755713959362	31.0	31.0	34.0	27.0	34.0
3	30.53917698536824	31.0	31.0	34.0	27.0	34.0
4	33.99219527546979	37.0	35.0	37.0	30.0	37.0
5	33.64817314650772	37.0	35.0	37.0	28.0	37.0
6	33.514664511068474	37.0	35.0	37.0	28.0	37.0
7	33.36307904410297	37.0	35.0	37.0	27.0	37.0
8	33.23856768807301	37.0	35.0	37.0	27.0	37.0
9	34.67633277792599	39.0	35.0	39.0	27.0	39.0
10	34.5664212330682	39.0	35.0	39.0	27.0	39.0
11	34.592314770586945	39.0	35.0	39.0	27.0	39.0
12	34.44575752395893	39.0	35.0	39.0	27.0	39.0
13	34.427407515092646	39.0	35.0	39.0	27.0	39.0
14	35.46930029857804	40.0	36.0	41.0	26.0	41.0
15	35.480506160867904	40.0	36.0	41.0	26.0	41.0
16	35.44547556021324	40.0	36.0	41.0	26.0	41.0
17	35.42997793813412	40.0	36.0	41.0	26.0	41.0
18	35.36720306342028	40.0	36.0	41.0	25.0	41.0
19	35.34687052192847	40.0	36.0	41.0	25.0	41.0
20	35.33839004292559	40.0	36.0	41.0	25.0	41.0
21	35.276212643797514	40.0	36.0	41.0	25.0	41.0
22	35.24965693079667	40.0	36.0	41.0	25.0	41.0
23	35.13105243567153	40.0	36.0	41.0	25.0	41.0
24	35.10914553003593	40.0	36.0	41.0	24.0	41.0
25	35.0103248254301	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7037137342207984
1101	2	0.7594847386045878
1101	3	0.6935817306200072
1101	4	0.425369765111526
1101	5	0.757119039876855
1101	6	0.8483441084149774
1101	7	0.8633653882030146
1101	8	0.7760510168306425
1101	9	0.6650762190934998
1101	10	0.8559312241070245
1101	11	0.8509136280612779
1101	12	1.012592202249536
1101	13	0.9412310473313283
1101	14	1.1978243687830883
1101	15	1.0850129855886763
1101	16	1.2445336030171887
1101	17	1.1724214664819144
1101	18	0.8183232332027401
1101	19	1.042878927637382
1101	20	0.9880799640460864
1101	21	1.1083958808375343
1101	22	1.0472390710366568
1101	23	1.0929694126700724
1101	24	1.132006972117992
1101	25	1.1035472818633068
1102	1	0.9124527214173739
1102	2	0.9211245292600054
1102	3	0.9790153326621542
1102	4	0.9455475500028925
1102	5	1.1519753027210626
1102	6	1.1015862613276255
1102	7	1.216180366949473
1102	8	1.259120514840724
1102	9	1.54447839072035
1102	10	1.5408060164707251
1102	11	1.433948652546711
1102	12	1.461735032423313
1102	13	1.5783371796173284
1102	14	1.5978472998329636
1102	15	1.6496136518716256
1102	16	1.5898539246469667
1102	17	1.4794913092272353
1102	18	1.5462147770318282
1102	19	1.6632225650661852
1102	20	1.7046288461702304
1102	21	1.7768947229990673
1102	22	1.849333850657473
1102	23	1.9192395209436128
1102	24	1.7899957497545245
1102	25	1.8002620883447378
1103	1	0.9170571838920196
1103	2	0.8512513041078762
1103	3	0.8765690851976515
1103	4	0.9342514039582639
1103	5	1.0713089741206545
1103	6	1.1496389996643401
1103	7	1.0966377706005872
1103	8	1.1314410016561354
1103	9	1.2763597294627402
1103	10	1.4028131158622088
1103	11	1.342399971005129
1103	12	1.4271599817133733
1103	13	1.5960724737349707
1103	14	1.8057803627132856
1103	15	1.4935102035957613
1103	16	1.5251987522331731
1103	17	1.6030754877262225
1103	18	1.703398347011543
1103	19	1.7273483257152762
1103	20	1.8187008542838186
1103	21	1.6034525323296904
1103	22	1.7970579885885059
1103	23	1.9150387095845858
1103	24	1.9437330722697368
1103	25	1.9897276055861184
1104	1	0.6116250851265868
1104	2	0.6166525386757762
1104	3	0.5837542703828369
1104	4	0.6242884409953007
1104	5	0.7744509494293013
1104	6	0.6471698638618761
1104	7	0.8049896224524389
1104	8	0.7082558164709525
1104	9	0.8500942973639809
1104	10	0.945757560306653
1104	11	0.9409123166841411
1104	12	0.8489315866516449
1104	13	0.7500292638584298
1104	14	1.040138387404646
1104	15	0.9373744011266254
1104	16	1.1040259127270033
1104	17	0.9648162284669652
1104	18	1.0144415568880873
1104	19	1.0460149923819415
1104	20	1.0760156690986307
1104	21	0.9181315446753686
1104	22	0.9736505811810972
1104	23	0.8115448824240943
1104	24	1.0340725784425757
1104	25	1.1483634080552534
1105	1	0.5483443126048897
1105	2	0.5849889278702527
1105	3	0.5631235406431969
1105	4	0.5989345722750983
1105	5	0.553531746397887
1105	6	0.7473617719415699
1105	7	0.7626427210956379
1105	8	1.0856192194779197
1105	9	1.0798498284726463
1105	10	0.8744814326938908
1105	11	1.0088306640744378
1105	12	1.00828374408961
1105	13	0.9581566321508177
1105	14	1.12977046172319
1105	15	1.1025498075561586
1105	16	1.0328720195599033
1105	17	0.8680358837658275
1105	18	1.202749832160059
1105	19	1.2446948603687389
1105	20	1.176745408739265
1105	21	1.1940366907455342
1105	22	1.3129391767073173
1105	23	1.2867911848321114
1105	24	1.154401389101423
1105	25	1.3157622590514677
1106	1	0.7387655785901046
1106	2	0.7736997577161233
1106	3	0.7777737980106103
1106	4	0.80510118304516
1106	5	0.7787790281216402
1106	6	1.0198108209701857
1106	7	1.0695442507184083
1106	8	0.9452426707773967
1106	9	1.121054132997351
1106	10	1.2423440436972584
1106	11	0.9707266769452403
1106	12	1.2352157009082632
1106	13	1.0038331512018388
1106	14	1.2321186052267876
1106	15	1.3306142782153003
1106	16	1.4037579078541853
1106	17	1.426962085664364
1106	18	1.5400583862849828
1106	19	1.3772864700451706
1106	20	1.3973029652312903
1106	21	1.3762620561392183
1106	22	1.4194674142144237
1106	23	1.504429480325875
1106	24	1.6005163319653661
1106	25	1.544853526057402
1107	1	0.4706289576957907
1107	2	0.5357691871431598
1107	3	0.5151858082522658
1107	4	0.5708763435426079
1107	5	0.618655760007556
1107	6	0.5759222282772001
1107	7	0.7073378672656432
1107	8	0.6103283868663851
1107	9	0.32107819248835767
1107	10	0.6210617867176964
1107	11	0.583098617694354
1107	12	0.37389826107538227
1107	13	0.4558426525653232
1107	14	0.6635797827229553
1107	15	0.6002269749829026
1107	16	0.6371489697295516
1107	17	0.5993099004860127
1107	18	0.8070138617529636
1107	19	0.9083961749177902
1107	20	0.7995672148444726
1107	21	0.702621696176962
1107	22	0.838225702459944
1107	23	0.850124741484052
1107	24	0.9347178475234799
1107	25	0.9389472769249281
1108	1	0.21309234014201905
1108	2	0.23668099160787648
1108	3	0.08595189769765099
1108	4	0.19859906020826656
1108	5	0.1240511616206561
1108	6	0.15948274966434184
1108	7	0.11401667685058925
1108	8	0.2909410016561367
1108	9	0.019191760712743644
1108	10	0.15264124086220932
1108	11	0.016548408505130396
1108	12	0.1809138879633707
1108	13	0.11725606748497341
1108	14	0.18297958146328597
1108	15	0.1777992660957608
1108	16	0.08574562723317314
1108	17	0.12061845647622249
1108	18	0.18369912826154433
1108	19	0.07768816946527579
1108	20	0.11616179178381714
1108	21	0.32364393857969276
1108	22	0.28191736358850505
1108	23	0.12531995958458708
1108	24	0.25511979101973736
1108	25	0.17196198058611856
1109	1	0.3658102401267378
1109	2	0.41266328454797474
1109	3	0.32786003874288383
1109	4	0.40417071936168725
1109	5	0.38766716482970054
1109	6	0.1789299532095754
1109	7	0.38795439651745767
1109	8	0.6725119063016152
1109	9	0.6914514165043073
1109	10	0.5266431892118391
1109	11	0.6505552277288444
1109	12	0.7151587592194844
1109	13	0.8220736962288626
1109	14	0.8087314629577875
1109	15	0.9390285410040704
1109	16	0.7391963072454004
1109	17	0.8904813556968847
1109	18	0.8381428457890507
1109	19	1.0482969809719975
1109	20	1.047704521765482
1109	21	1.1209121900314045
1109	22	1.033576325996819
1109	23	1.009593721809523
1109	24	0.5024066664017681
1109	25	0.7412324955616683
1110	1	0.424896083695554
1110	2	0.4063937402571902
1110	3	0.36827438971631565
1110	4	0.4783516004219237
1110	5	0.40665376783381646
1110	6	0.471407172552361
1110	7	0.37964071755540374
1110	8	0.5906708641315817
1110	9	0.7412369396788563
1110	10	0.7790341374732108
1110	11	0.6796995780778232
1110	12	0.8278309050925472
1110	13	0.5531147134206336
1110	14	0.7363442134009119
1110	15	0.8427577478000856
1110	16	0.850100520406059
1110	17	0.6969831498087373
1110	18	0.4736547320803055
1110	19	0.7002717048901275
1110	20	0.6927922098830308
1110	21	0.5710183476538546
1110	22	0.5616670259165986
1110	23	0.7944552125315454
1110	24	0.6042389661793663
1110	25	0.7096008865291452
1111	1	-0.24999692432441378
1111	2	-0.07411442735504892
1111	3	-0.032533119211166195
1111	4	0.09881453021077391
1111	5	0.31308352321885025
1111	6	0.12224421008518505
1111	7	0.31784718943826107
1111	8	0.4586514224978231
1111	9	0.02906013026434806
1111	10	0.19962974912873932
1111	11	0.5024009730091379
1111	12	0.30893653481957983
1111	13	0.5162718725325703
1111	14	0.5721671362603828
1111	15	0.7172316464815296
1111	16	0.42474785042956853
1111	17	0.8915303975458642
1111	18	0.6792811125426041
1111	19	0.670630890845537
1111	20	0.557252958492235
1111	21	0.4947371014679689
1111	22	0.37700989239611715
1111	23	0.42736937090722904
1111	24	0.6374655371995956
1111	25	0.6457807117985439
1112	1	-0.13799497706177988
1112	2	-0.18707581958809882
1112	3	0.037694768257761524
1112	4	-0.16275356375162175
1112	5	-0.06400722061356845
1112	6	-0.13130486521394857
1112	7	-0.2486108632791897
1112	8	0.05214939708584154
1112	9	-0.2760583033241417
1112	10	-0.028979979021052316
1112	11	-0.17867628334161623
1112	12	0.14358596283607028
1112	13	-9.419293169088405E-5
1112	14	0.06593883265863809
1112	15	0.02528814696386661
1112	16	-0.3284400952581308
1112	17	-0.09399704083811145
1112	18	-0.26061134002273434
1112	19	-0.11185882778696055
1112	20	0.1811015000861005
1112	21	0.18131492676585736
1112	22	-0.13669660654313986
1112	23	0.07661893809924436
1112	24	0.1275420638246274
1112	25	0.11028572779178347
1113	1	-0.4317395805164743
1113	2	-0.8447370679851467
1113	3	-0.5077200957932497
1113	4	-0.5621722563046276
1113	5	-0.30877141010583387
1113	6	-0.46069467070977055
1113	7	-0.19131116772094714
1113	8	-0.10864191037622106
1113	9	-0.20814734839368043
1113	10	-0.46475361821261174
1113	11	-0.3206480573062933
1113	12	-0.4031519495303115
1113	13	-0.26821013496067536
1113	14	-0.28577979906629025
1113	15	-0.058302738770265705
1113	16	-0.5540479616192044
1113	17	-0.3167131877035061
1113	18	-0.26058496946974685
1113	19	-0.26918554688330687
1113	20	-0.04336941871921596
1113	21	-0.3325828569524134
1113	22	-0.47507952405052123
1113	23	-0.1815912196368501
1113	24	-0.5556122260113554
1113	25	-0.5294073792470471
1114	1	-0.7628919255227409
1114	2	-0.5133861004722746
1114	3	-0.4676904567870821
1114	4	-0.33427531359898666
1114	5	-0.5024353006885036
1114	6	-0.8685289392669588
1114	7	-0.7479309316894884
1114	8	-1.1292561993616772
1114	9	-0.7034557921657552
1114	10	-0.7340049502955495
1114	11	-0.7851229297582307
1114	12	-0.7888820030194523
1114	13	-0.6794835059087916
1114	14	-1.7666394846149558
1114	15	-0.999596666633245
1114	16	-1.004763079827896
1114	17	-0.906407972833577
1114	18	-1.0315673018434168
1114	19	-1.1463514198317952
1114	20	-0.45594927294264664
1114	21	-0.4513642615634339
1114	22	-0.6628096958898695
1114	23	-0.6154422064459482
1114	24	-1.3925073857455317
1114	25	-1.0950217582815682
1115	1	-1.8318537071970908
1115	2	-1.7119307640989447
1115	3	-1.3835211238232503
1115	4	-2.4376990910912397
1115	5	-1.6919027970564606
1115	6	-1.7697449387294988
1115	7	-1.5255861017866508
1115	8	-1.4776921116551947
1115	9	-2.096992855795783
1115	10	-1.79917093254263
1115	11	-1.629013120304002
1115	12	-2.3183389181766216
1115	13	-1.7025144349558943
1115	14	-2.5802405393769234
1115	15	-2.5084512925538576
1115	16	-2.0423204996920177
1115	17	-1.9195328731098584
1115	18	-1.6432897718003403
1115	19	-1.5270323123485312
1115	20	-1.6951467804796607
1115	21	-1.8142141343369715
1115	22	-2.14129624683504
1115	23	-2.442856779964366
1115	24	-1.8533543864761342
1115	25	-1.5573400511795583
1116	1	-2.1636396285616506
1116	2	-2.1592249746171035
1116	3	-2.1130098621707702
1116	4	-2.2277819540847297
1116	5	-2.397764042929012
1116	6	-2.8640155592682
1116	7	-2.2216287230984477
1116	8	-2.504601993154836
1116	9	-2.8261439769864793
1116	10	-2.833038255524002
1116	11	-2.9080333129088807
1116	12	-3.373817408946376
1116	13	-2.820454953989266
1116	14	-3.460915708450539
1116	15	-3.439449766752645
1116	16	-3.4231889423554094
1116	17	-3.3448199904057283
1116	18	-4.149425596650428
1116	19	-3.514943742483389
1116	20	-3.5501557061313065
1116	21	-3.4129292319401365
1116	22	-4.865435710447819
1116	23	-5.105826375663746
1116	24	-4.203377750561991
1116	25	-4.71101702006251
1201	1	0.8234147997860575
1201	2	0.9011608812856657
1201	3	0.8766944035878694
1201	4	0.8262936861793548
1201	5	0.97955324309671
1201	6	1.1019874459200736
1201	7	1.0916400630101677
1201	8	1.0567013174992326
1201	9	1.3269748593659543
1201	10	1.173508734812792
1201	11	1.2125469399302418
1201	12	1.138311535457639
1201	13	1.1693743076626362
1201	14	1.297145110548236
1201	15	1.2672335861734467
1201	16	1.3282425627273113
1201	17	1.149047469386943
1201	18	1.255473487103238
1201	19	1.0793992681503255
1201	20	1.327813182353097
1201	21	1.2920857467973477
1201	22	1.4456326192558535
1201	23	1.42976667698143
1201	24	1.542883607165308
1201	25	1.1110768159783788
1202	1	0.9080571838920193
1202	2	1.0097513041078763
1202	3	1.0205690851976499
1202	4	0.927251403958266
1202	5	0.9838089741206559
1202	6	0.9966389996643414
1202	7	1.0146377706005865
1202	8	0.8769410016561352
1202	9	1.1758597294627435
1202	10	1.165813115862207
1202	11	1.1468999710051335
1202	12	1.1556599817133701
1202	13	1.1250724737349742
1202	14	1.2292803627132827
1202	15	1.328510203595762
1202	16	1.321698752233175
1202	17	1.3930754877262217
1202	18	1.5033983470115473
1202	19	1.5763483257152728
1202	20	1.6157008542838156
1202	21	1.621452532329691
1202	22	1.4090579885885077
1202	23	1.5210387095845874
1202	24	1.3547330722697382
1202	25	1.1882276055861212
1203	1	0.7180001119317225
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2211	16	-0.17018719531754556
2211	17	0.05200225665250002
2211	18	-0.24428868910919022
2211	19	-0.13307126854498819
2211	20	-0.13418756728966486
2211	21	-0.35140694317748
2211	22	-0.13506274372223004
2211	23	-0.08395337354257748
2211	24	-0.14254723450908813
2211	25	-0.26622514057914515
2212	1	-0.13406471994479574
2212	2	-0.05224918156477898
2212	3	-0.04821090508889725
2212	4	0.21441021891698142
2212	5	0.061554481648585124
2212	6	-0.03113807658626655
2212	7	0.01959721693376082
2212	8	-0.13053180067509373
2212	9	-0.15977553037213
2212	10	-0.3469821731130196
2212	11	-0.05030983958253188
2212	12	-0.059791693857292216
2212	13	-0.12215992063122627
2212	14	-0.2449447465630641
2212	15	-0.12345579931827189
2212	16	0.02853775174749984
2212	17	-0.07657531363366132
2212	18	-0.017808306703848586
2212	19	-0.017154831156993566
2212	20	-0.17848831521593667
2212	21	0.06323446530686283
2212	22	-0.15673705755039435
2212	23	-0.495489216593171
2212	24	-0.12544007003235436
2212	25	-0.24551255954258266
2213	1	-0.15234860584896737
2213	2	-0.23126901077277395
2213	3	-0.13557007173480073
2213	4	-0.22985372554909844
2213	5	-0.16281469271530113
2213	6	0.1750224430366103
2213	7	-0.5941247992318139
2213	8	-0.19734619996905423
2213	9	-0.2938421496637176
2213	10	-0.2794080624008686
2213	11	-0.3604667125906005
2213	12	-0.46291035856088314
2213	13	-0.18208166542196125
2213	14	-0.1050947007707137
2213	15	-0.2863288009750846
2213	16	-0.5671704707226439
2213	17	-0.4801601648893197
2213	18	-0.5043566047406145
2213	19	-0.788452588454355
2213	20	-0.24419274652877476
2213	21	-1.0566383767612137
2213	22	-0.4610547589991043
2213	23	-0.528337115707437
2213	24	-0.7502245204169071
2213	25	-0.7116510130019975
2214	1	-1.0469681058866946
2214	2	-0.9823972733420696
2214	3	-0.7359093131163625
2214	4	-1.3147296287076955
2214	5	-1.2512194768804008
2214	6	-2.777568587269272
2214	7	-1.3279581198103685
2214	8	-1.9115748044555616
2214	9	-1.3614458553633924
2214	10	-1.6757574847700383
2214	11	-1.5502601975933956
2214	12	-1.5853990277702934
2214	13	-1.6959143545052768
2214	14	-1.9376053064121095
2214	15	-2.1247858975633562
2214	16	-1.501539920053446
2214	17	-1.7964092330324704
2214	18	-1.7331237815448333
2214	19	-3.509376647941206
2214	20	-2.206154256358964
2214	21	-2.5200448333183587
2214	22	-2.3081095561954754
2214	23	-2.3557168225545055
2214	24	-1.6252368961180395
2214	25	-1.2859357242347471
2215	1	-1.7995535361946082
2215	2	-1.5704582248580152
2215	3	-1.4630226852408974
2215	4	-1.2987475132047024
2215	5	-2.1372662830531404
2215	6	-2.0129273241039307
2215	7	-2.2224334259343337
2215	8	-3.0929585652090488
2215	9	-2.3783952786585374
2215	10	-1.8295585679493769
2215	11	-2.4185115070674215
2215	12	-3.0775804081079627
2215	13	-2.528879610726314
2215	14	-2.5493463292195813
2215	15	-3.577030673502236
2215	16	-3.003293397198334
2215	17	-2.733325703394513
2215	18	-3.403953304314932
2215	19	-4.191956492909522
2215	20	-3.6254044516176442
2215	21	-2.7106227248441
2215	22	-3.8563865160135506
2215	23	-3.649033299078109
2215	24	-2.7113061805727128
2215	25	-3.068103201127471
2216	1	-2.0366612276603284
2216	2	-1.8078551940870682
2216	3	-2.4330554635388104
2216	4	-1.9024236862944406
2216	5	-1.779245177503892
2216	6	-2.4635739967255503
2216	7	-3.6420968864391234
2216	8	-3.0490860741561363
2216	9	-3.11843268930982
2216	10	-3.099120097134179
2216	11	-3.513984505529166
2216	12	-3.1105674551097344
2216	13	-3.7543661544238702
2216	14	-3.7098477961314806
2216	15	-3.6324789660793293
2216	16	-3.42128139217116
2216	17	-3.8189570032485065
2216	18	-3.5530763822303335
2216	19	-3.557835789808191
2216	20	-3.8130229724309856
2216	21	-3.8565727384284294
2216	22	-4.215909520436767
2216	23	-3.3395858391518765
2216	24	-3.588153209318712
2216	25	-3.298100914269476
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36235.0
3	1.0
4	601.0
5	5030.0
6	6029.0
7	4661.0
8	3828.0
9	3102.0
10	2799.0
11	2567.0
12	2357.0
13	2103.0
14	1995.0
15	2099.0
16	2122.0
17	2296.0
18	2406.0
19	2994.0
20	3739.0
21	4862.0
22	6121.0
23	7435.0
24	8950.0
25	10042.0
26	11555.0
27	12573.0
28	13982.0
29	16998.0
30	21460.0
31	27713.0
32	34652.0
33	44869.0
34	58594.0
35	76944.0
36	105294.0
37	164139.0
38	538787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.17980512737705	19.85034025568938	14.007039331847022	26.962815285086545
2	13.552083058757935	20.689605593909576	39.93448531191366	25.82382603541883
3	17.06687413234244	24.474932384614526	30.4817186848508	27.976474798192235
4	11.442275654931553	17.4758677888015	37.33143807284288	33.75041848342406
5	12.441804321828496	38.370936694613384	36.28020899283106	12.907049990727062
6	32.14194355001167	37.60052376277831	17.44516958338796	12.812363103822062
7	27.43719034681157	31.97722550743168	22.238612753715838	18.346971392040913
8	24.838143140698556	37.298343420325814	20.38688965435097	17.476623784624657
9	25.732277599326725	14.981070157504922	20.234431133656596	39.052221109511756
10	14.159580065926578	29.684633605281803	35.05952104868363	21.09626528010799
11	33.77015001677504	22.74319812119566	24.559215886760498	18.9274359752688
12	22.50207915405393	25.343310077166375	31.86382479661839	20.290785972161306
13	28.36685671102633	22.18007002643366	25.933426158699426	23.51964710384059
14	20.221171812776458	22.612393322506144	27.968176694597403	29.198258170119995
15	22.884527664007273	31.200545778881267	23.50755598587302	22.407370571238445
16	21.680789493447786	28.625529106658636	28.02990960645171	21.663771793441875
17	21.29852031904472	29.076793507877834	28.17946025764571	21.445225915431735
18	21.40788300737754	27.765938072126705	29.834939947663884	20.99123897283187
19	22.61515910388983	27.614859548430132	28.466875157266617	21.303106190413427
20	22.93914741191169	27.76638984128607	28.451716391918275	20.84274635488396
21	22.13342599027102	27.3067184267491	28.005479539590873	22.554376043389006
22	22.43879450129001	28.346313292276726	27.53987842770762	21.675013778725646
23	21.3754878452059	28.144854281455732	28.18966495038876	22.28999292294961
24	22.343989379631836	27.63723966279373	28.545194874490186	21.473576083084254
25	22.089789971378977	27.530819721106788	28.144458281444585	22.234932026069654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	137.0
1	137.0
2	294.0
3	451.0
4	451.0
5	451.0
6	959.0
7	1467.0
8	1467.0
9	1467.0
10	2055.5
11	2644.0
12	2644.0
13	2644.0
14	4370.5
15	6097.0
16	6097.0
17	6097.0
18	10913.5
19	15730.0
20	15730.0
21	15730.0
22	24727.0
23	33724.0
24	33724.0
25	33724.0
26	47695.0
27	61666.0
28	61666.0
29	61666.0
30	79133.5
31	96601.0
32	96601.0
33	96601.0
34	109013.5
35	121426.0
36	121426.0
37	121426.0
38	131539.0
39	141652.0
40	141652.0
41	141652.0
42	149000.0
43	156348.0
44	156348.0
45	156348.0
46	164040.0
47	171732.0
48	171732.0
49	171732.0
50	162900.0
51	154068.0
52	154068.0
53	154068.0
54	135012.0
55	115956.0
56	115956.0
57	115956.0
58	98346.5
59	80737.0
60	80737.0
61	80737.0
62	64760.5
63	48784.0
64	48784.0
65	48784.0
66	37069.0
67	25354.0
68	25354.0
69	25354.0
70	18266.0
71	11178.0
72	11178.0
73	11178.0
74	7670.0
75	4162.0
76	4162.0
77	4162.0
78	2788.0
79	1414.0
80	1414.0
81	1414.0
82	927.0
83	440.0
84	440.0
85	440.0
86	290.0
87	140.0
88	140.0
89	140.0
90	81.0
91	22.0
92	22.0
93	22.0
94	13.0
95	4.0
96	4.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07692098784760219
2	7.987641521038649E-5
3	0.0
4	0.031231678347261115
5	0.07843863973659954
6	0.07875814539744108
7	0.042654005722346386
8	0.07851851615180992
9	0.05870916517963407
10	0.11598055488548119
11	0.0059108547255686
12	0.01781244059191619
13	0.009665046240456766
14	0.011741833035926814
15	0.01158208020550604
16	0.0236434189022744
17	0.03578463401425315
18	0.002076786795470049
19	0.006310236801620532
20	0.009744922655667152
21	3.1950566084154597E-4
22	3.1950566084154597E-4
23	0.0
24	0.0
25	0.00455295566699203
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1251934.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.1224702103618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.43438251723029	62.58485002863695
2	11.150298389221748	16.529753203834684
3	2.550064076725558	5.670511456848118
4	0.8259796997281577	2.4489462274985585
5	0.33114880863083435	1.227278385146791
6	0.18807073563276672	0.8364160499628348
7	0.11069328841593618	0.5743401981168051
8	0.06919768881066969	0.41032829019958
9	0.05184238951305487	0.34584173750838443
>10	0.2549525537259567	3.3025997608415008
>50	0.014817852986460076	0.7829536663681287
>100	0.015639794672315722	2.41860338671471
>500	0.0016178915034437392	0.8923804300240123
>1k	0.0012943132027549912	1.975197178298983
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3382	0.2701420362415271	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3341	0.26686710321790125	No Hit
CCCATGTACTCTGCGTTGATACCAC	3265	0.2607964956619119	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2399	0.19162352008971717	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2044	0.16326739269003	No Hit
GTCCTACAGTGGACATTTCTAAATT	1802	0.14393730020911646	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1739	0.1389050860508621	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1620	0.12939979264082613	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1580	0.12620473603241067	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1266	0.10112354165634929	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1255	0.10024490108903504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.987641521038649E-5	0.0
4	0.0	0.0	0.0	7.987641521038649E-5	0.0
5	0.0	0.0	0.0	1.5975283042077298E-4	0.0
6	0.0	0.0	0.0	1.5975283042077298E-4	0.0
7	0.0	0.0	0.0	1.5975283042077298E-4	0.0
8	0.0	0.0	0.0	1.5975283042077298E-4	0.0
9	0.0	0.0	0.0	2.3962924563115947E-4	0.0
10	0.0	0.0	0.0	3.9938207605193243E-4	0.0
11	0.0	0.0	0.0	5.591349064727055E-4	0.0
12	0.0	0.0	0.0	5.591349064727055E-4	0.0
13	0.0	0.0	0.0	5.591349064727055E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAA	85	2.6778798E-7	13.419844	1
GGCGTCA	50	0.0015092061	13.288337	12
TAGGACC	310	0.0	11.971266	4
TTGCGAG	65	8.0970203E-4	11.679255	18
GGCGAGG	530	0.0	11.637937	19
GTACTAG	90	7.407365E-6	11.618105	1
TGTCCAC	360	0.0	11.084686	10
TCGCTGC	60	0.0059130574	11.073615	13
TGGCGAG	1055	0.0	10.883571	18
AGGACGT	945	0.0	10.874996	5
CCTAGAC	105	3.4339919E-6	10.864986	3
GTCTTAT	105	3.438936E-6	10.863683	1
GACGTGG	450	0.0	10.773582	7
GGACGTG	960	0.0	10.602981	6
GTAGGAC	945	0.0	10.563181	3
TGTAGGA	985	0.0	10.5194435	2
GGACCTG	695	0.0	10.402655	6
AATGTCC	265	0.0	10.402072	8
CCCCTAC	110	5.997743E-6	10.369879	1
GTATAGG	110	5.997743E-6	10.369879	1
>>END_MODULE