FastQCFastQC Report
Thu 26 May 2016
SRR1513001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924847
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC52970.27519070346889907No Hit
CCCATGTACTCTGCGTTGATACCAC46780.24303230334670758No Hit
GTCCTACAGTGGACATTTCTAAATT34850.18105335125337235No Hit
CTGTAGGACGTGGAATATGGCAAGA34070.1770010811248894No Hit
GTCCTAAAGTGTGTATTTCTCATTT28490.1480117640518961No Hit
GTATCAACGCAGAGTACTTTTTTTT25350.13169877917569553No Hit
CTTTAGGACGTGAAATATGGCGAGG23580.1225032431149073No Hit
GAGTACATGGGAAGCAGTGGTATCA22570.11725607282033325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC250.0060508718.990058
TAGGACC4750.013.3957664
GACCGTC500.001506295813.2923437
GTGCGCC1352.7284841E-1112.66003211
CGTGCGC1001.4529905E-712.34353210
AGACCGT550.003082166712.0839486
GCGCCAC1157.046219E-811.56877813
GGACGTG17550.011.3609776
AGGACGT17900.011.3536585
GGCGAGG8000.011.15607419
GTCCTAA8500.011.0989961
TTAGGAC10150.011.0408453
GACGTGA9050.011.0157547
GTCCTAC16000.010.9588581
GTAGGAC17200.010.93259053
TAGGACG19150.010.8607984
GGACCGT700.001498864810.8508926
AGGACCG1158.857696E-710.7354575
ACGTGAA9850.010.6999768
GTACCGT803.7916747E-410.6813476
Produced by FastQC (version 0.11.5)