##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513001_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1924847
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.034530536712786	31.0	30.0	34.0	23.0	34.0
2	29.178212605988943	31.0	30.0	34.0	22.0	34.0
3	29.332912693840083	31.0	30.0	34.0	23.0	34.0
4	32.771810954325204	35.0	33.0	37.0	25.0	37.0
5	32.888924678169225	35.0	35.0	37.0	26.0	37.0
6	32.93503119988238	37.0	35.0	37.0	26.0	37.0
7	32.773227690304736	37.0	33.0	37.0	25.0	37.0
8	32.77139170022345	37.0	35.0	37.0	25.0	37.0
9	34.079335136766716	39.0	35.0	39.0	25.0	39.0
10	33.96300900798869	39.0	34.0	39.0	24.0	39.0
11	34.01599503752766	39.0	35.0	39.0	25.0	39.0
12	33.907053391776074	38.0	34.0	39.0	23.0	39.0
13	33.901641533067306	39.0	34.0	39.0	23.0	39.0
14	34.898998725613	39.0	34.0	41.0	23.0	41.0
15	34.83976336820537	39.0	34.0	41.0	22.0	41.0
16	34.72095392516912	39.0	34.0	41.0	19.0	41.0
17	34.67272723494387	39.0	34.0	41.0	19.0	41.0
18	34.36208955828697	39.0	34.0	41.0	17.0	41.0
19	34.5983540509973	39.0	34.0	41.0	18.0	41.0
20	34.67855055492722	39.0	34.0	41.0	19.0	41.0
21	34.613720986655046	39.0	34.0	41.0	18.0	41.0
22	34.5365231626202	39.0	34.0	41.0	18.0	41.0
23	34.26907593174938	39.0	34.0	41.0	16.0	41.0
24	34.270871918651196	39.0	34.0	41.0	15.0	41.0
25	34.1893438803188	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5671320890222127
1101	2	0.8868574869360799
1101	3	0.9219548492440666
1101	4	0.7760429620096687
1101	5	0.5810149224936509
1101	6	0.8390951793293837
1101	7	0.9684449251978648
1101	8	0.7328738153833783
1101	9	0.6727008641892311
1101	10	0.7382308670537086
1101	11	0.9375181940507531
1101	12	1.098995092299056
1101	13	0.9303879010939227
1101	14	0.9586596699830281
1101	15	0.5687286422002558
1101	16	1.1466656105994346
1101	17	1.1919696245133622
1101	18	1.4072331062340524
1101	19	0.8416190451651246
1101	20	0.9827493481694702
1101	21	1.0874253466369979
1101	22	0.9854980708070471
1101	23	1.1952501824019208
1101	24	0.7783032047865532
1101	25	1.1467293915965655
1102	1	0.664865035143972
1102	2	0.7846072516486053
1102	3	0.7996384224343096
1102	4	1.0097218444258331
1102	5	0.9400974798870934
1102	6	0.8183209942534617
1102	7	0.9956493413450929
1102	8	1.0561264386803515
1102	9	1.0303375987561552
1102	10	1.1815094785292857
1102	11	1.106236191210023
1102	12	1.2235971195152189
1102	13	1.0654380746364396
1102	14	1.0498115936957433
1102	15	1.270189786071775
1102	16	1.3707999427573867
1102	17	1.0210101469494788
1102	18	1.2714914121411809
1102	19	1.3534339899805587
1102	20	1.332351519380424
1102	21	1.1563661325259673
1102	22	1.360180216720991
1102	23	1.5948677322557927
1102	24	1.3626901383542673
1102	25	1.2562801624294408
1103	1	0.35855829144890095
1103	2	0.5005213834378246
1103	3	0.6395043609312907
1103	4	0.6043796604709968
1103	5	0.20695784401549133
1103	6	0.6125965369844693
1103	7	0.41687745068949766
1103	8	0.4202624971604223
1103	9	0.6759744269420125
1103	10	0.5708348431792345
1103	11	0.6367998077931318
1103	12	0.7380651776825857
1103	13	0.677339981249375
1103	14	0.9696804114082838
1103	15	0.9923309536950669
1103	16	1.0231753293844434
1103	17	0.8807672294705498
1103	18	1.2463690173187203
1103	19	1.1109449013739408
1103	20	0.9324711712882205
1103	21	0.7251903109075712
1103	22	0.8691688663486232
1103	23	1.1142984946689154
1103	24	0.8172593610719545
1103	25	0.7841525685727575
1104	1	0.5799373283797671
1104	2	0.6038448563419756
1104	3	0.6103378213320099
1104	4	0.8152009224026244
1104	5	0.5519988613678137
1104	6	0.5553527810758396
1104	7	0.7290936075618148
1104	8	0.7204802398337122
1104	9	0.6942757901209333
1104	10	0.9118906206791593
1104	11	0.8678534505609647
1104	12	0.7344044171080384
1104	13	0.883040325856129
1104	14	0.9589091631999338
1104	15	1.110634146644351
1104	16	0.9095722065763567
1104	17	0.888997536567004
1104	18	1.0689764400871624
1104	19	1.0675476277317841
1104	20	1.1390693232201485
1104	21	1.137991384372846
1104	22	1.0486510413356598
1104	23	1.4572242974806642
1104	24	1.2667485501787255
1104	25	1.0499572100902483
1105	1	0.39713628340991747
1105	2	0.5068958616051624
1105	3	0.5349614295449925
1105	4	0.6369582024140144
1105	5	0.3280398057839591
1105	6	0.23011862129622074
1105	7	0.45989053399220836
1105	8	0.6106419691821401
1105	9	0.39622399072447223
1105	10	0.549430450570334
1105	11	0.4540830700889771
1105	12	0.47775785022832196
1105	13	0.42828138638003566
1105	14	0.5881546270559141
1105	15	0.7645693782300427
1105	16	0.6360132298581505
1105	17	0.6020168312671217
1105	18	0.6571116508610899
1105	19	0.5790360525547484
1105	20	0.640973928645451
1105	21	0.6291510927890158
1105	22	0.32934374462008975
1105	23	0.6086654029141769
1105	24	0.5764999590806852
1105	25	0.6234665671709081
1106	1	0.3586502983721225
1106	2	0.39682716839231347
1106	3	0.4633487172386843
1106	4	0.4694222824862635
1106	5	0.3705617255890914
1106	6	0.2824777800627061
1106	7	0.21087877475575567
1106	8	0.3190290012449708
1106	9	0.4233978766884121
1106	10	0.5148310295525818
1106	11	0.3483002016463672
1106	12	0.4354279785268531
1106	13	0.378007135622191
1106	14	0.5504593646107523
1106	15	0.5719145845339
1106	16	0.595297407109129
1106	17	0.549701974629464
1106	18	0.7876646085860273
1106	19	0.3533623081626516
1106	20	0.41957950993379
1106	21	0.41166251873048054
1106	22	0.43208915512534674
1106	23	0.8300503979456266
1106	24	0.6905466368434645
1106	25	0.7282861621866203
1107	1	0.46134252707089374
1107	2	0.5002083326966513
1107	3	0.7462323647597771
1107	4	0.7409974070057572
1107	5	0.6950507122714669
1107	6	0.6391215254588545
1107	7	0.8015437447694964
1107	8	0.6601105657986324
1107	9	0.8662900095838921
1107	10	0.7636379451959954
1107	11	0.7168446740694776
1107	12	0.936423090153724
1107	13	0.9186842419154289
1107	14	1.0976337317757725
1107	15	1.1107879685917155
1107	16	1.0975348391504482
1107	17	1.2316414712940187
1107	18	1.534729775607289
1107	19	1.3404129348556495
1107	20	1.1026815788397997
1107	21	1.1927703453279932
1107	22	1.2207497455297656
1107	23	1.4032140195717702
1107	24	1.3740457588224047
1107	25	1.3514834288918323
1108	1	0.544949788974467
1108	2	0.569040604829663
1108	3	0.5180325048116075
1108	4	0.5700787659742304
1108	5	0.5269052471563498
1108	6	0.6291736041140936
1108	7	0.6691679451267802
1108	8	0.6929056892671284
1108	9	0.8190045325593758
1108	10	0.7540978499540998
1108	11	0.6354326871631599
1108	12	0.6692897157363049
1108	13	0.5449298632166091
1108	14	0.8527805741226544
1108	15	0.837367127815007
1108	16	0.8262455472937447
1108	17	0.8919206642827788
1108	18	1.1325178050015339
1108	19	1.062802910171932
1108	20	1.026030855127793
1108	21	0.891522312207897
1108	22	0.7472281905219944
1108	23	0.976881364874373
1108	24	1.0376179162859032
1108	25	1.1640382146058812
1109	1	0.3508003685508143
1109	2	0.32315683638665504
1109	3	0.4747841845572083
1109	4	0.583958785977309
1109	5	0.41382855587050926
1109	6	0.3274652216278966
1109	7	0.5058977411637713
1109	8	0.5208157523537622
1109	9	0.6998572492166417
1109	10	0.7482778306348905
1109	11	0.3633478792439959
1109	12	0.6837721788502904
1109	13	0.6780355974321282
1109	14	0.4898083860937277
1109	15	0.8576971785920477
1109	16	0.6695338309613135
1109	17	0.5452364521817685
1109	18	-1.0878148102726044
1109	19	0.33476964437035406
1109	20	0.6124396616108427
1109	21	0.6406779515370289
1109	22	0.8018519787971172
1109	23	0.39328175125860554
1109	24	0.7710507307359933
1109	25	0.8491759922126931
1110	1	0.2325185563955472
1110	2	0.2051509194426977
1110	3	0.2722560747826641
1110	4	0.14802976356028807
1110	5	0.2801389830361032
1110	6	0.2820806739051065
1110	7	0.3665671195843032
1110	8	0.27592088645041457
1110	9	0.5573261573709942
1110	10	0.6844197815100941
1110	11	0.5302935519155696
1110	12	-0.03257600691343043
1110	13	0.25981846832990385
1110	14	0.35281933394180953
1110	15	0.43600711661216707
1110	16	0.5680117947431
1110	17	0.17190367664591122
1110	18	0.5939963795737597
1110	19	0.5944821855883688
1110	20	0.515519025464279
1110	21	0.4446149290041532
1110	22	0.5019060422444284
1110	23	0.4157208708825664
1110	24	0.6947381367417549
1110	25	0.6205526149206264
1111	1	0.27191156787334947
1111	2	0.4908573409560084
1111	3	0.4942539897863796
1111	4	0.2729364376127492
1111	5	0.4195215007207267
1111	6	0.5551407844167926
1111	7	0.4513707308253956
1111	8	0.34066630014670807
1111	9	0.5201759791485401
1111	10	0.4769845849837111
1111	11	0.4363895856058022
1111	12	0.4750242518187804
1111	13	0.5098515839887128
1111	14	0.4096152692312316
1111	15	0.6299066101576258
1111	16	0.48327011408470355
1111	17	0.5866213936093843
1111	18	0.6279081044093004
1111	19	0.44764950481987853
1111	20	0.3893883622565468
1111	21	0.5585100013941258
1111	22	0.29533701372884025
1111	23	0.7116333664097994
1111	24	0.7545480270558116
1111	25	0.29746248527927577
1112	1	-0.19066590217617474
1112	2	0.06795387417129106
1112	3	0.08060456251138959
1112	4	0.1774878894844818
1112	5	0.08238758128957357
1112	6	0.11124129143059491
1112	7	-0.19575319358597199
1112	8	0.1929903151703627
1112	9	0.3880588506589291
1112	10	0.40493095426683823
1112	11	0.24936512720445592
1112	12	0.37213088380837434
1112	13	0.127076478850789
1112	14	0.29597941650732906
1112	15	0.27353573612946036
1112	16	0.489855068294375
1112	17	0.49161207299405163
1112	18	0.3938141062630507
1112	19	0.37410180886215727
1112	20	0.1927497496621342
1112	21	0.33150243768884025
1112	22	0.22937463858012563
1112	23	0.39584996782639337
1112	24	0.09935502633644688
1112	25	0.2656634508881055
1113	1	-0.5604397930962932
1113	2	-0.3531926061807411
1113	3	-0.5024427047054765
1113	4	-0.20829591970829142
1113	5	-0.5440872887453239
1113	6	-0.33472927304176636
1113	7	-0.4496488734948798
1113	8	-0.13598695059786792
1113	9	-0.4045458193422675
1113	10	-0.558073707118588
1113	11	-0.4754868525856537
1113	12	-0.2812683756576746
1113	13	-0.2932240510613511
1113	14	-0.31545597091305666
1113	15	-0.6554370528166729
1113	16	-0.5428263665355573
1113	17	-0.548815319920152
1113	18	-0.5958763214888521
1113	19	-0.513823483957907
1113	20	-0.8357090071919444
1113	21	-0.609342165481003
1113	22	-0.33389668935934225
1113	23	-0.7045007447274187
1113	24	-0.1959158868634674
1113	25	-0.19599309576734925
1114	1	-1.0724139860328563
1114	2	-0.9209781090412434
1114	3	-0.7352923706512478
1114	4	-0.7568970125494374
1114	5	-0.9600674678866774
1114	6	-1.493395590590083
1114	7	-1.517985748365323
1114	8	-0.9647853120914576
1114	9	-0.8656659101801765
1114	10	-1.1374707520331668
1114	11	-1.7733937999568994
1114	12	-1.3088904768633896
1114	13	-1.2048686796321562
1114	14	-1.554170363568815
1114	15	-1.2943092620728436
1114	16	-2.054774858151262
1114	17	-4.121492885625319
1114	18	-2.9266140312221225
1114	19	-1.953249561923755
1114	20	-1.0748525594543779
1114	21	-2.25669853810777
1114	22	-2.2710827660831328
1114	23	-2.2401962077833915
1114	24	-1.7128524278894872
1114	25	-1.5811076670105706
1115	1	-1.1033146467661972
1115	2	-0.8901981144539839
1115	3	-0.8252440266217214
1115	4	-0.8356751988683087
1115	5	-0.7145372866978406
1115	6	-0.8017915410342198
1115	7	-1.8560718195273225
1115	8	-1.2720043366608067
1115	9	-0.868720918428572
1115	10	-0.625631032874999
1115	11	-1.4149776379332906
1115	12	-1.078094494011772
1115	13	-0.9527305186187007
1115	14	-0.9485674465509248
1115	15	-2.2928459695763834
1115	16	-1.1021130491588735
1115	17	-0.9739067687825695
1115	18	-0.9942187478893914
1115	19	-1.0728723767891353
1115	20	-0.8325213239881819
1115	21	-1.4835829985491031
1115	22	-1.6481694285092487
1115	23	-1.1703688638004124
1115	24	-1.1415997150559534
1115	25	-1.3742787819485187
1116	1	-1.0644346570657532
1116	2	-1.7917391562944722
1116	3	-1.3642990218124318
1116	4	-0.8897131892381402
1116	5	-1.4515528831954114
1116	6	-1.232855833145809
1116	7	-0.9421060088317219
1116	8	-1.8030162202683933
1116	9	-1.6836318622639155
1116	10	-2.6839540187025577
1116	11	-1.2486625001343228
1116	12	-1.1373322483363566
1116	13	-1.6263089534714368
1116	14	-1.36268280362939
1116	15	-1.0733626942435848
1116	16	-1.0098907443727896
1116	17	-1.4244044322367628
1116	18	-1.0770467455388797
1116	19	-1.4528224396814124
1116	20	-1.819227012773986
1116	21	-2.100444855155544
1116	22	-1.4967527326749988
1116	23	-1.371761683959555
1116	24	-1.8993474078502146
1116	25	-1.6575710667833548
1201	1	0.40018682696442553
1201	2	0.43541368182098594
1201	3	0.5589331471453001
1201	4	0.5748083248577771
1201	5	0.6803976039177044
1201	6	0.7023504055761975
1201	7	0.6006163455029494
1201	8	0.15047639920555866
1201	9	0.3810011567586784
1201	10	0.656981586668266
1201	11	0.2334260597699398
1201	12	-0.1471358170280368
1201	13	-0.10183784088403058
1201	14	0.6267005175192182
1201	15	0.32569111596200173
1201	16	0.786822316816604
1201	17	0.8703138763061276
1201	18	1.147906754022955
1201	19	0.8056173564696394
1201	20	0.8631790798381189
1201	21	0.7611239144933322
1201	22	1.0003928373271833
1201	23	1.3121498319680853
1201	24	0.926181559666496
1201	25	0.9052878597253908
1202	1	0.698560720195907
1202	2	0.7449300113741195
1202	3	0.36516398214899937
1202	4	0.7372448446780524
1202	5	0.6829839640648885
1202	6	0.7336178875791006
1202	7	0.7129961244027854
1202	8	0.6951105499539736
1202	9	1.01712113859692
1202	10	1.0472423151319248
1202	11	0.7595544522037798
1202	12	0.8782159213074365
1202	13	0.7360567008550802
1202	14	1.048431794618324
1202	15	0.737672513566352
1202	16	0.8829033756074267
1202	17	1.1467132088207421
1202	18	1.431243807913134
1202	19	1.1959104916634047
1202	20	1.047339999070175
1202	21	0.9706090613115563
1202	22	1.2100076110658406
1202	23	1.276369304799175
1202	24	1.333931664143229
1202	25	1.2551862389808832
1203	1	0.340583377109656
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2211	15	0.25202215822120166
2211	16	0.07009052375124725
2211	17	0.19082067046010565
2211	18	0.10891525445038042
2211	19	-0.06260676695958978
2211	20	-0.12316626349068116
2211	21	-0.052101824681749065
2211	22	-0.10701083098884112
2211	23	0.4822401991434617
2211	24	0.2585891814032024
2211	25	0.2395556518373425
2212	1	-0.11176486998354918
2212	2	-0.12360434517421126
2212	3	-0.15515406144715982
2212	4	-0.1860206727278566
2212	5	-0.007993357771482579
2212	6	-0.14838197802817632
2212	7	-0.27228565806542804
2212	8	-0.197479661716784
2212	9	-0.08068842602489212
2212	10	-0.027176162340190047
2212	11	-0.17278512035428406
2212	12	-0.03825623199946193
2212	13	-0.1061380098501914
2212	14	-0.07163936046280384
2212	15	-0.19631032556743833
2212	16	-0.05589579328315608
2212	17	-0.05833986911031985
2212	18	-2.8177620014172433
2212	19	-1.0193084992907657
2212	20	-0.33589613570203625
2212	21	-0.3529664449831529
2212	22	-0.06055549999070564
2212	23	-0.17093031470557207
2212	24	-0.5817645759711851
2212	25	-0.17919994438415188
2213	1	0.20137193818356636
2213	2	0.17313484764687104
2213	3	-0.1951162303942553
2213	4	-0.22331021254154848
2213	5	-0.052946430795891786
2213	6	-0.06963798894827278
2213	7	0.26446611183393287
2213	8	-0.2333817301969816
2213	9	-0.23158092444651857
2213	10	-0.24992520221502446
2213	11	-0.1840221408843732
2213	12	-0.20432357426817305
2213	13	0.011647326374543354
2213	14	-0.10800308757014676
2213	15	-0.3771607257131748
2213	16	-0.730473874729725
2213	17	-0.401187529249718
2213	18	-0.1686175671487007
2213	19	-0.11698298753594116
2213	20	-0.7816562379157546
2213	21	-0.5121513305292424
2213	22	-0.4760482644275186
2213	23	-0.8173671835121681
2213	24	-0.4864657580668137
2213	25	-0.5742799111447283
2214	1	-1.0998411063949014
2214	2	-3.1321149171843494
2214	3	-1.389992072280151
2214	4	-0.941054613874261
2214	5	-0.7846816555035616
2214	6	-1.021838100619064
2214	7	-1.0950322314409746
2214	8	-0.7250828623077368
2214	9	-0.985633574748249
2214	10	-0.9301929599792587
2214	11	-0.6936063001353503
2214	12	-1.1886497813303905
2214	13	-0.8990561537566748
2214	14	-2.1672203514341035
2214	15	-2.344408659620207
2214	16	-1.6877801974234643
2214	17	-2.1808476759803668
2214	18	-1.3740002504749143
2214	19	-3.429310278299905
2214	20	-1.8164109059931306
2214	21	-1.664826295185165
2214	22	-1.8991664099222731
2214	23	-1.8835731546904668
2214	24	-2.0721406557936106
2214	25	-1.8329448263464414
2215	1	-0.8855222342866007
2215	2	-2.7117188134697408
2215	3	-1.5344059524474822
2215	4	-0.9477570027015538
2215	5	-0.9304634747017566
2215	6	-1.573984807596414
2215	7	-1.9699327746103634
2215	8	-1.9041169198669259
2215	9	-1.5249590850759205
2215	10	-3.3750420511255044
2215	11	-2.2753188209816884
2215	12	-2.268609812262163
2215	13	-1.7174775618976952
2215	14	-3.231658478571145
2215	15	-2.864957675300378
2215	16	-2.5890093161474326
2215	17	-2.849160310491655
2215	18	-1.5641951346205119
2215	19	-2.1914586855930978
2215	20	-2.446674548116242
2215	21	-2.0216713474989874
2215	22	-1.7417688714205966
2215	23	-2.43340661690619
2215	24	-2.2294314004853604
2215	25	-2.147726510451051
2216	1	-1.8838454975958392
2216	2	-1.9533067418694685
2216	3	-2.5031552639290062
2216	4	-3.191760607007513
2216	5	-2.152300597358323
2216	6	-2.2836167220873627
2216	7	-2.145861886160244
2216	8	-2.703959867036726
2216	9	-3.0024369748056294
2216	10	-2.4727560285449215
2216	11	-3.1359446030680616
2216	12	-3.4493894835246017
2216	13	-4.10401395380665
2216	14	-3.390623624706542
2216	15	-3.285151392622229
2216	16	-2.387199254032474
2216	17	-2.483450931669495
2216	18	-2.5303208729241753
2216	19	-2.8913476752293015
2216	20	-2.6830927266988667
2216	21	-3.0072682471179384
2216	22	-3.6328888897245974
2216	23	-3.5679506128838305
2216	24	-3.5038094741024643
2216	25	-3.71534312076513
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89995.0
3	4.0
4	702.0
5	4347.0
6	5955.0
7	5142.0
8	4639.0
9	4307.0
10	3936.0
11	3805.0
12	3571.0
13	3606.0
14	3628.0
15	3586.0
16	3805.0
17	4003.0
18	4456.0
19	5356.0
20	6861.0
21	8745.0
22	11039.0
23	13393.0
24	15556.0
25	17825.0
26	20062.0
27	22126.0
28	25011.0
29	29734.0
30	36666.0
31	45875.0
32	57826.0
33	74032.0
34	95717.0
35	125169.0
36	171368.0
37	264601.0
38	728398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63901222312362	18.393652985082408	12.380318289221503	25.587016502572467
2	14.811713741479629	19.712743592342257	41.245436094934426	24.230106571243695
3	18.985414903989074	24.108128446965836	29.262492230359697	27.643964418685385
4	12.127738415540152	16.469913224066094	38.020924553218535	33.38142380717522
5	12.952031092940992	37.98152635239013	36.07828951403247	12.98815304063641
6	32.56805221987469	36.74497707949896	17.926159717076526	12.760810983549826
7	28.557146509312588	31.196128640701026	22.090504922857566	18.156219927128824
8	25.21618887120335	37.073331951054875	20.343137637000165	17.367341540741617
9	26.43546699119634	15.006084931298997	19.87574476753068	38.68270330997399
10	14.531507623780229	29.104793656748797	34.95179634168711	21.411902377783864
11	34.491585136963664	22.368705480832986	24.42066624680688	18.719043135396465
12	23.349421080144623	24.739899170982195	31.661007895259207	20.249671853613975
13	28.91690725463664	21.800491776360232	25.644962825781054	23.63763814322207
14	20.603348552767926	22.245873031875586	27.634706546920583	29.516071868435905
15	23.33581392254949	30.858599139207826	22.969026240545784	22.836560697696896
16	22.0600080024527	28.2441891280964	27.848535395264	21.847267474186893
17	21.930214297926028	28.72264634216684	27.755528152747615	21.59161120715951
18	21.851666926717638	27.45888594164456	29.5289436729599	21.1605034586779
19	23.33057221637318	27.388063036611992	27.60943740769572	21.6719273393191
20	23.527082517052264	27.44102250181617	27.938170151602232	21.093724829529332
21	22.691704082481085	27.027105678889168	27.35496175272304	22.926228485906712
22	22.92402048789968	28.21617363479389	26.889458978733177	21.970346898573258
23	21.93260603641889	28.004256942214294	27.692988828644566	22.37014819272225
24	22.87046010189845	27.26705863707576	28.132961628296695	21.729519632729097
25	22.571610002587843	27.346493575599855	27.58485581345076	22.49704060836154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	132.0
1	132.0
2	295.5
3	459.0
4	459.0
5	459.0
6	881.0
7	1303.0
8	1303.0
9	1303.0
10	1941.0
11	2579.0
12	2579.0
13	2579.0
14	4874.5
15	7170.0
16	7170.0
17	7170.0
18	13784.5
19	20399.0
20	20399.0
21	20399.0
22	32743.0
23	45087.0
24	45087.0
25	45087.0
26	65099.5
27	85112.0
28	85112.0
29	85112.0
30	111008.0
31	136904.0
32	136904.0
33	136904.0
34	158781.0
35	180658.0
36	180658.0
37	180658.0
38	198767.0
39	216876.0
40	216876.0
41	216876.0
42	228693.5
43	240511.0
44	240511.0
45	240511.0
46	254417.0
47	268323.0
48	268323.0
49	268323.0
50	255528.0
51	242733.0
52	242733.0
53	242733.0
54	214047.5
55	185362.0
56	185362.0
57	185362.0
58	158897.5
59	132433.0
60	132433.0
61	132433.0
62	107321.5
63	82210.0
64	82210.0
65	82210.0
66	63211.0
67	44212.0
68	44212.0
69	44212.0
70	32336.0
71	20460.0
72	20460.0
73	20460.0
74	14105.5
75	7751.0
76	7751.0
77	7751.0
78	5322.0
79	2893.0
80	2893.0
81	2893.0
82	1886.5
83	880.0
84	880.0
85	880.0
86	587.5
87	295.0
88	295.0
89	295.0
90	188.0
91	81.0
92	81.0
93	81.0
94	47.5
95	14.0
96	14.0
97	14.0
98	12.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3555087755026763
2	0.040626605647098184
3	0.03589895716386809
4	0.036158718069540075
5	0.042029314537726896
6	0.019170354838592366
7	0.01844302430271081
8	0.025352664393585567
9	0.02187186825758099
10	0.021196489902833834
11	0.02052111154808668
12	0.020832824634893058
13	0.02052111154808668
14	0.028729556167321348
15	0.029768599790009287
16	0.02270310315573134
17	0.024417525133166425
18	0.11154133289554963
19	0.02623585147287031
20	0.02436557295203203
21	0.02737879945782704
22	0.02046915936695228
23	0.02394995550295686
24	0.019845733193339522
25	0.024157764227494445
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1924847.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.6665218135305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.26048068154212	60.50249535650315
2	11.603438205356566	16.86362990922992
3	2.8869278265334324	6.293490116426395
4	0.9816553747346813	2.853339268061087
5	0.45011276924038857	1.6354064682277656
6	0.22740826346190698	0.9914980522459051
7	0.13767895768335922	0.7003255687233376
8	0.09120751380623984	0.5302186233247209
9	0.057210649244861404	0.3741569002186167
>10	0.27291926913470793	3.4154966032624428
>50	0.015267479166017046	0.7512247302530641
>100	0.0123262850187636	1.9049604075315005
>500	0.001862443659620953	0.9038970244000286
>1k	0.0014326489689391686	2.004137243504141
>5k	7.163244844695842E-5	0.27572372808792883
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5297	0.27519070346889907	No Hit
CCCATGTACTCTGCGTTGATACCAC	4678	0.24303230334670758	No Hit
GTCCTACAGTGGACATTTCTAAATT	3485	0.18105335125337235	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3407	0.1770010811248894	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2849	0.1480117640518961	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2535	0.13169877917569553	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2358	0.1225032431149073	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2257	0.11725607282033325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.195218113439665E-5
2	0.0	0.0	0.0	0.0	5.195218113439665E-5
3	0.0	0.0	0.0	0.0	5.195218113439665E-5
4	0.0	0.0	0.0	0.0	5.195218113439665E-5
5	0.0	0.0	0.0	0.0	5.195218113439665E-5
6	0.0	0.0	0.0	0.0	5.195218113439665E-5
7	0.0	0.0	0.0	0.0	5.195218113439665E-5
8	0.0	0.0	0.0	5.195218113439665E-5	5.195218113439665E-5
9	0.0	0.0	0.0	5.195218113439665E-5	5.195218113439665E-5
10	0.0	0.0	0.0	5.195218113439665E-5	5.195218113439665E-5
11	0.0	0.0	0.0	5.195218113439665E-5	5.195218113439665E-5
12	0.0	0.0	0.0	5.195218113439665E-5	5.195218113439665E-5
13	0.0	0.0	0.0	1.039043622687933E-4	5.195218113439665E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTC	25	0.00605087	18.99005	8
TAGGACC	475	0.0	13.395766	4
GACCGTC	50	0.0015062958	13.292343	7
GTGCGCC	135	2.7284841E-11	12.660032	11
CGTGCGC	100	1.4529905E-7	12.343532	10
AGACCGT	55	0.0030821667	12.083948	6
GCGCCAC	115	7.046219E-8	11.568778	13
GGACGTG	1755	0.0	11.360977	6
AGGACGT	1790	0.0	11.353658	5
GGCGAGG	800	0.0	11.156074	19
GTCCTAA	850	0.0	11.098996	1
TTAGGAC	1015	0.0	11.040845	3
GACGTGA	905	0.0	11.015754	7
GTCCTAC	1600	0.0	10.958858	1
GTAGGAC	1720	0.0	10.9325905	3
TAGGACG	1915	0.0	10.860798	4
GGACCGT	70	0.0014988648	10.850892	6
AGGACCG	115	8.857696E-7	10.735457	5
ACGTGAA	985	0.0	10.699976	8
GTACCGT	80	3.7916747E-4	10.681347	6
>>END_MODULE