FastQCFastQC Report
Thu 26 May 2016
SRR1513001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924847
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC52750.2740477554839424No Hit
GTACATGGGAAGCAGTGGTATCAAC51750.2688525373705027No Hit
CTGTAGGACGTGGAATATGGCAAGA33910.17616984622673906No Hit
GTCCTACAGTGGACATTTCTAAATT33360.17331247626434723No Hit
GTATCAACGCAGAGTACTTTTTTTT31530.16380522711675266No Hit
GTCCTAAAGTGTGTATTTCTCATTT26380.13704985383253837No Hit
CTTTAGGACGTGAAATATGGCGAGG23350.12130834294881618No Hit
TATCAACGCAGAGTACTTTTTTTTT22620.11751583372600523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5400.013.395444
TACGCTG500.001478749113.3249385
ACCGTGC655.4352648E-513.1586198
GGACCGT550.003035607312.108536
CGGCTAG550.003068334712.09120710
TGGACCG951.0169151E-612.02255
GCGTGCG658.012784E-411.6944219
ACCGTCC752.0673232E-411.4041388
GTCCGTT752.0929449E-411.38902411
GTATACC600.005795141711.1041154
CGTGCGC700.001491373810.8574110
CTAGACT2300.010.7592674
CTAGAGC1951.8189894E-1210.7288193
GTCCTAC15100.010.7062111
CTTAGAC2050.010.6693463
CCGTCCA909.499425E-510.5574649
TAGACTG3250.010.5428085
AGGACCT11300.010.44432455
TCGAACT1553.7362042E-910.40688819
GTAGGAC16550.010.3428053
Produced by FastQC (version 0.11.5)