FastQCFastQC Report
Thu 26 May 2016
SRR1513000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265096
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC57490.45443191662925186No Hit
CCCATGTACTCTGCGTTGATACCAC47960.3791016650119833No Hit
GAGTACATGGGAAGCAGTGGTATCA24130.19073651327646282No Hit
GTATCAACGCAGAGTACTTTTTTTT22890.1809348855739011No Hit
GTCCTACAGTGGACATTTCTAAATT18680.14765677861601018No Hit
GTCCTAAAGTGTGTATTTCTCATTT17590.13904083168391965No Hit
CATGTACTCTGCGTTGATACCACTG16700.1320057924457907No Hit
CTGTAGGACGTGGAATATGGCAAGA15940.12599834320873673No Hit
CTTTAGGACGTGAAATATGGCGAGG15700.12410125397598284No Hit
GCGTTGATACCACTGCTTCCCATGT15680.12394316320658669No Hit
GTATCAACGCAGAGTACATGGGAAG15070.11912139474000392No Hit
TATCAACGCAGAGTACTTTTTTTTT14110.11153303780898842No Hit
GGTATCAACGCAGAGTACTTTTTTT13810.10916167626804606No Hit
TATCAACGCAGAGTACATGGGAAGC13140.10386563549327481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA1301.2732926E-1113.197841
TCTTAGC655.4706506E-513.1487812
GTACTAA1155.0622475E-912.4327481
TAGGACC2800.012.2095824
TAAGCTC701.0945178E-412.2095825
TCAATAC1251.8402716E-811.395613
GGCGAGG4500.011.1823719
GTCTTAG1657.4578566E-1110.9759821
AGGACGT9300.010.8237885
GGACGTG9500.010.7937086
GTCTAAA1158.438801E-710.7750481
GTCCTAA5100.010.6531581
TGTAGGA9700.010.5732472
GTATAAT1909.094947E-1210.5351181
GACGTGG5400.010.3735827
GGACCTG6450.010.30406956
GCTATAC1201.5299192E-610.28770353
TTAGGAC6050.010.2026823
GTCCTAC8100.010.0024781
TTGGACC951.6496702E-49.996154
Produced by FastQC (version 0.11.5)