##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513000_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1265096 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.991107394221466 31.0 30.0 34.0 23.0 34.0 2 29.1488898866173 31.0 30.0 34.0 22.0 34.0 3 29.310611210532638 31.0 30.0 34.0 23.0 34.0 4 32.73234995605077 35.0 33.0 37.0 25.0 37.0 5 32.848140378279595 35.0 35.0 37.0 26.0 37.0 6 32.88829464325237 37.0 35.0 37.0 26.0 37.0 7 32.73184643695024 37.0 35.0 37.0 25.0 37.0 8 32.71440507281661 37.0 35.0 37.0 25.0 37.0 9 34.01056125384951 39.0 35.0 39.0 23.0 39.0 10 33.910082713090546 39.0 34.0 39.0 23.0 39.0 11 33.957721785540386 39.0 35.0 39.0 23.0 39.0 12 33.852178806983815 39.0 34.0 39.0 23.0 39.0 13 33.83703054946028 39.0 34.0 39.0 22.0 39.0 14 34.85843445872882 39.0 35.0 41.0 22.0 41.0 15 34.784025876297136 39.0 34.0 41.0 19.0 41.0 16 34.66832556580686 39.0 34.0 41.0 18.0 41.0 17 34.62070941651859 39.0 34.0 41.0 18.0 41.0 18 34.32444731467019 39.0 34.0 41.0 17.0 41.0 19 34.54726914004945 39.0 34.0 41.0 18.0 41.0 20 34.63565847967269 39.0 34.0 41.0 18.0 41.0 21 34.57181115109051 39.0 34.0 41.0 18.0 41.0 22 34.493443185339295 39.0 34.0 41.0 18.0 41.0 23 34.22638756268299 39.0 34.0 41.0 11.0 41.0 24 34.23178636245787 39.0 34.0 41.0 10.0 41.0 25 34.151503917489265 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5808348926269709 1101 2 0.8378229166456563 1101 3 0.9283947221093172 1101 4 0.8303211448477583 1101 5 0.6432814892995964 1101 6 0.7525603689980329 1101 7 0.7432637267711186 1101 8 0.5926300829504001 1101 9 0.5675999332183679 1101 10 0.5167718710946616 1101 11 0.7957546603417853 1101 12 0.9857354277316688 1101 13 0.771687326754467 1101 14 0.6713884336805833 1101 15 0.659539299261553 1101 16 1.0611216711347282 1101 17 1.220173821624222 1101 18 1.3064883123297761 1101 19 1.015526929394447 1101 20 1.1615090603150904 1101 21 1.1574394310962859 1101 22 1.134355935421432 1101 23 1.3432810979944136 1101 24 1.0332546936935785 1101 25 1.2106489529751556 1102 1 0.6578000568741906 1102 2 0.824404944957621 1102 3 0.7440120942479744 1102 4 0.9915825311471025 1102 5 0.8604179858224512 1102 6 0.7714413819101438 1102 7 1.0175236328965696 1102 8 0.8772223951881415 1102 9 0.8725631283328781 1102 10 1.0230921831639677 1102 11 0.8999613405995674 1102 12 0.9010031596913777 1102 13 0.9475620712977317 1102 14 0.9431791670661624 1102 15 1.013504201160906 1102 16 1.1032768464382343 1102 17 0.8246238731657414 1102 18 1.139630633141607 1102 19 1.0358077773626135 1102 20 1.0728695542715343 1102 21 0.8570976585831929 1102 22 1.0732103201630352 1102 23 1.414180679706078 1102 24 1.2607729077555945 1102 25 1.137595459219007 1103 1 0.42437235912413485 1103 2 0.5460758887160537 1103 3 0.5110425329508317 1103 4 0.5538392624179167 1103 5 0.33112502270801514 1103 6 0.6218119967616857 1103 7 0.5422583508968444 1103 8 0.36870926854237496 1103 9 0.7856508697015627 1103 10 0.5487649647830182 1103 11 0.637460579917672 1103 12 0.7446497451530902 1103 13 0.702707536265045 1103 14 0.9237306900111193 1103 15 0.8868932496269082 1103 16 0.9030640415243738 1103 17 0.7923241902737672 1103 18 1.0984140310631432 1103 19 1.1250572228288291 1103 20 0.9213940705509245 1103 21 0.8465645062722231 1103 22 1.0284362513240524 1103 23 1.4059876373744302 1103 24 1.305889515972659 1103 25 1.0744605887900462 1104 1 0.4933775927726778 1104 2 0.5144439014273061 1104 3 0.5122720439086379 1104 4 0.9104399353970223 1104 5 0.49204585256702416 1104 6 0.4750063154623305 1104 7 0.7396548452488645 1104 8 0.6473211156901968 1104 9 0.7075975236956751 1104 10 0.8115048489783732 1104 11 0.7479183414873773 1104 12 0.6170436094658669 1104 13 0.7888108753006833 1104 14 0.9174074537828076 1104 15 0.9112404168802968 1104 16 0.8502927575107151 1104 17 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0.7481506298686185 1107 17 0.8984567715352227 1107 18 1.1754286588911569 1107 19 0.9602658447669796 1107 20 1.0220754851904914 1107 21 0.8040382330436842 1107 22 0.9017844786100824 1107 23 0.9498403954269605 1107 24 0.9561926497977566 1107 25 0.9386524714604079 1108 1 0.49440641276175157 1108 2 0.48800879025160526 1108 3 0.4057804052634921 1108 4 0.5175755261541823 1108 5 0.3857742100130608 1108 6 0.5064527271206174 1108 7 0.7139268285712603 1108 8 0.6290228531080757 1108 9 0.7655617329876279 1108 10 0.6598689099358381 1108 11 0.571896913166519 1108 12 0.765415688504099 1108 13 0.739440545206314 1108 14 0.9230887651399158 1108 15 0.8698255263020442 1108 16 0.7818589392505402 1108 17 0.8760622608353117 1108 18 1.141198148065122 1108 19 1.103372809959211 1108 20 0.8391456270556219 1108 21 0.8490503967233991 1108 22 0.7206965144680737 1108 23 0.9755388671448912 1108 24 1.0843616946275247 1108 25 1.1544565234331614 1109 1 0.19345695902449478 1109 2 0.056440008507561146 1109 3 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-2.2166506808393507 2215 17 -2.4083890038169145 2215 18 -1.1415248380147958 2215 19 -2.0394868423774923 2215 20 -2.051097014522945 2215 21 -1.673048331221409 2215 22 -1.313896912201166 2215 23 -1.7748580070473707 2215 24 -1.7343550077155712 2215 25 -1.4935475619995628 2216 1 -2.5168363258171667 2216 2 -2.4029565384780724 2216 3 -3.1343760594476144 2216 4 -3.8389487149622035 2216 5 -3.063551364575563 2216 6 -2.9977347130393177 2216 7 -2.855043649504335 2216 8 -3.5184558029027215 2216 9 -3.7172722923820807 2216 10 -3.211125588499023 2216 11 -3.8434593491425524 2216 12 -4.277185475307608 2216 13 -4.603029156498721 2216 14 -4.0952226661103595 2216 15 -3.970788823407002 2216 16 -3.257260215026445 2216 17 -3.31943776644928 2216 18 -3.333613216213898 2216 19 -3.729652951652863 2216 20 -3.813595481889262 2216 21 -4.001167631940302 2216 22 -4.5515818478613355 2216 23 -4.464784281595804 2216 24 -4.486787085751381 2216 25 -4.660189347416484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61285.0 3 1.0 4 522.0 5 3056.0 6 4131.0 7 3522.0 8 3345.0 9 2983.0 10 2665.0 11 2560.0 12 2416.0 13 2432.0 14 2479.0 15 2456.0 16 2479.0 17 2547.0 18 2955.0 19 3592.0 20 4591.0 21 5965.0 22 7116.0 23 8703.0 24 10242.0 25 11660.0 26 12696.0 27 14216.0 28 16043.0 29 19224.0 30 23160.0 31 29521.0 32 36562.0 33 47155.0 34 61332.0 35 80743.0 36 110469.0 37 171694.0 38 488578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72509652019606 18.481505485012498 12.188872591754672 25.604525403036764 2 14.678621952694963 20.33669117769162 40.87874357208321 24.105943297530207 3 18.7604129848092 24.925848873242888 29.55678541126496 26.756952730682947 4 12.15872142305892 16.92333725534849 38.21711200516188 32.70082931643071 5 12.816522510639711 38.20545910887378 36.12488356020313 12.853134820283376 6 31.76469193095079 36.85760478142455 18.423742682198917 12.953960605425745 7 27.55416659419683 31.738469784819912 22.734869483134553 17.972494137848706 8 25.10586486614696 36.64466090036527 20.88392024161541 17.36555399187236 9 26.62168145091156 14.9913033853549 20.113135050519425 38.27388011321411 10 14.751297569305816 29.02349262569523 35.02895565929961 21.196254145699346 11 33.87565134688772 22.656870736252245 24.561954147392754 18.905523769467276 12 23.292172220478516 25.12295759158868 31.670365342759354 19.914504845173443 13 28.86582452212329 22.034587062977423 25.506101041533253 23.59348737336603 14 20.799483224973454 22.425632547338758 27.681857606974248 29.09302662071354 15 23.601794484979017 30.353488872232294 23.31075204544089 22.733964597347807 16 21.877562056618437 28.090378881972843 27.987125776671277 22.044933284737436 17 22.032552914100123 28.59223312484731 27.766511468312416 21.608702492740147 18 21.996876048438658 27.29437941926785 29.507574079114786 21.201170453178708 19 23.160821755781924 27.59028276317912 27.7921322196957 21.45676326134326 20 23.493085269552733 27.42885243620517 27.84147510032921 21.236587193912886 21 22.553765800041273 27.180142806202763 27.60282133569894 22.663270058057023 22 22.90907365974369 28.201159012388626 26.74266920711219 22.147098120755494 23 21.803091144406523 28.183507940536305 27.633319339457437 22.38008157559974 24 22.680369173944047 27.67602883440142 27.93091507639472 21.712686915259813 25 22.448155088035545 27.24895835804021 27.454361455689696 22.848525098234546 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 178.0 1 178.0 2 311.5 3 445.0 4 445.0 5 445.0 6 923.5 7 1402.0 8 1402.0 9 1402.0 10 1961.0 11 2520.0 12 2520.0 13 2520.0 14 4298.0 15 6076.0 16 6076.0 17 6076.0 18 10628.0 19 15180.0 20 15180.0 21 15180.0 22 23627.5 23 32075.0 24 32075.0 25 32075.0 26 45009.0 27 57943.0 28 57943.0 29 57943.0 30 74249.5 31 90556.0 32 90556.0 33 90556.0 34 103894.5 35 117233.0 36 117233.0 37 117233.0 38 127702.5 39 138172.0 40 138172.0 41 138172.0 42 146356.5 43 154541.0 44 154541.0 45 154541.0 46 167144.5 47 179748.0 48 179748.0 49 179748.0 50 170510.0 51 161272.0 52 161272.0 53 161272.0 54 141285.5 55 121299.0 56 121299.0 57 121299.0 58 103133.0 59 84967.0 60 84967.0 61 84967.0 62 69000.0 63 53033.0 64 53033.0 65 53033.0 66 40699.5 67 28366.0 68 28366.0 69 28366.0 70 20566.5 71 12767.0 72 12767.0 73 12767.0 74 8843.0 75 4919.0 76 4919.0 77 4919.0 78 3291.0 79 1663.0 80 1663.0 81 1663.0 82 1095.0 83 527.0 84 527.0 85 527.0 86 338.5 87 150.0 88 150.0 89 150.0 90 98.5 91 47.0 92 47.0 93 47.0 94 30.0 95 13.0 96 13.0 97 13.0 98 8.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35404427806269245 2 0.039127465425548735 3 0.03549137772943713 4 0.03477996926715443 5 0.03904842004085066 6 0.01588812232431373 7 0.015967167709011805 8 0.0218165261766696 9 0.018654710788746465 10 0.018259483865256075 11 0.01715284847948298 12 0.018101393095859918 13 0.018180438480557996 14 0.026005931565667745 15 0.026243067719761978 16 0.019208028481633017 17 0.020788936175594578 18 0.10370754472387865 19 0.023081252331838848 20 0.020551800021500345 21 0.024108842332913865 22 0.017864256941765685 23 0.02031466386740611 24 0.017231893864181058 25 0.021026072329688814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1265096.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.64414940639169 #Duplication Level Percentage of deduplicated Percentage of total 1 83.84965363017805 61.75036419615025 2 11.441684267517374 16.852262113156215 3 2.7848911250585884 6.152728142830467 4 0.8977847505423859 2.6446637721489425 5 0.38112565974817597 1.4033837514552137 6 0.18771478560924934 0.8294457430317805 7 0.10992138738801825 0.5666546953032753 8 0.06858138158783367 0.4040494009720946 9 0.04644538309327829 0.3078387658582636 >10 0.1997241826083429 2.6292388456984064 >50 0.0162077496876815 0.8234420432514507 >100 0.01249552531675739 2.0207527414118656 >500 0.0020466646177216353 0.990817730212854 >1k 0.0016157878560960279 2.1682963186475535 >5k 1.0771919040640184E-4 0.4560617398713929 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 5749 0.45443191662925186 No Hit CCCATGTACTCTGCGTTGATACCAC 4796 0.3791016650119833 No Hit GAGTACATGGGAAGCAGTGGTATCA 2413 0.19073651327646282 No Hit GTATCAACGCAGAGTACTTTTTTTT 2289 0.1809348855739011 No Hit GTCCTACAGTGGACATTTCTAAATT 1868 0.14765677861601018 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1759 0.13904083168391965 No Hit CATGTACTCTGCGTTGATACCACTG 1670 0.1320057924457907 No Hit CTGTAGGACGTGGAATATGGCAAGA 1594 0.12599834320873673 No Hit CTTTAGGACGTGAAATATGGCGAGG 1570 0.12410125397598284 No Hit GCGTTGATACCACTGCTTCCCATGT 1568 0.12394316320658669 No Hit GTATCAACGCAGAGTACATGGGAAG 1507 0.11912139474000392 No Hit TATCAACGCAGAGTACTTTTTTTTT 1411 0.11153303780898842 No Hit GGTATCAACGCAGAGTACTTTTTTT 1381 0.10916167626804606 No Hit TATCAACGCAGAGTACATGGGAAGC 1314 0.10386563549327481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAA 130 1.2732926E-11 13.19784 1 TCTTAGC 65 5.4706506E-5 13.148781 2 GTACTAA 115 5.0622475E-9 12.432748 1 TAGGACC 280 0.0 12.209582 4 TAAGCTC 70 1.0945178E-4 12.209582 5 TCAATAC 125 1.8402716E-8 11.39561 3 GGCGAGG 450 0.0 11.18237 19 GTCTTAG 165 7.4578566E-11 10.975982 1 AGGACGT 930 0.0 10.823788 5 GGACGTG 950 0.0 10.793708 6 GTCTAAA 115 8.438801E-7 10.775048 1 GTCCTAA 510 0.0 10.653158 1 TGTAGGA 970 0.0 10.573247 2 GTATAAT 190 9.094947E-12 10.535118 1 GACGTGG 540 0.0 10.373582 7 GGACCTG 645 0.0 10.3040695 6 GCTATAC 120 1.5299192E-6 10.2877035 3 TTAGGAC 605 0.0 10.202682 3 GTCCTAC 810 0.0 10.002478 1 TTGGACC 95 1.6496702E-4 9.99615 4 >>END_MODULE