FastQCFastQC Report
Thu 26 May 2016
SRR1513000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265096
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC57630.45553855201502497No Hit
CCCATGTACTCTGCGTTGATACCAC54210.4285050304482822No Hit
GTATCAACGCAGAGTACTTTTTTTT26990.2133434933001132No Hit
GAGTACATGGGAAGCAGTGGTATCA21350.16876189633039707No Hit
TATCAACGCAGAGTACTTTTTTTTT20610.16291253786273927No Hit
GTCCTACAGTGGACATTTCTAAATT17340.13706469706646768No Hit
CATGTACTCTGCGTTGATACCACTG17280.1365904247582792No Hit
GGTATCAACGCAGAGTACTTTTTTT16790.13271720090807337No Hit
GTCCTAAAGTGTGTATTTCTCATTT16380.12947634013545217No Hit
CTGTAGGACGTGGAATATGGCAAGA16380.12947634013545217No Hit
CTTTAGGACGTGAAATATGGCGAGG16360.12931824936605602No Hit
GCGTTGATACCACTGCTTCCCATGT14500.11461580781221346No Hit
TATCAACGCAGAGTACATGGGAAGC14220.11240253704066726No Hit
GTATCAACGCAGAGTACATGGGAAG14140.11177017396308264No Hit
ACGCAGAGTACATGGGAAGCAGTGG13580.10734363241999027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAG853.8989238E-612.3061111
ATAATAC907.383347E-611.6215183
TATACTG2000.010.9421595
CGTGCAC700.001490757510.85741410
GGCGAGG4850.010.76196819
GGCGTGC1158.762072E-710.74364958
GTGCTAG1355.6001227E-810.5658531
ATGTACC1201.4920242E-610.3078314
AAAGGCG1201.4920242E-610.3078315
GCTGGAC1201.5049754E-610.3008923
AAGGCGT1303.7699465E-710.2439976
CTATACT1502.3825123E-810.1492484
TAAGTAG750.00261274410.1492485
TTAGGAC6400.010.10279753
AGTCGTC856.5656786E-410.0630568
AGGACGT9650.010.0572225
GTTCTAG951.6271784E-410.0097561
CTAGTTC951.6284798E-410.0089633
GTCCTAT1151.0138334E-59.9227141
GACAGTC1151.020536E-59.9172157
Produced by FastQC (version 0.11.5)