FastQCFastQC Report
Thu 26 May 2016
SRR1512999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183262
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22650.19141998982473873No Hit
GTACATGGGAAGCAGTGGTATCAAC17530.1481497757893011No Hit
GTCCTACAGTGGACATTTCTAAATT16070.13581100381825834No Hit
CCCATGTACTCTGCGTTGATACCAC15740.1330221033042555No Hit
GTCCTAAAGTGTGTATTTCTCATTT15620.13200795766279996No Hit
CTGTAGGACGTGGAATATGGCAAGA15080.1274443022762499No Hit
GGTATCAACGCAGAGTACTTTTTTT14360.12135942842751647No Hit
TATCAACGCAGAGTACTTTTTTTTT14050.11873955218708958No Hit
CTTTAGGACGTGAAATATGGCGAGG12740.10766846226786628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG1302.0008883E-1012.41597519
TAGGACC2900.011.7913464
GGCGAGG2750.011.7387419
GTCCTAC8750.011.54297351
CTACACT1657.2759576E-1211.5134344
GGACAGT1751.8189894E-1211.3934826
AGGACCT5100.011.1748045
GTCCTAA4900.011.0840541
TAGAAGT1408.356437E-910.8555244
GGGTATA803.6666766E-410.7194471
GGACTGT1701.4006218E-1010.6115766
CTTATAC909.5241274E-510.5539823
GACGTGA4500.010.5495217
TCCTACA10100.010.5330832
TACACTA1106.050859E-610.3620915
GTATTAT1303.6824713E-710.2613521
GAATAAC750.002582025710.1636241
GATATAC2750.010.0481281
CCTACAG9750.010.0344013
CGTGAAA5500.09.8406579
Produced by FastQC (version 0.11.5)