##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512999_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1183262 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.957566456118762 31.0 30.0 34.0 23.0 34.0 2 29.123657313426783 31.0 30.0 34.0 22.0 34.0 3 29.278847795331888 31.0 30.0 34.0 23.0 34.0 4 32.703781580072715 35.0 33.0 37.0 25.0 37.0 5 32.80269373984798 35.0 35.0 37.0 26.0 37.0 6 32.86029636716129 37.0 35.0 37.0 26.0 37.0 7 32.69609858171732 37.0 35.0 37.0 25.0 37.0 8 32.69733752964263 37.0 35.0 37.0 25.0 37.0 9 33.992971125583345 39.0 35.0 39.0 23.0 39.0 10 33.88078210911869 39.0 34.0 39.0 23.0 39.0 11 33.92414359626186 39.0 35.0 39.0 23.0 39.0 12 33.828512197636705 39.0 34.0 39.0 22.0 39.0 13 33.816486120571774 39.0 34.0 39.0 22.0 39.0 14 34.82467112101969 39.0 35.0 41.0 22.0 41.0 15 34.75542694686384 39.0 34.0 41.0 19.0 41.0 16 34.654328458109866 39.0 34.0 41.0 18.0 41.0 17 34.59658638577086 39.0 34.0 41.0 18.0 41.0 18 34.30754473649961 39.0 34.0 41.0 17.0 41.0 19 34.536041045854596 39.0 34.0 41.0 18.0 41.0 20 34.62007653419108 39.0 34.0 41.0 18.0 41.0 21 34.56050392896924 39.0 34.0 41.0 18.0 41.0 22 34.477340605884415 39.0 34.0 41.0 18.0 41.0 23 34.21944421438363 39.0 34.0 41.0 10.0 41.0 24 34.215805121773535 39.0 34.0 41.0 10.0 41.0 25 34.13515687987952 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6784939452468848 1101 2 0.9567596248123209 1101 3 0.9860734115319332 1101 4 1.0312932322589958 1101 5 0.8110168535852935 1101 6 0.9381640077803581 1101 7 1.1097389534819584 1101 8 0.8444971454803962 1101 9 0.8949551327299332 1101 10 0.8244300887793372 1101 11 1.0356771483734306 1101 12 1.1405708389197144 1101 13 0.9960441274986778 1101 14 1.0665014473872532 1101 15 0.9658332952142104 1101 16 1.2193697194977489 1101 17 1.1444702211278113 1101 18 1.45822586573874 1101 19 1.0145043559219928 1101 20 1.0703735073686218 1101 21 1.071397987576944 1101 22 1.0746058286268365 1101 23 1.3628597690911093 1101 24 0.9543494807308619 1101 25 1.1022926880135628 1102 1 0.6703657442840054 1102 2 0.7119560412941333 1102 3 0.64943238469073 1102 4 0.8690886783983842 1102 5 0.8140452348315392 1102 6 0.6627217353227195 1102 7 0.7823439885061276 1102 8 0.8418976752865035 1102 9 0.7788627999891844 1102 10 0.9573236812092105 1102 11 0.8752995965132797 1102 12 0.9542270364243848 1102 13 0.8081988303921079 1102 14 0.8098407065458915 1102 15 1.065111213361945 1102 16 1.15394885282209 1102 17 0.9300728212710467 1102 18 1.1808088280578914 1102 19 1.1962402709205122 1102 20 1.1570159873678136 1102 21 0.920114145439463 1102 22 1.1886481378570224 1102 23 1.427094552920856 1102 24 1.2478503769469356 1102 25 1.1728926097571772 1103 1 0.7429991778372909 1103 2 0.9786349576146733 1103 3 0.8447771210687947 1103 4 1.063469050896522 1103 5 0.6338911559190734 1103 6 0.8961715885491444 1103 7 0.815281134445442 1103 8 0.9011438177568962 1103 9 1.0714811486864448 1103 10 1.105158826552838 1103 11 1.1108020570637152 1103 12 1.2892196051323452 1103 13 1.1800663148219641 1103 14 1.400335765440552 1103 15 1.318051352563458 1103 16 1.4193199210166014 1103 17 1.242501231182004 1103 18 1.500714169845466 1103 19 1.6219903275998533 1103 20 1.432661057598338 1103 21 1.3265132065627654 1103 22 1.448477225549233 1103 23 1.6296791311005663 1103 24 1.5364355271064483 1103 25 1.445650515599823 1104 1 0.33968319048646833 1104 2 0.5949214287632607 1104 3 0.4061501457219343 1104 4 0.8116955703857442 1104 5 0.5194508792146451 1104 6 0.6993301136234962 1104 7 0.8367928592953362 1104 8 0.7185945817969142 1104 9 0.6641697373111768 1104 10 0.6264613266652077 1104 11 0.7616525128488547 1104 12 0.44392353690449227 1104 13 0.6115065726773139 1104 14 0.7296711615393718 1104 15 0.7595729659283208 1104 16 0.632200799209528 1104 17 0.6565414725081666 1104 18 0.8858619683459494 1104 19 0.9369409792703891 1104 20 0.9264389777913564 1104 21 1.0476440350956224 1104 22 0.9263703048936875 1104 23 1.186818653407002 1104 24 0.9775888585355403 1104 25 0.6139098324803243 1105 1 0.7511421768442403 1105 2 1.030962542074473 1105 3 0.8793027883366946 1105 4 0.9505087525766029 1105 5 0.7085136960970146 1105 6 0.62703918881666 1105 7 0.96913882449266 1105 8 1.022449778075483 1105 9 0.9703340761533639 1105 10 1.208369008341542 1105 11 1.1531647022210834 1105 12 1.2410405321128692 1105 13 1.1564803958466285 1105 14 1.3034831217018237 1105 15 1.237831260554458 1105 16 1.2262773415220423 1105 17 1.1314520448739103 1105 18 1.2379494413125443 1105 19 1.205832007270729 1105 20 1.2263683717057106 1105 21 1.1439239841806597 1105 22 0.7286628648496389 1105 23 1.1486179876759692 1105 24 1.160459198143883 1105 25 1.1809564353858093 1106 1 0.6826957443072033 1106 2 0.6878568121076043 1106 3 0.8742194425400136 1106 4 0.6461532662115061 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0.9377241883482696 1107 18 1.1813308500382789 1107 19 0.9342032473834934 1107 20 1.0316540954869708 1107 21 0.9759659232159024 1107 22 0.9637424291492849 1107 23 1.0780828354428138 1107 24 0.9824931106496848 1107 25 0.9586506151186285 1108 1 0.3012365078848873 1108 2 0.5163942019335224 1108 3 0.4519908020509966 1108 4 0.556872863048163 1108 5 0.4976147458582645 1108 6 0.5865053751361486 1108 7 0.5080157003073609 1108 8 0.5549424285036864 1108 9 0.7041970834122466 1108 10 0.7564820767644562 1108 11 0.5448927824728145 1108 12 0.7049681613610872 1108 13 0.6148614503088865 1108 14 1.0074881933725095 1108 15 0.8964678761409175 1108 16 0.8044192658074678 1108 17 0.8385345290207056 1108 18 1.099547526101432 1108 19 1.0176623458736813 1108 20 0.8804418397374008 1108 21 0.8413450938798377 1108 22 0.6539675973353951 1108 23 0.8076419283799083 1108 24 0.848742994732369 1108 25 0.9530843956260782 1109 1 0.2894601941096262 1109 2 -0.009634983485458548 1109 3 0.401620487346122 1109 4 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-3.8144603726787984 2216 23 -3.75468255976514 2216 24 -3.7935959872949 2216 25 -3.711874260257055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59014.0 3 0.0 4 494.0 5 2918.0 6 3763.0 7 3334.0 8 2923.0 9 2753.0 10 2443.0 11 2402.0 12 2222.0 13 2227.0 14 2112.0 15 2168.0 16 2321.0 17 2389.0 18 2705.0 19 3361.0 20 4087.0 21 5384.0 22 6784.0 23 8054.0 24 9343.0 25 10611.0 26 11628.0 27 13220.0 28 14790.0 29 17745.0 30 21750.0 31 27260.0 32 34469.0 33 44161.0 34 57403.0 35 75980.0 36 103982.0 37 162415.0 38 454647.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22414192310661 18.79190304827316 12.678987770776914 25.30496725784332 2 14.774968696921094 20.42524841666871 41.167289910051736 23.63249297635846 3 18.653891838927606 24.75011561089473 29.934048951385932 26.661943598791726 4 12.19552197922972 17.021010378376182 38.50896652474883 32.27450111764527 5 12.997128827384696 38.00059520186136 36.021718104282066 12.980557866471873 6 31.56879103156752 37.2720257158627 18.328215349218947 12.830967903350821 7 27.46913162841648 31.765515329375333 22.771256237912986 17.9940968042952 8 24.703529413753667 36.887542783142194 20.968621328299808 17.44030647480433 9 26.191094044128278 15.161138446271547 20.41813920109953 38.229628308500644 10 14.662221673735942 29.186495291001087 35.088423689873835 21.062859345389136 11 33.71674421946385 22.649220457033216 25.011136328096935 18.622898995406 12 22.74357489719413 25.081166978711895 32.09115383477522 20.084104289318756 13 28.509120416546917 22.238094433080317 25.958700319510424 23.294084830862342 14 20.656270129624215 22.453544420004768 28.032271644470896 28.857913805900125 15 23.05645256733224 30.771467699122525 23.66620454122779 22.505875192317447 16 22.044356836021038 28.336272085014734 27.80856470015435 21.810806378809882 17 21.526003254369098 28.650789290167154 28.118678810676023 21.704528644787725 18 21.46732165199097 27.777293710499755 29.635745482876846 21.119639154632424 19 23.043487449122345 27.49578397021095 27.904411298537173 21.556317282129527 20 23.155340175127616 27.715268426733886 28.217202877712438 20.91218852042606 21 22.670178710152438 27.16603957642456 27.63282802624227 22.530953687180734 22 22.732620944633503 28.073084927079055 27.46626973058048 21.728024397706967 23 22.055173859532857 27.973312460271305 27.739166745560528 22.23234693463531 24 22.57483739270454 27.44312442680664 28.315012277431922 21.667025903056892 25 22.512449060498504 27.458768694723084 27.985302036058528 22.043480208719878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 252.0 1 252.0 2 338.0 3 424.0 4 424.0 5 424.0 6 845.0 7 1266.0 8 1266.0 9 1266.0 10 1839.0 11 2412.0 12 2412.0 13 2412.0 14 4058.0 15 5704.0 16 5704.0 17 5704.0 18 10286.0 19 14868.0 20 14868.0 21 14868.0 22 23276.5 23 31685.0 24 31685.0 25 31685.0 26 45014.0 27 58343.0 28 58343.0 29 58343.0 30 74316.0 31 90289.0 32 90289.0 33 90289.0 34 102497.5 35 114706.0 36 114706.0 37 114706.0 38 124030.5 39 133355.0 40 133355.0 41 133355.0 42 140310.0 43 147265.0 44 147265.0 45 147265.0 46 151670.0 47 156075.0 48 156075.0 49 156075.0 50 149051.5 51 142028.0 52 142028.0 53 142028.0 54 126507.0 55 110986.0 56 110986.0 57 110986.0 58 95243.0 59 79500.0 60 79500.0 61 79500.0 62 64301.5 63 49103.0 64 49103.0 65 49103.0 66 37635.5 67 26168.0 68 26168.0 69 26168.0 70 19026.0 71 11884.0 72 11884.0 73 11884.0 74 8257.0 75 4630.0 76 4630.0 77 4630.0 78 3133.5 79 1637.0 80 1637.0 81 1637.0 82 1060.0 83 483.0 84 483.0 85 483.0 86 309.5 87 136.0 88 136.0 89 136.0 90 88.5 91 41.0 92 41.0 93 41.0 94 25.0 95 9.0 96 9.0 97 9.0 98 11.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3550354866462373 2 0.0398052164271311 3 0.035072536767005114 4 0.035326073177369 5 0.039720704290343134 6 0.015127672485045577 7 0.014705111801105756 8 0.022057667701658634 9 0.01783206086226043 10 0.01757852445189654 11 0.01664889094722893 12 0.017494012315108574 13 0.017071451631168754 14 0.025691689583541094 15 0.027212908045724446 16 0.018592670093352106 17 0.020029376418747497 18 0.09710444516937078 19 0.023409861890266063 20 0.020198400692323426 21 0.024085958984569774 22 0.01690242735759282 23 0.02011388855553546 24 0.01664889094722893 25 0.020367424965899352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1183262.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.56421025656861 #Duplication Level Percentage of deduplicated Percentage of total 1 84.62492222148099 63.94615415689756 2 10.9750820415268 16.586468139380433 3 2.5363111477396774 5.749630465316395 4 0.8567535292256335 2.589596152818519 5 0.3543861813173712 1.3389455958544143 6 0.1709756507372413 0.7751784012637526 7 0.10666216077165906 0.5641889360078699 8 0.0706084733703667 0.4268378822122972 9 0.044852134622115146 0.30502945179372865 >10 0.22880991299604167 3.0235741460109256 >50 0.015503568706294005 0.8139190827412829 >100 0.01300315729286356 2.1244726112707424 >500 0.0011209580068494082 0.537618233471985 >1k 0.0010088622061644674 1.2183867449599861 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2265 0.19141998982473873 No Hit GTACATGGGAAGCAGTGGTATCAAC 1753 0.1481497757893011 No Hit GTCCTACAGTGGACATTTCTAAATT 1607 0.13581100381825834 No Hit CCCATGTACTCTGCGTTGATACCAC 1574 0.1330221033042555 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1562 0.13200795766279996 No Hit CTGTAGGACGTGGAATATGGCAAGA 1508 0.1274443022762499 No Hit GGTATCAACGCAGAGTACTTTTTTT 1436 0.12135942842751647 No Hit TATCAACGCAGAGTACTTTTTTTTT 1405 0.11873955218708958 No Hit CTTTAGGACGTGAAATATGGCGAGG 1274 0.10766846226786628 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.451213678796411E-5 0.0 4 0.0 0.0 0.0 8.451213678796411E-5 0.0 5 0.0 0.0 0.0 8.451213678796411E-5 0.0 6 0.0 0.0 0.0 8.451213678796411E-5 0.0 7 0.0 0.0 0.0 8.451213678796411E-5 0.0 8 0.0 0.0 0.0 1.6902427357592823E-4 0.0 9 0.0 0.0 0.0 2.5353641036389234E-4 0.0 10 0.0 0.0 0.0 2.5353641036389234E-4 0.0 11 0.0 0.0 0.0 2.5353641036389234E-4 0.0 12 0.0 0.0 0.0 2.5353641036389234E-4 0.0 13 0.0 0.0 0.0 3.3804854715185646E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCCG 130 2.0008883E-10 12.415975 19 TAGGACC 290 0.0 11.791346 4 GGCGAGG 275 0.0 11.73874 19 GTCCTAC 875 0.0 11.5429735 1 CTACACT 165 7.2759576E-12 11.513434 4 GGACAGT 175 1.8189894E-12 11.393482 6 AGGACCT 510 0.0 11.174804 5 GTCCTAA 490 0.0 11.084054 1 TAGAAGT 140 8.356437E-9 10.855524 4 GGGTATA 80 3.6666766E-4 10.719447 1 GGACTGT 170 1.4006218E-10 10.611576 6 CTTATAC 90 9.5241274E-5 10.553982 3 GACGTGA 450 0.0 10.549521 7 TCCTACA 1010 0.0 10.533083 2 TACACTA 110 6.050859E-6 10.362091 5 GTATTAT 130 3.6824713E-7 10.261352 1 GAATAAC 75 0.0025820257 10.163624 1 GATATAC 275 0.0 10.048128 1 CCTACAG 975 0.0 10.034401 3 CGTGAAA 550 0.0 9.840657 9 >>END_MODULE