FastQCFastQC Report
Thu 26 May 2016
SRR1512999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183262
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28460.2405215412985459No Hit
TATCAACGCAGAGTACTTTTTTTTT20980.17730646298114872No Hit
GGTATCAACGCAGAGTACTTTTTTT18740.15837574434064475No Hit
CCCATGTACTCTGCGTTGATACCAC17490.14781172724214925No Hit
GTACATGGGAAGCAGTGGTATCAAC17160.14502282672814643No Hit
GTCCTACAGTGGACATTTCTAAATT15850.13395173680892314No Hit
CTGTAGGACGTGGAATATGGCAAGA14100.1191621128710294No Hit
GTCCTAAAGTGTGTATTTCTCATTT13890.11738735799848217No Hit
CTTTAGGACGTGAAATATGGCGAGG13180.1113869962865367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA400.005273048814.25206310
AGGGTAT604.0610228E-412.6776176
AAGTCGT1052.7035276E-711.7665937
TAGGACC2350.011.7385094
TAGTACC907.350098E-611.6260714
GGCGAGG4850.011.350944519
CTACACT2500.011.0341994
TATACTG1900.011.01417355
TTAGGAC6750.010.9835483
GACGTGA4950.010.751767
CTAGACT1252.1980304E-710.6537094
TGGCGAG9450.010.64591818
CATCTAA1659.1858965E-1010.3695231
TAGGACT1752.2919266E-1010.3275754
CTAGGAC1303.8089274E-710.2361473
TTTAGGA8650.010.108932
GGACGTG8300.010.08099656
CTAGTAC856.558686E-410.0641123
GACTTAT856.566347E-410.0628347
GTCCTAC7500.010.012351
Produced by FastQC (version 0.11.5)