##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512999_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1183262
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.191556054364966	31.0	30.0	34.0	26.0	34.0
2	30.343596768931988	31.0	31.0	34.0	26.0	34.0
3	30.43549949208206	31.0	31.0	34.0	26.0	34.0
4	33.88808395773717	37.0	35.0	37.0	30.0	37.0
5	33.51382026972894	37.0	35.0	37.0	28.0	37.0
6	33.36042398048784	37.0	35.0	37.0	27.0	37.0
7	33.20449655274994	37.0	35.0	37.0	26.0	37.0
8	33.0831667035703	37.0	35.0	37.0	26.0	37.0
9	34.50648630649847	39.0	35.0	39.0	27.0	39.0
10	34.39823978121498	39.0	35.0	39.0	26.0	39.0
11	34.41331928178206	39.0	35.0	39.0	26.0	39.0
12	34.26071233589856	39.0	35.0	39.0	25.0	39.0
13	34.24315324923813	39.0	35.0	39.0	25.0	39.0
14	35.269919088080236	40.0	36.0	41.0	25.0	41.0
15	35.276325108048766	40.0	36.0	41.0	25.0	41.0
16	35.249015856167105	40.0	36.0	41.0	25.0	41.0
17	35.22768330259909	40.0	36.0	41.0	25.0	41.0
18	35.154076611942244	40.0	35.0	41.0	25.0	41.0
19	35.135473800392475	40.0	35.0	41.0	24.0	41.0
20	35.113907148205556	40.0	35.0	41.0	24.0	41.0
21	35.0610811468635	40.0	35.0	41.0	24.0	41.0
22	35.01758021469463	40.0	35.0	41.0	24.0	41.0
23	34.911657773172806	40.0	35.0	41.0	22.0	41.0
24	34.88904486073245	40.0	35.0	41.0	22.0	41.0
25	34.79023665088543	40.0	35.0	41.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.64516855099167
1101	2	0.6697656000887164
1101	3	0.674230020837701
1101	4	0.551112084006462
1101	5	0.7576819332474329
1101	6	0.7338455604485858
1101	7	0.8369188759392756
1101	8	0.7784785406622277
1101	9	0.6438304281954643
1101	10	1.0604938468047465
1101	11	0.9002003774995799
1101	12	0.9040287644984346
1101	13	0.9619207949085222
1101	14	1.1862719662811259
1101	15	1.1000973179046412
1101	16	1.3574046456757927
1101	17	1.1793561286706762
1101	18	0.5852607413623119
1101	19	0.8655246025846992
1101	20	0.8964104975806393
1101	21	1.1894618754765247
1101	22	1.1156638617203143
1101	23	1.1635215365390508
1101	24	1.0558736966072573
1101	25	1.1103859156407339
1102	1	0.6159998460797205
1102	2	0.6120011934039447
1102	3	0.535003718861681
1102	4	0.5177778634819319
1102	5	0.6417857003530116
1102	6	0.6550449328067032
1102	7	0.7187670359200382
1102	8	0.5370829950350569
1102	9	0.7154579620823469
1102	10	0.7724289851744501
1102	11	0.6079189102811355
1102	12	0.7580081076281928
1102	13	0.8117557314575237
1102	14	0.9009895197871671
1102	15	0.8912818802884033
1102	16	0.9435544286351742
1102	17	0.9101465273040361
1102	18	0.855798988021661
1102	19	0.8629508303425908
1102	20	0.903027720164367
1102	21	0.7260299078975478
1102	22	0.9064507177074645
1102	23	0.9524615052059033
1102	24	0.9001633822754158
1102	25	0.9486102728324752
1103	1	0.7833857353675491
1103	2	0.6917636291488307
1103	3	0.6650985993460559
1103	4	0.6322974078540824
1103	5	0.7898484521686058
1103	6	0.953702854801108
1103	7	0.9628376354171095
1103	8	0.8472922418443432
1103	9	1.073059736701559
1103	10	1.0188026183872125
1103	11	1.1284709152481724
1103	12	1.2067126326404605
1103	13	1.1635695446216587
1103	14	1.2815949589298512
1103	15	1.0905789082161306
1103	16	1.2168502325853936
1103	17	1.4263016968308477
1103	18	1.4473123487882873
1103	19	1.4578907354176494
1103	20	1.3929586528618998
1103	21	0.8740244963165864
1103	22	1.3271217122558738
1103	23	1.3293324793052932
1103	24	1.2475975490297557
1103	25	1.2711393126621289
1104	1	0.454264638757337
1104	2	0.46685704081698987
1104	3	0.5277274074030203
1104	4	0.4100937769421762
1104	5	0.48366987022804864
1104	6	0.4113120090720912
1104	7	0.45037361177746504
1104	8	0.4020272020945441
1104	9	0.4676851403255071
1104	10	0.5795075158119971
1104	11	0.632976569245848
1104	12	0.6392745554781456
1104	13	0.6194268948186306
1104	14	0.817266544238592
1104	15	0.7278104720079313
1104	16	0.7124048956291702
1104	17	0.7116334913063298
1104	18	0.7580506670737748
1104	19	0.7449743339671429
1104	20	0.7087308342364054
1104	21	0.48260749532048663
1104	22	0.6696117331203553
1104	23	0.5698756806612693
1104	24	0.6222902003552093
1104	25	0.8165149819855486
1105	1	0.5010478150951698
1105	2	0.5579412024621675
1105	3	0.5670036314681397
1105	4	0.4472093507841848
1105	5	0.47509255974212294
1105	6	0.616816163679772
1105	7	0.7060311988404919
1105	8	0.7358215165134965
1105	9	0.7657638689999615
1105	10	0.7514090671236104
1105	11	0.8182040080880881
1105	12	0.7441627268787485
1105	13	0.7727359917367522
1105	14	1.092107088309902
1105	15	0.9308439914092901
1105	16	1.0814652742326345
1105	17	0.7810308377424278
1105	18	1.1395300899065859
1105	19	1.0668889722496218
1105	20	1.1182732871565335
1105	21	0.9785479342915764
1105	22	1.0826027328667038
1105	23	1.0439120549289882
1105	24	0.9657602063469071
1105	25	1.1759424255656796
1106	1	0.5342716975862061
1106	2	0.5577769128955765
1106	3	0.5423012788415456
1106	4	0.49079707234849224
1106	5	0.6331204277847391
1106	6	0.6017134203839234
1106	7	0.6617438303287955
1106	8	0.7183818957617945
1106	9	0.6814396996253436
1106	10	0.7139986332631949
1106	11	0.7340769861933936
1106	12	0.7340645836907598
1106	13	0.6635684073145711
1106	14	0.9332764674539646
1106	15	0.8357203323520608
1106	16	0.8375887429405751
1106	17	0.8965949514625322
1106	18	0.9114689787199381
1106	19	0.9854783933611699
1106	20	1.1623204529915583
1106	21	0.9946188529988333
1106	22	1.049464394253441
1106	23	1.1354983896627502
1106	24	1.0336865774283126
1106	25	1.0272031497087681
1107	1	0.44942108837196315
1107	2	0.42617709194071196
1107	3	0.38098950991509284
1107	4	0.5151360205899636
1107	5	0.5567950329967957
1107	6	0.5615146605347903
1107	7	0.6610172822795306
1107	8	0.5999126000279418
1107	9	0.563099586273978
1107	10	0.7738503944222472
1107	11	0.7404793308947717
1107	12	0.7039848361970655
1107	13	0.5304233346762217
1107	14	0.6751618390713645
1107	15	0.7829497405157326
1107	16	0.6968367204546126
1107	17	0.7171704960032983
1107	18	0.9139078104504179
1107	19	0.9777570718500854
1107	20	1.0119645020446555
1107	21	0.8974506624737444
1107	22	0.9827182433947499
1107	23	0.7416501040857568
1107	24	0.9883213450975035
1107	25	0.9365097118446712
1108	1	0.4703318879812244
1108	2	0.4112978223880255
1108	3	0.35300495023812317
1108	4	0.4256270641138755
1108	5	0.5554250886671994
1108	6	0.4650112738343566
1108	7	0.3554141060220246
1108	8	0.4485028403235063
1108	9	0.5851582066514069
1108	10	0.591148567674793
1108	11	0.5927392603240378
1108	12	0.7198767922330092
1108	13	0.6870026646563687
1108	14	0.8780468192785662
1108	15	0.8212759027047767
1108	16	0.531797820108487
1108	17	0.6948265617409319
1108	18	0.7340662348831373
1108	19	0.5259689635030256
1108	20	0.7455286578670695
1108	21	0.8393541355616421
1108	22	0.8483857370066445
1108	23	0.7256779975528858
1108	24	0.7777747433281235
1108	25	0.7594684797482572
1109	1	0.1005465619623358
1109	2	0.05002458552299416
1109	3	0.10728687927018399
1109	4	0.20257793096479304
1109	5	0.1393842413270363
1109	6	-0.011067036383877848
1109	7	0.19729905101060297
1109	8	0.25692586468551326
1109	9	0.38209437781166855
1109	10	0.2905306806813144
1109	11	0.31175932495168013
1109	12	0.3954218270100327
1109	13	0.3732730486647853
1109	14	0.545017141219958
1109	15	0.443419079924233
1109	16	0.40974231604351274
1109	17	0.39536429042174603
1109	18	0.3126812217043309
1109	19	0.6212630025635022
1109	20	0.46950851809101124
1109	21	0.4317563521616492
1109	22	0.4623218719843365
1109	23	0.45141493912360175
1109	24	0.11113952766607582
1109	25	0.04665094289073579
1110	1	0.45164306956694844
1110	2	0.5346964353281436
1110	3	0.5153813152788906
1110	4	0.5418060471401134
1110	5	0.4583289720333994
1110	6	0.5261459065269207
1110	7	0.6279061198821765
1110	8	0.5444476109108649
1110	9	0.6675840792971925
1110	10	0.7062468748083788
1110	11	0.6246560466304203
1110	12	0.7837763603201324
1110	13	0.41833316366739837
1110	14	0.7579647539062009
1110	15	0.82915466241883
1110	16	0.6927643568282278
1110	17	0.5835213444962051
1110	18	0.5174151917284249
1110	19	0.6133961931807477
1110	20	0.7088483732175916
1110	21	0.7303543602212343
1110	22	0.6627148943846777
1110	23	0.761095293811799
1110	24	0.717815930003745
1110	25	0.6997194770361119
1111	1	-0.44705719045940384
1111	2	-0.20348764496996452
1111	3	-0.23276671056927256
1111	4	-0.21821050813199605
1111	5	0.05705699208329662
1111	6	-0.10584740868031162
1111	7	-0.026551826480535112
1111	8	0.19302026437733844
1111	9	-0.4755333937347288
1111	10	-0.3914549758225405
1111	11	-0.053489986764439834
1111	12	-0.12897741162903742
1111	13	-0.020088835703525376
1111	14	0.07008200418631816
1111	15	0.3237126344147896
1111	16	-0.005169368354252413
1111	17	0.3244030722442517
1111	18	0.06208924091963297
1111	19	0.1974449814221444
1111	20	0.11007138135195049
1111	21	-0.1012665453152124
1111	22	-0.3063156091799115
1111	23	-0.15712261341823108
1111	24	-0.14557732990731154
1111	25	-0.04603228790812608
1112	1	0.20289319201852507
1112	2	-0.013765503093576825
1112	3	0.08812118012584236
1112	4	-0.1105453440912072
1112	5	0.3508004397693938
1112	6	0.27624924030773457
1112	7	0.19294025700951778
1112	8	0.3470975328069912
1112	9	0.036881847761982556
1112	10	0.3469269213274231
1112	11	0.11055422999324804
1112	12	0.4040413915826093
1112	13	0.4625416017881179
1112	14	0.3959359343170732
1112	15	0.3792925477784763
1112	16	0.09942499697018548
1112	17	0.3085457580612996
1112	18	0.28686461873876823
1112	19	0.3647769306476363
1112	20	0.5942892365716759
1112	21	0.6981574798548209
1112	22	0.36508234735559597
1112	23	0.6807815721741619
1112	24	0.6453505520532872
1112	25	0.52332306158101
1113	1	-0.04490752148534227
1113	2	-0.5443523802520502
1113	3	-0.09625250399171748
1113	4	-0.11309406058767024
1113	5	-0.07068165625219791
1113	6	-0.2721225270017982
1113	7	-0.01164766060420419
1113	8	-0.011726764159220693
1113	9	0.06859920860679125
1113	10	-0.2858054816740605
1113	11	-0.10049864120169616
1113	12	-0.10751900069067233
1113	13	-0.01914957673542972
1113	14	-0.1305640505832173
1113	15	0.10360666497840754
1113	16	-0.34311691611134165
1113	17	-0.09728364032650916
1113	18	0.025699240485423047
1113	19	-0.20645294329545294
1113	20	-0.07257106721162643
1113	21	-0.23873429399601775
1113	22	-0.25731131509815697
1113	23	-0.015888116333137248
1113	24	-0.3773331957052619
1113	25	-0.49123875348348633
1114	1	-0.4248313465141642
1114	2	-0.30486257218106516
1114	3	-0.37128311620121934
1114	4	-0.13386064350194715
1114	5	-0.13720005075021646
1114	6	-0.668217345646859
1114	7	-0.4751315439552073
1114	8	-0.7293966286792326
1114	9	-0.5534946549604598
1114	10	-0.7771282644424176
1114	11	-0.5059933449136693
1114	12	-0.6008704566061951
1114	13	-0.6357193673698873
1114	14	-1.7547432444559234
1114	15	-1.039367095819692
1114	16	-0.8909673550886907
1114	17	-0.8679678391075711
1114	18	-1.146805040598835
1114	19	-1.1449255819349418
1114	20	-0.47515948995727797
1114	21	-0.4108078650504581
1114	22	-0.4589292816611845
1114	23	-0.33596116937926723
1114	24	-1.265401198170423
1114	25	-1.0195812078484536
1115	1	-1.7653163184480185
1115	2	-1.3563384285579545
1115	3	-1.2771356490723882
1115	4	-2.416545777498172
1115	5	-1.6160311804404124
1115	6	-1.5734421425124552
1115	7	-1.1975991390014187
1115	8	-1.1651485854289163
1115	9	-1.7352225742637515
1115	10	-1.6240238437010817
1115	11	-1.2887007369187131
1115	12	-1.9830654331540742
1115	13	-1.3316686503912933
1115	14	-2.206179577827953
1115	15	-2.3621040127726545
1115	16	-1.6777569862253614
1115	17	-1.5583342721052489
1115	18	-1.6795306300300084
1115	19	-1.4464921246585902
1115	20	-1.6149683598339521
1115	21	-1.6500194984093142
1115	22	-2.010080291422824
1115	23	-2.405699421211189
1115	24	-1.8323436636238881
1115	25	-1.4795661205278776
1116	1	-1.6479158283605173
1116	2	-1.6859883249085215
1116	3	-1.5080674477120475
1116	4	-1.6632264627465787
1116	5	-1.5644888881274888
1116	6	-2.2663007776641315
1116	7	-1.7766718531928625
1116	8	-1.7074048089474871
1116	9	-2.068612193825132
1116	10	-2.078152057768868
1116	11	-2.1294196511110144
1116	12	-2.7681838179909626
1116	13	-2.3823620139470236
1116	14	-2.9895438189223995
1116	15	-2.6454376275371274
1116	16	-3.0595405113788487
1116	17	-2.782830899401354
1116	18	-3.3964459990507976
1116	19	-3.120603141628223
1116	20	-2.915277238787766
1116	21	-2.859328445899891
1116	22	-4.0853161058862
1116	23	-3.9402301943819964
1116	24	-3.243837708706508
1116	25	-3.611672215432421
1201	1	0.8413145102795205
1201	2	0.8403873146165779
1201	3	0.7753382379123543
1201	4	0.7181927643039145
1201	5	0.8456500882931053
1201	6	0.8184763394395844
1201	7	0.8963861499882881
1201	8	0.8567545148423861
1201	9	0.9631810599924435
1201	10	0.987694725000722
1201	11	1.076685864495289
1201	12	1.0226890285660701
1201	13	1.0457019326910455
1201	14	1.0385747344493268
1201	15	1.1055455546327622
1201	16	1.1161424642379743
1201	17	0.953837424387217
1201	18	1.0795759689054236
1201	19	0.9357918483833743
1201	20	1.1081455921766619
1201	21	0.9514853183895227
1201	22	1.115332650815688
1201	23	0.9801004079622473
1201	24	1.1983704910458357
1201	25	1.1431249567608148
1202	1	0.9505116050758318
1202	2	0.8904615034476784
1202	3	0.9171335005360568
1202	4	0.7999462384472906
1202	5	1.0534394991017066
1202	6	1.1055344637412787
1202	7	1.035530664119861
1202	8	0.9892122176896478
1202	9	1.2935956250388543
1202	10	1.2464254475059349
1202	11	1.452286008708377
1202	12	1.1939173861907406
1202	13	1.2082920807474409
1202	14	1.2151164878282756
1202	15	1.4959873321662158
1202	16	1.4191642758733067
1202	17	1.6064391499468442
1202	18	1.6111785071510454
1202	19	1.6499768480799588
1202	20	1.534839054873423
1202	21	1.465420265434048
1202	22	1.5822844120121289
1202	23	1.4774133682215407
1202	24	1.2752133428002494
1202	25	1.452534920552857
1203	1	0.4952076141561399
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2211	9	-0.1625417291724034
2211	10	-0.1519252299395859
2211	11	0.10514151124766613
2211	12	0.0640333788050853
2211	13	0.01917468800904487
2211	14	-1.0101441770825588
2211	15	-0.24124483911303685
2211	16	0.06673128355601676
2211	17	0.2374525447257554
2211	18	-0.018598384562054093
2211	19	0.0407546631445328
2211	20	0.021295176699460683
2211	21	0.035421723357430324
2211	22	0.23732823741904951
2211	23	0.17572502393078082
2211	24	0.07889461371836148
2211	25	-0.03786104511146959
2212	1	-0.017306197237083154
2212	2	0.00887804332437625
2212	3	0.0189702316689484
2212	4	0.2683414249765761
2212	5	0.018865383249000445
2212	6	0.275637260157346
2212	7	0.195498272250326
2212	8	-0.024932193869020125
2212	9	-0.12446830571050782
2212	10	-0.27260735026313654
2212	11	-0.031142906956340255
2212	12	0.0749530678774093
2212	13	-0.013681185948577479
2212	14	0.15158706125647115
2212	15	0.2778776912371299
2212	16	0.17040907734152455
2212	17	-0.04215397481877403
2212	18	0.20171484087997982
2212	19	0.121971067658734
2212	20	-0.15557602387937663
2212	21	-0.04965165086655077
2212	22	0.03249936141484966
2212	23	-0.4594877047090762
2212	24	-0.18224629822895366
2212	25	-0.18529743292928202
2213	1	-0.24827264051540254
2213	2	-0.28154077774968655
2213	3	-0.32734984804946876
2213	4	-0.25431973324994317
2213	5	-0.271959157821243
2213	6	-0.05265949992755736
2213	7	-0.6503458063903551
2213	8	-0.13949948052032113
2213	9	-0.35784625968994277
2213	10	-0.26026297011233623
2213	11	-0.2046944110142519
2213	12	-0.44007952800352257
2213	13	-0.2760494445601509
2213	14	-0.26449376341290076
2213	15	-0.35632282225670053
2213	16	-0.695664833266072
2213	17	-0.6195408586297475
2213	18	-0.6888157456462949
2213	19	-0.9769174078120315
2213	20	-0.3685867200042239
2213	21	-1.073883085185102
2213	22	-0.6472535571032836
2213	23	-0.4939328476875957
2213	24	-0.6265943734824617
2213	25	-0.7210739248341298
2214	1	-0.9891359777202027
2214	2	-0.9186073116803293
2214	3	-0.6838317904199123
2214	4	-1.2660917591334666
2214	5	-1.4261486384915543
2214	6	-2.616773763519177
2214	7	-1.1010758545071653
2214	8	-1.7659388367506175
2214	9	-1.3331739517423742
2214	10	-1.4423491379127924
2214	11	-1.3050207203997957
2214	12	-1.2521972206090197
2214	13	-1.5776339808136726
2214	14	-1.6227455193998424
2214	15	-1.9584327719276189
2214	16	-1.3596076461635889
2214	17	-1.5139327617324554
2214	18	-1.558822935514108
2214	19	-2.9709138681847946
2214	20	-1.6756161984622722
2214	21	-2.1997251293198303
2214	22	-1.9076705694366751
2214	23	-1.836831470551381
2214	24	-1.155375967420845
2214	25	-1.1555410450155321
2215	1	-1.4406299086258976
2215	2	-1.3033211972345526
2215	3	-1.290907954218767
2215	4	-1.1014058847837305
2215	5	-1.7189724346766972
2215	6	-1.5242634260508545
2215	7	-1.6177025051674256
2215	8	-2.348458511287099
2215	9	-1.8945318282262988
2215	10	-1.4400949028854484
2215	11	-1.9722742884937006
2215	12	-2.9620945128592524
2215	13	-2.2939538420915184
2215	14	-2.3485091581886977
2215	15	-3.4291610632441483
2215	16	-2.852131322300714
2215	17	-2.3670073248910484
2215	18	-3.1706676734092696
2215	19	-3.6970376510312697
2215	20	-3.314244983309546
2215	21	-2.127783019142427
2215	22	-3.2359352086256834
2215	23	-3.1280746344836174
2215	24	-2.142424120015221
2215	25	-2.3285435793626093
2216	1	-2.2475472374909806
2216	2	-2.117259098679117
2216	3	-2.472875847116107
2216	4	-1.941824952713013
2216	5	-2.031118803798364
2216	6	-2.507185900176456
2216	7	-3.9190167468761956
2216	8	-2.919242232630623
2216	9	-2.9054485816346123
2216	10	-3.000242829125426
2216	11	-3.609777144621045
2216	12	-3.002731915856703
2216	13	-3.286545682453042
2216	14	-3.6662442280474536
2216	15	-3.625236109576882
2216	16	-3.4380173987856857
2216	17	-3.7468646454627823
2216	18	-3.3316161615658615
2216	19	-3.4336020048694813
2216	20	-4.001013708926681
2216	21	-3.8062110066516297
2216	22	-4.114236257141766
2216	23	-3.073380853648022
2216	24	-3.3407299075870434
2216	25	-3.1516210883118063
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37403.0
3	0.0
4	604.0
5	5228.0
6	6082.0
7	4736.0
8	3802.0
9	3178.0
10	2827.0
11	2478.0
12	2262.0
13	2023.0
14	2102.0
15	2069.0
16	2142.0
17	2178.0
18	2355.0
19	2931.0
20	3703.0
21	4687.0
22	5880.0
23	7098.0
24	8448.0
25	9690.0
26	11000.0
27	12007.0
28	13396.0
29	16324.0
30	20362.0
31	26066.0
32	33198.0
33	42404.0
34	55643.0
35	72779.0
36	99901.0
37	155242.0
38	501034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.255170150647345	19.633970587737785	13.57568396872706	27.53517529288781
2	13.5655730777682	20.890928451904063	39.86063925088315	25.682859219444587
3	16.674582636812474	24.959391918273383	30.529417829694523	27.83660761521962
4	11.30698975089016	17.709048364572098	37.57874501661972	33.40521686791802
5	12.585355044848928	38.23804521935633	36.25489887636648	12.921700859428253
6	31.520866722767966	37.85247613932856	17.882415626043972	12.744241511859505
7	27.07423100854886	31.9979405637918	22.71821907802186	18.209609349637486
8	24.71306262727402	37.15597959556461	20.66261983208286	17.468337945078513
9	25.831751520865126	15.015198082378255	20.241562844810456	38.91148755194615
10	14.271512392813479	29.482162724684525	35.03822122197921	21.208103660522788
11	33.74416523200888	22.714837361784774	24.774321358272292	18.766676047934048
12	22.491708336291623	25.25500458112307	32.096683672606915	20.156603409978395
13	28.353351132567106	22.084319010274704	26.135799871027395	23.426529986130795
14	20.56558181361781	22.207947963565704	27.990797610827173	29.235672611989305
15	22.82797941824771	31.00760321302519	23.581454786406848	22.58296258232025
16	21.946797333358127	28.370303813581888	27.73720036414014	21.94569848891984
17	21.380398230163305	28.778866783776607	28.05372066404381	21.787014322016272
18	21.325960407285706	27.844543155788138	29.66385745000203	21.165638986924126
19	22.943681600785652	27.403464616440402	27.978337061862597	21.674516720911345
20	22.93201531787291	27.668605206420256	28.250758751953402	21.148620723753435
21	22.489055659787365	27.074522928181466	27.793637915589137	22.642783496442036
22	22.564920385005117	27.966367407925752	27.590455835038373	21.878256372030762
23	21.84909174806594	27.84818577795957	27.919852069955763	22.38287040401872
24	22.476763387990147	27.37550939690449	28.375710535790045	21.772016679315318
25	22.306098870961335	27.356305130326675	28.113983970845634	22.223612027866356
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	134.0
1	134.0
2	265.5
3	397.0
4	397.0
5	397.0
6	868.5
7	1340.0
8	1340.0
9	1340.0
10	1905.5
11	2471.0
12	2471.0
13	2471.0
14	4145.0
15	5819.0
16	5819.0
17	5819.0
18	10485.0
19	15151.0
20	15151.0
21	15151.0
22	23629.5
23	32108.0
24	32108.0
25	32108.0
26	45676.5
27	59245.0
28	59245.0
29	59245.0
30	75092.5
31	90940.0
32	90940.0
33	90940.0
34	102911.5
35	114883.0
36	114883.0
37	114883.0
38	124260.5
39	133638.0
40	133638.0
41	133638.0
42	140358.0
43	147078.0
44	147078.0
45	147078.0
46	151359.5
47	155641.0
48	155641.0
49	155641.0
50	149025.5
51	142410.0
52	142410.0
53	142410.0
54	126661.5
55	110913.0
56	110913.0
57	110913.0
58	94743.0
59	78573.0
60	78573.0
61	78573.0
62	63460.5
63	48348.0
64	48348.0
65	48348.0
66	37159.0
67	25970.0
68	25970.0
69	25970.0
70	18806.5
71	11643.0
72	11643.0
73	11643.0
74	8045.5
75	4448.0
76	4448.0
77	4448.0
78	2968.5
79	1489.0
80	1489.0
81	1489.0
82	970.0
83	451.0
84	451.0
85	451.0
86	291.5
87	132.0
88	132.0
89	132.0
90	83.0
91	34.0
92	34.0
93	34.0
94	19.0
95	4.0
96	4.0
97	4.0
98	3.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07589189883559178
2	1.6902427357592823E-4
3	0.0
4	0.02788900514002816
5	0.07166629199619357
6	0.07411714396304453
7	0.03498802463021715
8	0.06541239387388423
9	0.04622813882301637
10	0.11214760551762838
11	0.004479143249762098
12	0.012338771971042762
13	0.005493288891217668
14	0.007690604447704735
15	0.006591946669461201
16	0.01698693949438079
17	0.027719980866452235
18	0.0011831699150314976
19	0.003634021881882457
20	0.0058313374383695245
21	1.6902427357592823E-4
22	4.225606839398206E-4
23	0.0
24	0.0
25	0.003634021881882457
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1183262.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.86369917482901
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.21457183572544	63.046143720467754
2	11.194508253022127	16.76124596528778
3	2.6597128306133246	5.973478237274065
4	0.8657319105933562	2.5924757328284387
5	0.3868776656870375	1.4481546590727221
6	0.18241523228650025	0.8193767444881865
7	0.10566665064801997	0.5537417442847582
8	0.0749799167115975	0.44906191430806147
9	0.04917531101373634	0.3313301121504911
>10	0.23497183266221666	3.045831524950407
>50	0.015257840468255608	0.8166506702260249
>100	0.013761885211772091	2.1365286876423535
>500	0.0012408185201205194	0.5896969840462354
>1k	0.0011280168364731996	1.436283302972701
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2846	0.2405215412985459	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2098	0.17730646298114872	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1874	0.15837574434064475	No Hit
CCCATGTACTCTGCGTTGATACCAC	1749	0.14781172724214925	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1716	0.14502282672814643	No Hit
GTCCTACAGTGGACATTTCTAAATT	1585	0.13395173680892314	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1410	0.1191621128710294	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1389	0.11738735799848217	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1318	0.1113869962865367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	8.451213678796411E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTGCGA	40	0.0052730488	14.252063	10
AGGGTAT	60	4.0610228E-4	12.677617	6
AAGTCGT	105	2.7035276E-7	11.766593	7
TAGGACC	235	0.0	11.738509	4
TAGTACC	90	7.350098E-6	11.626071	4
GGCGAGG	485	0.0	11.3509445	19
CTACACT	250	0.0	11.034199	4
TATACTG	190	0.0	11.0141735	5
TTAGGAC	675	0.0	10.983548	3
GACGTGA	495	0.0	10.75176	7
CTAGACT	125	2.1980304E-7	10.653709	4
TGGCGAG	945	0.0	10.645918	18
CATCTAA	165	9.1858965E-10	10.369523	1
TAGGACT	175	2.2919266E-10	10.327575	4
CTAGGAC	130	3.8089274E-7	10.236147	3
TTTAGGA	865	0.0	10.10893	2
GGACGTG	830	0.0	10.0809965	6
CTAGTAC	85	6.558686E-4	10.064112	3
GACTTAT	85	6.566347E-4	10.062834	7
GTCCTAC	750	0.0	10.01235	1
>>END_MODULE