FastQCFastQC Report
Thu 26 May 2016
SRR1512998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391393
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC58070.4173515318820779No Hit
CCCATGTACTCTGCGTTGATACCAC51160.36768907131198736No Hit
GAGTACATGGGAAGCAGTGGTATCA23510.1689673586111185No Hit
GTATCAACGCAGAGTACTTTTTTTT20430.14683126909507235No Hit
GTCCTAAAGTGTGTATTTCTCATTT19130.13748811442920872No Hit
GTCCTACAGTGGACATTTCTAAATT18260.13123538784513075No Hit
CATGTACTCTGCGTTGATACCACTG16890.1213891402357206No Hit
GCGTTGATACCACTGCTTCCCATGT16410.11793936005140174No Hit
CTTTAGGACGTGAAATATGGCGAGG15900.11427396860556292No Hit
CTGTAGGACGTGGAATATGGCAAGA15810.11362713482100312No Hit
GTATCAACGCAGAGTACATGGGAAG15310.11003361379567096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC658.0312835E-411.6907813
AGTCGTC752.0829766E-411.3944148
ATAGGAC1658.0035534E-1110.9379653
TGGCGAG9600.010.88751818
CAAGTCG700.001497341210.8518236
GGACCGT700.001497341210.8518236
CACCGCC700.001497341210.8518237
GTATAAT1951.8189894E-1210.74630451
GTATAGA2050.010.6867361
AGGACCG803.7759432E-410.685725
TTAGGAC6450.010.6032673
GGCGAGA5400.010.55038419
GTCCTAC10050.010.5202961
GTATAAA2001.8189894E-1210.4776471
AGGACGT9350.010.4634975
GGACGTG9700.010.2784666
GTGCTAG1409.313226E-810.20551
ATGGCGA10550.010.177320517
TATGTCG750.00264303410.1352916
GTCCTAA6350.010.050141
Produced by FastQC (version 0.11.5)