##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512998_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1391393
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.33515476935704	31.0	29.0	33.0	16.0	34.0
2	28.50112944365826	31.0	28.0	34.0	16.0	34.0
3	28.647854344530984	31.0	28.0	34.0	16.0	34.0
4	32.04233527119944	35.0	33.0	37.0	19.0	37.0
5	32.14185496117919	35.0	33.0	37.0	22.0	37.0
6	32.181654643943155	35.0	33.0	37.0	21.0	37.0
7	31.994661465164768	35.0	33.0	37.0	18.0	37.0
8	32.00022207959937	35.0	33.0	37.0	18.0	37.0
9	33.22275302520568	38.0	33.0	39.0	17.0	39.0
10	33.09749869375511	38.0	33.0	39.0	17.0	39.0
11	33.135791253801045	38.0	33.0	39.0	17.0	39.0
12	33.02326229900539	38.0	33.0	39.0	17.0	39.0
13	33.01204979470214	38.0	33.0	39.0	16.0	39.0
14	33.94459940505666	39.0	33.0	41.0	15.0	41.0
15	33.86009632073756	39.0	33.0	41.0	11.0	41.0
16	33.74552337118269	39.0	33.0	41.0	10.0	41.0
17	33.685430356484474	39.0	33.0	41.0	10.0	41.0
18	33.382066030230135	38.0	32.0	41.0	10.0	41.0
19	33.59473851025555	39.0	33.0	41.0	10.0	41.0
20	33.66992000103493	39.0	33.0	41.0	8.0	41.0
21	33.597910870616715	39.0	33.0	41.0	2.0	41.0
22	33.50966333738922	39.0	33.0	41.0	2.0	41.0
23	33.23418545299567	39.0	33.0	41.0	2.0	41.0
24	33.22749934777593	39.0	33.0	41.0	2.0	41.0
25	33.128301637280046	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6019198607071665
1101	2	0.9943896696472159
1101	3	0.9921656533642214
1101	4	1.034882355773508
1101	5	0.9052570843886798
1101	6	1.0049378913654863
1101	7	1.0837420163623257
1101	8	0.7864341365847132
1101	9	0.5700941892886888
1101	10	0.6050797588300938
1101	11	0.8579364192240675
1101	12	1.0250503267820719
1101	13	0.9221970450933838
1101	14	0.7835439482663134
1101	15	0.4578706881251762
1101	16	1.0568943703498803
1101	17	1.1358803462311258
1101	18	1.2936341533377345
1101	19	0.7960114931727773
1101	20	0.9662569968136978
1101	21	0.883643398934403
1101	22	0.9674858286012622
1101	23	1.2502594081989358
1101	24	0.9006237178618477
1101	25	1.148839002421802
1102	1	0.7067333316139219
1102	2	0.7514111780799375
1102	3	0.798501783815297
1102	4	1.0158988068978196
1102	5	0.8578393719535526
1102	6	0.818582025178884
1102	7	0.9837238139061917
1102	8	0.9777383896964587
1102	9	0.9443551203453637
1102	10	1.2078135624668604
1102	11	1.18809001318278
1102	12	1.1017193233910518
1102	13	1.0246113107918546
1102	14	1.2770127436219028
1102	15	1.5180493905812114
1102	16	1.299242583545258
1102	17	1.0038859372548217
1102	18	1.305576975422234
1102	19	1.4612773317708232
1102	20	1.3811827175963955
1102	21	1.138913261343525
1102	22	1.3340323953645807
1102	23	1.5989740613436965
1102	24	1.6183128396006694
1102	25	1.4973841988690353
1103	1	0.38104836993923996
1103	2	0.5721384669350691
1103	3	0.4822514534775024
1103	4	0.5366378887866396
1103	5	0.13498424783954022
1103	6	0.5766919840790337
1103	7	0.41932128026385485
1103	8	0.45871287443592834
1103	9	0.6639717588647684
1103	10	0.5617317008271456
1103	11	0.6257998019525814
1103	12	0.7848141424476935
1103	13	0.7972573589299117
1103	14	1.0023762889225836
1103	15	0.9217136925777112
1103	16	1.0005350550728451
1103	17	0.9448245033159424
1103	18	1.2914053052323027
1103	19	1.292789830240487
1103	20	1.1237925016448997
1103	21	0.9999939814911585
1103	22	1.0515367114718401
1103	23	1.4410019807888972
1103	24	1.1580178584255805
1103	25	1.1399463406965111
1104	1	0.628762880950287
1104	2	0.570852999367208
1104	3	0.6183752140349696
1104	4	0.7795073935188874
1104	5	0.5788120836990132
1104	6	0.5035921056134143
1104	7	0.8962934049572375
1104	8	0.8421736497905208
1104	9	0.8890694370205452
1104	10	0.9749061570244493
1104	11	1.098598451372979
1104	12	1.0029540076241545
1104	13	1.078422722884362
1104	14	1.2221764907658468
1104	15	1.2779355293999188
1104	16	1.1528198165530341
1104	17	1.0572425929021776
1104	18	1.4146527669340188
1104	19	1.1172848078029034
1104	20	1.145206115839727
1104	21	1.2406334697776629
1104	22	1.2407538595000744
1104	23	1.6489276229265286
1104	24	1.4386410262654934
1104	25	1.1467188410269245
1105	1	0.8756905938558042
1105	2	1.0055926478286992
1105	3	0.9427672505540556
1105	4	1.1329467643016038
1105	5	0.9081848196303213
1105	6	0.5884524708445724
1105	7	0.8300902745770848
1105	8	0.9006458728517508
1105	9	0.806402512609985
1105	10	1.1376073935910611
1105	11	0.9597175834204137
1105	12	0.9222052616086103
1105	13	0.9874143603031342
1105	14	1.1424059633775414
1105	15	1.3236645318303601
1105	16	1.2397284403013131
1105	17	1.1549055806774646
1105	18	1.2732534592180187
1105	19	1.3257005487058535
1105	20	1.3784995794142745
1105	21	1.2481722773496884
1105	22	1.0247336668767844
1105	23	1.3439920286959648
1105	24	1.395838222495179
1105	25	1.2917684466586294
1106	1	0.6989409116365195
1106	2	0.8121696898989903
1106	3	0.7868975749338745
1106	4	0.8557187463289786
1106	5	0.8006038985979629
1106	6	0.7823135139603821
1106	7	0.5060410078827893
1106	8	0.7861943290685751
1106	9	0.8923139196207401
1106	10	1.035852767011967
1106	11	0.8150129182305932
1106	12	0.9697130558079579
1106	13	0.8958813901193636
1106	14	1.0800746306464717
1106	15	1.180745401679907
1106	16	1.0999538236010054
1106	17	1.1143463263763707
1106	18	1.4665682872288315
1106	19	1.128714471426747
1106	20	0.9504521890920969
1106	21	0.8534082301501869
1106	22	0.8246533645105458
1106	23	1.1960212038972458
1106	24	1.121849729188206
1106	25	1.1394875314899267
1107	1	0.4847846173933483
1107	2	0.6100961733555152
1107	3	0.74909798354755
1107	4	0.8610684236894777
1107	5	0.8421677961899618
1107	6	0.6130458548496804
1107	7	0.9217111727103209
1107	8	0.8332989360795686
1107	9	0.9560572239958205
1107	10	0.788600558496185
1107	11	0.8649356379657078
1107	12	0.8455642449532874
1107	13	0.787606563602786
1107	14	1.117462333523008
1107	15	1.2509549463043115
1107	16	0.9544103968484521
1107	17	1.2975901248032429
1107	18	1.3893864623307692
1107	19	1.2919607220814768
1107	20	1.1197368071712575
1107	21	1.1386118810457262
1107	22	1.190026737643528
1107	23	1.2635943111837733
1107	24	1.2378490734414243
1107	25	1.3650870358981848
1108	1	0.576579877590973
1108	2	0.6290271654076669
1108	3	0.46224675715258456
1108	4	0.4270114624054564
1108	5	0.5151941229514279
1108	6	0.7989315347070445
1108	7	0.925779315540435
1108	8	0.9513218516356012
1108	9	1.0603582565971337
1108	10	0.9906905663959193
1108	11	0.9053425595976918
1108	12	0.9368612908819713
1108	13	1.0687763454025756
1108	14	1.082100622104278
1108	15	1.0546498980844916
1108	16	0.8642870969639063
1108	17	1.0668720617468352
1108	18	1.2575858806910034
1108	19	1.2224773716202293
1108	20	1.1161887317946366
1108	21	0.8992986154534321
1108	22	0.894585101077702
1108	23	1.0564088690852245
1108	24	1.0413057488321158
1108	25	1.211155887614936
1109	1	0.03378323974534325
1109	2	0.048771784978985266
1109	3	0.20711546675316939
1109	4	0.3254837010869771
1109	5	0.24928163736878872
1109	6	0.24723768771863064
1109	7	0.266427419253926
1109	8	0.42430384132354604
1109	9	0.5718348487452474
1109	10	0.6923032772199065
1109	11	0.14371294491722608
1109	12	0.6343264555224835
1109	13	0.5129022943867199
1109	14	0.43695351905059
1109	15	0.6903193819302018
1109	16	0.6931369189884364
1109	17	0.302966762454318
1109	18	-0.977618062655651
1109	19	0.4720024639910818
1109	20	0.765236784976473
1109	21	0.7565824684375997
1109	22	0.9103474974672707
1109	23	0.416286185103246
1109	24	0.7926809960637229
1109	25	0.8631030577834267
1110	1	0.29680786005136994
1110	2	0.17347080082989308
1110	3	0.3015506744381504
1110	4	-0.01661586960107897
1110	5	0.2008040447998809
1110	6	0.19428855621085717
1110	7	0.31481382842815364
1110	8	0.11400003877862375
1110	9	0.4857664083049755
1110	10	0.5215183579270786
1110	11	0.3708701229845843
1110	12	0.026262290833429347
1110	13	0.2012496881205621
1110	14	0.23163219824135695
1110	15	0.37016856222700767
1110	16	0.3922064536294414
1110	17	-0.047636942670429505
1110	18	0.5557712438610807
1110	19	0.342160073107209
1110	20	0.3996458455269831
1110	21	0.42761843811576483
1110	22	0.4540886850461163
1110	23	0.3185644278768649
1110	24	0.6043730756609094
1110	25	0.6013230316047142
1111	1	0.32154852190598504
1111	2	0.6173390948046844
1111	3	0.566396761967944
1111	4	0.4476937922591695
1111	5	0.5067483962713908
1111	6	0.584322702634779
1111	7	0.4440546621418342
1111	8	0.18931938672843174
1111	9	0.5895777352259941
1111	10	0.5978477300498497
1111	11	0.512436914178636
1111	12	0.5313631242090224
1111	13	0.5822851812433854
1111	14	0.6238512086712333
1111	15	0.8698558350772245
1111	16	0.6852965922999843
1111	17	0.6701141265183423
1111	18	0.8831726221144294
1111	19	0.5447297887227194
1111	20	0.6061218331531322
1111	21	0.8062225480830278
1111	22	0.5784977434274907
1111	23	0.9867242962497258
1111	24	0.9709442337656142
1111	25	0.3830084900739834
1112	1	-0.16982915235821494
1112	2	0.11962650243559025
1112	3	0.1926525268745145
1112	4	0.2674715235598164
1112	5	0.2925029180939873
1112	6	0.2626314872360709
1112	7	-0.1340109511522556
1112	8	0.10353882740416864
1112	9	0.2812927070718132
1112	10	0.37033522710777333
1112	11	0.36171588110092756
1112	12	0.37752787685376177
1112	13	0.1587262238486815
1112	14	0.26766504315622086
1112	15	0.3585765143998785
1112	16	0.40914639927758856
1112	17	0.5320496184567034
1112	18	0.4263756139867354
1112	19	0.2901382840475648
1112	20	0.3178784206309686
1112	21	0.3838000842738438
1112	22	0.5024551290525707
1112	23	0.4941714049273571
1112	24	0.23706262715293747
1112	25	0.49940318148924945
1113	1	-0.3055275143813816
1113	2	-0.06019525152529681
1113	3	-0.2444061652265539
1113	4	0.06835133409272132
1113	5	-0.17176892945848365
1113	6	-0.12963213815927332
1113	7	-0.341697675049641
1113	8	-0.07440176055950687
1113	9	-0.03820045850833509
1113	10	-0.3446820265130057
1113	11	-0.193335730809288
1113	12	-0.02800261612810573
1113	13	-0.2173494835497678
1113	14	-0.06379970235908416
1113	15	-0.45922564739191785
1113	16	-0.2900938455996993
1113	17	-0.3225385537518122
1113	18	-0.7170700457966888
1113	19	-0.3136770749022233
1113	20	-0.5357884330533622
1113	21	-0.4071609051654548
1113	22	-0.20420432171130898
1113	23	-0.7395803514664152
1113	24	-0.06372691839414557
1113	25	-0.11379774995801739
1114	1	-1.6839047494491162
1114	2	-1.5309799111965745
1114	3	-1.3007712460655867
1114	4	-1.4670218048554986
1114	5	-1.5874136297814267
1114	6	-2.0385484720807163
1114	7	-2.1477337250284876
1114	8	-1.4177387050813337
1114	9	-1.857223374078746
1114	10	-1.7265503522628443
1114	11	-2.602448926092041
1114	12	-1.9666429520718154
1114	13	-2.02425572677879
1114	14	-2.367650673768786
1114	15	-2.2855662753260084
1114	16	-3.1109929283578204
1114	17	-5.052709311379157
1114	18	-4.107131229647454
1114	19	-3.129858088304889
1114	20	-2.07221562633449
1114	21	-3.146714355914
1114	22	-3.039869657397073
1114	23	-3.163449069288145
1114	24	-2.615105257453788
1114	25	-2.3998721817117143
1115	1	-1.3569951828078963
1115	2	-0.9535876977145463
1115	3	-0.8290192242040355
1115	4	-0.833927443940766
1115	5	-0.6437726747768373
1115	6	-0.6884248370447068
1115	7	-2.1047214555649596
1115	8	-1.3754524802923704
1115	9	-1.242729586255276
1115	10	-0.8883379987164304
1115	11	-1.864534458303531
1115	12	-1.5443933475932958
1115	13	-1.2011243583297855
1115	14	-1.1745241732764597
1115	15	-2.472025611292466
1115	16	-1.2827446736309227
1115	17	-1.3241158742698431
1115	18	-1.4860919762149933
1115	19	-1.440991049903353
1115	20	-1.2334819035646234
1115	21	-1.960375335922187
1115	22	-2.1524273260883646
1115	23	-1.4849485658971737
1115	24	-1.2869621063369259
1115	25	-1.7093110390695188
1116	1	-1.9220338326346038
1116	2	-2.8080876740375835
1116	3	-2.3186154530272454
1116	4	-2.1669450844071783
1116	5	-2.6703261091769868
1116	6	-2.621719779870471
1116	7	-2.2216557846837937
1116	8	-3.0236869115603966
1116	9	-2.7017216465796707
1116	10	-3.656028637746438
1116	11	-2.252680247524424
1116	12	-2.104773272683886
1116	13	-2.9576837232459354
1116	14	-2.7443235842837694
1116	15	-2.27119872147604
1116	16	-2.346907275751928
1116	17	-2.833314093311081
1116	18	-2.6183405099343737
1116	19	-2.819909023876729
1116	20	-3.388599566806022
1116	21	-3.8804528948947414
1116	22	-2.9374060957430643
1116	23	-3.000569443568441
1116	24	-3.6354771660141516
1116	25	-3.245496770202923
1201	1	0.008612318925088402
1201	2	0.014941207355061437
1201	3	0.29074953152224836
1201	4	0.28770670951712063
1201	5	0.36619570406012514
1201	6	0.2681086081660311
1201	7	0.25929499896207986
1201	8	-0.2839720281738529
1201	9	-0.10048685317580208
1201	10	0.19318568974403405
1201	11	-0.12155165248516653
1201	12	-0.5520995541873717
1201	13	-0.20575393633640005
1201	14	0.28420314692521487
1201	15	-0.09331223859761195
1201	16	0.395987608575183
1201	17	0.46581827231185713
1201	18	0.8581324457672466
1201	19	0.2970320745240187
1201	20	0.34696227016871717
1201	21	0.2733176498106573
1201	22	0.5583458917250468
1201	23	0.711127966052004
1201	24	0.4904518066031258
1201	25	0.5369107755373577
1202	1	0.6008292656709493
1202	2	0.7091536787502157
1202	3	0.367391074525127
1202	4	0.8739231364630449
1202	5	0.6339670512505577
1202	6	0.6823692984011913
1202	7	0.851082582505132
1202	8	0.934589055714671
1202	9	1.1602439517322907
1202	10	1.268007932955463
1202	11	0.8515229219022231
1202	12	1.0900334004949457
1202	13	0.9277349143774813
1202	14	1.160609512150181
1202	15	0.7267750708883156
1202	16	0.9982464140072977
1202	17	1.0834857651646104
1202	18	1.4974022747615194
1202	19	1.3269478288995344
1202	20	1.163290116020569
1202	21	1.0817231951885589
1202	22	1.2698288217908242
1202	23	1.2424995828622514
1202	24	1.483328645712369
1202	25	1.3384663719443992
1203	1	-0.08226583061652093
1203	2	0.1747408338028258
1203	3	0.3684718269571299
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2211	20	0.3434567929253731
2211	21	0.5355901403326087
2211	22	0.4132119124802571
2211	23	0.9505058076508348
2211	24	0.7620865546748377
2211	25	0.9141036961043625
2212	1	-0.1891834070705869
2212	2	-0.2341327855548947
2212	3	-0.20145610830431337
2212	4	-0.2632924811822406
2212	5	-0.21444080987228276
2212	6	-0.2830964751389331
2212	7	-0.3334345458114072
2212	8	-0.20059490019223247
2212	9	-0.08265227259534669
2212	10	-0.08603628083167081
2212	11	-0.21807859078057845
2212	12	0.04698228710408614
2212	13	-0.19800483176538108
2212	14	-0.13938847630354445
2212	15	-0.32203711103593236
2212	16	0.05294035724146795
2212	17	-0.16707847356570227
2212	18	-3.1691939877855013
2212	19	-1.2370072733875972
2212	20	-0.42810046591976914
2212	21	-0.587771705541634
2212	22	-0.19691629518059273
2212	23	-0.3078494204165736
2212	24	-0.6808738767666611
2212	25	-0.2935413523932979
2213	1	0.47129136283364303
2213	2	0.6340224362387588
2213	3	0.15485767201927203
2213	4	0.25430284420913196
2213	5	0.4583087799872132
2213	6	0.44748987681524
2213	7	0.6949289042150077
2213	8	0.32827541596352816
2213	9	0.432457184271378
2213	10	0.2910069735984422
2213	11	0.35321435480281593
2213	12	0.22380070906616822
2213	13	0.4265400413790559
2213	14	0.519469565429489
2213	15	0.24197737993303292
2213	16	-0.25551628306233454
2213	17	0.18829943167893504
2213	18	0.42943080019271207
2213	19	0.5106663461370786
2213	20	-0.3719487556438139
2213	21	0.12301548615783986
2213	22	0.32448211510291003
2213	23	-0.3099360722797826
2213	24	0.04295042023464646
2213	25	-0.09094306763463322
2214	1	-1.4380252704346326
2214	2	-3.6413928161671834
2214	3	-1.7304932628030905
2214	4	-1.2476980219216003
2214	5	-1.0289587511323077
2214	6	-1.3629385801789375
2214	7	-1.4239917996941216
2214	8	-1.130755211911822
2214	9	-1.3133247677512294
2214	10	-1.5173854686732504
2214	11	-1.091694783917827
2214	12	-1.8174659073221058
2214	13	-1.450705454610965
2214	14	-2.7623002052194394
2214	15	-2.9723947490562246
2214	16	-2.0463240150582287
2214	17	-2.5978938543325363
2214	18	-1.8240400147599303
2214	19	-4.160035176361056
2214	20	-2.4648911787790517
2214	21	-2.2678173271788395
2214	22	-2.2686139203963016
2214	23	-2.425349727811433
2214	24	-2.6634299604289424
2214	25	-2.3815911122013134
2215	1	-0.7464842068652899
2215	2	-2.723104600276251
2215	3	-1.2723127868039512
2215	4	-0.732919528784997
2215	5	-0.7263778527703622
2215	6	-1.2183045500715686
2215	7	-1.7140812247387416
2215	8	-1.87310035333795
2215	9	-1.5238053456033462
2215	10	-2.8063946915070055
2215	11	-1.876696699501334
2215	12	-2.2055710681843976
2215	13	-1.484669217377629
2215	14	-2.8447945752727932
2215	15	-2.6939502028626308
2215	16	-2.2719003253640686
2215	17	-2.488531790361389
2215	18	-1.098698713845124
2215	19	-1.922486936361807
2215	20	-2.05115551061418
2215	21	-1.736796554778195
2215	22	-1.3443308625643695
2215	23	-1.9878524143004945
2215	24	-1.6489761820572824
2215	25	-1.5678110585649776
2216	1	-2.3966136567034795
2216	2	-2.305295398081231
2216	3	-2.852307158550115
2216	4	-3.8241352316131945
2216	5	-2.7519610805739383
2216	6	-2.699461156234509
2216	7	-2.59810542623023
2216	8	-3.303139751114788
2216	9	-3.627407428057264
2216	10	-3.022271995573419
2216	11	-3.693456232356265
2216	12	-4.078860782550514
2216	13	-4.277268063585591
2216	14	-3.6866884826880195
2216	15	-3.7861691419367816
2216	16	-2.9072767148392344
2216	17	-3.122760016054368
2216	18	-3.1997666750101743
2216	19	-3.6217637057441046
2216	20	-3.3900988059568
2216	21	-3.778455482595838
2216	22	-4.3983030027078875
2216	23	-4.286370776478627
2216	24	-4.0975845962967625
2216	25	-4.434390487297211
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83741.0
3	1.0
4	728.0
5	4093.0
6	5193.0
7	4516.0
8	4177.0
9	3831.0
10	3373.0
11	3318.0
12	3242.0
13	2997.0
14	3010.0
15	3107.0
16	3154.0
17	3247.0
18	3755.0
19	4663.0
20	5817.0
21	7594.0
22	9446.0
23	11263.0
24	12914.0
25	14484.0
26	16094.0
27	17821.0
28	20326.0
29	23765.0
30	28848.0
31	35824.0
32	44480.0
33	56252.0
34	71585.0
35	91791.0
36	123543.0
37	187483.0
38	471917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67793541686201	18.495849051160175	12.220018320434498	25.606197211543318
2	14.771048319664558	20.278197375336386	40.606866285661695	24.343888019337356
3	18.701539032265256	25.043369982436325	29.43601408832451	26.819076896973915
4	12.32129120385348	16.95201121535641	37.81796613825084	32.90873144253928
5	12.969014335641962	38.098771343670755	35.89000833291992	13.04220598776736
6	31.99848757895463	36.751040688007805	18.3065484812173	12.94392325182026
7	27.686458642579215	31.6385041760324	22.70253523516105	17.972501946227336
8	25.300827198416197	36.43518961153232	20.86377428776613	17.400208902285343
9	26.79146380123513	14.931590649488072	19.95412299953059	38.32282254974621
10	14.892862996328127	28.9071508055403	34.840300880731725	21.359685317399844
11	33.8314440922909	22.646379939085186	24.506028582663074	19.01614738596084
12	23.309846195428708	25.03982414119311	31.590535943803395	20.059793719574788
13	28.957704644164927	21.921585597812413	25.430839279373053	23.689870478649606
14	20.942323314378555	22.238341372939477	27.529312811369994	29.29002250131198
15	23.819711635236406	30.14889835918494	23.167742139558424	22.86364786602023
16	22.233723886728733	27.892327167722303	27.696726985708466	22.1772219598405
17	22.238133029686118	28.491296021531348	27.581863979848865	21.68870696893367
18	22.405067752997397	27.216368623495907	29.02570491963661	21.35285870387009
19	23.40506749519432	27.366069952309818	27.493670303696028	21.735192248799837
20	23.760319290567644	27.326315305186462	27.601208841450266	21.312156562795632
21	22.86506902689475	26.98871070240365	27.258003295347695	22.888216975353906
22	23.226072192086676	27.9387429715007	26.555183513739273	22.280001322673353
23	22.18774890517665	27.90771566510339	27.351030701060747	22.553504728659213
24	22.930823170841286	27.44782175833576	27.672528744307744	21.94882632651521
25	22.660670422466364	27.101351239636745	27.26561056665514	22.97236777124175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	165.0
1	165.0
2	273.0
3	381.0
4	381.0
5	381.0
6	785.5
7	1190.0
8	1190.0
9	1190.0
10	1618.5
11	2047.0
12	2047.0
13	2047.0
14	3721.0
15	5395.0
16	5395.0
17	5395.0
18	10036.0
19	14677.0
20	14677.0
21	14677.0
22	23729.5
23	32782.0
24	32782.0
25	32782.0
26	46717.5
27	60653.0
28	60653.0
29	60653.0
30	78614.0
31	96575.0
32	96575.0
33	96575.0
34	112446.5
35	128318.0
36	128318.0
37	128318.0
38	140691.5
39	153065.0
40	153065.0
41	153065.0
42	162805.0
43	172545.0
44	172545.0
45	172545.0
46	185379.5
47	198214.0
48	198214.0
49	198214.0
50	188339.0
51	178464.0
52	178464.0
53	178464.0
54	157108.5
55	135753.0
56	135753.0
57	135753.0
58	115747.5
59	95742.0
60	95742.0
61	95742.0
62	77775.5
63	59809.0
64	59809.0
65	59809.0
66	46021.5
67	32234.0
68	32234.0
69	32234.0
70	23432.5
71	14631.0
72	14631.0
73	14631.0
74	10217.0
75	5803.0
76	5803.0
77	5803.0
78	3911.0
79	2019.0
80	2019.0
81	2019.0
82	1347.5
83	676.0
84	676.0
85	676.0
86	431.0
87	186.0
88	186.0
89	186.0
90	113.5
91	41.0
92	41.0
93	41.0
94	29.0
95	17.0
96	17.0
97	17.0
98	14.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35669289697447093
2	0.0366539144583881
3	0.032629170910016075
4	0.031838596284443
5	0.03766010034548111
6	0.017680123444634262
7	0.017248900921594403
8	0.022423571198072725
9	0.020267458582873422
10	0.019908106480340207
11	0.019405013536793702
12	0.019908106480340207
13	0.019476883957300347
14	0.026088962643911535
15	0.027095148531004542
16	0.020842421946926572
17	0.02192047825452622
18	0.09127543404343705
19	0.023789109187698946
20	0.022207959936552793
21	0.024507813392765376
22	0.019476883957300347
23	0.021632996572499646
24	0.01782386428564755
25	0.021561126151993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1391393.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.49318527279875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.69786865079324	61.512229676911225
2	11.403766577442005	16.761982597673907
3	2.807619476424472	6.190226950691459
4	0.9453203598407589	2.778984173917028
5	0.4104651995204017	1.5083197478194597
6	0.22331176983334147	0.9847135964375016
7	0.11912379078321625	0.612835077850032
8	0.08184138250394349	0.4811827109875448
9	0.05360492029284705	0.3545636704754236
>10	0.22717998663712513	2.850248711730261
>50	0.014396852971549417	0.7461184953072708
>100	0.01216546447039715	1.9671523948221767
>500	0.0017658891988366684	0.8595095443295574
>1k	0.001373469376872964	1.6043793625049336
>5k	1.96209910981852E-4	0.7875532885422158
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5807	0.4173515318820779	No Hit
CCCATGTACTCTGCGTTGATACCAC	5116	0.36768907131198736	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2351	0.1689673586111185	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2043	0.14683126909507235	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1913	0.13748811442920872	No Hit
GTCCTACAGTGGACATTTCTAAATT	1826	0.13123538784513075	No Hit
CATGTACTCTGCGTTGATACCACTG	1689	0.1213891402357206	No Hit
GCGTTGATACCACTGCTTCCCATGT	1641	0.11793936005140174	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1590	0.11427396860556292	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1581	0.11362713482100312	No Hit
GTATCAACGCAGAGTACATGGGAAG	1531	0.11003361379567096	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.187042050664334E-5	0.0
3	0.0	0.0	0.0	7.187042050664334E-5	0.0
4	0.0	0.0	0.0	7.187042050664334E-5	0.0
5	0.0	0.0	0.0	7.187042050664334E-5	0.0
6	0.0	0.0	0.0	7.187042050664334E-5	0.0
7	0.0	0.0	0.0	7.187042050664334E-5	0.0
8	0.0	0.0	0.0	7.187042050664334E-5	0.0
9	0.0	0.0	0.0	7.187042050664334E-5	0.0
10	0.0	0.0	0.0	7.187042050664334E-5	0.0
11	0.0	0.0	0.0	7.187042050664334E-5	0.0
12	0.0	0.0	0.0	7.187042050664334E-5	0.0
13	0.0	0.0	0.0	7.187042050664334E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTAC	65	8.0312835E-4	11.690781	3
AGTCGTC	75	2.0829766E-4	11.394414	8
ATAGGAC	165	8.0035534E-11	10.937965	3
TGGCGAG	960	0.0	10.887518	18
CAAGTCG	70	0.0014973412	10.851823	6
GGACCGT	70	0.0014973412	10.851823	6
CACCGCC	70	0.0014973412	10.851823	7
GTATAAT	195	1.8189894E-12	10.7463045	1
GTATAGA	205	0.0	10.686736	1
AGGACCG	80	3.7759432E-4	10.68572	5
TTAGGAC	645	0.0	10.603267	3
GGCGAGA	540	0.0	10.550384	19
GTCCTAC	1005	0.0	10.520296	1
GTATAAA	200	1.8189894E-12	10.477647	1
AGGACGT	935	0.0	10.463497	5
GGACGTG	970	0.0	10.278466	6
GTGCTAG	140	9.313226E-8	10.2055	1
ATGGCGA	1055	0.0	10.1773205	17
TATGTCG	75	0.002643034	10.13529	16
GTCCTAA	635	0.0	10.05014	1
>>END_MODULE