FastQCFastQC Report
Thu 26 May 2016
SRR1512998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391393
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC59290.42611972318388835No Hit
GTACATGGGAAGCAGTGGTATCAAC58170.41807023608714433No Hit
GTATCAACGCAGAGTACTTTTTTTT24000.172489009215944No Hit
GAGTACATGGGAAGCAGTGGTATCA21220.15250903231509716No Hit
TATCAACGCAGAGTACTTTTTTTTT19210.13806307779326185No Hit
CATGTACTCTGCGTTGATACCACTG18400.13224157373222375No Hit
GTCCTAAAGTGTGTATTTCTCATTT17430.12527014294307934No Hit
CTTTAGGACGTGAAATATGGCGAGG17350.1246951795790262No Hit
GTCCTACAGTGGACATTTCTAAATT17200.12361712327142654No Hit
CTGTAGGACGTGGAATATGGCAAGA17090.12282654864585348No Hit
GGTATCAACGCAGAGTACTTTTTTT16600.11930489804102794No Hit
GCGTTGATACCACTGCTTCCCATGT15980.11484893196961606No Hit
TATCAACGCAGAGTACATGGGAAGC15330.11017735463668425No Hit
GTATCAACGCAGAGTACATGGGAAG15300.10996174337516432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGA456.7110936E-414.7924246
CCAGTAC2450.012.4155443
TAGGACC3450.012.4080524
GCCCTAT550.003054531812.0981011
GTCCTAA5400.011.7940481
ACACCGT657.956086E-411.7038966
GGCGAGG4400.011.64841719
CTTGGAC1658.0035534E-1110.9459013
TAGGACA4150.010.7735794
TGGCGAG9550.010.7336218
GATCTAT803.7494738E-410.6938571
CTGTGCG909.486417E-510.5584149
CTAGGAC1909.094947E-1210.5062463
TTGGACA1553.5779522E-910.4334384
CTAAGAC1855.820766E-1110.2763793
CTTAGGC1303.8073995E-710.2368553
CTATAGG1409.5962605E-810.1846261
GTACTAA951.6337054E-410.0059481
ATAGTAC951.6337054E-410.0059483
TGCGTTG13750.09.94212612
Produced by FastQC (version 0.11.5)