##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512998_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1391393
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.501365178637524	31.0	31.0	34.0	27.0	34.0
2	30.61070308676269	31.0	31.0	34.0	27.0	34.0
3	30.72554986261969	31.0	31.0	34.0	27.0	34.0
4	34.21939092693437	37.0	35.0	37.0	30.0	37.0
5	33.90794333448566	37.0	35.0	37.0	30.0	37.0
6	33.77686678026984	37.0	35.0	37.0	29.0	37.0
7	33.64235769477064	37.0	35.0	37.0	28.0	37.0
8	33.52783361710171	37.0	35.0	37.0	28.0	37.0
9	34.98019251210837	39.0	35.0	39.0	28.0	39.0
10	34.87016608535475	39.0	35.0	39.0	27.0	39.0
11	34.92202059375029	39.0	35.0	39.0	27.0	39.0
12	34.76865270991014	39.0	35.0	39.0	27.0	39.0
13	34.762706870021624	39.0	35.0	39.0	27.0	39.0
14	35.823984309249795	40.0	36.0	41.0	27.0	41.0
15	35.8404821642771	40.0	36.0	41.0	27.0	41.0
16	35.79767901664016	40.0	36.0	41.0	27.0	41.0
17	35.773562897039156	40.0	36.0	41.0	27.0	41.0
18	35.69933728285251	40.0	36.0	41.0	27.0	41.0
19	35.67434218800871	40.0	36.0	41.0	27.0	41.0
20	35.663404228711805	40.0	36.0	41.0	27.0	41.0
21	35.605175532721525	40.0	36.0	41.0	27.0	41.0
22	35.580726653073576	40.0	36.0	41.0	27.0	41.0
23	35.48281470440055	40.0	36.0	41.0	27.0	41.0
24	35.46201540470593	40.0	36.0	41.0	27.0	41.0
25	35.368471021487096	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5603378673355195
1101	2	0.5721601098809188
1101	3	0.5859819823126209
1101	4	0.4233475994315299
1101	5	0.6505422870382276
1101	6	0.5560712283750462
1101	7	0.6910692467172765
1101	8	0.6542626969690488
1101	9	0.5259545652350539
1101	10	0.798036318791091
1101	11	0.8426338890952678
1101	12	0.9600631329226985
1101	13	0.959220432788392
1101	14	1.192070026467377
1101	15	1.0002949353908122
1101	16	1.2197591708851192
1101	17	1.2412772546920294
1101	18	0.6354876097867574
1101	19	0.9118791711407326
1101	20	0.9674752866584342
1101	21	0.9989796803563848
1101	22	0.9768850085477467
1101	23	1.0185226126897575
1101	24	0.8795256028326293
1101	25	0.9480451153377771
1102	1	0.8656638557299345
1102	2	0.7654831808552096
1102	3	0.6163084472385378
1102	4	0.7281358927177166
1102	5	0.8680237248269833
1102	6	0.6470504136884117
1102	7	0.6780393044100563
1102	8	0.6966264524227128
1102	9	0.8457222859754481
1102	10	0.9215277300176723
1102	11	0.9251306041698584
1102	12	0.9100786437613309
1102	13	1.0140045891527265
1102	14	1.0602752370618305
1102	15	1.0641855564338982
1102	16	1.0750437795878156
1102	17	0.9661252411421941
1102	18	1.095025686329656
1102	19	1.2356053500639703
1102	20	1.043873026872724
1102	21	1.159014669289327
1102	22	1.2289211595891771
1102	23	1.2224240546636196
1102	24	1.1289176847851579
1102	25	1.2011941507228059
1103	1	0.6526381332226734
1103	2	0.4709675955538657
1103	3	0.632529489158177
1103	4	0.6360304601351388
1103	5	0.6193636239709974
1103	6	0.633858771168164
1103	7	0.6973960261020267
1103	8	0.636936533586173
1103	9	0.8234138313104609
1103	10	0.9408063765125974
1103	11	0.8396262046733796
1103	12	0.909059169722461
1103	13	0.8621444475831836
1103	14	0.9990998456281517
1103	15	0.6815151026432744
1103	16	0.8207405996994979
1103	17	1.0263490144674137
1103	18	1.1409261355481846
1103	19	1.102498584684632
1103	20	1.1495153088022647
1103	21	0.6914327378520255
1103	22	0.9861475606966152
1103	23	0.9405862871564281
1103	24	0.968123451558192
1103	25	0.8909633771999452
1104	1	0.4692461074462315
1104	2	0.6231616205243924
1104	3	0.4923676507920298
1104	4	0.5451480445473749
1104	5	0.5583304887760221
1104	6	0.6800620443917609
1104	7	0.6975815408367083
1104	8	0.5741656628753944
1104	9	0.8417165371984723
1104	10	0.7287335302517661
1104	11	0.8834945789378423
1104	12	0.7727135189921412
1104	13	0.828288816847234
1104	14	1.008430953862458
1104	15	1.1019866738237454
1104	16	1.0236136934749709
1104	17	0.9141144187581816
1104	18	0.9887476711817627
1104	19	1.027073709462492
1104	20	1.0953526855349978
1104	21	0.93357776510733
1104	22	0.9894759132893967
1104	23	0.899046951363843
1104	24	1.003996908683881
1104	25	1.0668992464941098
1105	1	0.47912373950473963
1105	2	0.6023858516723379
1105	3	0.5191081771936652
1105	4	0.43043992944055987
1105	5	0.4252629538346113
1105	6	0.6156655376269953
1105	7	0.6498526068694801
1105	8	0.7461817235418593
1105	9	0.821682386132359
1105	10	0.7900061004618095
1105	11	0.7078316435167835
1105	12	0.7726547211660488
1105	13	0.8324189614053097
1105	14	1.0528027735947703
1105	15	0.959519339842025
1105	16	0.9140495827014803
1105	17	0.7418820948546525
1105	18	1.1211609796267012
1105	19	0.9640424398925802
1105	20	1.0636803907887469
1105	21	0.9873280394636268
1105	22	1.0766026125365045
1105	23	0.9514501111429112
1105	24	1.1167868453956729
1105	25	1.1949959487817878
1106	1	0.3197132065772905
1106	2	0.3199944313043588
1106	3	0.28313371096090734
1106	4	0.29063529873702976
1106	5	0.3237416811613656
1106	6	0.40729918392090525
1106	7	0.46826291817035326
1106	8	0.4718938852755983
1106	9	0.3455776074458825
1106	10	0.5791378510037006
1106	11	0.3870795442757782
1106	12	0.6902267274431466
1106	13	0.5239350362892594
1106	14	0.7699031028543502
1106	15	0.7215055115360371
1106	16	0.7508324393389003
1106	17	0.6325903756386282
1106	18	0.8115057133120516
1106	19	0.695857780228522
1106	20	0.8507489270694109
1106	21	0.7567272724170166
1106	22	0.8606482815257763
1106	23	0.9533992324382439
1106	24	0.9124984207631215
1106	25	0.7876930051236357
1107	1	0.5331864637460129
1107	2	0.5627529030400922
1107	3	0.5091297043558782
1107	4	0.5525655919186079
1107	5	0.6453410327439784
1107	6	0.6670741286629394
1107	7	0.6676957089011637
1107	8	0.7253786121650592
1107	9	0.595176196459704
1107	10	0.8507445027399783
1107	11	0.7719888693497694
1107	12	0.7078237039904565
1107	13	0.8797675276845283
1107	14	1.0233075183069715
1107	15	0.9545324593369173
1107	16	0.8738543559791339
1107	17	0.9400861032456262
1107	18	1.1000580782207123
1107	19	1.1045075169064233
1107	20	1.2390428334001555
1107	21	1.0341791152818445
1107	22	0.978725781672459
1107	23	0.9609128351878553
1107	24	1.0779117156977094
1107	25	0.9112938658595127
1108	1	0.5157940125643954
1108	2	0.4276423307186086
1108	3	0.43840589812921493
1108	4	0.5275398617107356
1108	5	0.5155775896328976
1108	6	0.5750428924110338
1108	7	0.3572861744717599
1108	8	0.477265605897216
1108	9	0.3959386042282418
1108	10	0.667412194972762
1108	11	0.6268273656896497
1108	12	0.6981829990987336
1108	13	0.5308283368542774
1108	14	0.5848149356420294
1108	15	0.5013625878240546
1108	16	0.4841212121167473
1108	17	0.4943164101237656
1108	18	0.610189042055417
1108	19	0.5519378603598923
1108	20	0.7177686623882522
1108	21	0.7879930231468961
1108	22	0.897154361343901
1108	23	0.8313355275346979
1108	24	0.7028485762883818
1108	25	0.7723413503375198
1109	1	0.14471329606303485
1109	2	0.09843170796285605
1109	3	0.20227677957420198
1109	4	0.2538984182418602
1109	5	0.15547010425950702
1109	6	-0.0569779084844555
1109	7	0.13026214103638267
1109	8	0.44368121783286085
1109	9	0.39183994703274294
1109	10	0.24819948759056132
1109	11	0.29358066364299873
1109	12	0.30599025758811393
1109	13	0.38010241360530017
1109	14	0.439911299748438
1109	15	0.3667420661042442
1109	16	0.39509260501876753
1109	17	0.4614158812231395
1109	18	0.5261529784089944
1109	19	0.7225664906116904
1109	20	0.642751248130736
1109	21	0.5940895190580164
1109	22	0.7071171939796841
1109	23	0.5769428519655477
1109	24	0.19937002701630036
1109	25	0.39888580235649584
1110	1	0.08776867398859878
1110	2	0.16301520905784628
1110	3	0.1361471081028398
1110	4	0.2095374993658723
1110	5	0.05915557128427196
1110	6	0.21823173437663712
1110	7	0.17465345437726398
1110	8	0.15135909650447132
1110	9	0.2814902764771645
1110	10	0.3270727292510358
1110	11	0.1568663605604499
1110	12	0.43290056944321975
1110	13	0.011731367451496055
1110	14	0.39918074312710417
1110	15	0.4862945490557138
1110	16	0.40635395162777144
1110	17	0.2734209843353739
1110	18	0.04363887458782045
1110	19	0.330484209242627
1110	20	0.4343762704331695
1110	21	0.5791843892618118
1110	22	0.4122304903344016
1110	23	0.5345295472424851
1110	24	0.4861883817994439
1110	25	0.47446341560942784
1111	1	-0.29093967836192647
1111	2	-0.019556873141496567
1111	3	-0.030074415413675837
1111	4	-0.01699246891063666
1111	5	0.3350950205464045
1111	6	0.16283113371511604
1111	7	0.3022193665544677
1111	8	0.5514808065303356
1111	9	0.186450805239609
1111	10	0.24987701840250054
1111	11	0.29422401336005777
1111	12	0.2672918220985778
1111	13	0.30210979547636185
1111	14	0.5015566242569776
1111	15	0.6570844245846672
1111	16	0.4957953251479523
1111	17	0.7602723572357348
1111	18	0.6711808391904839
1111	19	0.749930203236012
1111	20	0.6927762343709389
1111	21	0.4493209186102689
1111	22	0.3833398546812532
1111	23	0.3204297788296131
1111	24	0.4826455494493729
1111	25	0.5658572207945056
1112	1	0.21798456944974376
1112	2	-0.04129969966860969
1112	3	0.13485611528143338
1112	4	0.010654073718562529
1112	5	0.1906550557444291
1112	6	0.23749180415961035
1112	7	0.20284545653450436
1112	8	0.32373878633886477
1112	9	-0.01017609793335339
1112	10	0.3468276861101316
1112	11	0.11933287774783707
1112	12	0.25510965962255483
1112	13	0.31554078994555823
1112	14	0.24174995522790965
1112	15	0.26946367340173794
1112	16	0.005873209749182706
1112	17	0.22386291459760344
1112	18	0.20509402963554635
1112	19	0.08989466234876886
1112	20	0.4790404135654214
1112	21	0.5091379945726118
1112	22	0.4045088880848908
1112	23	0.5560360926111585
1112	24	0.6237496484297935
1112	25	0.6308253253591758
1113	1	-0.1652489825122032
1113	2	-0.6400121575275719
1113	3	-0.20314504654511722
1113	4	-0.3164982099657223
1113	5	-0.0458257021239632
1113	6	-0.325392703330202
1113	7	-0.0214512163716023
1113	8	0.04512046622089372
1113	9	0.06047785153215557
1113	10	-0.15514434068139593
1113	11	-0.03300740833953597
1113	12	-0.09129319628613075
1113	13	-0.04207617470810021
1113	14	0.005613773267995725
1113	15	0.03563426330179453
1113	16	-0.21291620670605482
1113	17	0.040481999336464014
1113	18	0.10970985728801708
1113	19	-0.07666439468500386
1113	20	0.20140756068543197
1113	21	-0.05264883508077389
1113	22	-0.28218581468115644
1113	23	-0.01731041470983996
1113	24	-0.1529172995908823
1113	25	-0.2562852517309935
1114	1	-0.6903698541499281
1114	2	-0.6531296449657944
1114	3	-0.61435211209335
1114	4	-0.37279374963204504
1114	5	-0.44199227077265846
1114	6	-0.8926315886078982
1114	7	-0.7503359781879269
1114	8	-0.9883496795679392
1114	9	-0.8272049003998347
1114	10	-1.1276111951298589
1114	11	-0.907820916248042
1114	12	-0.9367665199208446
1114	13	-0.7824040706233362
1114	14	-1.8964049842768063
1114	15	-1.0441870344561721
1114	16	-1.1061265532769724
1114	17	-1.3288280573076108
1114	18	-1.4973554845883612
1114	19	-1.406051421232398
1114	20	-0.7413127991952919
1114	21	-0.7404987334516164
1114	22	-0.9773616795608007
1114	23	-0.7845486809266546
1114	24	-1.5733062761800625
1114	25	-1.4657358351958791
1115	1	-1.8887086639345227
1115	2	-1.5424707559662707
1115	3	-1.1393888983568594
1115	4	-2.2772296751523093
1115	5	-1.5367279628522752
1115	6	-1.4483738413654876
1115	7	-1.247697324399219
1115	8	-1.1539335024628272
1115	9	-1.615629950462342
1115	10	-1.5138199419629217
1115	11	-1.38509779234748
1115	12	-1.9700179830737312
1115	13	-1.3656060244441264
1115	14	-2.395235015144081
1115	15	-2.478862374936554
1115	16	-1.7536325552462841
1115	17	-1.8246087005417024
1115	18	-1.6952213281278077
1115	19	-1.4326025292457132
1115	20	-1.4686671200600188
1115	21	-1.6106888603503933
1115	22	-1.743976347599343
1115	23	-2.3149955372223587
1115	24	-1.6401051521246117
1115	25	-1.4314831218632094
1116	1	-1.4086418497941864
1116	2	-1.4225635583943976
1116	3	-1.3830868795009756
1116	4	-1.5812142828701
1116	5	-1.379689646758024
1116	6	-2.134586124523949
1116	7	-1.4654509892551602
1116	8	-1.6894740362994654
1116	9	-1.8861649258495703
1116	10	-2.011101037146581
1116	11	-1.8049459102542542
1116	12	-2.5793379129899137
1116	13	-2.2370510972677025
1116	14	-2.758312817651131
1116	15	-2.366730523555738
1116	16	-2.6967585121902857
1116	17	-2.7033344401086126
1116	18	-3.5467419530192856
1116	19	-2.7589226891667025
1116	20	-2.6255334962892505
1116	21	-2.640782001020881
1116	22	-3.9275596420348045
1116	23	-3.7654193548962027
1116	24	-2.7698859259780235
1116	25	-3.3623148147211097
1201	1	0.6533963595838941
1201	2	0.5655125423356822
1201	3	0.5768132540751729
1201	4	0.6312097279947508
1201	5	0.5982964011469889
1201	6	0.6366169779971074
1201	7	0.729159663942859
1201	8	0.6674341058696243
1201	9	0.8569287247754005
1201	10	0.7968075750463441
1201	11	0.7789491868697098
1201	12	0.544934933637073
1201	13	0.8050416286392874
1201	14	0.8793798719842627
1201	15	0.8856795502839958
1201	16	0.7826790493395777
1201	17	0.7550461056334825
1201	18	0.7850468059541029
1201	19	0.7077545956908651
1201	20	0.8025410350964677
1201	21	0.7464121489621931
1201	22	0.8823168864195665
1201	23	0.8736888625702406
1201	24	0.8522432326221505
1201	25	0.7582791665314588
1202	1	0.7666662604204326
1202	2	0.8437054557298289
1202	3	0.8044687239130432
1202	4	0.8191531162874242
1202	5	0.8776487219692086
1202	6	0.8732702693238465
1202	7	0.7994281638551683
1202	8	0.7222658153563941
1202	9	0.9650338004316481
1202	10	1.0092669260571014
1202	11	0.945433923557232
1202	12	0.9622535688875402
1202	13	0.9906917694712618
1202	14	1.0820357115985075
1202	15	1.1545424773493025
1202	16	0.9465861277046983
1202	17	1.087421612491255
1202	18	1.0055653803499425
1202	19	1.1619524028112806
1202	20	1.223038056811852
1202	21	1.2324109360956612
1202	22	1.203374700428384
1202	23	1.1358269307316462
1202	24	1.056552712209438
1202	25	1.0982137478072502
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2211	7	0.4001550717323923
2211	8	0.3915043025689613
2211	9	0.028256357099742502
2211	10	0.10301961356861966
2211	11	0.27052965015796815
2211	12	0.28357756503800147
2211	13	0.2152100650258646
2211	14	-0.748989945178117
2211	15	-0.05093902233331704
2211	16	0.24286149269435953
2211	17	0.4581118678304392
2211	18	0.10984287487253397
2211	19	0.05135797645421292
2211	20	-0.030390695978042004
2211	21	-0.029028136738453725
2211	22	0.1536644397653646
2211	23	0.1446133017208524
2211	24	-4.982941996374279E-4
2211	25	-0.03933155595833426
2212	1	-0.27129362934683243
2212	2	-0.22396899562092543
2212	3	-0.039901963834630294
2212	4	-0.02912801742005655
2212	5	-0.28350228480970685
2212	6	-0.16187144411127008
2212	7	-0.058174475293540695
2212	8	-0.32187354456958417
2212	9	-0.4715421454979918
2212	10	-0.48010004032995823
2212	11	-0.26473041977348544
2212	12	-0.18792042986530078
2212	13	-0.18184056613512212
2212	14	-0.2331072087643733
2212	15	-0.22979562334870707
2212	16	-0.2598508203772951
2212	17	-0.30188299596126456
2212	18	-0.0040920533193187225
2212	19	-0.08000845249819832
2212	20	-0.3682801094625674
2212	21	-0.10063675766652835
2212	22	-0.12953620818706924
2212	23	-0.5099055738446197
2212	24	-0.4669924369467182
2212	25	-0.42665499879826285
2213	1	0.13230686798576485
2213	2	0.011235955460140445
2213	3	0.09321871876627696
2213	4	0.12399560468661264
2213	5	0.22319224095664936
2213	6	0.4562188304941799
2213	7	-0.11795925580382516
2213	8	0.21130155861104072
2213	9	0.16408267823096878
2213	10	0.3279990925158387
2213	11	0.2811302397086095
2213	12	0.17617906843256748
2213	13	0.3053324635022463
2213	14	0.39347347073813665
2213	15	0.35830414506590813
2213	16	0.11831103516585983
2213	17	0.14695609787649744
2213	18	-0.05927809622816227
2213	19	-0.2693992200667097
2213	20	0.20883504368966044
2213	21	-0.4555991597988651
2213	22	-0.040312277432292376
2213	23	0.1196030917389308
2213	24	-0.25681817192246825
2213	25	-0.3084626359520257
2214	1	-0.7939513865091321
2214	2	-0.7729117692977461
2214	3	-0.5101558931889301
2214	4	-1.112782666714324
2214	5	-1.0105428566913446
2214	6	-2.5525813354418894
2214	7	-1.012861928919321
2214	8	-1.37336705959801
2214	9	-0.9285954795662477
2214	10	-1.2192180502768366
2214	11	-0.8405611278840439
2214	12	-0.9592801327310667
2214	13	-1.1861572379603658
2214	14	-1.231282767269036
2214	15	-1.5652715341914742
2214	16	-0.9814905705606662
2214	17	-1.3226794242449813
2214	18	-1.2945831643079444
2214	19	-3.1017860003775937
2214	20	-1.4699315618502808
2214	21	-1.8638231491900612
2214	22	-1.5613072689749288
2214	23	-1.692403146485205
2214	24	-0.7922314629502054
2214	25	-0.8276607099827729
2215	1	-1.3450329903664624
2215	2	-1.0577464217361445
2215	3	-1.1450835215096653
2215	4	-0.997183275963323
2215	5	-1.707996532275594
2215	6	-1.3304292474558679
2215	7	-1.6175709938342386
2215	8	-2.3040962895334403
2215	9	-1.9535125488169243
2215	10	-1.385379968476748
2215	11	-1.9433433961218043
2215	12	-2.6664093738796737
2215	13	-2.238529758214156
2215	14	-2.1583242653487815
2215	15	-3.031876372675079
2215	16	-2.4860622741217853
2215	17	-2.1873733513780564
2215	18	-3.1314497369085643
2215	19	-3.5913082258184126
2215	20	-2.9820712219042917
2215	21	-2.0377202870279802
2215	22	-3.126952914059359
2215	23	-2.9891387118626724
2215	24	-1.9560797300479535
2215	25	-2.2226068400368746
2216	1	-1.361568800131483
2216	2	-1.2075353622173601
2216	3	-1.7757814249851123
2216	4	-1.0848095599087273
2216	5	-1.0111342837881878
2216	6	-1.5046729508468886
2216	7	-3.163017736482331
2216	8	-2.313485623992424
2216	9	-2.069728955176849
2216	10	-2.085325093001863
2216	11	-2.6143198981620444
2216	12	-2.061832196448968
2216	13	-2.5967610794467433
2216	14	-2.792282334857717
2216	15	-2.7035873618406896
2216	16	-2.6711347378899006
2216	17	-3.1586256378561757
2216	18	-2.6727034304501416
2216	19	-2.880136141957429
2216	20	-3.3232236068768515
2216	21	-3.1224550555660358
2216	22	-3.3405085634191423
2216	23	-2.3958546257503883
2216	24	-2.635490170458816
2216	25	-2.4810238936459967
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33034.0
3	1.0
4	467.0
5	4476.0
6	5562.0
7	4272.0
8	3384.0
9	2943.0
10	2606.0
11	2429.0
12	2177.0
13	2120.0
14	2096.0
15	2148.0
16	2115.0
17	2250.0
18	2491.0
19	2986.0
20	3900.0
21	5039.0
22	6482.0
23	7861.0
24	9658.0
25	11091.0
26	12776.0
27	14189.0
28	16330.0
29	19677.0
30	24739.0
31	32032.0
32	40860.0
33	52395.0
34	67733.0
35	88060.0
36	119923.0
37	186347.0
38	594744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.79395368273269	19.343381999141236	13.204211549900135	27.658452768225946
2	13.728243831514883	20.778834357371455	39.414842886989746	26.078078924123915
3	16.89436413723513	25.177933193569324	30.072524441333254	27.855178227862293
4	11.554147756151952	17.62180791829651	37.17419576543461	33.64984856011692
5	12.584445184439863	38.33424793890099	36.121357969906484	12.959948906752667
6	32.03039197564384	37.23254363775895	17.901172855799942	12.835891530797264
7	27.418173190643436	31.661771736903216	22.716452356216678	18.203602716236666
8	25.291169157538825	36.76301459424041	20.509039855632746	17.436776392588015
9	26.323059886095614	14.733719726169245	19.870850831271934	39.07236955646321
10	14.427144381532944	29.189736815027956	34.94243345903641	21.440685344402688
11	33.82767691577903	22.45899636321819	24.46547932209237	19.2478473989104
12	22.97436693687807	25.121465740515102	31.6961066986406	20.20806062396622
13	28.941417806387804	21.66033195426357	25.56257708750816	23.83567315184047
14	20.788969209327266	22.090764091996093	27.539384129042364	29.580882569634277
15	23.618979711828324	30.26751405566489	23.129565126138	22.983941106368786
16	22.229826390136946	27.89842205528198	27.63308292297186	22.238668631609215
17	22.13595513696168	28.503630742684592	27.59026529585161	21.77014882450212
18	22.31749940167273	27.202868521085914	29.035582684516587	21.44404939272477
19	23.280580338051372	27.266702938992776	27.752066911660595	21.70064981129526
20	23.679103565755522	27.1445205528682	27.70198880175952	21.474387079616758
21	22.691017340907525	26.96709482302615	27.392927370575652	22.948960465490675
22	23.061144698674994	27.78448570779682	26.71080963757054	22.44355995595765
23	21.931905651386778	27.64740084217759	27.573589920317264	22.847103586118372
24	22.904815533785204	27.427261744165737	27.70504091942392	21.96288180262514
25	22.566704016939152	26.94789816672213	27.303959584082314	23.181438232256404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	128.0
2	223.0
3	318.0
4	318.0
5	318.0
6	764.5
7	1211.0
8	1211.0
9	1211.0
10	1554.0
11	1897.0
12	1897.0
13	1897.0
14	3595.5
15	5294.0
16	5294.0
17	5294.0
18	10183.5
19	15073.0
20	15073.0
21	15073.0
22	24118.0
23	33163.0
24	33163.0
25	33163.0
26	47672.5
27	62182.0
28	62182.0
29	62182.0
30	79376.5
31	96571.0
32	96571.0
33	96571.0
34	112367.5
35	128164.0
36	128164.0
37	128164.0
38	140672.5
39	153181.0
40	153181.0
41	153181.0
42	162870.0
43	172559.0
44	172559.0
45	172559.0
46	185942.5
47	199326.0
48	199326.0
49	199326.0
50	189815.5
51	180305.0
52	180305.0
53	180305.0
54	157437.5
55	134570.0
56	134570.0
57	134570.0
58	114922.0
59	95274.0
60	95274.0
61	95274.0
62	77188.0
63	59102.0
64	59102.0
65	59102.0
66	45213.5
67	31325.0
68	31325.0
69	31325.0
70	22558.5
71	13792.0
72	13792.0
73	13792.0
74	9561.5
75	5331.0
76	5331.0
77	5331.0
78	3593.0
79	1855.0
80	1855.0
81	1855.0
82	1225.5
83	596.0
84	596.0
85	596.0
86	366.0
87	136.0
88	136.0
89	136.0
90	84.5
91	33.0
92	33.0
93	33.0
94	20.0
95	7.0
96	7.0
97	7.0
98	3.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07373905143981607
2	4.3122252303986005E-4
3	0.0
4	0.029610613248737056
5	0.07143919798360347
6	0.07373905143981607
7	0.03579146941230839
8	0.07546394153197551
9	0.05411842664150244
10	0.11571137701569577
11	0.0038091322868520974
12	0.01868630933172727
13	0.008408839199277271
14	0.011499267281062936
15	0.009989988450423424
16	0.024651554233778666
17	0.03543211730977517
18	0.0010061858870930068
19	0.0047434477534384605
20	0.007402653312184264
21	7.187042050664334E-5
22	3.5935210253321674E-4
23	0.0
24	0.0
25	0.004312225230398601
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1391393.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.67639557970824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.1448192766655	58.87844561297919
2	12.448753442271387	17.84563552404997
3	3.0811897472480236	6.625457255396718
4	1.069113963504043	3.065209414716221
5	0.464268357115721	1.6638541209883835
6	0.24419145912246815	1.050165817274891
7	0.1280195713738492	0.6423187007815695
8	0.08446860835673613	0.4843524309315894
9	0.05789541529999674	0.3734761220364653
>10	0.24609928755670477	3.042211575259244
>50	0.014821822921388901	0.7616282127112192
>100	0.012846452844705314	2.0702290650482587
>500	0.0018064777985834547	0.8581047769659085
>1k	0.0015053981654862122	1.7939705786530966
>5k	2.0071975539816164E-4	0.844940792207357
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	5929	0.42611972318388835	No Hit
GTACATGGGAAGCAGTGGTATCAAC	5817	0.41807023608714433	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2400	0.172489009215944	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2122	0.15250903231509716	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1921	0.13806307779326185	No Hit
CATGTACTCTGCGTTGATACCACTG	1840	0.13224157373222375	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1743	0.12527014294307934	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1735	0.1246951795790262	No Hit
GTCCTACAGTGGACATTTCTAAATT	1720	0.12361712327142654	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1709	0.12282654864585348	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1660	0.11930489804102794	No Hit
GCGTTGATACCACTGCTTCCCATGT	1598	0.11484893196961606	No Hit
TATCAACGCAGAGTACATGGGAAGC	1533	0.11017735463668425	No Hit
GTATCAACGCAGAGTACATGGGAAG	1530	0.10996174337516432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATCGA	45	6.7110936E-4	14.792424	6
CCAGTAC	245	0.0	12.415544	3
TAGGACC	345	0.0	12.408052	4
GCCCTAT	55	0.0030545318	12.098101	1
GTCCTAA	540	0.0	11.794048	1
ACACCGT	65	7.956086E-4	11.703896	6
GGCGAGG	440	0.0	11.648417	19
CTTGGAC	165	8.0035534E-11	10.945901	3
TAGGACA	415	0.0	10.773579	4
TGGCGAG	955	0.0	10.73362	18
GATCTAT	80	3.7494738E-4	10.693857	1
CTGTGCG	90	9.486417E-5	10.558414	9
CTAGGAC	190	9.094947E-12	10.506246	3
TTGGACA	155	3.5779522E-9	10.433438	4
CTAAGAC	185	5.820766E-11	10.276379	3
CTTAGGC	130	3.8073995E-7	10.236855	3
CTATAGG	140	9.5962605E-8	10.184626	1
GTACTAA	95	1.6337054E-4	10.005948	1
ATAGTAC	95	1.6337054E-4	10.005948	3
TGCGTTG	1375	0.0	9.942126	12
>>END_MODULE