FastQCFastQC Report
Thu 26 May 2016
SRR1512997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212294
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC70850.3202558068683457No Hit
CCCATGTACTCTGCGTTGATACCAC59300.26804755606623715No Hit
GTATCAACGCAGAGTACTTTTTTTT31710.14333537947487993No Hit
GAGTACATGGGAAGCAGTGGTATCA29690.1342045858281042No Hit
GTCCTACAGTGGACATTTCTAAATT25750.11639501802201697No Hit
CTGTAGGACGTGGAATATGGCAAGA24350.11006674519751895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4700.012.1240654
GGACCGT855.3546348E-511.170386
GTAGGAC11650.010.5977163
ACGAAAT909.485871E-510.55911312
TGTAGGA12900.010.4542662
TGGACTG4100.010.19210155
CCGTCCC750.002658334810.1287289
ACCGCAT750.002658833310.1284998
CGAAATC951.6389534E-410.0033713
TAGGACA3800.09.9970374
TGGCGAG11450.09.87624318
AGGACGT11500.09.8275215
GTCTTAA1659.824362E-99.8118241
GGCGAGG5200.09.67785319
CTGTAGG13500.09.5937831
TTAGGAC8050.09.5561733
TTTCGTT800.0045058679.50277117
AGGACCT9500.09.4971855
TAGGACG12400.09.4205954
GACGTGA5950.09.4150347
Produced by FastQC (version 0.11.5)