##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512997_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2212294
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.780450518782764	31.0	30.0	34.0	22.0	34.0
2	28.921519020528013	31.0	30.0	34.0	16.0	34.0
3	29.083129999900557	31.0	30.0	34.0	22.0	34.0
4	32.50009311601442	35.0	33.0	37.0	24.0	37.0
5	32.595202988391236	35.0	33.0	37.0	25.0	37.0
6	32.641921010498606	37.0	33.0	37.0	25.0	37.0
7	32.47061059696406	36.0	33.0	37.0	23.0	37.0
8	32.467331647601995	37.0	33.0	37.0	23.0	37.0
9	33.751688066775934	38.0	34.0	39.0	22.0	39.0
10	33.639483721422195	38.0	34.0	39.0	19.0	39.0
11	33.69402755691603	38.0	34.0	39.0	22.0	39.0
12	33.5816537042545	38.0	34.0	39.0	19.0	39.0
13	33.57175854565442	38.0	34.0	39.0	19.0	39.0
14	34.55671850124803	39.0	34.0	41.0	18.0	41.0
15	34.48150110247553	39.0	34.0	41.0	17.0	41.0
16	34.361210580510544	39.0	34.0	41.0	17.0	41.0
17	34.30550008271957	39.0	34.0	41.0	17.0	41.0
18	33.99993084101842	39.0	33.0	41.0	11.0	41.0
19	34.225610158505155	39.0	34.0	41.0	18.0	41.0
20	34.30221254498724	39.0	34.0	41.0	18.0	41.0
21	34.237457589271585	39.0	34.0	41.0	16.0	41.0
22	34.15786192974352	39.0	34.0	41.0	10.0	41.0
23	33.88244103179776	39.0	34.0	41.0	8.0	41.0
24	33.88802302044846	39.0	34.0	41.0	2.0	41.0
25	33.80407305719764	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7413108090621776
1101	2	1.1002152027018717
1101	3	1.0543973736236119
1101	4	1.0594881559719482
1101	5	0.8942973146878046
1101	6	1.0606404244307512
1101	7	1.07943594928404
1101	8	0.9529606429651736
1101	9	0.7273737562636171
1101	10	0.9551171675844685
1101	11	1.1715762538187775
1101	12	1.2832098775798997
1101	13	1.1297654300670175
1101	14	1.0718212891314423
1101	15	0.8950255960608473
1101	16	1.4073517838438931
1101	17	1.4262882254900333
1101	18	1.6581947608442107
1101	19	1.0929929623301433
1101	20	1.3494424127986449
1101	21	1.3271350297337818
1101	22	1.226091990052403
1101	23	1.5080442604098394
1101	24	1.103270310194759
1101	25	1.4037795153852173
1102	1	0.5930587490864916
1102	2	0.76029354931811
1102	3	0.8076510095369791
1102	4	0.9313126415408632
1102	5	0.8494186618893877
1102	6	0.8778600200901678
1102	7	0.9375169074541532
1102	8	0.9071517366659307
1102	9	0.9532889257894794
1102	10	1.1690187390194708
1102	11	1.0425713458985655
1102	12	1.1563365379428134
1102	13	0.9831176746525472
1102	14	1.0972749340501693
1102	15	1.280715631632006
1102	16	1.298690430338553
1102	17	1.1246330406550555
1102	18	1.3313551913003323
1102	19	1.336607262838946
1102	20	1.2662027351904683
1102	21	1.0196214091295275
1102	22	1.3643071280595152
1102	23	1.5735024080076556
1102	24	1.5774226808552996
1102	25	1.4277648366513702
1103	1	0.13509686129280496
1103	2	0.45469703959476604
1103	3	0.48956145192500244
1103	4	0.492440585293096
1103	5	0.1292703618307982
1103	6	0.47313149212028804
1103	7	0.27296583799769536
1103	8	0.2623590373768039
1103	9	0.5844866375017546
1103	10	0.5639094122896822
1103	11	0.5009714406176755
1103	12	0.7004364508693968
1103	13	0.7081951254329084
1103	14	0.7766719123884229
1103	15	0.887944167247575
1103	16	0.8771403487044935
1103	17	0.720672051993823
1103	18	1.139468004898994
1103	19	1.1528123559131345
1103	20	0.9720454796328184
1103	21	0.8571957961750272
1103	22	0.9212381591826926
1103	23	1.2081050268510793
1103	24	0.9920396561090641
1103	25	1.036737112526879
1104	1	0.3849285546869474
1104	2	0.4023951058882105
1104	3	0.42019573169230284
1104	4	0.5593276864434316
1104	5	0.39085865660315733
1104	6	0.37297955594483767
1104	7	0.5796214201424164
1104	8	0.5910245470735518
1104	9	0.5209558616947518
1104	10	0.5200624660932576
1104	11	0.4525413760770576
1104	12	0.5643207522718185
1104	13	0.6703810656504743
1104	14	0.6470469217005501
1104	15	0.8383660219700246
1104	16	0.7116945680345381
1104	17	0.6003971319921462
1104	18	0.9515908634737826
1104	19	0.8289876074353799
1104	20	0.8222156099545188
1104	21	0.9545760283684643
1104	22	0.8469682777982044
1104	23	1.1003945627308838
1104	24	0.9974535114288656
1104	25	0.7210494056276033
1105	1	0.8118438609159853
1105	2	0.9869126561917803
1105	3	0.9290039620037831
1105	4	1.0973801382035546
1105	5	0.8060873485533122
1105	6	0.558181436877419
1105	7	0.9553281074403515
1105	8	0.9048360003122298
1105	9	0.7923493467108997
1105	10	0.9726137590433268
1105	11	0.8890275446662983
1105	12	1.0799513122458606
1105	13	0.9312475957001283
1105	14	1.1149360360197775
1105	15	1.3748193243451396
1105	16	1.2589601754522803
1105	17	1.1221680689170412
1105	18	1.261038583688034
1105	19	1.1694493989752601
1105	20	1.2004205541541424
1105	21	1.2181316431832272
1105	22	1.134939243427958
1105	23	1.2657027025965775
1105	24	1.3475967384834533
1105	25	1.3409818727762541
1106	1	0.6740703153385468
1106	2	0.7345840278807287
1106	3	0.8051082587795797
1106	4	0.736741665410527
1106	5	0.8076892624152947
1106	6	0.7561302608170948
1106	7	0.5683233240405414
1106	8	0.6724238539158449
1106	9	0.7460338364543944
1106	10	0.8987392051990142
1106	11	0.765561857913923
1106	12	0.9817930864340809
1106	13	0.9448645329435834
1106	14	1.058242142228572
1106	15	1.1113998022332652
1106	16	1.0627919159228227
1106	17	0.9733677261757592
1106	18	1.1681055726229985
1106	19	0.7949766795833924
1106	20	0.8816811157695454
1106	21	0.7846626553703757
1106	22	0.8660586209040133
1106	23	1.1479673890732371
1106	24	1.0773720948327181
1106	25	1.1831532760532966
1107	1	0.6650953826974977
1107	2	0.7069806760345081
1107	3	0.8204245802478312
1107	4	0.8139518514804251
1107	5	0.800642511779273
1107	6	0.7474358180918159
1107	7	0.8597212361364939
1107	8	0.8333631757557711
1107	9	0.9513013212359525
1107	10	0.7286943863855697
1107	11	0.7606574214074087
1107	12	0.9847072856444754
1107	13	0.9717930541841966
1107	14	1.2828204889366148
1107	15	1.3447378738328197
1107	16	1.1354814154063604
1107	17	1.188993231926382
1107	18	1.5362969074538242
1107	19	1.4747523472238555
1107	20	1.3058599753356077
1107	21	1.273522324031525
1107	22	1.250154892324261
1107	23	1.3207576167052792
1107	24	1.3948369525076671
1107	25	1.3406016981775508
1108	1	0.37905933191902363
1108	2	0.4379927501259431
1108	3	0.400473528559516
1108	4	0.4301854366464255
1108	5	0.4256960224034998
1108	6	0.5844205287809814
1108	7	0.5996798938609373
1108	8	0.5923362692393184
1108	9	0.6706618250933047
1108	10	0.604858365193401
1108	11	0.5194312000887749
1108	12	0.46468874838602403
1108	13	0.5263153074036708
1108	14	0.6714463884714803
1108	15	0.6648403916795189
1108	16	0.5828747443497448
1108	17	0.6984595395427604
1108	18	0.8863565382534233
1108	19	0.8862765392625249
1108	20	0.8077876669363704
1108	21	0.6497956150625583
1108	22	0.6821639986215615
1108	23	0.7649579814284593
1108	24	0.9522648162588183
1108	25	0.9673125653834873
1109	1	0.3160021154556141
1109	2	0.22880425597177734
1109	3	0.38814944290759357
1109	4	0.5140850158879573
1109	5	0.45204506243555187
1109	6	0.3438890695942902
1109	7	0.34392372526117754
1109	8	0.4822650150355088
1109	9	0.587980688147141
1109	10	0.7474851254843458
1109	11	0.2352995709463528
1109	12	0.5610604360687574
1109	13	0.5906642399020043
1109	14	0.5074251715438365
1109	15	0.5922997173775215
1109	16	0.6153940233754938
1109	17	0.5519023889765364
1109	18	-1.0463662024401543
1109	19	0.37881884624113127
1109	20	0.5007447983563509
1109	21	0.5211526643897173
1109	22	0.6870807157219829
1109	23	0.2257134189300558
1109	24	0.6182083623668504
1109	25	0.6270089924444804
1110	1	0.288594255542705
1110	2	0.30699995080048836
1110	3	0.46048299116908353
1110	4	0.3541481188800901
1110	5	0.4198129426121895
1110	6	0.3776892393627733
1110	7	0.4782694272416066
1110	8	0.41016054768333277
1110	9	0.6894201040763832
1110	10	0.7970255268650916
1110	11	0.6431756276809253
1110	12	0.11153734087086775
1110	13	0.4943529107128981
1110	14	0.5603959747864238
1110	15	0.6590946413707641
1110	16	0.7548613545651435
1110	17	0.3701906977136673
1110	18	0.9036550912680497
1110	19	0.8039318288164523
1110	20	0.8096436957006574
1110	21	0.7268187280306222
1110	22	0.8318044770787552
1110	23	0.7169755724068452
1110	24	0.9115368611620411
1110	25	0.8760819331620908
1111	1	0.3028087043219365
1111	2	0.5107059601469004
1111	3	0.5082219873188016
1111	4	0.4183254504002747
1111	5	0.3565837685273223
1111	6	0.5115644184694972
1111	7	0.48393721735735085
1111	8	0.3211957986636662
1111	9	0.6323767630353885
1111	10	0.5290241406803986
1111	11	0.37620903547485796
1111	12	0.4857567440007955
1111	13	0.6761376945583564
1111	14	0.5215428359194263
1111	15	0.8218321429795594
1111	16	0.6366774825915087
1111	17	0.6439058164121931
1111	18	0.9099180332577319
1111	19	0.5550132515031478
1111	20	0.5372215850212996
1111	21	0.6775491798632061
1111	22	0.45581092225623365
1111	23	0.7581104093888413
1111	24	0.9033788154370015
1111	25	0.405689992023909
1112	1	-0.27545409176890345
1112	2	-0.02194051034559763
1112	3	-0.04660009404239318
1112	4	0.07160851831665127
1112	5	0.07511428982334678
1112	6	0.049824978892928584
1112	7	-0.29370057580985787
1112	8	0.03619506231288483
1112	9	0.228436826676095
1112	10	0.2782810554819548
1112	11	0.228032515821873
1112	12	0.0793224899628413
1112	13	-0.13559088122310925
1112	14	0.0364026042455734
1112	15	0.03693980262451646
1112	16	0.24460666580993262
1112	17	0.343029502534165
1112	18	0.27948200229970865
1112	19	0.17179154830203203
1112	20	0.06327388512142562
1112	21	0.11913914265208803
1112	22	0.0648166454680279
1112	23	0.1870982892002644
1112	24	-0.09332123622853317
1112	25	0.274598020118475
1113	1	-0.278847679590033
1113	2	-0.1018590967782842
1113	3	-0.16852262130475282
1113	4	0.12086130165472042
1113	5	-0.18356879409880378
1113	6	0.0795183247730833
1113	7	-0.15216542005791212
1113	8	0.060795054021347994
1113	9	-0.027380903302763215
1113	10	-0.39421464425780073
1113	11	-0.1897537829505893
1113	12	-0.016649384496936648
1113	13	-0.12633955281620501
1113	14	-0.0031248247268038654
1113	15	-0.2947335011877925
1113	16	-0.3079747027796671
1113	17	-0.3278362831951469
1113	18	-0.4073231800712165
1113	19	-0.3406205789403032
1113	20	-0.6244399252259569
1113	21	-0.4103963510193509
1113	22	-0.24112494921474337
1113	23	-0.5476321691578079
1113	24	-0.15962432654924896
1113	25	-0.15784467632265375
1114	1	-1.4946437492000726
1114	2	-1.1967319527068199
1114	3	-1.0167389259742272
1114	4	-1.1109145182768785
1114	5	-1.1906026571352442
1114	6	-1.6137075353018275
1114	7	-1.663039011652458
1114	8	-1.3304500489070534
1114	9	-1.4196061868701761
1114	10	-1.5196864902961096
1114	11	-2.132944097624648
1114	12	-1.7147928888844035
1114	13	-1.6464660307798233
1114	14	-1.9679722506620934
1114	15	-1.870924095273466
1114	16	-2.5883771026542277
1114	17	-4.5736569575320765
1114	18	-3.4743798440380864
1114	19	-2.5041296586386323
1114	20	-1.7094312776135112
1114	21	-2.7443132655606632
1114	22	-2.823237654661856
1114	23	-2.6219191095535344
1114	24	-2.228276348274978
1114	25	-2.12324243702135
1115	1	-1.2433261263294746
1115	2	-0.8996818813461545
1115	3	-0.9282727235747892
1115	4	-0.9029761093225019
1115	5	-0.7934156641572514
1115	6	-1.0003487122187664
1115	7	-1.9738402085204214
1115	8	-1.3479941883670143
1115	9	-1.100213436417917
1115	10	-0.843201871866647
1115	11	-1.6257193436654944
1115	12	-1.2586597622142648
1115	13	-1.1499944327117078
1115	14	-1.1191449773065187
1115	15	-2.5734192295537923
1115	16	-1.2170964723024795
1115	17	-1.3031343016683437
1115	18	-1.392565673689809
1115	19	-1.3769155568236613
1115	20	-1.23070052602354
1115	21	-1.7875419285280785
1115	22	-1.8991109830750972
1115	23	-1.292264487446083
1115	24	-1.339411222109419
1115	25	-1.5803885806696698
1116	1	-1.431056435001178
1116	2	-2.161143368055992
1116	3	-1.979597124222476
1116	4	-1.5445836850256853
1116	5	-2.0715475706977173
1116	6	-1.877487607725552
1116	7	-1.4916863089053578
1116	8	-2.184608937042565
1116	9	-2.159068952970614
1116	10	-3.0512760387380666
1116	11	-1.7952478172809094
1116	12	-1.7784289900306973
1116	13	-2.2137477116793356
1116	14	-2.1208735307718527
1116	15	-1.7705524183864298
1116	16	-1.7351143013077035
1116	17	-1.972990069830665
1116	18	-1.802565656594652
1116	19	-2.1583427102422803
1116	20	-2.4818013375617127
1116	21	-2.9977186442718313
1116	22	-2.2337837996467087
1116	23	-2.117589516884717
1116	24	-2.7748524504368355
1116	25	-2.416274764845742
1201	1	0.07288063798064925
1201	2	0.12398116534048498
1201	3	0.30360712698785264
1201	4	0.29711479516435446
1201	5	0.28000066492142395
1201	6	0.3644373859938881
1201	7	0.27931412260689115
1201	8	-0.1701979034292762
1201	9	0.16437318689560954
1201	10	0.3363292887965912
1201	11	-0.11818208109612982
1201	12	-0.5385122346850082
1201	13	-0.3124373914677392
1201	14	0.30339590797120053
1201	15	-0.029969749581681526
1201	16	0.4720368691135306
1201	17	0.5524957827205554
1201	18	0.7854369410717794
1201	19	0.33631761957964557
1201	20	0.3422966636278417
1201	21	0.2407576562374132
1201	22	0.35077472554933564
1201	23	0.6304381365406613
1201	24	0.45483471656083196
1201	25	0.34076665489845226
1202	1	0.6696920992299162
1202	2	0.8767607105820829
1202	3	0.5321694919912119
1202	4	0.7631420311864048
1202	5	0.7388137906827197
1202	6	0.6972173109255024
1202	7	0.7833654762936462
1202	8	0.7343627247984728
1202	9	1.1568265416712222
1202	10	1.3316358303043643
1202	11	0.844866119326305
1202	12	0.9892403495035964
1202	13	1.0254524261646978
1202	14	1.1973668592355367
1202	15	0.700487697272429
1202	16	1.0630850033964094
1202	17	1.1751990408895523
1202	18	1.444399370744918
1202	19	1.2161108232508795
1202	20	1.1796111284088369
1202	21	1.2317883982680584
1202	22	1.3727275447783072
1202	23	1.3744254083515628
1202	24	1.4093784730791015
1202	25	1.2294421552778374
1203	1	0.2536525794970572
1203	2	0.5806015188625828
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2211	19	-0.18031291878061495
2211	20	-0.31423407331686803
2211	21	-0.24368959279815527
2211	22	-0.3568369509253202
2211	23	0.18590721046791003
2211	24	-0.1157403539378592
2211	25	-0.24142584835673375
2212	1	-0.1105928671988643
2212	2	-0.2135836003759266
2212	3	-0.24572326853465043
2212	4	-0.37886560103384426
2212	5	-0.33465015372674856
2212	6	-0.3446896791125198
2212	7	-0.501797070569669
2212	8	-0.43333269796766416
2212	9	-0.2545156709236167
2212	10	-0.17606740541828714
2212	11	-0.45441500674927937
2212	12	-0.20275858447006812
2212	13	-0.40162052984558727
2212	14	-0.32150064494283725
2212	15	-0.5603396551978221
2212	16	-0.29229483912561705
2212	17	-0.3234961385263091
2212	18	-3.058052983052434
2212	19	-1.3189487114610614
2212	20	-0.5969250233180077
2212	21	-0.7374712208599234
2212	22	-0.3953139185635379
2212	23	-0.4931450622841851
2212	24	-0.9060628331509264
2212	25	-0.44899240443999133
2213	1	-0.029658127956913916
2213	2	0.005567154824984044
2213	3	-0.3118389710139873
2213	4	-0.3366883425556466
2213	5	-0.14855724976550277
2213	6	-0.19222458934335407
2213	7	-0.1093775778366961
2213	8	-0.3729166250124081
2213	9	-0.17586078349130219
2213	10	-0.2706812555180278
2213	11	-0.2252898998812256
2213	12	-0.4190595249089384
2213	13	-0.1787650154540188
2213	14	-0.2941424232272496
2213	15	-0.49935361929016153
2213	16	-0.9819500115394106
2213	17	-0.49847476491559206
2213	18	-0.3914813102111694
2213	19	-0.30669450798428954
2213	20	-1.044630922434564
2213	21	-0.6488419817604694
2213	22	-0.7002440981018694
2213	23	-1.0394545521673493
2213	24	-0.6558490970437703
2213	25	-0.8086390274094981
2214	1	-1.6796942914478734
2214	2	-3.640690887907187
2214	3	-1.8568627523889916
2214	4	-1.4831011029539418
2214	5	-1.389312046248289
2214	6	-1.4334759872104144
2214	7	-1.5639963939502834
2214	8	-1.3216538184809679
2214	9	-1.4675407351855299
2214	10	-1.4917535194842344
2214	11	-1.0852464284494445
2214	12	-1.769118355243382
2214	13	-1.7253500814841338
2214	14	-2.720707963793849
2214	15	-2.973192225721334
2214	16	-2.36139109044354
2214	17	-2.7685572590584258
2214	18	-2.156164675099383
2214	19	-4.434846821648257
2214	20	-2.4490544368863247
2214	21	-2.4342481085800465
2214	22	-2.480976716818283
2214	23	-2.5828888328994957
2214	24	-2.824947511587421
2214	25	-2.496991561722492
2215	1	-0.7702990996868699
2215	2	-2.5002623527192753
2215	3	-1.188264506970004
2215	4	-0.7902471198408492
2215	5	-0.8459871323279131
2215	6	-1.3121274334947586
2215	7	-1.7896125338113045
2215	8	-1.7013597960211584
2215	9	-1.274423650841861
2215	10	-2.6251980470437317
2215	11	-1.7812505096212305
2215	12	-1.8880138143582847
2215	13	-1.2577548406178423
2215	14	-2.6201914036044194
2215	15	-2.3828900077645088
2215	16	-2.1355303788299977
2215	17	-2.3584782123918266
2215	18	-1.1586004986643843
2215	19	-1.8432850331292272
2215	20	-1.9168353196896746
2215	21	-1.8344723369618094
2215	22	-1.452817030731076
2215	23	-1.7725419897106889
2215	24	-1.6267175931370872
2215	25	-1.5682613152526628
2216	1	-2.1897978255970756
2216	2	-2.346363185739797
2216	3	-2.7876755913884956
2216	4	-3.438760701218353
2216	5	-2.5601003393839186
2216	6	-2.618591729820153
2216	7	-2.383767064412197
2216	8	-3.1071247509217734
2216	9	-3.34279038879237
2216	10	-2.8499989488728623
2216	11	-3.346366339113718
2216	12	-3.866727366216587
2216	13	-4.3011433112253386
2216	14	-3.700259192640413
2216	15	-3.5828007493080456
2216	16	-2.867435955097296
2216	17	-2.8505045654129404
2216	18	-2.9762758757204537
2216	19	-3.2384561990267215
2216	20	-3.060510563750814
2216	21	-3.416632877198243
2216	22	-3.95133168424465
2216	23	-3.977462902362973
2216	24	-3.907342742745957
2216	25	-3.9549892218865494
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117789.0
3	3.0
4	1025.0
5	5753.0
6	7480.0
7	6448.0
8	5871.0
9	5201.0
10	4910.0
11	4716.0
12	4574.0
13	4307.0
14	4453.0
15	4457.0
16	4496.0
17	4884.0
18	5407.0
19	6434.0
20	8428.0
21	10553.0
22	13141.0
23	16006.0
24	18304.0
25	21016.0
26	23111.0
27	25715.0
28	29048.0
29	34734.0
30	42377.0
31	53156.0
32	67011.0
33	84630.0
34	109294.0
35	142378.0
36	194137.0
37	298341.0
38	822706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.310590028511356	17.85277980026175	12.329878855100175	25.506751316126717
2	15.21457389552167	19.519029974595348	40.22223870243637	25.044157427446613
3	19.274556059436556	23.743783068603634	28.935731885267064	28.045928986692747
4	12.565668039187846	16.061165669305296	36.98300973727358	34.39015655423327
5	13.65404895025206	37.45638485874031	35.3371728525252	13.552393338482439
6	33.53861761785837	35.75283060836605	17.46111473797093	13.24743703580464
7	28.845724350761202	31.1021575135924	21.557142327539516	18.494975808106883
8	25.95411529545914	36.102318469310205	19.96784462697371	17.97572160825695
9	26.62516247894838	14.998112418477955	19.419981237213612	38.95674386536005
10	15.05941680923325	28.731974218656752	34.08423424563891	22.12437472647109
11	34.88103456772374	22.35701068014672	23.510654602617436	19.251300149512108
12	23.552809925500874	24.50224654789365	30.935809946298054	21.009133580307417
13	29.064432502619997	21.443878699757757	25.289691721892332	24.201997075729913
14	21.449644547352314	21.530080883958718	26.948614338285658	30.071660230403307
15	24.01611633567229	29.615688423538966	22.85115901158808	23.517036229200663
16	23.005502280937332	27.68804734583893	26.651159005520363	22.655291367703374
17	22.649852426285484	28.018524479874994	27.03139920136578	22.300223892473742
18	22.714088422790244	27.133236919935854	28.257753193769208	21.894921463504694
19	23.75869112887693	27.08173391242543	27.02761357810012	22.13196138059752
20	23.952590435800865	26.970417625543114	27.119301561178954	21.957690377477064
21	23.381413690920255	26.664478318625285	26.86359990487016	23.0905080855843
22	23.507409883974585	27.175391743446532	26.334735331576425	22.982463041002458
23	22.8317517385185	27.124776821742678	26.846541522571915	23.1969299171669
24	23.300344506433497	26.919470490899062	27.109717613230494	22.670467389436944
25	23.23248444360249	26.56806181054177	26.818221070616527	23.381232675239204
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	198.0
1	198.0
2	396.5
3	595.0
4	595.0
5	595.0
6	1126.5
7	1658.0
8	1658.0
9	1658.0
10	2372.5
11	3087.0
12	3087.0
13	3087.0
14	5345.0
15	7603.0
16	7603.0
17	7603.0
18	14035.0
19	20467.0
20	20467.0
21	20467.0
22	32655.0
23	44843.0
24	44843.0
25	44843.0
26	64824.0
27	84805.0
28	84805.0
29	84805.0
30	110623.5
31	136442.0
32	136442.0
33	136442.0
34	159956.5
35	183471.0
36	183471.0
37	183471.0
38	205748.5
39	228026.0
40	228026.0
41	228026.0
42	246817.0
43	265608.0
44	265608.0
45	265608.0
46	287970.5
47	310333.0
48	310333.0
49	310333.0
50	301367.5
51	292402.0
52	292402.0
53	292402.0
54	265104.0
55	237806.0
56	237806.0
57	237806.0
58	205747.0
59	173688.0
60	173688.0
61	173688.0
62	142672.0
63	111656.0
64	111656.0
65	111656.0
66	87147.5
67	62639.0
68	62639.0
69	62639.0
70	45864.5
71	29090.0
72	29090.0
73	29090.0
74	20371.0
75	11652.0
76	11652.0
77	11652.0
78	7966.0
79	4280.0
80	4280.0
81	4280.0
82	2820.0
83	1360.0
84	1360.0
85	1360.0
86	901.5
87	443.0
88	443.0
89	443.0
90	276.0
91	109.0
92	109.0
93	109.0
94	63.5
95	18.0
96	18.0
97	18.0
98	16.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35750221263539117
2	0.04013933048681595
3	0.035845145355906584
4	0.03579994340716017
5	0.04095296556425141
6	0.018171183396058572
7	0.017899971703580084
8	0.024092638681838852
9	0.021199713962068333
10	0.020883300320843432
11	0.019572443807197415
12	0.020612088628364944
13	0.020792896423350606
14	0.02743758288907351
15	0.028251217966508972
16	0.02187774319326455
17	0.023414609450642637
18	0.10694781073401637
19	0.02513228350300638
20	0.022781782168192835
21	0.026217130272920327
22	0.020386078884632876
23	0.022465368526967937
24	0.01961764575594383
25	0.022736580219446422
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2212294.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.50750051255959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8428774246604	56.19186344031375
2	12.600330408438559	17.516349446459266
3	3.481748535198645	7.260229142847703
4	1.328512189146023	3.6936624667203546
5	0.6032508998636862	2.0965231115738603
6	0.34174876430862344	1.425246144620895
7	0.19600627861977998	0.9536734558140479
8	0.1364627686828818	0.7588148771336566
9	0.0842740556311625	0.5271911068480654
>10	0.3523591965061601	4.1266820897582805
>50	0.01801073302933483	0.8589687579925691
>100	0.011289933149066475	1.6732654096584039
>500	0.001890324379334205	0.8699491271790313
>1k	0.0011081211878841673	1.4579044328076365
>5k	1.3036719857460792E-4	0.5896769902725897
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7085	0.3202558068683457	No Hit
CCCATGTACTCTGCGTTGATACCAC	5930	0.26804755606623715	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3171	0.14333537947487993	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2969	0.1342045858281042	No Hit
GTCCTACAGTGGACATTTCTAAATT	2575	0.11639501802201697	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2435	0.11006674519751895	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.5201948746414354E-5	0.0
3	0.0	0.0	0.0	9.040389749282871E-5	0.0
4	0.0	0.0	0.0	1.3560584623924305E-4	0.0
5	0.0	0.0	0.0	1.3560584623924305E-4	0.0
6	0.0	4.5201948746414354E-5	0.0	1.3560584623924305E-4	0.0
7	0.0	4.5201948746414354E-5	0.0	1.3560584623924305E-4	0.0
8	0.0	4.5201948746414354E-5	0.0	1.3560584623924305E-4	0.0
9	0.0	4.5201948746414354E-5	0.0	1.8080779498565741E-4	0.0
10	0.0	4.5201948746414354E-5	0.0	2.2600974373207178E-4	0.0
11	0.0	4.5201948746414354E-5	0.0	2.2600974373207178E-4	0.0
12	0.0	4.5201948746414354E-5	0.0	2.2600974373207178E-4	0.0
13	0.0	1.3560584623924305E-4	0.0	2.712116924784861E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	470	0.0	12.124065	4
GGACCGT	85	5.3546348E-5	11.17038	6
GTAGGAC	1165	0.0	10.597716	3
ACGAAAT	90	9.485871E-5	10.559113	12
TGTAGGA	1290	0.0	10.454266	2
TGGACTG	410	0.0	10.1921015	5
CCGTCCC	75	0.0026583348	10.128728	9
ACCGCAT	75	0.0026588333	10.128499	8
CGAAATC	95	1.6389534E-4	10.00337	13
TAGGACA	380	0.0	9.997037	4
TGGCGAG	1145	0.0	9.876243	18
AGGACGT	1150	0.0	9.827521	5
GTCTTAA	165	9.824362E-9	9.811824	1
GGCGAGG	520	0.0	9.677853	19
CTGTAGG	1350	0.0	9.593783	1
TTAGGAC	805	0.0	9.556173	3
TTTCGTT	80	0.004505867	9.502771	17
AGGACCT	950	0.0	9.497185	5
TAGGACG	1240	0.0	9.420595	4
GACGTGA	595	0.0	9.415034	7
>>END_MODULE