FastQCFastQC Report
Thu 26 May 2016
SRR1512997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212294
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC69490.3141083418388334No Hit
CCCATGTACTCTGCGTTGATACCAC68920.31153183076028773No Hit
GTATCAACGCAGAGTACTTTTTTTT40650.18374592165417436No Hit
TATCAACGCAGAGTACTTTTTTTTT30260.13678109690664983No Hit
GAGTACATGGGAAGCAGTGGTATCA25310.11440613227717474No Hit
GTCCTACAGTGGACATTTCTAAATT24060.10875588868387294No Hit
CTGTAGGACGTGGAATATGGCAAGA24010.10852987894014088No Hit
GGTATCAACGCAGAGTACTTTTTTT23930.10816826335016956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG602.5339608E-514.2709025
TAGGACC3950.012.76553254
TGGCGAG11800.011.01887618
GGCGAGG5700.010.82274119
AGGACGT12400.010.7415395
GACGTGA7350.010.7321987
GGACGTG12550.010.6093086
TTAGGAC8700.010.5993243
CGTGAAA7850.010.4094659
TAGGACG13250.010.3396734
GTAGGAC11200.010.27053453
ACGTGAA8200.010.1985238
TATGGCG13000.010.15005416
ATGGCGA13550.09.94823417
CCCCTAC2201.8189894E-129.93940451
TCTATAC2600.09.8722493
TGTAGGA12000.09.8234982
TTTAGGA10050.09.7430942
GGCGAGA7500.09.61725319
ATATGGC23100.09.61613915
Produced by FastQC (version 0.11.5)