##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512997_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2212294
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.261760869034585	31.0	31.0	34.0	26.0	34.0
2	30.406187875571693	31.0	31.0	34.0	27.0	34.0
3	30.531717755415873	31.0	31.0	34.0	27.0	34.0
4	34.00025313091298	37.0	35.0	37.0	30.0	37.0
5	33.62233410206781	37.0	35.0	37.0	28.0	37.0
6	33.476978195483966	37.0	35.0	37.0	28.0	37.0
7	33.34707638315703	37.0	35.0	37.0	27.0	37.0
8	33.23239587505096	37.0	35.0	37.0	27.0	37.0
9	34.66704063745596	39.0	35.0	39.0	27.0	39.0
10	34.56473325878025	39.0	35.0	39.0	27.0	39.0
11	34.594703958877076	39.0	35.0	39.0	27.0	39.0
12	34.43088170017186	39.0	35.0	39.0	26.0	39.0
13	34.40927562069056	39.0	35.0	39.0	26.0	39.0
14	35.462019514585315	40.0	36.0	41.0	26.0	41.0
15	35.46509324710007	40.0	36.0	41.0	26.0	41.0
16	35.42777813437093	40.0	36.0	41.0	26.0	41.0
17	35.410489745033885	40.0	36.0	41.0	26.0	41.0
18	35.332075664446045	40.0	36.0	41.0	25.0	41.0
19	35.297904799271706	40.0	35.0	41.0	25.0	41.0
20	35.2776366974733	40.0	36.0	41.0	25.0	41.0
21	35.22897227945291	40.0	36.0	41.0	25.0	41.0
22	35.18944001113776	40.0	36.0	41.0	25.0	41.0
23	35.081645115884236	40.0	35.0	41.0	24.0	41.0
24	35.05837741276702	40.0	35.0	41.0	24.0	41.0
25	34.96365311301301	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7364489539644694
1101	2	0.6820373102441053
1101	3	0.6727942953305401
1101	4	0.5550447970892947
1101	5	0.8986446780302018
1101	6	0.7846665306136629
1101	7	0.7950334549336091
1101	8	0.8069026678965443
1101	9	0.7542197981216248
1101	10	0.9590797508356772
1101	11	1.0388659488072065
1101	12	1.0594716156238846
1101	13	1.0671953495553979
1101	14	1.2193392315844065
1101	15	1.1957531490422326
1101	16	1.1900719902734096
1101	17	1.1742908450950864
1101	18	0.648131547104569
1101	19	1.0716565050693632
1101	20	0.93326876938238
1101	21	1.054679717521239
1101	22	1.110349308561183
1101	23	1.0716922954282424
1101	24	1.0195159587231402
1101	25	1.0698720663251962
1102	1	0.5045970020530817
1102	2	0.4496634978257177
1102	3	0.469995430005671
1102	4	0.40454139756657526
1102	5	0.6159763903990623
1102	6	0.6270272852626846
1102	7	0.6431846548975884
1102	8	0.5903958463377137
1102	9	0.8037974468226068
1102	10	0.8654510417537722
1102	11	0.8074476048925803
1102	12	0.8008480846229347
1102	13	0.8958085243944325
1102	14	0.9508971585509514
1102	15	0.8898694982700235
1102	16	0.8324588604611733
1102	17	0.8737969011984674
1102	18	0.9620178094306553
1102	19	1.0188688964422354
1102	20	0.9354620243690945
1102	21	0.9087037618276028
1102	22	0.9613358455506926
1102	23	0.9102226209719433
1102	24	0.9825584189869971
1102	25	1.007313193796044
1103	1	0.4786317344301061
1103	2	0.4237796676612646
1103	3	0.3912877865497002
1103	4	0.4744267079354785
1103	5	0.4900406132720647
1103	6	0.5498303416139976
1103	7	0.5427407246314644
1103	8	0.5627071104401793
1103	9	0.6012272736413067
1103	10	0.752823123996869
1103	11	0.6879736978649689
1103	12	0.6308642541526552
1103	13	0.7358702490947522
1103	14	0.8335836160346162
1103	15	0.6603714876770397
1103	16	0.6893858284493533
1103	17	0.8477969440142985
1103	18	0.8463807251538071
1103	19	0.8786605056752492
1103	20	0.8320424812140175
1103	21	0.55735486654158
1103	22	0.738311850772476
1103	23	0.7077907691391871
1103	24	0.7478647509678424
1103	25	0.7449650425443082
1104	1	0.40609627563051376
1104	2	0.44698455921124847
1104	3	0.39421777530287727
1104	4	0.4579192407653778
1104	5	0.5288778464611497
1104	6	0.47673649887438785
1104	7	0.5449114626290523
1104	8	0.37084755858221996
1104	9	0.5558707724642318
1104	10	0.610733207579834
1104	11	0.5992643742553696
1104	12	0.5037681207571296
1104	13	0.5755647663683519
1104	14	0.8202561243269315
1104	15	0.8073918886915052
1104	16	0.788400092832056
1104	17	0.7179100092350268
1104	18	0.822146122347938
1104	19	0.8035628121424381
1104	20	0.7851248964813351
1104	21	0.6969596260270094
1104	22	0.8078612329543233
1104	23	0.726871826254353
1104	24	0.9222223083516639
1104	25	0.920606857182868
1105	1	0.5441016785983237
1105	2	0.6766069544363909
1105	3	0.5853737913062602
1105	4	0.4834834653392832
1105	5	0.4773164465132922
1105	6	0.5320769189674479
1105	7	0.6828493497306312
1105	8	0.8069290566021792
1105	9	0.9219776377662612
1105	10	0.6832562033140164
1105	11	0.7287630328552339
1105	12	0.7839379040524364
1105	13	0.8692321178185836
1105	14	1.0140267453285219
1105	15	0.9516835708217144
1105	16	1.097324926016917
1105	17	0.793857630510928
1105	18	1.1909604163485383
1105	19	1.0735705658513481
1105	20	1.136507103375429
1105	21	0.91184029279502
1105	22	1.0166465333489754
1105	23	1.0721831253530354
1105	24	1.0209114814394127
1105	25	1.0103475028383642
1106	1	0.5225334949639695
1106	2	0.4382672079528156
1106	3	0.4380040754610448
1106	4	0.31913985548530377
1106	5	0.47562223684114713
1106	6	0.41799436065812756
1106	7	0.5659976660851882
1106	8	0.5592179801426695
1106	9	0.4945354073706483
1106	10	0.5739089304962235
1106	11	0.414743985443387
1106	12	0.5949293058149223
1106	13	0.5235045645068723
1106	14	0.7816339283357223
1106	15	0.7010353454659679
1106	16	0.7945236268466971
1106	17	0.6483693936244848
1106	18	0.7955489322561746
1106	19	0.8009044762775588
1106	20	0.8555486159891217
1106	21	0.796523581354144
1106	22	0.7604736130066172
1106	23	0.7835694410557537
1106	24	0.7509828035915405
1106	25	0.8376069825092358
1107	1	0.6318721874474029
1107	2	0.6104327456544674
1107	3	0.60510929376494
1107	4	0.6454595067577316
1107	5	0.5841834178973002
1107	6	0.677822429441207
1107	7	0.7496669309103652
1107	8	0.8433445841892038
1107	9	0.646706316578296
1107	10	0.8777799561030477
1107	11	0.8066793294564079
1107	12	0.6885833993625283
1107	13	0.8072604088741926
1107	14	0.9667312922600502
1107	15	0.9206673890311023
1107	16	0.8066234984667133
1107	17	0.7923477633012581
1107	18	0.8679885557675533
1107	19	1.0669537948442667
1107	20	1.0092688389645375
1107	21	1.0402689044537112
1107	22	0.9870243250869493
1107	23	1.1124166457048332
1107	24	1.0938530147019847
1107	25	1.0283196883549053
1108	1	0.4474575576754667
1108	2	0.3988556648275683
1108	3	0.3172817007415496
1108	4	0.35897278852013415
1108	5	0.4492227506607307
1108	6	0.39696430885214795
1108	7	0.4577735122916806
1108	8	0.4986513980717291
1108	9	0.4887272443052453
1108	10	0.5776105548852968
1108	11	0.5637482956507824
1108	12	0.7309487587771955
1108	13	0.5134059158799928
1108	14	0.6021259355683739
1108	15	0.5783771081314129
1108	16	0.44218767390562874
1108	17	0.6205511678206292
1108	18	0.5259329361374299
1108	19	0.5017542902184644
1108	20	0.563547267015494
1108	21	0.74434075203974
1108	22	0.6537994312245345
1108	23	0.5251399993776573
1108	24	0.6488321537405852
1108	25	0.6729410687784636
1109	1	0.14466161536861932
1109	2	0.14674761048632234
1109	3	0.1416029105221348
1109	4	0.3211348523282851
1109	5	0.22804785296529673
1109	6	-0.01163629058277138
1109	7	0.25850549563968883
1109	8	0.3472393295098044
1109	9	0.46586895211120805
1109	10	0.3489555066688794
1109	11	0.40171973102269476
1109	12	0.48698638377694436
1109	13	0.3539712744164305
1109	14	0.5158281576478032
1109	15	0.5421275892464621
1109	16	0.44768451575827584
1109	17	0.4244396592864561
1109	18	0.4098179917697422
1109	19	0.648733543422999
1109	20	0.6107345717417942
1109	21	0.5077154224718328
1109	22	0.683470050192561
1109	23	0.57454764594317
1109	24	0.2557372283486643
1109	25	0.40329583825960214
1110	1	0.2216360296624913
1110	2	0.2508200846473194
1110	3	0.261195091614546
1110	4	0.324295813712574
1110	5	0.25720796389276046
1110	6	0.2789416229179551
1110	7	0.18042861937986743
1110	8	0.30513655080196145
1110	9	0.3321023292158358
1110	10	0.4314769400211844
1110	11	0.29428272181522175
1110	12	0.520421541610304
1110	13	0.16464809146069825
1110	14	0.3974259380652754
1110	15	0.4743727532822746
1110	16	0.5208478567427122
1110	17	0.2801627590691922
1110	18	0.11131362050562643
1110	19	0.31749013615846877
1110	20	0.4141897705223556
1110	21	0.3602782066233772
1110	22	0.29721606710677406
1110	23	0.46536694194960404
1110	24	0.33797630616158614
1110	25	0.22450956994079974
1111	1	-0.29870151535338607
1111	2	-0.03571231717765144
1111	3	-0.11071499951611585
1111	4	-0.03453320902883661
1111	5	0.19321052633203095
1111	6	0.016689172912407457
1111	7	0.3101684982049733
1111	8	0.37875928410174
1111	9	-0.033365894873902846
1111	10	0.10073269093555126
1111	11	0.2895598594407218
1111	12	0.0874866233369076
1111	13	0.32371246200866466
1111	14	0.3697277587891534
1111	15	0.5280430018411479
1111	16	0.2877505010062791
1111	17	0.5756815424046806
1111	18	0.5593115089316427
1111	19	0.4813440622633962
1111	20	0.38724241437414264
1111	21	0.29424517363544567
1111	22	0.13670640846444826
1111	23	0.26269185094894
1111	24	0.425519828307678
1111	25	0.5161525589128502
1112	1	0.09459916046015948
1112	2	-0.12404352049740908
1112	3	-0.009764211782091081
1112	4	-0.1275777831721001
1112	5	0.015032108136082911
1112	6	-0.043299784245526496
1112	7	-0.08155027354580113
1112	8	0.09307510454570433
1112	9	-0.2531771664766822
1112	10	-0.06713415490710872
1112	11	-0.09232713741111809
1112	12	0.08915651849442696
1112	13	-0.04438251546609706
1112	14	-0.028979570570626834
1112	15	-0.12625555266921396
1112	16	-0.26909335923966893
1112	17	-0.04370011328285983
1112	18	-0.09114342357813854
1112	19	-0.12295353249075447
1112	20	0.03676831900082789
1112	21	0.18291813081854258
1112	22	-0.025292697591197566
1112	23	0.2168327634851437
1112	24	0.1686926952414325
1112	25	0.18051177808100505
1113	1	-0.2521652212605865
1113	2	-0.6414786020165764
1113	3	-0.22740603432585615
1113	4	-0.21432124830185728
1113	5	-0.05790914471608488
1113	6	-0.2727735606758017
1113	7	-0.16176000265483026
1113	8	-0.019190903803725234
1113	9	-0.11242024245146354
1113	10	-0.43861265438810904
1113	11	-0.10069408669424718
1113	12	-0.26322522728217024
1113	13	-0.16807661697179554
1113	14	-0.04399156593049014
1113	15	-0.002358934132608681
1113	16	-0.36579082194923274
1113	17	-0.05307190373209636
1113	18	-0.025445224798559707
1113	19	0.019839591160405234
1113	20	0.048893214672098395
1113	21	-0.1951415072790681
1113	22	-0.310282110229231
1113	23	-0.14553098338499382
1113	24	-0.45465292678466085
1113	25	-0.5515441882709737
1114	1	-0.6793096464095179
1114	2	-0.5897035130351789
1114	3	-0.6003059377696935
1114	4	-0.3554762203548023
1114	5	-0.615235800465662
1114	6	-1.0487234127762761
1114	7	-0.773303592634214
1114	8	-1.087315934014363
1114	9	-0.9163206870342435
1114	10	-0.9778909707504297
1114	11	-0.8722811092629286
1114	12	-0.9302276594600087
1114	13	-0.75312868538402
1114	14	-1.886153480831382
1114	15	-1.2037107204216326
1114	16	-1.2228056449119151
1114	17	-1.2285978047166424
1114	18	-1.2455824312247898
1114	19	-1.3408581597529903
1114	20	-0.6798153260094111
1114	21	-0.7882102130609994
1114	22	-0.8598097631363473
1114	23	-0.8222107246286186
1114	24	-1.4471991210688628
1114	25	-1.3508884552045544
1115	1	-1.5007151398034004
1115	2	-1.3245893335523498
1115	3	-0.9666911249095982
1115	4	-2.2528625785539305
1115	5	-1.4401660709692266
1115	6	-1.3917828690432827
1115	7	-1.1539145153351171
1115	8	-1.1736004767575423
1115	9	-1.6979986123986706
1115	10	-1.483115789473068
1115	11	-1.3233810494775256
1115	12	-1.8162688074750335
1115	13	-1.3300773777173234
1115	14	-2.1943428724750547
1115	15	-2.0755275828045328
1115	16	-1.5612316117849048
1115	17	-1.557939000530986
1115	18	-1.3928633070978265
1115	19	-1.2808649357539608
1115	20	-1.4805619910521557
1115	21	-1.5338165660693832
1115	22	-1.8608570950980479
1115	23	-2.0931333192086896
1115	24	-1.4497178891899551
1115	25	-1.4423463441951014
1116	1	-1.0862993224940567
1116	2	-1.0657974109828423
1116	3	-0.9004780859983796
1116	4	-1.0976670729017286
1116	5	-1.1253073181479394
1116	6	-1.6601861549810693
1116	7	-1.1380925592171423
1116	8	-1.355552544735346
1116	9	-1.4026409265482869
1116	10	-1.360961103480797
1116	11	-1.3031037069787033
1116	12	-1.8568042992739393
1116	13	-1.432332601880681
1116	14	-1.9453818306278237
1116	15	-1.6852727390861304
1116	16	-1.9863452673805924
1116	17	-1.995640130878293
1116	18	-2.6974341945742424
1116	19	-2.096942340137254
1116	20	-1.7843378760334545
1116	21	-1.8476944454182984
1116	22	-3.110622464071362
1116	23	-2.985701759280296
1116	24	-2.2756990618275736
1116	25	-2.6385542188148676
1201	1	0.6607067421053472
1201	2	0.6138976072192257
1201	3	0.5398517472731683
1201	4	0.5683071853621229
1201	5	0.57604275663914
1201	6	0.6704259138887068
1201	7	0.7556819018242251
1201	8	0.7413223192264269
1201	9	0.9114563750450557
1201	10	0.7731006638127482
1201	11	0.8456552555338845
1201	12	0.6323537022220407
1201	13	0.6485622846384089
1201	14	0.6760172856111026
1201	15	0.8622845230266378
1201	16	0.8830196897555567
1201	17	0.7556366322237551
1201	18	0.8281358851464873
1201	19	0.6458545547580243
1201	20	0.9160495446266168
1201	21	0.7998398183807112
1201	22	0.9044220972895047
1201	23	0.8643403988905476
1201	24	0.859006690677333
1201	25	0.7362371487028057
1202	1	0.681892832467895
1202	2	0.8080827158993671
1202	3	0.7214366591269084
1202	4	0.7349243494329087
1202	5	0.8364586641809453
1202	6	0.9618594335355226
1202	7	0.8418513256323834
1202	8	0.8031052332982895
1202	9	1.0874794406009798
1202	10	1.0336488504561103
1202	11	1.0442508924057066
1202	12	0.9738781331310165
1202	13	1.0751556507189903
1202	14	1.11237113465738
1202	15	1.2179634302052875
1202	16	1.059227701092368
1202	17	1.121973805529585
1202	18	1.066737376593359
1202	19	1.286776197150047
1202	20	1.3142623069878283
1202	21	1.2711980246760959
1202	22	1.1121278448777119
1202	23	1.2212888180021793
1202	24	1.1152423336486876
1202	25	1.1260281415983115
1203	1	0.5284730444666259
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2211	10	-0.6116730735756803
2211	11	-0.3541791361890958
2211	12	-0.3364929076577141
2211	13	-0.33218908714611217
2211	14	-1.314513582268404
2211	15	-0.5911626993370263
2211	16	-0.4135464238328055
2211	17	-0.22030106705780383
2211	18	-0.45231191187546926
2211	19	-0.30203581886686237
2211	20	-0.5872317057522451
2211	21	-0.526844936686345
2211	22	-0.41040457170488764
2211	23	-0.4605767363529836
2211	24	-0.6053663134762104
2211	25	-0.6025996895611954
2212	1	-0.34357384455362805
2212	2	-0.3651337736354421
2212	3	-0.21901533704335563
2212	4	-0.10964504807077446
2212	5	-0.22677946940021343
2212	6	-0.3388282922228498
2212	7	-0.2520673335151429
2212	8	-0.577936317569403
2212	9	-0.5549271573262686
2212	10	-0.6014028177973643
2212	11	-0.49529478074536826
2212	12	-0.29104705066281156
2212	13	-0.43385813196830014
2212	14	-0.40132097550213075
2212	15	-0.3848785598966842
2212	16	-0.34034408268184535
2212	17	-0.612866939648903
2212	18	-0.37002177292962557
2212	19	-0.29985294681625874
2212	20	-0.5820413854575577
2212	21	-0.589087737594177
2212	22	-0.4652866750998754
2212	23	-0.8928218111548247
2212	24	-0.5622613757501256
2212	25	-0.6729331013389057
2213	1	-0.3546881221255873
2213	2	-0.3240964410620606
2213	3	-0.24551861433033295
2213	4	-0.3621386359875558
2213	5	-0.3262380045954245
2213	6	-0.06695596392418679
2213	7	-0.7151622323467208
2213	8	-0.3334222109863276
2213	9	-0.5552121388024744
2213	10	-0.266549583257607
2213	11	-0.2906780808308653
2213	12	-0.4939538617200938
2213	13	-0.4027951510620582
2213	14	-0.2916031071635743
2213	15	-0.3977027263258677
2213	16	-0.6338750031720153
2213	17	-0.5333571077879782
2213	18	-0.6615578230863619
2213	19	-0.979248786086707
2213	20	-0.37970453735268705
2213	21	-1.210917318671413
2213	22	-0.6240213573455335
2213	23	-0.6859252594306895
2213	24	-0.8301439633819285
2213	25	-0.8917988750636141
2214	1	-1.1254172326541934
2214	2	-0.9801379072776477
2214	3	-0.8529542795269478
2214	4	-1.5268663940298168
2214	5	-1.3392371101486162
2214	6	-2.647787714810878
2214	7	-1.3551525636682555
2214	8	-1.7990979321634626
2214	9	-1.2817267965378392
2214	10	-1.5923153886782728
2214	11	-1.302475185306207
2214	12	-1.323333664904169
2214	13	-1.705864956475203
2214	14	-1.67700340267902
2214	15	-2.071120538326504
2214	16	-1.5073507520008178
2214	17	-1.7004136253519562
2214	18	-1.547157981848322
2214	19	-3.383945055086649
2214	20	-2.0304065981543786
2214	21	-2.522055956046117
2214	22	-2.189044630832754
2214	23	-2.1505579295942567
2214	24	-1.565311524025141
2214	25	-1.2230442253039655
2215	1	-1.1952416868959723
2215	2	-1.0813913939222317
2215	3	-0.9655700247578203
2215	4	-0.8219993206866718
2215	5	-1.513264579427215
2215	6	-1.2941817438579832
2215	7	-1.450410484022047
2215	8	-2.194417446758834
2215	9	-1.7310158677648886
2215	10	-1.131370017311518
2215	11	-1.6782852858418593
2215	12	-2.3291183336227093
2215	13	-1.8208646253746892
2215	14	-1.8031804693298525
2215	15	-2.946855436085876
2215	16	-2.379304768345463
2215	17	-1.9732379283202945
2215	18	-2.836763395962194
2215	19	-3.285972197343831
2215	20	-2.825692056697335
2215	21	-1.8735276088521289
2215	22	-2.7973054279856555
2215	23	-2.7372182155245
2215	24	-1.748597136358427
2215	25	-1.9332794663577246
2216	1	-1.1453036747163416
2216	2	-0.9595531836769311
2216	3	-1.3900968602401669
2216	4	-0.9690977241717746
2216	5	-0.8228385709580905
2216	6	-1.2235301462792876
2216	7	-2.612289180127707
2216	8	-1.604723836547958
2216	9	-1.5275649078944014
2216	10	-1.5630081602917514
2216	11	-2.0545186907816415
2216	12	-1.587333586673104
2216	13	-1.9688752205820848
2216	14	-2.145810710291812
2216	15	-2.21607639531976
2216	16	-1.959582938011458
2216	17	-2.1349637720308863
2216	18	-1.9787950596028239
2216	19	-2.0380693080080476
2216	20	-2.4111588643241717
2216	21	-2.175467908144128
2216	22	-2.617674745070019
2216	23	-1.748079579653698
2216	24	-2.1222047674308158
2216	25	-1.947315354464763
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64400.0
3	2.0
4	1016.0
5	9174.0
6	10574.0
7	8083.0
8	6500.0
9	5716.0
10	4867.0
11	4385.0
12	4169.0
13	3694.0
14	3800.0
15	3589.0
16	3802.0
17	3916.0
18	4477.0
19	5274.0
20	6562.0
21	8510.0
22	10890.0
23	13070.0
24	15574.0
25	17776.0
26	20252.0
27	22250.0
28	25295.0
29	30739.0
30	38400.0
31	49171.0
32	62693.0
33	80602.0
34	104586.0
35	136861.0
36	187486.0
37	293363.0
38	940776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.239392561731115	18.810275061691538	13.295424987390273	27.654907389187073
2	13.997209227899946	19.892410175695694	38.88195540279285	27.22842519361151
3	17.241560118139812	23.785491440106966	29.69546543090566	29.277483010847565
4	11.79089097593586	16.580071449532042	36.205994580470254	35.423042994061845
5	13.230358617101883	37.62636418126505	35.5652262420411	13.57805095959196
6	33.54280500542603	36.26604229271058	17.025695262143422	13.16545743971997
7	28.535085648757345	31.209455774745713	21.500456256777152	18.75500231971979
8	26.037160918157138	36.41252473308176	19.573310799501677	17.977003549259432
9	26.226272585423217	14.811469663734425	19.243798195427512	39.718459555414846
10	14.602915867752813	28.967481245794247	34.17059957796532	22.25900330848762
11	34.88518323805012	22.25301140008236	23.443286861765582	19.418518500101936
12	23.145011553201464	24.4989355502701	31.06312510143806	21.292927795090375
13	28.868350567892865	21.266338927501838	25.450867378651747	24.414443125953554
14	21.228396931381013	21.380833337100544	26.91845194772317	30.472317783795265
15	23.916578401682752	29.669918344153423	22.81341302271292	23.600090231450903
16	22.83711132883232	27.723947790915904	26.656490937919763	22.78244994233201
17	22.488037628452492	28.137053372389463	27.0700939354778	22.30481506368024
18	22.685783070880557	27.166970050233445	28.169921316149782	21.977325562736212
19	23.57285540121427	26.961974905489882	27.168240441515835	22.296929251780018
20	23.777740207184745	26.828417110732993	27.263110881267483	22.13073180081478
21	23.184131910433038	26.48790460920793	27.041087308213346	23.286876172145682
22	23.36237415743339	27.029120982439895	26.502155234761478	23.106349625365233
23	22.633338968509612	26.944565234096373	27.023442634658867	23.398653162735155
24	23.293930776800362	26.921931227011974	27.12172393078132	22.662414065406345
25	23.121316733877258	26.48281055835342	26.872286921486793	23.52358578628253
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	139.0
1	139.0
2	309.0
3	479.0
4	479.0
5	479.0
6	1107.5
7	1736.0
8	1736.0
9	1736.0
10	2437.5
11	3139.0
12	3139.0
13	3139.0
14	5319.5
15	7500.0
16	7500.0
17	7500.0
18	14014.0
19	20528.0
20	20528.0
21	20528.0
22	33066.5
23	45605.0
24	45605.0
25	45605.0
26	65777.0
27	85949.0
28	85949.0
29	85949.0
30	111458.0
31	136967.0
32	136967.0
33	136967.0
34	160260.5
35	183554.0
36	183554.0
37	183554.0
38	205368.0
39	227182.0
40	227182.0
41	227182.0
42	246284.0
43	265386.0
44	265386.0
45	265386.0
46	288297.5
47	311209.0
48	311209.0
49	311209.0
50	303310.5
51	295412.0
52	295412.0
53	295412.0
54	266520.0
55	237628.0
56	237628.0
57	237628.0
58	205576.0
59	173524.0
60	173524.0
61	173524.0
62	142233.0
63	110942.0
64	110942.0
65	110942.0
66	85798.5
67	60655.0
68	60655.0
69	60655.0
70	44277.5
71	27900.0
72	27900.0
73	27900.0
74	19483.5
75	11067.0
76	11067.0
77	11067.0
78	7531.0
79	3995.0
80	3995.0
81	3995.0
82	2656.0
83	1317.0
84	1317.0
85	1317.0
86	853.5
87	390.0
88	390.0
89	390.0
90	234.0
91	78.0
92	78.0
93	78.0
94	45.5
95	13.0
96	13.0
97	13.0
98	6.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.076798110920158
2	1.3560584623924308E-4
3	0.0
4	0.02996889201887272
5	0.07770214989508628
6	0.07471882127782292
7	0.03706559797205977
8	0.0764816972789331
9	0.05623122424053946
10	0.11404451668720342
11	0.005288628003330479
12	0.015956287907484268
13	0.0076391293381440255
14	0.010125236519196815
15	0.009266399493014943
16	0.022374964629475105
17	0.03507671222721754
18	0.0010396448211675302
19	0.004384589028402192
20	0.007322715696919126
21	2.2600974373207178E-4
22	2.7121169247848616E-4
23	0.0
24	4.5201948746414354E-5
25	0.004203781233416535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2212294.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.33487280599525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.90641266996484	54.60394546185415
2	13.16736617157665	17.995805850493102
3	3.700729999360866	7.58666741386967
4	1.3847441215264489	3.785052536534381
5	0.6574866900019133	2.246463466645779
6	0.34304749333296947	1.406526409399438
7	0.20779386865115387	0.99396973088996
8	0.12809519573304956	0.7002695125981605
9	0.09167357307747187	0.5638051760328137
>10	0.3790504133658589	4.356381375746439
>50	0.01856595719368563	0.8696461460690894
>100	0.011393909954066986	1.6070850578647318
>500	0.0022501424159139757	0.9596632395870377
>1k	0.0012574325265377018	1.6987667421559949
>5k	1.32361318582916E-4	0.6259518802593277
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6949	0.3141083418388334	No Hit
CCCATGTACTCTGCGTTGATACCAC	6892	0.31153183076028773	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4065	0.18374592165417436	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3026	0.13678109690664983	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2531	0.11440613227717474	No Hit
GTCCTACAGTGGACATTTCTAAATT	2406	0.10875588868387294	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2401	0.10852987894014088	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2393	0.10816826335016956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.5201948746414354E-5	0.0
10	0.0	0.0	0.0	4.5201948746414354E-5	0.0
11	0.0	0.0	0.0	9.040389749282871E-5	0.0
12	0.0	0.0	0.0	9.040389749282871E-5	0.0
13	0.0	0.0	0.0	1.8080779498565741E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	60	2.5339608E-5	14.270902	5
TAGGACC	395	0.0	12.7655325	4
TGGCGAG	1180	0.0	11.018876	18
GGCGAGG	570	0.0	10.822741	19
AGGACGT	1240	0.0	10.741539	5
GACGTGA	735	0.0	10.732198	7
GGACGTG	1255	0.0	10.609308	6
TTAGGAC	870	0.0	10.599324	3
CGTGAAA	785	0.0	10.409465	9
TAGGACG	1325	0.0	10.339673	4
GTAGGAC	1120	0.0	10.2705345	3
ACGTGAA	820	0.0	10.198523	8
TATGGCG	1300	0.0	10.150054	16
ATGGCGA	1355	0.0	9.948234	17
CCCCTAC	220	1.8189894E-12	9.9394045	1
TCTATAC	260	0.0	9.872249	3
TGTAGGA	1200	0.0	9.823498	2
TTTAGGA	1005	0.0	9.743094	2
GGCGAGA	750	0.0	9.617253	19
ATATGGC	2310	0.0	9.616139	15
>>END_MODULE