FastQCFastQC Report
Thu 26 May 2016
SRR1512996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174281
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC32180.2740400296010921No Hit
CCCATGTACTCTGCGTTGATACCAC26600.22652159065845398No Hit
GTATCAACGCAGAGTACTTTTTTTT20440.17406395913754885No Hit
GAGTACATGGGAAGCAGTGGTATCA12880.10968413863461982No Hit
GGTATCAACGCAGAGTACTTTTTTT12700.10815128576550247No Hit
TATCAACGCAGAGTACTTTTTTTTT12500.10644811591092762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCC456.7699E-414.7750945
GACCGTG500.001505079413.2930547
GCTAGAC701.0923125E-412.2120683
GCTTAGA907.213097E-611.6457151
TAGGACG1406.8030204E-1011.5336194
ACTATAC855.3299038E-511.174443
TCTGTAC1701.0913936E-1111.174443
GGACTTA600.005897091211.0775456
TAGTACA951.3620371E-510.9980024
GTGCTAG1803.274181E-1110.5870131
GCGCCAC1106.0254406E-610.36572913
AGGACGT1659.295036E-1010.3617545
CTTATAC1106.0530783E-610.3617543
CCTTAAT1201.4649995E-610.3223371
AAGGCGT1201.5322639E-610.2862926
TAGGGTG1303.8459802E-710.2289115
GTATAAT2151.8189894E-1210.1930781
CCCTAAA750.00258219510.1635321
TTAGGAC1502.4472683E-810.1314933
TAATACC750.002650737510.1314934
Produced by FastQC (version 0.11.5)