##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512996_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1174281 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.977864752985017 31.0 30.0 34.0 23.0 34.0 2 29.117786117632832 31.0 30.0 34.0 22.0 34.0 3 29.277809996074193 31.0 30.0 34.0 23.0 34.0 4 32.72359171271612 35.0 33.0 37.0 25.0 37.0 5 32.82275537115903 35.0 35.0 37.0 26.0 37.0 6 32.86823085786111 37.0 35.0 37.0 26.0 37.0 7 32.698659009215 37.0 33.0 37.0 25.0 37.0 8 32.690730753541956 37.0 35.0 37.0 25.0 37.0 9 33.99668818621778 39.0 35.0 39.0 24.0 39.0 10 33.881059984790696 38.0 34.0 39.0 23.0 39.0 11 33.93342223879974 39.0 34.0 39.0 23.0 39.0 12 33.83421600111047 38.0 34.0 39.0 23.0 39.0 13 33.82269916655383 39.0 34.0 39.0 23.0 39.0 14 34.82620343852962 39.0 34.0 41.0 22.0 41.0 15 34.74410554202955 39.0 34.0 41.0 21.0 41.0 16 34.62064190768649 39.0 34.0 41.0 18.0 41.0 17 34.55876659845472 39.0 34.0 41.0 18.0 41.0 18 34.256476942060715 39.0 34.0 41.0 17.0 41.0 19 34.50220262441443 39.0 34.0 41.0 18.0 41.0 20 34.578607675675585 39.0 34.0 41.0 18.0 41.0 21 34.524794320950434 39.0 34.0 41.0 18.0 41.0 22 34.433689210674444 39.0 34.0 41.0 18.0 41.0 23 34.16517937359116 39.0 34.0 41.0 11.0 41.0 24 34.16344214033949 39.0 34.0 41.0 10.0 41.0 25 34.08752249248689 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24171084355625538 1101 2 0.5824251331290071 1101 3 0.5530231914163615 1101 4 0.42955470992504985 1101 5 0.2456164507141665 1101 6 0.5008103101357193 1101 7 0.5082780081979266 1101 8 0.35259929368010745 1101 9 0.20357873536931237 1101 10 0.21422625487466007 1101 11 0.38551671168479373 1101 12 0.6546000763318958 1101 13 0.47856929238409407 1101 14 0.4315139324941768 1101 15 0.28031278120514713 1101 16 0.5296782222868401 1101 17 0.7713495701281943 1101 18 0.8513401650505514 1101 19 0.23159082150473154 1101 20 0.4513625735744142 1101 21 0.4429134682526481 1101 22 0.5162380576738528 1101 23 0.7364080081339068 1101 24 0.24418287763928248 1101 25 0.7559781655155433 1102 1 0.4285450575527783 1102 2 0.4569959449347323 1102 3 0.5817172672450113 1102 4 0.7324227586821905 1102 5 0.5962005913649833 1102 6 0.5575301665943044 1102 7 0.6917129961074622 1102 8 0.6682548190052202 1102 9 0.5434090056829888 1102 10 0.7674888263288224 1102 11 0.6935674452962743 1102 12 0.7652835347904343 1102 13 0.6803376187740326 1102 14 0.6606406707874299 1102 15 0.9292851692487218 1102 16 0.8792327436250389 1102 17 0.8507213602884391 1102 18 1.1107653200030114 1102 19 1.0736298012736754 1102 20 1.035598033367073 1102 21 0.9069842630730491 1102 22 1.2212916540840837 1102 23 1.3638695672628955 1102 24 1.2476954421449022 1102 25 1.0598159217236756 1103 1 0.18071600553722078 1103 2 0.3433310653247119 1103 3 0.29794270745418316 1103 4 0.30952640521395125 1103 5 -0.13641277934326013 1103 6 0.3278225603904872 1103 7 0.20593984552446187 1103 8 0.21654405110933794 1103 9 0.491918766879472 1103 10 0.5259687787718832 1103 11 0.5004918997024674 1103 12 0.6912144579072219 1103 13 0.7222323332656515 1103 14 0.6619611529775966 1103 15 0.8342211598229952 1103 16 0.8286851381922133 1103 17 0.6133509188355788 1103 18 0.9242961627160184 1103 19 1.0227933786055559 1103 20 0.8875094435753539 1103 21 0.6173137319461119 1103 22 0.9033990286222107 1103 23 1.086709350490878 1103 24 1.026500295620636 1103 25 1.0048999556070797 1104 1 0.27958780809330364 1104 2 0.3740802208935854 1104 3 0.4158217545321321 1104 4 0.6565079931815063 1104 5 0.3125561093791376 1104 6 0.2960140938108182 1104 7 0.4859267758326169 1104 8 0.43568199254962536 1104 9 0.5496730691598017 1104 10 0.6060581022072142 1104 11 0.6179942566174361 1104 12 0.5564030839454617 1104 13 0.5965468508212908 1104 14 0.8327932636287727 1104 15 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2216 24 -3.34749896439288 2216 25 -3.2480836882453765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56652.0 3 3.0 4 466.0 5 2788.0 6 3865.0 7 3281.0 8 3063.0 9 2701.0 10 2420.0 11 2381.0 12 2285.0 13 2267.0 14 2157.0 15 2299.0 16 2288.0 17 2386.0 18 2785.0 19 3257.0 20 4171.0 21 5308.0 22 6822.0 23 8163.0 24 9502.0 25 10790.0 26 12021.0 27 13403.0 28 15249.0 29 18259.0 30 22120.0 31 28214.0 32 35750.0 33 44887.0 34 58561.0 35 76322.0 36 105123.0 37 161984.0 38 440288.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4141076980135 17.873898263370812 12.160420715126842 25.551573323488846 2 15.685765057254281 19.983797404517986 39.103456383328535 25.226981154899196 3 18.856124049233298 24.22292471128807 29.40300071299517 27.51795052648347 4 12.756128601389188 16.413526622722806 36.84461148185314 33.98573329403487 5 13.669637160218388 37.36424282230111 35.27688969646787 13.689230321012644 6 32.99310022936776 35.89251995363245 17.697089763452254 13.417290053547534 7 28.3057050555362 31.482694389485044 21.919105629082384 18.292494925896367 8 26.17076604780591 35.042958538856034 20.397618818454923 18.388656594883134 9 26.726754094953698 15.021544767036293 19.433558732167754 38.81814240584225 10 15.198288351746442 28.553420443192202 33.9166303969174 22.33166080814396 11 34.51970601737697 22.608367551527447 23.55770418275369 19.3142222483419 12 23.01701339352256 24.929071822498564 30.71771394502055 21.33620083895833 13 28.735556800973015 21.385133236009068 25.389027342212895 24.490282620805022 14 21.86573673415151 21.1380486408766 27.064494476964068 29.931720148007823 15 24.393584607692237 28.63084747812799 23.22117157826862 23.754396335911153 16 23.496845146431543 27.6877580779242 25.786328513607437 23.029068262036823 17 22.836145959587785 27.577422105172605 26.710168467979674 22.876263467259943 18 22.894793375184562 27.228646154790443 27.36998782654359 22.506572643481405 19 23.63147911071589 27.215565623501416 27.116845240680977 22.036110025101724 20 23.98887946277993 26.739713332720065 26.892262981625905 22.3791442228741 21 23.669694450501527 26.532347088966418 26.950484490841692 22.847473969690363 22 23.764755182572895 26.58994837655728 26.295249297534497 23.350047143335328 23 23.31714430269342 26.698783522962472 26.543252990087268 23.440819184256835 24 23.233324645813212 26.89554023599393 26.625205051026406 23.245930067166455 25 23.43723249762575 26.321339289896983 26.563747013555584 23.677681198921686 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 256.0 1 256.0 2 357.0 3 458.0 4 458.0 5 458.0 6 836.5 7 1215.0 8 1215.0 9 1215.0 10 1708.5 11 2202.0 12 2202.0 13 2202.0 14 3598.5 15 4995.0 16 4995.0 17 4995.0 18 8651.0 19 12307.0 20 12307.0 21 12307.0 22 18607.0 23 24907.0 24 24907.0 25 24907.0 26 34516.0 27 44125.0 28 44125.0 29 44125.0 30 55991.0 31 67857.0 32 67857.0 33 67857.0 34 80312.0 35 92767.0 36 92767.0 37 92767.0 38 105246.0 39 117725.0 40 117725.0 41 117725.0 42 127944.5 43 138164.0 44 138164.0 45 138164.0 46 150027.0 47 161890.0 48 161890.0 49 161890.0 50 159089.0 51 156288.0 52 156288.0 53 156288.0 54 142782.0 55 129276.0 56 129276.0 57 129276.0 58 112670.0 59 96064.0 60 96064.0 61 96064.0 62 79304.0 63 62544.0 64 62544.0 65 62544.0 66 48682.0 67 34820.0 68 34820.0 69 34820.0 70 25586.0 71 16352.0 72 16352.0 73 16352.0 74 11444.5 75 6537.0 76 6537.0 77 6537.0 78 4475.5 79 2414.0 80 2414.0 81 2414.0 82 1594.5 83 775.0 84 775.0 85 775.0 86 510.5 87 246.0 88 246.0 89 246.0 90 156.0 91 66.0 92 66.0 93 66.0 94 43.0 95 20.0 96 20.0 97 20.0 98 15.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.347702125811454 2 0.03363760462785313 3 0.030657057382347152 4 0.030571898889618414 5 0.03423371407695432 6 0.015669162662088544 7 0.015498845676631063 8 0.020608355240355588 9 0.01822391744395081 10 0.017712966487578356 11 0.016861381560290937 12 0.017542649502120873 13 0.01694654005301968 14 0.024610804398606467 15 0.024866279876792692 16 0.018479392922137038 17 0.019586453327610682 18 0.10534105550545397 19 0.02197089112401546 20 0.020182562776711876 21 0.02256700057311666 22 0.017202015531205903 23 0.020012245791254393 24 0.015584004169359804 25 0.019245819356695715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1174281.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.46351111393659 #Duplication Level Percentage of deduplicated Percentage of total 1 82.12015365285737 60.3283482055487 2 12.221616584191866 17.95685731526102 3 3.2007974038805433 7.054254469603133 4 1.0859994802743804 3.191253395554653 5 0.4939971868838433 1.8145383914447317 6 0.2655485837760596 1.1704878801313607 7 0.14183846155859417 0.7293965977965418 8 0.10155591543326624 0.5968523297694208 9 0.06661672941156774 0.44045089573507323 >10 0.2823137466194811 3.523199336608571 >50 0.011075305536992167 0.5356018244328292 >100 0.006626799339982571 1.0776822096823158 >500 0.001162593897529773 0.5792386139079596 >1k 6.975563385178637E-4 1.0018385345237932 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3218 0.2740400296010921 No Hit CCCATGTACTCTGCGTTGATACCAC 2660 0.22652159065845398 No Hit GTATCAACGCAGAGTACTTTTTTTT 2044 0.17406395913754885 No Hit GAGTACATGGGAAGCAGTGGTATCA 1288 0.10968413863461982 No Hit GGTATCAACGCAGAGTACTTTTTTT 1270 0.10815128576550247 No Hit TATCAACGCAGAGTACTTTTTTTTT 1250 0.10644811591092762 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTCC 45 6.7699E-4 14.775094 5 GACCGTG 50 0.0015050794 13.293054 7 GCTAGAC 70 1.0923125E-4 12.212068 3 GCTTAGA 90 7.213097E-6 11.645715 1 TAGGACG 140 6.8030204E-10 11.533619 4 ACTATAC 85 5.3299038E-5 11.17444 3 TCTGTAC 170 1.0913936E-11 11.17444 3 GGACTTA 60 0.0058970912 11.077545 6 TAGTACA 95 1.3620371E-5 10.998002 4 GTGCTAG 180 3.274181E-11 10.587013 1 GCGCCAC 110 6.0254406E-6 10.365729 13 AGGACGT 165 9.295036E-10 10.361754 5 CTTATAC 110 6.0530783E-6 10.361754 3 CCTTAAT 120 1.4649995E-6 10.322337 1 AAGGCGT 120 1.5322639E-6 10.286292 6 TAGGGTG 130 3.8459802E-7 10.228911 5 GTATAAT 215 1.8189894E-12 10.193078 1 CCCTAAA 75 0.002582195 10.163532 1 TTAGGAC 150 2.4472683E-8 10.131493 3 TAATACC 75 0.0026507375 10.131493 4 >>END_MODULE