FastQCFastQC Report
Thu 26 May 2016
SRR1512996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174281
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC31890.27157043331195857No Hit
CCCATGTACTCTGCGTTGATACCAC31180.26552418032821784No Hit
GTATCAACGCAGAGTACTTTTTTTT26690.22728801709301266No Hit
TATCAACGCAGAGTACTTTTTTTTT21290.18130243101949192No Hit
GGTATCAACGCAGAGTACTTTTTTT17890.1523485434917196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC550.003054812312.0976933
ACTAATA657.983189E-411.6988692
CTAATAC657.983189E-411.6988693
GCGTGCG752.0646665E-411.4049389
TAGATAG855.241666E-511.1927665
CTTATAC855.289731E-511.1827431
AAGGTAT600.005821925611.096656
TAGGACG1553.0377123E-1011.0483434
GTCTAAA951.3508694E-511.0061721
GGACGTG1752.0008883E-1110.8701896
GTCTTAT1053.4400418E-610.86323451
TCGGTCA700.001500948310.84843215
CCATCGC700.001503082710.84658319
TCGAACT1605.529728E-1010.67710619
ATAATAC909.455655E-510.5614793
CTAGACC1002.3587587E-510.4652374
TGAGTAG750.00261494210.1481085
CTCCTAG856.556471E-410.0644681
AGGACGT1803.8198777E-1010.0423985
TAGGACC951.6190598E-410.0145814
Produced by FastQC (version 0.11.5)