##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512996_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1174281 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.35012318175973 31.0 31.0 34.0 26.0 34.0 2 30.46576586013058 31.0 31.0 34.0 27.0 34.0 3 30.5605574815568 31.0 31.0 34.0 27.0 34.0 4 34.05197563445206 37.0 35.0 37.0 30.0 37.0 5 33.70485599273087 37.0 35.0 37.0 28.0 37.0 6 33.56436747252148 37.0 35.0 37.0 28.0 37.0 7 33.428660601678814 37.0 35.0 37.0 28.0 37.0 8 33.30862119032838 37.0 35.0 37.0 27.0 37.0 9 34.74750506905928 39.0 35.0 39.0 27.0 39.0 10 34.638201588887156 39.0 35.0 39.0 27.0 39.0 11 34.677974862916116 39.0 35.0 39.0 27.0 39.0 12 34.52144418584649 39.0 35.0 39.0 27.0 39.0 13 34.49876307289311 39.0 35.0 39.0 27.0 39.0 14 35.55031376646646 40.0 36.0 41.0 27.0 41.0 15 35.55701573984421 40.0 36.0 41.0 27.0 41.0 16 35.51779429284814 40.0 36.0 41.0 27.0 41.0 17 35.49792341015481 40.0 36.0 41.0 27.0 41.0 18 35.4216290649342 40.0 36.0 41.0 26.0 41.0 19 35.390286481685386 40.0 36.0 41.0 25.0 41.0 20 35.36719235004228 40.0 36.0 41.0 25.0 41.0 21 35.31753813610201 40.0 36.0 41.0 25.0 41.0 22 35.28723874438912 40.0 36.0 41.0 25.0 41.0 23 35.176232094362426 40.0 35.0 41.0 25.0 41.0 24 35.15328017740217 40.0 36.0 41.0 25.0 41.0 25 35.05676494808313 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5785666668264433 1101 2 0.5751926176719984 1101 3 0.5474277554014897 1101 4 0.3628986930603446 1101 5 0.6063027253320215 1101 6 0.6546395648315126 1101 7 0.635857895387872 1101 8 0.5300008928501825 1101 9 0.3496388660668899 1101 10 0.6577087700426958 1101 11 0.7098804098937563 1101 12 0.5181022307385419 1101 13 0.6847581681200623 1101 14 0.684470242079513 1101 15 0.5937631779644406 1101 16 0.9523392307022576 1101 17 0.9050806015081463 1101 18 0.3662429092537707 1101 19 0.644841769899756 1101 20 0.7990561849891975 1101 21 0.9185694865129506 1101 22 0.8841640470153891 1101 23 0.7326385591508782 1101 24 0.6800383835671227 1101 25 0.7229264463707352 1102 1 0.4678137229613881 1102 2 0.4110223815142646 1102 3 0.3672147285863012 1102 4 0.3467746816832715 1102 5 0.5631430231619206 1102 6 0.4124755113308396 1102 7 0.51549496538901 1102 8 0.4226691015781654 1102 9 0.5656717743554154 1102 10 0.8206020188405958 1102 11 0.7213482813859002 1102 12 0.7490340454811815 1102 13 0.7976395271928496 1102 14 0.8930447412441396 1102 15 0.8176853048972959 1102 16 0.8138251684000934 1102 17 0.7959597926362463 1102 18 0.7617856520434998 1102 19 0.970390164169217 1102 20 0.8796740801063763 1102 21 0.9683182581653753 1102 22 0.9661268707787798 1102 23 0.901327199676615 1102 24 1.0037332160146448 1102 25 1.0607913605639823 1103 1 0.568670904368286 1103 2 0.514956200758121 1103 3 0.5867797710537559 1103 4 0.5602656896462435 1103 5 0.6136949119011987 1103 6 0.6618315620978095 1103 7 0.7237875690672055 1103 8 0.6780260646894121 1103 9 0.6911769368105212 1103 10 0.8382266011034858 1103 11 0.746610610960019 1103 12 0.7436520709197438 1103 13 0.9024957870769583 1103 14 0.9766646736719196 1103 15 0.664419115452155 1103 16 0.851487670163138 1103 17 0.9192514035052 1103 18 0.9264732781524572 1103 19 0.9533646329091852 1103 20 1.0669209634655203 1103 21 0.6815426303014931 1103 22 0.8852916994480537 1103 23 0.9164338096445448 1103 24 1.004157263353754 1103 25 0.9559265340669612 1104 1 0.437164414267329 1104 2 0.504080052737617 1104 3 0.4774459715403303 1104 4 0.5299202793349238 1104 5 0.5889103641062903 1104 6 0.5758567150111276 1104 7 0.6796024372984917 1104 8 0.505803738409135 1104 9 0.7034328946545187 1104 10 0.6482186718796115 1104 11 0.7747520716437677 1104 12 0.7605365310977632 1104 13 0.8950660418735126 1104 14 1.0182088119775656 1104 15 0.8635570985156633 1104 16 0.7980455337263734 1104 17 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-1.8277868698765403 2216 16 -1.550614564167958 2216 17 -1.9472296549812498 2216 18 -1.4785424236268767 2216 19 -1.643210330473714 2216 20 -2.098669053444091 2216 21 -2.0385464738561936 2216 22 -2.1625071465399657 2216 23 -1.3244064113729053 2216 24 -1.660589413587111 2216 25 -1.57279564381723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32301.0 3 1.0 4 518.0 5 4438.0 6 5256.0 7 4217.0 8 3238.0 9 2789.0 10 2444.0 11 2217.0 12 2182.0 13 1972.0 14 1896.0 15 1835.0 16 1914.0 17 2059.0 18 2258.0 19 2687.0 20 3453.0 21 4494.0 22 5680.0 23 6841.0 24 8233.0 25 9458.0 26 10880.0 27 12107.0 28 13660.0 29 16556.0 30 20845.0 31 26343.0 32 33737.0 33 43591.0 34 56294.0 35 74135.0 36 101756.0 37 157335.0 38 494661.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.41829515025797 18.808452831217796 13.114825350993964 27.658426667530268 2 14.500192032401104 20.52907268362513 37.81812019439981 27.152615089573963 3 17.162757466057954 24.37900298139883 29.85094709017688 28.607292462366335 4 12.079786835308468 16.943798938265378 36.00229315596868 34.97412107045747 5 13.373393147504883 37.60375724809359 35.40060950459003 13.622240099811489 6 33.024762904732356 36.404435778033836 17.227587594002905 13.343213723230903 7 28.06556617315692 31.61077730553731 21.932431845307313 18.39122467599846 8 26.276978509230176 35.21493326424835 20.08003858759845 18.428049638923024 9 26.442889775567046 14.683952630596872 19.324187654270496 39.54896993956559 10 14.7839520382221 28.688059174685023 34.12877450905166 22.399214278041217 11 34.696783500975535 22.47989528430193 23.32070374808278 19.502617466639755 12 22.735720016420725 24.969636989556488 30.828377874265623 21.466265119757164 13 28.754809600294323 21.167862076248454 25.508552955423795 24.568775368033425 14 21.723215494767825 20.97877917894832 27.090975210722064 30.207030115561796 15 24.27248215377645 28.61689425463167 23.1903818606402 23.92024173095168 16 23.352336076849106 27.576294183933953 25.83103997219859 23.24032976701836 17 22.79413956982964 27.609086580112397 26.793008304746458 22.80376554531151 18 22.91736745606423 27.106434545347707 27.425866771809915 22.550331226778145 19 23.43397113376777 27.059282397866202 27.31135947894986 22.195386989416168 20 23.868538107716585 26.5533196333538 27.04002943252959 22.538112826400024 21 23.551623122253638 26.385615015158226 27.09677419354839 22.96598766903975 22 23.551708280818882 26.400773239772455 26.522039036686312 23.52547944272235 23 23.061260464914273 26.42672409755416 26.89662866043136 23.6153867771002 24 23.15308103689069 26.85228395271996 26.78901113874033 23.20562387164901 25 23.322546306152862 26.1808814136683 26.799744517777302 23.696827762401533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 151.0 1 151.0 2 248.5 3 346.0 4 346.0 5 346.0 6 835.5 7 1325.0 8 1325.0 9 1325.0 10 1733.5 11 2142.0 12 2142.0 13 2142.0 14 3472.0 15 4802.0 16 4802.0 17 4802.0 18 8487.5 19 12173.0 20 12173.0 21 12173.0 22 18684.0 23 25195.0 24 25195.0 25 25195.0 26 35169.0 27 45143.0 28 45143.0 29 45143.0 30 56863.5 31 68584.0 32 68584.0 33 68584.0 34 80833.5 35 93083.0 36 93083.0 37 93083.0 38 105271.5 39 117460.0 40 117460.0 41 117460.0 42 127521.5 43 137583.0 44 137583.0 45 137583.0 46 149520.5 47 161458.0 48 161458.0 49 161458.0 50 159430.0 51 157402.0 52 157402.0 53 157402.0 54 143673.0 55 129944.0 56 129944.0 57 129944.0 58 113000.5 59 96057.0 60 96057.0 61 96057.0 62 78947.0 63 61837.0 64 61837.0 65 61837.0 66 48064.5 67 34292.0 68 34292.0 69 34292.0 70 25035.0 71 15778.0 72 15778.0 73 15778.0 74 10983.5 75 6189.0 76 6189.0 77 6189.0 78 4264.0 79 2339.0 80 2339.0 81 2339.0 82 1517.0 83 695.0 84 695.0 85 695.0 86 466.0 87 237.0 88 237.0 89 237.0 90 147.0 91 57.0 92 57.0 93 57.0 94 33.0 95 9.0 96 9.0 97 9.0 98 4.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07655748496313915 2 0.0 3 0.0 4 0.03006094793324596 5 0.07400273018127689 6 0.07774970386134153 7 0.03781037077156149 8 0.07212924334124456 9 0.05211699754999016 10 0.11470848970561559 11 0.004598558607352074 12 0.013199566372955026 13 0.00570561901282572 14 0.008856483243789178 15 0.007068154896485593 16 0.020438038254898105 17 0.031849276280549545 18 8.51584927287421E-4 19 0.0031508642309634573 20 0.0059610944910119465 21 8.51584927287421E-5 22 8.51584927287421E-5 23 0.0 24 8.51584927287421E-5 25 0.002639913274591005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1174281.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.20999736697748 #Duplication Level Percentage of deduplicated Percentage of total 1 81.2121987137046 58.6433265528306 2 12.80636099921134 18.494945880672276 3 3.3256658021595094 7.204389564521556 4 1.1938411855337008 3.448290754559112 5 0.5278157855089658 1.9056788240925784 6 0.2818973296404557 1.2213483258657172 7 0.15651895879536065 0.7911563521746533 8 0.10286781184415715 0.5942467537930657 9 0.0755765451220195 0.4911643911839654 >10 0.29510331855307115 3.564595565264546 >50 0.01257223961830124 0.5953739594973332 >100 0.0075704269918102675 1.1462755933516378 >500 0.0013011597931868537 0.7017714717201508 >1k 7.097235235564658E-4 1.1974360104728328 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3189 0.27157043331195857 No Hit CCCATGTACTCTGCGTTGATACCAC 3118 0.26552418032821784 No Hit GTATCAACGCAGAGTACTTTTTTTT 2669 0.22728801709301266 No Hit TATCAACGCAGAGTACTTTTTTTTT 2129 0.18130243101949192 No Hit GGTATCAACGCAGAGTACTTTTTTT 1789 0.1523485434917196 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.51584927287421E-5 0.0 10 0.0 0.0 0.0 8.51584927287421E-5 0.0 11 0.0 0.0 0.0 8.51584927287421E-5 0.0 12 0.0 0.0 0.0 8.51584927287421E-5 0.0 13 0.0 0.0 0.0 8.51584927287421E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAGAC 55 0.0030548123 12.097693 3 ACTAATA 65 7.983189E-4 11.698869 2 CTAATAC 65 7.983189E-4 11.698869 3 GCGTGCG 75 2.0646665E-4 11.404938 9 TAGATAG 85 5.241666E-5 11.192766 5 CTTATAC 85 5.289731E-5 11.182743 1 AAGGTAT 60 0.0058219256 11.09665 6 TAGGACG 155 3.0377123E-10 11.048343 4 GTCTAAA 95 1.3508694E-5 11.006172 1 GGACGTG 175 2.0008883E-11 10.870189 6 GTCTTAT 105 3.4400418E-6 10.8632345 1 TCGGTCA 70 0.0015009483 10.848432 15 CCATCGC 70 0.0015030827 10.846583 19 TCGAACT 160 5.529728E-10 10.677106 19 ATAATAC 90 9.455655E-5 10.561479 3 CTAGACC 100 2.3587587E-5 10.465237 4 TGAGTAG 75 0.002614942 10.148108 5 CTCCTAG 85 6.556471E-4 10.064468 1 AGGACGT 180 3.8198777E-10 10.042398 5 TAGGACC 95 1.6190598E-4 10.014581 4 >>END_MODULE