FastQCFastQC Report
Thu 26 May 2016
SRR1512995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597116
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29440.18433225889666124No Hit
GGTATCAACGCAGAGTACTTTTTTT17740.1110752130715615No Hit
TATCAACGCAGAGTACTTTTTTTTT17610.11026124589572704No Hit
GTCCTACAGTGGACATTTCTAAATT16130.10099454266315033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAC350.002174326516.2811224
TAGGACC3950.012.9836814
GGTATCA7100.012.7460171
GTGTTAC855.1768315E-511.2070251
CTAAGCA1451.2223609E-911.134794
ATAGGAC2250.010.9750253
TTAGACC1158.847255E-710.7361024
GTCCTAA5800.010.6756581
CTTATAC1252.2472705E-710.6373343
ATTAGAC1002.4051282E-510.447383
GTAGGAC9450.010.1508943
CTGCGTG750.002657979710.1285729
CAGTCCG856.6216965E-410.0540989
ACAGATC1803.9835868E-1010.0224388
GTATCAA14050.09.8988741
TGGCGAG6550.09.86506818
TGTAGGA10500.09.7689792
GTCTTAA2053.8198777E-119.7583111
GTATAAT2251.8189894E-129.7376591
CCCCTAG900.00108608179.5259711
Produced by FastQC (version 0.11.5)