##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512995_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1597116
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.16216793269869	31.0	30.0	34.0	24.0	34.0
2	29.35791827268652	31.0	30.0	34.0	23.0	34.0
3	29.481771518161487	31.0	30.0	34.0	24.0	34.0
4	32.9738553743122	35.0	35.0	37.0	26.0	37.0
5	33.09428745313427	37.0	35.0	37.0	27.0	37.0
6	33.1161086608612	37.0	35.0	37.0	26.0	37.0
7	32.962161170509845	37.0	35.0	37.0	26.0	37.0
8	32.9352426498764	37.0	35.0	37.0	26.0	37.0
9	34.29454028386166	39.0	35.0	39.0	25.0	39.0
10	34.182141434936476	39.0	35.0	39.0	25.0	39.0
11	34.23950358020332	39.0	35.0	39.0	26.0	39.0
12	34.13995289008438	39.0	35.0	39.0	25.0	39.0
13	34.135162380190295	39.0	35.0	39.0	25.0	39.0
14	35.15681453319608	40.0	35.0	41.0	25.0	41.0
15	35.09132022971406	40.0	35.0	41.0	25.0	41.0
16	34.98054493224036	39.0	35.0	41.0	24.0	41.0
17	34.92680306252019	39.0	35.0	41.0	23.0	41.0
18	34.62074514312047	39.0	34.0	41.0	18.0	41.0
19	34.866064831859426	39.0	34.0	41.0	22.0	41.0
20	34.938892353467125	40.0	35.0	41.0	23.0	41.0
21	34.88765812877712	39.0	35.0	41.0	22.0	41.0
22	34.80112840895715	39.0	35.0	41.0	22.0	41.0
23	34.53064586417017	39.0	34.0	41.0	18.0	41.0
24	34.53557161784116	39.0	34.0	41.0	18.0	41.0
25	34.457887216708116	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6750248236842147
1101	2	0.9100085132200455
1101	3	0.9176176444338822
1101	4	0.9567254767937854
1101	5	0.8652484112938197
1101	6	1.1358616627024318
1101	7	1.110640342008999
1101	8	0.8941313123195513
1101	9	0.7087120934179651
1101	10	0.8615106533733652
1101	11	1.1093486927964662
1101	12	1.3565341322372575
1101	13	1.0813469404986407
1101	14	1.156334217704817
1101	15	0.8607652498200267
1101	16	1.3472703949936928
1101	17	1.3640951795526632
1101	18	1.5568533026226277
1101	19	1.1512636342945726
1101	20	1.2745700136096758
1101	21	1.175023910169827
1101	22	1.2130329434743459
1101	23	1.4136408364361728
1101	24	1.1213434289691477
1101	25	1.386821223965562
1102	1	0.8081050493760742
1102	2	0.8717261087479713
1102	3	0.839966813413966
1102	4	0.9743576320735556
1102	5	0.8537758822298045
1102	6	0.8653227690842655
1102	7	1.0529683429048973
1102	8	1.0344191275358199
1102	9	1.0349725585264125
1102	10	1.1671951344058726
1102	11	1.1735718518930582
1102	12	1.1551140013386743
1102	13	1.0839241589584887
1102	14	1.1446004977213562
1102	15	1.3300224386748667
1102	16	1.4452683522819854
1102	17	1.3289639062764067
1102	18	1.6089513763606433
1102	19	1.5565467300553095
1102	20	1.4454666764788868
1102	21	1.2600658884847533
1102	22	1.5709647020840904
1102	23	1.7613923800475675
1102	24	1.6618843518268918
1102	25	1.4496902656003385
1103	1	0.22908625092978596
1103	2	0.39671922069900134
1103	3	0.3933081099042077
1103	4	0.35766803249607904
1103	5	0.05477348428943429
1103	6	0.4730007566784167
1103	7	0.43024342255690584
1103	8	0.3854242921417921
1103	9	0.5110434355283431
1103	10	0.4794417993229345
1103	11	0.6216300761417699
1103	12	0.6825832135993224
1103	13	0.7438203435925388
1103	14	0.8135396891257258
1103	15	0.9639765548666475
1103	16	0.9558432447916303
1103	17	0.8353690151239306
1103	18	1.1813017258320428
1103	19	1.0993954635945116
1103	20	0.975977422244064
1103	21	0.8224389092437363
1103	22	0.9759002242227268
1103	23	1.2949023090577398
1103	24	1.027539108913814
1103	25	1.0809393611606737
1104	1	0.5544824132787909
1104	2	0.4918543690385029
1104	3	0.540885943866563
1104	4	0.7778407390049367
1104	5	0.5177847334191839
1104	6	0.5163957709077707
1104	7	0.7111550784141443
1104	8	0.6681675652599139
1104	9	0.6971775701026175
1104	10	0.9206001787642606
1104	11	0.7659032797620142
1104	12	0.7144198386161165
1104	13	0.735338915038561
1104	14	0.8420848749623246
1104	15	1.0286689442990635
1104	16	0.897828026099269
1104	17	0.9256283191679984
1104	18	1.1900122897542147
1104	19	1.0264981714469172
1104	20	0.9684457573484337
1104	21	1.10245161184411
1104	22	0.7764811239088232
1104	23	1.1578666562784363
1104	24	1.1246089610018046
1104	25	0.9956824388541747
1105	1	0.7419953455090109
1105	2	0.8715642301700122
1105	3	0.878921713517844
1105	4	1.0089629465407128
1105	5	0.6762573432003549
1105	6	0.4975708496434379
1105	7	0.8405343313636706
1105	8	0.8932963980139519
1105	9	0.8866095310693112
1105	10	1.0106302043839506
1105	11	0.9343239974395985
1105	12	1.069092249314373
1105	13	1.113842039987567
1105	14	1.0654410538758512
1105	15	1.3315430814704499
1105	16	1.1390057157583797
1105	17	1.2080102972688778
1105	18	1.1634498968428773
1105	19	1.167912965591917
1105	20	1.2568602740828183
1105	21	1.3104677995695013
1105	22	0.8828865350476818
1105	23	1.169712742340998
1105	24	1.2180506476583517
1105	25	1.1974542767811016
1106	1	0.49421012518041607
1106	2	0.6265930602989336
1106	3	0.6032223514203636
1106	4	0.5775333169583874
1106	5	0.6319349310476383
1106	6	0.5098432895726859
1106	7	0.3877822193891092
1106	8	0.5081060808506024
1106	9	0.5334797220569456
1106	10	0.6890938107361535
1106	11	0.6593947158621418
1106	12	0.7236249330386002
1106	13	0.7031474350209947
1106	14	0.8095332719759369
1106	15	0.8734910786864134
1106	16	0.8466729975529361
1106	17	0.8539989823212721
1106	18	1.1329898918622519
1106	19	0.7952201740819618
1106	20	0.7768603379369239
1106	21	0.7702952657747204
1106	22	0.8011012380941551
1106	23	1.097866656278434
1106	24	0.7451184032503804
1106	25	0.9440179680592706
1107	1	0.6155450014842359
1107	2	0.6876149576562369
1107	3	0.6175300933678578
1107	4	0.8158614484542213
1107	5	0.6400795814283171
1107	6	0.509893860120151
1107	7	0.718235616923252
1107	8	0.7327618598542074
1107	9	0.9006501292894669
1107	10	0.6285071923855625
1107	11	0.7002470312486224
1107	12	0.88409365686136
1107	13	0.7779930862641606
1107	14	1.0097389669021268
1107	15	1.0721911363926822
1107	16	1.0243527711954385
1107	17	1.0833903983586453
1107	18	1.3128147254137943
1107	19	1.22996803491678
1107	20	1.211910070812742
1107	21	1.1281619332687143
1107	22	1.0926370314933038
1107	23	1.0982733923994559
1107	24	0.9775908996980291
1107	25	1.095312015626611
1108	1	0.7675103672369765
1108	2	0.8852534763910995
1108	3	0.6957461740756301
1108	4	0.8267366751101761
1108	5	0.7347262415234823
1108	6	0.9151930459841182
1108	7	1.0061233659405744
1108	8	0.9987853712265604
1108	9	1.0097826910798346
1108	10	1.0248660937278515
1108	11	0.9449800944202238
1108	12	1.0360219057995792
1108	13	1.1346576841247753
1108	14	1.3057047950938738
1108	15	1.2965829682595995
1108	16	1.2871209553921972
1108	17	1.3723983450077881
1108	18	1.4769537978585134
1108	19	1.5354199473454386
1108	20	1.4401535327771384
1108	21	1.331927768762121
1108	22	1.2742635998308387
1108	23	1.6015664448619447
1108	24	1.5773103939357966
1108	25	1.6246136110411626
1109	1	-0.37693611987887365
1109	2	-0.31095634681568995
1109	3	0.042748043119967605
1109	4	0.08543845529830918
1109	5	-0.03842080017765426
1109	6	-0.056559872482651485
1109	7	0.024145855752749412
1109	8	0.06739461840000871
1109	9	0.2595666785786861
1109	10	0.18593175540019047
1109	11	-0.16511121299161147
1109	12	0.2256012176235771
1109	13	0.19682332434905447
1109	14	-0.1106248307908615
1109	15	0.25412348975361
1109	16	0.2624832742327783
1109	17	0.03542190722245664
1109	18	-1.5768915310626497
1109	19	-0.16130156504847548
1109	20	0.07646508101992566
1109	21	0.10152056221740224
1109	22	0.29082455825226106
1109	23	-0.17090804727887843
1109	24	0.18385358111599004
1109	25	0.38789148584583444
1110	1	0.27830876555200845
1110	2	0.2007651978403402
1110	3	0.3091993622072309
1110	4	0.19212920560948277
1110	5	0.23423046752159138
1110	6	0.2352017363622423
1110	7	0.4204757668084014
1110	8	0.3242146849582568
1110	9	0.663760802189941
1110	10	0.847789078269237
1110	11	0.6436422891631466
1110	12	0.13321141337499398
1110	13	0.35399649118932786
1110	14	0.488566192615302
1110	15	0.6036546125817353
1110	16	0.8062204753091535
1110	17	0.46152209538875155
1110	18	0.9407956390116823
1110	19	0.7682050023090596
1110	20	0.6476004022796431
1110	21	0.753490769477267
1110	22	0.8380696836990182
1110	23	0.7164908114148503
1110	24	0.8124220864171079
1110	25	0.9673385877310352
1111	1	0.10842892687288241
1111	2	0.3491052923687228
1111	3	0.2935829580613749
1111	4	0.11251790716228527
1111	5	0.24118080870952951
1111	6	0.3732515534023264
1111	7	0.3453347606995507
1111	8	0.12600408018894171
1111	9	0.5512976252799433
1111	10	0.4949356034961667
1111	11	0.4444028125346762
1111	12	0.47682094216258974
1111	13	0.41688566002142835
1111	14	0.4530811816414513
1111	15	0.5621731493451136
1111	16	0.4618773297080452
1111	17	0.5651602018716844
1111	18	0.6409907972966025
1111	19	0.5868292353903115
1111	20	0.4019722113154174
1111	21	0.6118860442741365
1111	22	0.3219819471188998
1111	23	0.5981069446244476
1111	24	0.6980793476880507
1111	25	0.21310957363814254
1112	1	-0.21633343753705958
1112	2	0.014387391571524688
1112	3	0.12700870998932956
1112	4	0.17822923939343838
1112	5	0.12090827654272118
1112	6	0.29283710734910784
1112	7	-0.08606325880419519
1112	8	0.14038201747436574
1112	9	0.3818395747646264
1112	10	0.33338961155369873
1112	11	0.3113422852010146
1112	12	0.36038720458348195
1112	13	0.00969788939753613
1112	14	0.3311913240687758
1112	15	0.16880880443892465
1112	16	0.5013400696113095
1112	17	0.4977339912736696
1112	18	0.4584349781769035
1112	19	0.26548807043681677
1112	20	0.14805725695993033
1112	21	0.283788053180551
1112	22	0.2044220718497769
1112	23	0.507035265447044
1112	24	0.0724722088650509
1112	25	0.34032890350064093
1113	1	-0.362703733907356
1113	2	-0.21438661220247823
1113	3	-0.4119134789328598
1113	4	-0.11595435479015492
1113	5	-0.3282397976053488
1113	6	-0.28180769229568625
1113	7	-0.403751126492061
1113	8	-0.08784253575018397
1113	9	-0.27603310810805937
1113	10	-0.5777547995907142
1113	11	-0.4402727664140329
1113	12	-0.2587547645584891
1113	13	-0.34132053662089135
1113	14	-0.39388915101170596
1113	15	-0.6597293240992101
1113	16	-0.7200219017506626
1113	17	-0.7489748554351792
1113	18	-0.589583669841744
1113	19	-0.6721381921894505
1113	20	-0.7981631892605137
1113	21	-0.5764517070592134
1113	22	-0.4137208962931922
1113	23	-0.9433671099553322
1113	24	-0.288774877382032
1113	25	-0.26685002867829155
1114	1	-1.1345750392176477
1114	2	-1.053740245890502
1114	3	-0.8034675754790328
1114	4	-1.0883753041524677
1114	5	-1.0873931948332327
1114	6	-1.5275482653342003
1114	7	-1.6716466312338412
1114	8	-1.1929534707739577
1114	9	-1.1357688513224033
1114	10	-1.2823305548796213
1114	11	-1.9098197188452986
1114	12	-1.5366595767739213
1114	13	-1.393221651002193
1114	14	-1.801946404552318
1114	15	-1.781195628529062
1114	16	-2.420855801079014
1114	17	-4.325674727142971
1114	18	-3.0998671704580474
1114	19	-2.1684271515132707
1114	20	-1.4176070048216047
1114	21	-2.477439053926119
1114	22	-2.465384921649772
1114	23	-2.4304001640644586
1114	24	-1.9939650517085
1114	25	-1.8118585814919186
1115	1	-1.3533140853120038
1115	2	-1.2002972901192663
1115	3	-1.2096359759425255
1115	4	-1.1839400011872065
1115	5	-1.1301026948348465
1115	6	-1.1425817846889252
1115	7	-2.1095620727307036
1115	8	-1.527506486870351
1115	9	-1.4550304572174255
1115	10	-1.1760543502324268
1115	11	-1.906273477628595
1115	12	-1.6869560382791136
1115	13	-1.4563004933014554
1115	14	-1.4229244854441845
1115	15	-2.7954969509055303
1115	16	-1.5620983249879785
1115	17	-1.709628526272148
1115	18	-1.876639564840609
1115	19	-1.8455428086185037
1115	20	-1.5954185476156724
1115	21	-2.348041918160625
1115	22	-2.2830691651322965
1115	23	-1.6562344361118662
1115	24	-1.5817993173005647
1115	25	-2.0620533713867246
1116	1	-1.6190663432856454
1116	2	-2.461929549763507
1116	3	-1.9469633573482383
1116	4	-1.8160060521880546
1116	5	-2.3621757885678782
1116	6	-2.254543537739618
1116	7	-1.7817615425603783
1116	8	-2.569054839730885
1116	9	-2.6975138556141367
1116	10	-3.638719434741116
1116	11	-2.263804656049661
1116	12	-2.099401364975897
1116	13	-2.71456665452925
1116	14	-2.546190642577365
1116	15	-2.1924824977450257
1116	16	-2.071262639300201
1116	17	-2.531093299917913
1116	18	-2.2306639539859603
1116	19	-2.59323791072584
1116	20	-2.938923715425144
1116	21	-3.521763518957272
1116	22	-2.6873236305111234
1116	23	-2.7215177791494263
1116	24	-3.2427656292919664
1116	25	-3.111255700074313
1201	1	0.39859143108846595
1201	2	0.5465626359178231
1201	3	0.588586468853272
1201	4	0.6511469923276394
1201	5	0.7022987884431018
1201	6	0.6780946677711412
1201	7	0.6676722235497081
1201	8	0.05426272740771054
1201	9	0.5037392394274463
1201	10	0.7111771657392794
1201	11	0.22995880209056452
1201	12	-0.3527110093370993
1201	13	-0.0572066059609071
1201	14	0.6544674174928957
1201	15	0.3447036332942801
1201	16	0.7881626220588629
1201	17	0.8464159653668304
1201	18	1.1468563886377865
1201	19	0.7517653165824782
1201	20	0.5951247180076535
1201	21	0.5798087357143871
1201	22	0.7889106826541763
1201	23	1.0722461419243672
1201	24	0.9068665699863843
1201	25	0.8253518301354106
1202	1	0.7925286739309136
1202	2	0.9376724850520581
1202	3	0.5960346623214718
1202	4	0.9704656855386773
1202	5	0.870722251926729
1202	6	0.9991317627480001
1202	7	0.9927032967538949
1202	8	1.0199750538263572
1202	9	1.3817715555597943
1202	10	1.4114908443780578
1202	11	1.0952256566251037
1202	12	1.1149862519349725
1202	13	1.1130665167889617
1202	14	1.3414464055779263
1202	15	1.080618899880939
1202	16	1.20932508134225
1202	17	1.419774175121674
1202	18	1.7623254868719727
1202	19	1.3372809174739615
1202	20	1.2961442405518042
1202	21	1.1997995209021113
1202	22	1.3392921330227665
1202	23	1.547814835135405
1202	24	1.555850529784088
1202	25	1.3632906052082703
1203	1	0.4535152323116094
1203	2	0.7303936075777422
1203	3	0.8442729272535487
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2211	14	0.45370131638485844
2211	15	0.5948149312760762
2211	16	0.4479308749278772
2211	17	0.39901353659753624
2211	18	0.4246932505293586
2211	19	0.13826610803479866
2211	20	0.005622590979555753
2211	21	0.02390004952523128
2211	22	-0.050622360479977146
2211	23	0.4452458698626316
2211	24	0.2883620438684602
2211	25	0.3243439650780289
2212	1	-0.0233032099437267
2212	2	-0.007888692646304207
2212	3	0.15861664897591154
2212	4	-0.09250544124415683
2212	5	-0.033742186263076235
2212	6	-0.17177918250990132
2212	7	-0.19319325933225429
2212	8	-0.028570691089996103
2212	9	0.030518748880695057
2212	10	3.8050631157915404E-4
2212	11	-0.10362305151188878
2212	12	-0.04640001035907204
2212	13	-0.1122829345250338
2212	14	-0.13267632931344053
2212	15	-0.143511108657691
2212	16	0.03941869002002818
2212	17	0.024571145489467483
2212	18	-2.928375471351991
2212	19	-1.0941608459111336
2212	20	-0.43876420636638613
2212	21	-0.4253899101721714
2212	22	0.03394900661943012
2212	23	-0.0820980392202415
2212	24	-0.40930885796456096
2212	25	-0.13996530067706203
2213	1	-0.48064820045906487
2213	2	-0.3329998235037124
2213	3	-0.5334835368538933
2213	4	-0.5696219499619275
2213	5	-0.5859275988168307
2213	6	-0.47134764012254493
2213	7	-0.4030370618504193
2213	8	-0.6999495791246417
2213	9	-0.621057868244236
2213	10	-0.6899270593952878
2213	11	-0.6919683935971292
2213	12	-0.7788480572144536
2213	13	-0.4582138027481406
2213	14	-0.6150833854371314
2213	15	-0.7537338339811512
2213	16	-1.2215804638941847
2213	17	-0.9395704251393795
2213	18	-0.7109835018265329
2213	19	-0.5996716846950889
2213	20	-1.3242939758369872
2213	21	-1.0770839748224006
2213	22	-0.9583090379565462
2213	23	-1.4718291075677499
2213	24	-1.0426248574854924
2213	25	-1.1864470132474239
2214	1	-1.930232497637352
2214	2	-3.99991755969441
2214	3	-2.1063677014158273
2214	4	-1.6383167288979088
2214	5	-1.6194338590766186
2214	6	-1.756942955161957
2214	7	-1.9141924419641754
2214	8	-1.5937460265099404
2214	9	-1.8746258853969309
2214	10	-1.9749126497259795
2214	11	-1.7151595041344478
2214	12	-2.1257261800925313
2214	13	-2.117207078780531
2214	14	-3.3464966279518293
2214	15	-3.3502446267294985
2214	16	-2.702269872807669
2214	17	-3.1581955457646984
2214	18	-2.451573174747061
2214	19	-4.547514646672678
2214	20	-2.883124566008341
2214	21	-2.753369017446751
2214	22	-2.694316378839396
2214	23	-2.910813575194407
2214	24	-3.066546951304005
2214	25	-2.9379860737876413
2215	1	-0.8931377319519385
2215	2	-2.5486791179604644
2215	3	-1.2584888708368993
2215	4	-0.8957767922397082
2215	5	-0.9345419229752707
2215	6	-1.56992573267706
2215	7	-1.8180758835410842
2215	8	-1.8297503488961162
2215	9	-1.524764546390962
2215	10	-3.1385991731676235
2215	11	-1.983804463232211
2215	12	-2.3684481968439
2215	13	-1.5506177996903716
2215	14	-2.9353745477058624
2215	15	-2.7808872209081983
2215	16	-2.4468324688878127
2215	17	-2.694444254439091
2215	18	-1.6007958328108316
2215	19	-2.206246639206121
2215	20	-2.316962771929468
2215	21	-2.0396288037212216
2215	22	-1.5975365225850524
2215	23	-2.1693474442138836
2215	24	-1.9928984821774023
2215	25	-1.9823564034756984
2216	1	-2.1830888811578646
2216	2	-2.256491953713823
2216	3	-2.6842991290383935
2216	4	-3.442522964726834
2216	5	-2.591319817719551
2216	6	-2.5972355822233695
2216	7	-2.4752018632408372
2216	8	-3.2288075211607747
2216	9	-3.3001332622728228
2216	10	-2.74613183499568
2216	11	-3.5228574412454776
2216	12	-3.792389480081493
2216	13	-4.398968729981881
2216	14	-3.6769948762808298
2216	15	-3.5109907623969114
2216	16	-2.798650893989489
2216	17	-2.8465730610367146
2216	18	-3.0411653473925746
2216	19	-3.336932550950042
2216	20	-3.0918669150192635
2216	21	-3.4684755000417375
2216	22	-4.03008188003642
2216	23	-3.9226456784064716
2216	24	-3.8610028890535624
2216	25	-3.9491351408612765
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68987.0
3	1.0
4	594.0
5	3480.0
6	4390.0
7	4068.0
8	3553.0
9	3391.0
10	3101.0
11	3036.0
12	3042.0
13	2928.0
14	2897.0
15	2814.0
16	3047.0
17	3204.0
18	3591.0
19	4278.0
20	5514.0
21	7089.0
22	8910.0
23	10857.0
24	12620.0
25	14560.0
26	15980.0
27	17767.0
28	20188.0
29	23764.0
30	29699.0
31	36974.0
32	46859.0
33	59854.0
34	78108.0
35	102857.0
36	141860.0
37	220249.0
38	623005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04555616574698	18.803558435323968	13.018713328298206	25.132172070630844
2	15.104606441752377	19.929594227227742	40.79262869097879	24.173170640041093
3	18.59411084184608	23.937835063234132	29.723769156425167	27.74428493849462
4	12.353541009769987	16.437731518889183	38.2345498527424	32.974177618598425
5	13.334302156236474	37.57587981751887	35.84934970894597	13.240468317298687
6	32.4100840167733	36.91972315045365	17.711532391797718	12.958660440975336
7	28.06834065620256	31.6817633001508	22.061917907045487	18.187978136601153
8	25.051355535625714	36.40637760598029	20.525930787768864	18.016336070625137
9	26.05484822485392	15.270139093294588	20.197210618936978	38.477802062914506
10	14.649966338400839	28.83299149848914	34.81655211285247	21.70049005025755
11	34.33974765639336	22.614293291983735	24.445340686414188	18.60061836520872
12	22.735331564355494	24.853220138791908	31.604310928437812	20.80713736841479
13	28.45194704342489	21.952429263893585	25.891732110873132	23.70389158180839
14	20.932992702832877	21.881292483560863	27.848547612590636	29.337167201015628
15	22.997906229390328	30.441407930774673	23.495450761943353	23.06523507789165
16	22.50685451395891	28.36917053179004	26.952299597058538	22.171675357192512
17	21.785240775406344	28.49292024923328	27.58732339765409	22.134515577706285
18	21.716198963203176	27.755280349434752	28.965252702773004	21.56326798458907
19	23.120460150786563	27.390032153938076	27.594204024064723	21.895303671210637
20	23.290945016267468	27.41502243626878	27.86644079048314	21.427591756980608
21	23.054414636498805	27.037025902724803	27.260053135721563	22.648506325054832
22	23.028599985971805	27.67894718383952	27.351038086553974	21.941414743634706
23	22.504213269403113	27.472457962813007	27.541410911350056	22.48191785643382
24	22.89075817600886	26.929883780496816	28.019997419849375	22.159360623644954
25	22.7091448826985	27.224532484938063	27.754925661034356	22.311396971329085
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158.0
1	158.0
2	316.0
3	474.0
4	474.0
5	474.0
6	871.0
7	1268.0
8	1268.0
9	1268.0
10	2077.5
11	2887.0
12	2887.0
13	2887.0
14	4916.0
15	6945.0
16	6945.0
17	6945.0
18	12774.0
19	18603.0
20	18603.0
21	18603.0
22	29613.5
23	40624.0
24	40624.0
25	40624.0
26	57309.0
27	73994.0
28	73994.0
29	73994.0
30	94505.5
31	115017.0
32	115017.0
33	115017.0
34	132234.0
35	149451.0
36	149451.0
37	149451.0
38	163151.5
39	176852.0
40	176852.0
41	176852.0
42	186300.0
43	195748.0
44	195748.0
45	195748.0
46	199894.5
47	204041.0
48	204041.0
49	204041.0
50	198477.5
51	192914.0
52	192914.0
53	192914.0
54	175412.5
55	157911.0
56	157911.0
57	157911.0
58	136713.5
59	115516.0
60	115516.0
61	115516.0
62	94746.0
63	73976.0
64	73976.0
65	73976.0
66	57135.0
67	40294.0
68	40294.0
69	40294.0
70	29494.5
71	18695.0
72	18695.0
73	18695.0
74	13168.0
75	7641.0
76	7641.0
77	7641.0
78	5199.5
79	2758.0
80	2758.0
81	2758.0
82	1853.5
83	949.0
84	949.0
85	949.0
86	617.0
87	285.0
88	285.0
89	285.0
90	183.5
91	82.0
92	82.0
93	82.0
94	50.5
95	19.0
96	19.0
97	19.0
98	16.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35251039999599276
2	0.04107403594979952
3	0.03687897435126816
4	0.037192038649666026
5	0.042388906003070534
6	0.01828295502643515
7	0.01828295502643515
8	0.02529559531054726
9	0.02166404944913206
10	0.0213509851507342
11	0.02034917939586104
12	0.02110053371201591
13	0.021037920852336335
14	0.029365431189719467
15	0.029741108347796905
16	0.02241540376528693
17	0.02373027381855795
18	0.1017458969793052
19	0.026422626784779565
20	0.02322937094112137
21	0.028175786855807593
22	0.020787469413618045
23	0.02347982237983966
24	0.019409986500667453
25	0.023542435239519235
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1597116.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.5443618788572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.82612753978152	63.32591313768494
2	11.357593726889457	17.160043411543512
3	2.7403218186071645	6.210475893881632
4	0.9187036552832045	2.7761152557657316
5	0.4042818339972871	1.5270606584269568
6	0.20611542507244004	0.9342514956292134
7	0.12903383338929475	0.6823445022923935
8	0.08725322053074154	0.5273191093496058
9	0.055315359725872634	0.37608871973314373
>10	0.24966767199748763	3.2599288763962333
>50	0.015624255174504623	0.816263823284552
>100	0.00879961018204154	1.358723248901953
>500	4.980211581358089E-4	0.23928031217490875
>1k	6.640282108477452E-4	0.8061915549352208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2944	0.18433225889666124	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1774	0.1110752130715615	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1761	0.11026124589572704	No Hit
GTCCTACAGTGGACATTTCTAAATT	1613	0.10099454266315033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	6.261285967957243E-5	0.0
13	0.0	0.0	0.0	6.261285967957243E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGAC	35	0.0021743265	16.281122	4
TAGGACC	395	0.0	12.983681	4
GGTATCA	710	0.0	12.746017	1
GTGTTAC	85	5.1768315E-5	11.207025	1
CTAAGCA	145	1.2223609E-9	11.13479	4
ATAGGAC	225	0.0	10.975025	3
TTAGACC	115	8.847255E-7	10.736102	4
GTCCTAA	580	0.0	10.675658	1
CTTATAC	125	2.2472705E-7	10.637334	3
ATTAGAC	100	2.4051282E-5	10.44738	3
GTAGGAC	945	0.0	10.150894	3
CTGCGTG	75	0.0026579797	10.128572	9
CAGTCCG	85	6.6216965E-4	10.054098	9
ACAGATC	180	3.9835868E-10	10.022438	8
GTATCAA	1405	0.0	9.898874	1
TGGCGAG	655	0.0	9.865068	18
TGTAGGA	1050	0.0	9.768979	2
GTCTTAA	205	3.8198777E-11	9.758311	1
GTATAAT	225	1.8189894E-12	9.737659	1
CCCCTAG	90	0.0010860817	9.525971	1
>>END_MODULE