FastQCFastQC Report
Thu 26 May 2016
SRR1512995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597116
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36100.22603242344325647No Hit
TATCAACGCAGAGTACTTTTTTTTT25220.15790963211188166No Hit
GGTATCAACGCAGAGTACTTTTTTT21690.1358072926449926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTAC602.54463E-514.2640251
GGTATCA7800.012.4353021
TAGGACC3750.012.432184
GCCTTAG1354.654794E-911.270341
CAACGCT600.00580978811.1001614
GTCCTAC7550.010.8318411
TGGCGAG8650.010.75167218
GTACCGT909.3901705E-510.569266
ACTAGCC1355.6221324E-810.5636243
TCTACAC1909.094947E-1210.5080263
GTCCTAA3900.010.4846671
GTAGGAC7950.010.4041723
GGCGAGA5100.010.2343119
GGCGAGG4000.010.20174719
TCCAACG750.00267087910.12266318
GTATCAA15450.010.0325181
CTAGGAC1909.822543E-1110.0076443
TGTACCG1054.0365325E-59.9674915
CCAATAC1252.5441896E-69.8875513
GCTAGAC1252.5441896E-69.8875513
Produced by FastQC (version 0.11.5)